RLG00000026259

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000766
Physical Location & Seq
Forward (+)
1701606 .. 1702886
1281 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026259

Sequence Viewer

Length: 1212 bp
ATGGGGAGGCTGCTGAGTTTGAAAGATTTTGAGGGCCAAGCAATTCGACCTCGTTCGTCCTACCTTGATATTTTTCTTCAGGTTGCTAGTGTTCTTCAGAACAAGAAACTCAAGCAAAGTAAGAAGGAGAAAAGTACTCATTCGAAAACCAAGATGGCTGGTATAGGGTTTAAAGAACCCATTTCCATGGTAGGGAAAGAGACTTATGCAAAGGGACAAAAATTTATGAACTCTGTTTTTGAATCTGTGAGGAAGATTGGTGGGTCAAAAAGAAAGGGTTTTGACAATTTTGATGATCATGAAAGGGGTTTTCAATTCAAGAAGCAGAAGATGGATATGGGTAGTACCTCCGAGGCTAAAGAACCAAATTCGCCAGTCGTAAAAGAATTGAAGGTGGATGCGTTATACTCAGTTGGCACAATAAAGACAGAAAATTCTTTGCCTAGGGCAAACTATATAATGAGTTGTGATATTAAGAAGAAGAGTGCTAATGGTGATGATGATAAACAATGGGGTTTGAGGCCGAAGCCGAAGAATATCAAATTGAGGAAGCCAAAGAAGGAGATCACAACACAAGGTATTGATGCTAACGGCTTGCCTGAAGAATACAAGAGAAAGATTGAAAATTTTGATGAGGCTAAGTTGACTTTGGTGATACAGAAGGAGTTGACCAAGACTGATTTGTCACGTACCGCTTGCCTGTTGTCAATGCCTATGAAGCAAATCAAACCGGAGAGTTTTCTGAGTGCAGATGAGATAAAGTTTCTTGCAAAACAAAATGACTGGCCAGTTTCGGCTATAGATCCCCAACTTGAAGAAGAAGATCTAACCTTGAGGCAGTGGAACATGGAAAAGAACACGGGTAAGACGAGCTCAAGCTATGTGTTGAAAACACGTTGGAACGAAGTTCGCAGGAAAAATGGTCTCAAACGAAATGATCCAAAAGATGGTGGCTCAAGTAGTGATGGTGATAGGTGTAGCCTACCTTCTTCACGATCAACAACGCCGCCTAGCAAGAGTGATGCTGATGGTGAAAGTAATAGTTGTGATATTGGCAAAGGAACAGAAACAAAGGAACAAGAGATGATAGAAGGAGCTCAGATTGATGATGGGAGCAGTAGTGGGAATGGGAGAACTTCAACACCGTATGGCAATGAGTCTCATGATCGCGTTGGGCTGCTAAATGAGCAAATGCAACCACTTTTTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

404

Amino Acids

45.18

Weight (kDa)

9.23

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 213 - 303 1.5e-13 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 682
AccB7I CCANNNNNTGG 1 cut(s) 947
AccII CGCG 1 cut(s) 1170
AciI CCGC 2 cut(s) 693, 1007
AclWI GGATC 2 cut(s) 797, 932
AcoI YGGCCR 1 cut(s) 785
AcsI RAATTY 4 cut(s) 221, 367, 433, 625
AcuI CTGAAG 3 cut(s) 62, 80, 621
AfaI GTAC 3 cut(s) 136, 346, 691
AfiI CCNNNNNNNGG 2 cut(s) 192, 947
AflIII ACRYGT 1 cut(s) 893
AgsI TTSAA 9 cut(s) 22, 242, 314, 319, 391, 623, 815, 889, 1140
AluBI AGCT 3 cut(s) 873, 879, 1097
AluI AGCT 3 cut(s) 873, 879, 1097
Alw21I GWGCWC 2 cut(s) 875, 1099
Alw26I GTCTC 3 cut(s) 194, 929, 1164
AlwI GGATC 2 cut(s) 797, 932
AoxI GGCC 3 cut(s) 34, 521, 785
ApeKI GCWGC 2 cut(s) 10, 1177
ApoI RAATTY 4 cut(s) 221, 367, 433, 625
AspA2I CCTAGG 1 cut(s) 443
AspS9I GGNCC 1 cut(s) 34
AsuHPI GGTGA 4 cut(s) 506, 664, 980, 1043
AsuII TTCGAA 1 cut(s) 143
AvrII CCTAGG 1 cut(s) 443
BalI TGGCCA 1 cut(s) 787
BanII GRGCYC 2 cut(s) 875, 1099
BarI GAAGNNNNNNTAC 2 cut(s) 970, 1002
Bbv12I GWGCWC 2 cut(s) 875, 1099
BbvI GCAGC 1 cut(s) 1164
BccI CCATC 6 cut(s) 148, 325, 941, 959, 1022, 1103
BceAI ACGGC 1 cut(s) 607
BclI TGATCA 1 cut(s) 295
BcoDI GTCTC 3 cut(s) 194, 929, 1164
BfaI CTAG 3 cut(s) 87, 444, 1011
BfmI CTRYAG 1 cut(s) 798
BglII AGATCT 1 cut(s) 823
BisI GCNGC 3 cut(s) 11, 1007, 1178
BlnI CCTAGG 1 cut(s) 443
BlsI GCNGC 3 cut(s) 12, 1008, 1179
BmcAI AGTACT 1 cut(s) 136
BmgT120I GGNCC 1 cut(s) 34
BmsI GCATC 3 cut(s) 388, 574, 1012
Bpu14I TTCGAA 1 cut(s) 143
BpuEI CTTGAG 4 cut(s) 95, 853, 859, 940
BsaAI YACGTR 1 cut(s) 689
BsaI GGTCTC 1 cut(s) 929
BsaJI CCNNGG 3 cut(s) 186, 351, 443
BsaWI WCCGGW 1 cut(s) 730
BsaXI ACNNNNNCTCC 2 cut(s) 1105, 1135
Bsc4I CCNNNNNNNGG 2 cut(s) 192, 947
Bse1I ACTGG 3 cut(s) 374, 788, 788
Bse3DI GCAATG 1 cut(s) 1159
BseDI CCNNGG 3 cut(s) 186, 351, 443
BseGI GGATG 1 cut(s) 403
BseLI CCNNNNNNNGG 2 cut(s) 192, 947
BseMI GCAATG 1 cut(s) 1159
BseMII CTCAG 4 cut(s) 5, 423, 734, 1112
BseNI ACTGG 3 cut(s) 374, 788, 788
BseXI GCAGC 1 cut(s) 1164
BsgI GTGCAG 1 cut(s) 768
Bsh1236I CGCG 1 cut(s) 1170
BshFI GGCC 3 cut(s) 36, 523, 787
BsiHKAI GWGCWC 2 cut(s) 875, 1099
BsiSI CCGG 1 cut(s) 731
BslFI GGGAC 1 cut(s) 228
BslI CCNNNNNNNGG 2 cut(s) 192, 947
BsmAI GTCTC 3 cut(s) 194, 929, 1164
BsmFI GGGAC 1 cut(s) 228
BsnI GGCC 3 cut(s) 36, 523, 787
Bso31I GGTCTC 1 cut(s) 929
Bsp119I TTCGAA 1 cut(s) 143
Bsp1286I GDGCHC 2 cut(s) 875, 1099
Bsp143I GATC 7 cut(s) 295, 564, 802, 823, 937, 995, 1165
Bsp19I CCATGG 1 cut(s) 186
BspACI CCGC 2 cut(s) 693, 1007
BspANI GGCC 3 cut(s) 36, 523, 787
BspCNI CTCAG 4 cut(s) 6, 422, 735, 1111
BspFNI CGCG 1 cut(s) 1170
BspHI TCATGA 2 cut(s) 298, 1162
BspPI GGATC 2 cut(s) 797, 932
BspT104I TTCGAA 1 cut(s) 143
BspTNI GGTCTC 1 cut(s) 929
BsrDI GCAATG 1 cut(s) 1159
BsrI ACTGG 3 cut(s) 374, 788, 788
BssECI CCNNGG 3 cut(s) 186, 351, 443
BssMI GATC 7 cut(s) 295, 564, 802, 823, 937, 995, 1165
BssT1I CCWWGG 2 cut(s) 186, 443
Bst4CI ACNGT 1 cut(s) 1146
Bst6I CTCTTC 1 cut(s) 476
BstBAI YACGTR 1 cut(s) 689
BstBI TTCGAA 1 cut(s) 143
BstC8I GCNNGC 2 cut(s) 596, 697
BstDEI CTNAG 5 cut(s) 14, 409, 639, 743, 1098
BstDSI CCRYGG 1 cut(s) 186
BstF5I GGATG 1 cut(s) 403
BstFNI CGCG 1 cut(s) 1170
BstKTI GATC 7 cut(s) 298, 567, 805, 826, 940, 998, 1168
BstMAI GTCTC 3 cut(s) 194, 929, 1164
BstMBI GATC 7 cut(s) 295, 564, 802, 823, 937, 995, 1165
BstMWI GCNNNNNNNGC 2 cut(s) 718, 1186
BstSFI CTRYAG 1 cut(s) 798
BstUI CGCG 1 cut(s) 1170
BstV1I GCAGC 1 cut(s) 1164
BstX2I RGATCY 2 cut(s) 802, 823
BstXI CCANNNNNNTGG 1 cut(s) 187
BstYI RGATCY 2 cut(s) 802, 823
BsuRI GGCC 3 cut(s) 36, 523, 787
BtgI CCRYGG 1 cut(s) 186
BtsCI GGATG 1 cut(s) 403
BtsI GCAGTG 1 cut(s) 845
BtsIMutI CAGTG 1 cut(s) 845
Cac8I GCNNGC 2 cut(s) 596, 697
CciI TCATGA 2 cut(s) 298, 1162
Cfr13I GGNCC 1 cut(s) 34
Csp6I GTAC 3 cut(s) 135, 345, 690
CviAII CATG 4 cut(s) 187, 299, 847, 1163
CviQI GTAC 3 cut(s) 135, 345, 690
DdeI CTNAG 5 cut(s) 14, 409, 639, 743, 1098
DpnI GATC 7 cut(s) 297, 566, 804, 825, 939, 997, 1167
DpnII GATC 7 cut(s) 295, 564, 802, 823, 937, 995, 1165
DraI TTTAAA 1 cut(s) 172
DrdI GACNNNNNNGTC 1 cut(s) 682
DseDI GACNNNNNNGTC 1 cut(s) 682
EaeI YGGCCR 1 cut(s) 785
Eam1104I CTCTTC 1 cut(s) 476
EarI CTCTTC 1 cut(s) 476
Ecl136II GAGCTC 2 cut(s) 873, 1097
Eco130I CCWWGG 2 cut(s) 186, 443
Eco24I GRGCYC 2 cut(s) 875, 1099
Eco31I GGTCTC 1 cut(s) 929
Eco53kI GAGCTC 2 cut(s) 873, 1097
Eco57I CTGAAG 3 cut(s) 62, 80, 621
EcoICRI GAGCTC 2 cut(s) 873, 1097
EcoT14I CCWWGG 2 cut(s) 186, 443
EcoT38I GRGCYC 2 cut(s) 875, 1099
ErhI CCWWGG 2 cut(s) 186, 443
FaeI CATG 4 cut(s) 190, 302, 850, 1166
FaqI GGGAC 1 cut(s) 228
FatI CATG 4 cut(s) 186, 298, 846, 1162
FbaI TGATCA 1 cut(s) 295
Fnu4HI GCNGC 3 cut(s) 11, 1007, 1178
FokI GGATG 1 cut(s) 410
FriOI GRGCYC 2 cut(s) 875, 1099
Fsp4HI GCNGC 3 cut(s) 11, 1007, 1178
FspBI CTAG 3 cut(s) 87, 444, 1011
GluI GCNGC 3 cut(s) 11, 1007, 1178
HaeIII GGCC 3 cut(s) 36, 523, 787
HapII CCGG 1 cut(s) 731
Hin1II CATG 4 cut(s) 190, 302, 850, 1166
HincII GTYRAC 2 cut(s) 645, 669
HindII GTYRAC 2 cut(s) 645, 669
HinfI GANTC 2 cut(s) 242, 1157
HpaII CCGG 1 cut(s) 731
HphI GGTGA 4 cut(s) 506, 664, 980, 1043
Hpy166II GTNNAC 2 cut(s) 645, 669
Hpy188I TCNGA 4 cut(s) 99, 352, 744, 1101
Hpy188III TCNNGA 4 cut(s) 299, 319, 993, 1163
Hpy8I GTNNAC 2 cut(s) 645, 669
HpyAV CCTTC 6 cut(s) 118, 385, 553, 655, 996, 1085
HpyCH4III ACNGT 1 cut(s) 1146
HpyCH4IV ACGT 2 cut(s) 688, 895
HpyCH4V TGCA 4 cut(s) 209, 749, 770, 1195
HpyF10VI GCNNNNNNNGC 2 cut(s) 718, 1186
HpyF3I CTNAG 5 cut(s) 14, 409, 639, 743, 1098
HpySE526I ACGT 2 cut(s) 688, 895
Hsp92II CATG 4 cut(s) 190, 302, 850, 1166
Ksp22I TGATCA 1 cut(s) 295
Kzo9I GATC 7 cut(s) 295, 564, 802, 823, 937, 995, 1165
LmnI GCTCC 2 cut(s) 1094, 1113
LpnPI CCDG 9 cut(s) 65, 144, 387, 612, 713, 744, 769, 801, 898
Lsp1109I GCAGC 1 cut(s) 1164
LweI GCATC 3 cut(s) 388, 574, 1012
MaeI CTAG 3 cut(s) 87, 444, 1011
MaeII ACGT 2 cut(s) 688, 895
MaeIII GTNAC 1 cut(s) 684
MalI GATC 7 cut(s) 297, 566, 804, 825, 939, 997, 1167
MboI GATC 7 cut(s) 295, 564, 802, 823, 937, 995, 1165
MflI RGATCY 2 cut(s) 802, 823
MhlI GDGCHC 2 cut(s) 875, 1099
MlsI TGGCCA 1 cut(s) 787
MluCI AATT 9 cut(s) 42, 221, 286, 314, 367, 386, 433, 542, 625
MluNI TGGCCA 1 cut(s) 787
MlyI GAGTC 1 cut(s) 1166
MmeI TCCRAC 1 cut(s) 878
MnlI CCTC 9 cut(s) 25, 60, 243, 346, 358, 513, 540, 628, 828
Mox20I TGGCCA 1 cut(s) 787
MscI TGGCCA 1 cut(s) 787
MseI TTAA 2 cut(s) 171, 474
MslI CAYNNNNRTG 1 cut(s) 185
Msp20I TGGCCA 1 cut(s) 787
MspI CCGG 1 cut(s) 731
MvnI CGCG 1 cut(s) 1170
MwoI GCNNNNNNNGC 2 cut(s) 718, 1186
NcoI CCATGG 1 cut(s) 186
NdeII GATC 7 cut(s) 295, 564, 802, 823, 937, 995, 1165
NlaIII CATG 4 cut(s) 190, 302, 850, 1166
NmuCI GTSAC 1 cut(s) 684
NspV TTCGAA 1 cut(s) 143
PagI TCATGA 2 cut(s) 298, 1162
PcsI WCGNNNNNNNCGW 1 cut(s) 866
PfeI GAWTC 1 cut(s) 242
PflMI CCANNNNNTGG 1 cut(s) 947
PkrI GCNGC 3 cut(s) 12, 1008, 1179
PleI GAGTC 1 cut(s) 1165
PpsI GAGTC 1 cut(s) 1165
Ppu21I YACGTR 1 cut(s) 689
Psp124BI GAGCTC 2 cut(s) 875, 1099
PspPI GGNCC 1 cut(s) 34
PsuI RGATCY 2 cut(s) 802, 823
RsaI GTAC 3 cut(s) 136, 346, 691
RsaNI GTAC 3 cut(s) 135, 345, 690
RseI CAYNNNNRTG 1 cut(s) 185
SacI GAGCTC 2 cut(s) 875, 1099
SaqAI TTAA 2 cut(s) 171, 474
SatI GCNGC 3 cut(s) 11, 1007, 1178
Sau3AI GATC 7 cut(s) 295, 564, 802, 823, 937, 995, 1165
Sau96I GGNCC 1 cut(s) 34
ScaI AGTACT 1 cut(s) 136
SchI GAGTC 1 cut(s) 1166
SduI GDGCHC 2 cut(s) 875, 1099
SfaNI GCATC 3 cut(s) 388, 574, 1012
SfcI CTRYAG 1 cut(s) 798
SfuI TTCGAA 1 cut(s) 143
SmiMI CAYNNNNRTG 1 cut(s) 185
SmlI CTYRAG 4 cut(s) 110, 832, 874, 955
SmoI CTYRAG 4 cut(s) 110, 832, 874, 955
Sse9I AATT 9 cut(s) 42, 221, 286, 314, 367, 386, 433, 542, 625
SsiI CCGC 2 cut(s) 693, 1007
SspMI CTAG 3 cut(s) 87, 444, 1011
SstI GAGCTC 2 cut(s) 875, 1099
StyI CCWWGG 2 cut(s) 186, 443
TaaI ACNGT 1 cut(s) 1146
TaiI ACGT 2 cut(s) 691, 898
TaqI TCGA 2 cut(s) 46, 143
TasI AATT 9 cut(s) 42, 221, 286, 314, 367, 386, 433, 542, 625
TatI WGTACW 1 cut(s) 134
TauI GCSGC 1 cut(s) 1009
TfiI GAWTC 1 cut(s) 242
Tru1I TTAA 2 cut(s) 171, 474
Tru9I TTAA 2 cut(s) 171, 474
TscAI CASTG 1 cut(s) 845
TseFI GTSAC 1 cut(s) 684
TseI GCWGC 2 cut(s) 10, 1177
Tsp45I GTSAC 1 cut(s) 684
TspDTI ATGAA 3 cut(s) 242, 315, 731
TspRI CASTG 1 cut(s) 845
Van91I CCANNNNNTGG 1 cut(s) 947
XapI RAATTY 4 cut(s) 221, 367, 433, 625
XmaJI CCTAGG 1 cut(s) 443
XspI CTAG 3 cut(s) 87, 444, 1011
ZrmI AGTACT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.