AT5G24050

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000766
Physical Location & Seq
Reverse (-)
8128353 .. 8129402
1050 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G24050.1

Sequence Viewer

Length: 1050 bp
ATGACTAGTATCTATCACGATGATGACGATCATCTCACGGCTTCAGGTAAAGACCTGTGGTCGAATTTCTGTGTACTGGTTGATGCGGCGGTTATGTTATATGAAGAAGAAGAAGAAGAACAACGTCGTAAGATTGTCTCTGAAGAAGAAGAAGAATTCCAGAAGAGAATCTTTTGTCTCTTCCCGAGAAAAACAAGATCGTCTTTGGTGAAGAGACAACAAAATCTTATTAGGGTTTCTACTTCTTCATCGCTTATCGATCTTAACCAGTTCCCTACCGATTCTGAGATCGAAGACCCACAAAACCATCTTCAAGGGCTATCCTCTTCATGTTTCATTGCCGCTAATTCCGAGACAAAAACATTACAAAACCCTAGTTCAGAACCTTGTTCTTCACTCGGACTCTTTGGTCACAAGACGGCTGAGTACCAAAAGACGGAGACGAAAGACCCACCAAACCCTAATTTCCCATGTACTATGTCTTTGTGCCTAATGGAGAACACAAGCCGCAAGAGACGTGCTGTGGAACAAAGGAAGCGTAGTGGTGGAGTCAAGAAAGCAAATGTTGCTCCTTTTTCACAAACGGCGAGAGAGACACCTGAGTGGCTTGTCAAGGTGATGACAAACATGAAAGAAGCCAAAGACGCTAAATTGATCTTTGAGAAGACTCTGTTCGTGACTGATGTAAACCCAACAAAGAACCGTCTCTCAATGCCTTTCAACAACTTACTCCGAAACGATTTCCTGACGTCTGTGGAGTCCATAATCATAAACGAAGACATCAACAACAATAACAAGATTGGAGTGGGAGCGATTCTCGTGGATCAACGGTGTAAAAAGTGGGGTGTGATGTTAAAGAGATGGGAGATGAAGAAGGAATCAGGTAAAGGAAGCTGGAGTTACAATTTGATTTGTGGGTGGAACGACATCGTTGAGGCTAACAGATTGAAAGAAGGCGACAACATTACTCTTTGGTCGTTTAGGTGCTGTGGAATCCTCTGCTTTGCCATGGAACAGAGTAGCTCTTCCCTTGCTCTCTGTCTCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

349

Amino Acids

39.78

Weight (kDa)

6.49

Isoelectric Point (pI)

60.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 197 - 313 2.4e-44 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 408
AatII GACGTC 1 cut(s) 752
AciI CCGC 4 cut(s) 86, 89, 342, 508
AclWI GGATC 1 cut(s) 831
AcsI RAATTY 2 cut(s) 64, 155
AcuI CTGAAG 2 cut(s) 27, 162
AcyI GRCGYC 1 cut(s) 749
AfaI GTAC 3 cut(s) 75, 428, 475
AfiI CCNNNNNNNGG 1 cut(s) 436
AgsI TTSAA 3 cut(s) 314, 721, 949
AhdI GACNNNNNGTC 1 cut(s) 58
AhlI ACTAGT 1 cut(s) 5
AjiI CACGTC 1 cut(s) 518
AleI CACNNNNGTG 1 cut(s) 601
AluBI AGCT 2 cut(s) 894, 1023
AluI AGCT 2 cut(s) 894, 1023
Alw26I GTCTC 9 cut(s) 142, 182, 208, 347, 434, 508, 587, 710, 1046
AlwI GGATC 1 cut(s) 831
Ama87I CYCGRG 1 cut(s) 184
ApoI RAATTY 2 cut(s) 64, 155
AsuHPI GGTGA 2 cut(s) 220, 628
AvaI CYCGRG 1 cut(s) 184
BauI CACGAG 1 cut(s) 818
BbsI GAAGAC 3 cut(s) 300, 671, 783
BccI CCATC 2 cut(s) 315, 855
BceAI ACGGC 3 cut(s) 54, 435, 600
BcoDI GTCTC 9 cut(s) 142, 182, 208, 347, 434, 508, 587, 710, 1046
BcuI ACTAGT 1 cut(s) 5
BfaI CTAG 2 cut(s) 6, 375
BisI GCNGC 3 cut(s) 87, 342, 508
BlsI GCNGC 3 cut(s) 88, 343, 509
BmeRI GACNNNNNGTC 1 cut(s) 58
BmeT110I CYCGRG 1 cut(s) 184
BmgBI CACGTC 1 cut(s) 518
BmsI GCATC 1 cut(s) 73
BpiI GAAGAC 3 cut(s) 300, 671, 783
BplI GAGNNNNNCTC 2 cut(s) 801, 833
BpmI CTGGAG 1 cut(s) 916
Bsa29I ATCGAT 1 cut(s) 258
BsaBI GATNNNNATC 1 cut(s) 27
BsaHI GRCGYC 1 cut(s) 749
BsaJI CCNNGG 1 cut(s) 1008
Bsc4I CCNNNNNNNGG 1 cut(s) 436
Bse1I ACTGG 2 cut(s) 81, 268
Bse3DI GCAATG 1 cut(s) 336
Bse8I GATNNNNATC 1 cut(s) 27
BseCI ATCGAT 1 cut(s) 258
BseDI CCNNGG 1 cut(s) 1008
BseJI GATNNNNATC 1 cut(s) 27
BseLI CCNNNNNNNGG 1 cut(s) 436
BseMI GCAATG 1 cut(s) 336
BseMII CTCAG 3 cut(s) 276, 414, 591
BseNI ACTGG 2 cut(s) 81, 268
BshVI ATCGAT 1 cut(s) 258
BsiHKCI CYCGRG 1 cut(s) 184
BslI CCNNNNNNNGG 1 cut(s) 436
BsmAI GTCTC 9 cut(s) 142, 182, 208, 347, 434, 508, 587, 710, 1046
BsmBI CGTCTC 3 cut(s) 434, 508, 710
BsoBI CYCGRG 1 cut(s) 184
Bsp143I GATC 6 cut(s) 28, 197, 259, 288, 654, 823
Bsp19I CCATGG 1 cut(s) 1008
BspACI CCGC 4 cut(s) 86, 89, 342, 508
BspCNI CTCAG 3 cut(s) 277, 415, 592
BspDI ATCGAT 1 cut(s) 258
BspPI GGATC 1 cut(s) 831
BspQI GCTCTTC 1 cut(s) 1030
BsrDI GCAATG 1 cut(s) 336
BsrI ACTGG 2 cut(s) 81, 268
BssECI CCNNGG 1 cut(s) 1008
BssMI GATC 6 cut(s) 28, 197, 259, 288, 654, 823
BssNI GRCGYC 1 cut(s) 749
BssSI CACGAG 1 cut(s) 818
BssT1I CCWWGG 1 cut(s) 1008
Bst2BI CACGAG 1 cut(s) 818
Bst4CI ACNGT 2 cut(s) 704, 831
Bst6I CTCTTC 5 cut(s) 158, 185, 206, 331, 1030
BstACI GRCGYC 1 cut(s) 749
BstAPI GCANNNNNTGC 1 cut(s) 566
BstDEI CTNAG 3 cut(s) 285, 423, 600
BstDSI CCRYGG 1 cut(s) 1008
BstKTI GATC 6 cut(s) 31, 200, 262, 291, 657, 826
BstMAI GTCTC 9 cut(s) 142, 182, 208, 347, 434, 508, 587, 710, 1046
BstMBI GATC 6 cut(s) 28, 197, 259, 288, 654, 823
BstMWI GCNNNNNNNGC 2 cut(s) 566, 644
BstV2I GAAGAC 3 cut(s) 300, 671, 783
Bsu15I ATCGAT 1 cut(s) 258
BsuTUI ATCGAT 1 cut(s) 258
BtgI CCRYGG 1 cut(s) 1008
BtgZI GCGATG 1 cut(s) 234
BtrI CACGTC 1 cut(s) 518
ClaI ATCGAT 1 cut(s) 258
CseI GACGC 1 cut(s) 653
Csp6I GTAC 3 cut(s) 74, 427, 474
CviAII CATG 4 cut(s) 330, 471, 628, 1009
CviJI RGCY 9 cut(s) 41, 319, 422, 507, 607, 638, 894, 938, 1023
CviKI_1 RGCY 9 cut(s) 41, 319, 422, 507, 607, 638, 894, 938, 1023
CviQI GTAC 3 cut(s) 74, 427, 474
DdeI CTNAG 3 cut(s) 285, 423, 600
DpnI GATC 6 cut(s) 30, 199, 261, 290, 656, 825
DpnII GATC 6 cut(s) 28, 197, 259, 288, 654, 823
DrdI GACNNNNNNGTC 1 cut(s) 408
DriI GACNNNNNGTC 1 cut(s) 58
DseDI GACNNNNNNGTC 1 cut(s) 408
Eam1104I CTCTTC 5 cut(s) 158, 185, 206, 331, 1030
Eam1105I GACNNNNNGTC 1 cut(s) 58
EarI CTCTTC 5 cut(s) 158, 185, 206, 331, 1030
Eco130I CCWWGG 1 cut(s) 1008
Eco57I CTGAAG 2 cut(s) 27, 162
Eco88I CYCGRG 1 cut(s) 184
EcoRI GAATTC 1 cut(s) 155
EcoT14I CCWWGG 1 cut(s) 1008
ErhI CCWWGG 1 cut(s) 1008
Esp3I CGTCTC 3 cut(s) 434, 508, 710
FaeI CATG 4 cut(s) 333, 474, 631, 1012
FalI AAGNNNNNCTT 4 cut(s) 155, 187, 187, 219
FatI CATG 4 cut(s) 329, 470, 627, 1008
Fnu4HI GCNGC 3 cut(s) 87, 342, 508
Fsp4HI GCNGC 3 cut(s) 87, 342, 508
FspBI CTAG 2 cut(s) 6, 375
GluI GCNGC 3 cut(s) 87, 342, 508
GsuI CTGGAG 1 cut(s) 916
HgaI GACGC 1 cut(s) 653
Hin1I GRCGYC 1 cut(s) 749
Hin1II CATG 4 cut(s) 333, 474, 631, 1012
HinfI GANTC 9 cut(s) 168, 281, 402, 549, 667, 758, 814, 878, 993
HphI GGTGA 2 cut(s) 220, 628
Hpy166II GTNNAC 2 cut(s) 74, 688
Hpy188I TCNGA 6 cut(s) 142, 286, 352, 382, 401, 734
Hpy188III TCNNGA 6 cut(s) 17, 160, 184, 553, 676, 745
Hpy8I GTNNAC 2 cut(s) 74, 688
Hpy99I CGWCG 1 cut(s) 129
HpyAV CCTTC 2 cut(s) 868, 947
HpyCH4III ACNGT 2 cut(s) 704, 831
HpyCH4IV ACGT 3 cut(s) 124, 517, 749
HpyF10VI GCNNNNNNNGC 2 cut(s) 566, 644
HpyF3I CTNAG 3 cut(s) 285, 423, 600
HpySE526I ACGT 3 cut(s) 124, 517, 749
Hsp92I GRCGYC 1 cut(s) 749
Hsp92II CATG 4 cut(s) 333, 474, 631, 1012
Kzo9I GATC 6 cut(s) 28, 197, 259, 288, 654, 823
LguI GCTCTTC 1 cut(s) 1030
LmnI GCTCC 2 cut(s) 574, 809
LpnPI CCDG 9 cut(s) 30, 62, 68, 173, 281, 612, 758, 867, 880
LweI GCATC 1 cut(s) 73
MaeI CTAG 2 cut(s) 6, 375
MaeII ACGT 3 cut(s) 124, 517, 749
MaeIII GTNAC 3 cut(s) 410, 676, 899
MalI GATC 6 cut(s) 30, 199, 261, 290, 656, 825
MboI GATC 6 cut(s) 28, 197, 259, 288, 654, 823
MluCI AATT 6 cut(s) 64, 155, 346, 463, 650, 904
MlyI GAGTC 4 cut(s) 396, 558, 661, 767
MnlI CCTC 3 cut(s) 334, 928, 1007
MseI TTAA 2 cut(s) 264, 854
MslI CAYNNNNRTG 2 cut(s) 21, 601
MwoI GCNNNNNNNGC 2 cut(s) 566, 644
NcoI CCATGG 1 cut(s) 1008
NdeII GATC 6 cut(s) 28, 197, 259, 288, 654, 823
NlaIII CATG 4 cut(s) 333, 474, 631, 1012
NmuCI GTSAC 2 cut(s) 410, 676
OliI CACNNNNGTG 1 cut(s) 601
PciSI GCTCTTC 1 cut(s) 1030
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PfeI GAWTC 5 cut(s) 168, 281, 814, 878, 993
PkrI GCNGC 3 cut(s) 88, 343, 509
PleI GAGTC 4 cut(s) 396, 557, 661, 766
PpsI GAGTC 4 cut(s) 396, 557, 661, 766
RsaI GTAC 3 cut(s) 75, 428, 475
RsaNI GTAC 3 cut(s) 74, 427, 474
RseI CAYNNNNRTG 2 cut(s) 21, 601
SapI GCTCTTC 1 cut(s) 1030
SaqAI TTAA 2 cut(s) 264, 854
SatI GCNGC 3 cut(s) 87, 342, 508
Sau3AI GATC 6 cut(s) 28, 197, 259, 288, 654, 823
SchI GAGTC 4 cut(s) 396, 558, 661, 767
SfaNI GCATC 1 cut(s) 73
SmiMI CAYNNNNRTG 2 cut(s) 21, 601
SpeI ACTAGT 1 cut(s) 5
Sse9I AATT 6 cut(s) 64, 155, 346, 463, 650, 904
SsiI CCGC 4 cut(s) 86, 89, 342, 508
SspMI CTAG 2 cut(s) 6, 375
StyI CCWWGG 1 cut(s) 1008
TaaI ACNGT 2 cut(s) 704, 831
TaiI ACGT 3 cut(s) 127, 520, 752
TaqI TCGA 3 cut(s) 62, 258, 291
TasI AATT 6 cut(s) 64, 155, 346, 463, 650, 904
TatI WGTACW 2 cut(s) 73, 473
TauI GCSGC 3 cut(s) 89, 344, 510
TfiI GAWTC 5 cut(s) 168, 281, 814, 878, 993
Tru1I TTAA 2 cut(s) 264, 854
Tru9I TTAA 2 cut(s) 264, 854
TseFI GTSAC 2 cut(s) 410, 676
Tsp45I GTSAC 2 cut(s) 410, 676
TspDTI ATGAA 6 cut(s) 117, 237, 318, 325, 644, 884
TspGWI ACGGA 1 cut(s) 452
XapI RAATTY 2 cut(s) 64, 155
XspI CTAG 2 cut(s) 6, 375
ZraI GACGTC 1 cut(s) 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.