RLG00000026262

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000766
Physical Location & Seq
Forward (+)
1733158 .. 1734477
1320 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026262

Sequence Viewer

Length: 1320 bp
ATGTTTGTCACTAGAGAATACCCGCAGGTTTCATATATTCAACGTAATTCTTCAATTCTTCAATCTCCGACGTCGAAAAACACCAGAAAAGAAGACGAGATGGTGCAGCTGCTGAGTATGAAAGATTTTGAGGGCAAAGCAATTCGACCTTGTTCGTCCTACATTGATATTTTTCTTCAAGTTGCTAGTGTTCTTCAGAATAAGAAACTAAAGCAAGGTATGGAGAAAAGTACTCATTTGAAAACCAAGATGGCCGGTATAGGGTTTAAAGAACCCATTTCCATGGTAGGGAAGGAGACTTATGCAAAGGGACAAAAATTTGTGAACTCTGTTTTTGAATCTGTGATCATGAAAGGGTCAAAAAGAAAGGGTTTTGACAATTTTGATGATCATGAAAGGGTTTTTCAATTCAAGAAGCAGAAGATGGATATGGGTGGTACTAGTACCTCCGAGGCTAAAGAACCAAATTCGCCAGTCGTAAAAGAATTGAAGGTGGATGCGTTATACTCAGTTGGCACAATAAAGACAGAAAATTCTATGCCTAGGGCAAACTGTATAATGAGTTGTGATGTTAAGAAGAAGAGTACTAATGGTGATGATGATAACCAATGGGGTTTGAGGCCGAAGCCGAAGAATATCAAATTGAGGAAGCCAAAGAAGGATATCACAACACAAGGTATTGATGCTAGCGGCTTGCCTGAAGAATACAAGAGAAAGATTGAAAATTTTGATAAGGCTAAGGCGACTTTGGTGATACAGAAGGAATTGACCAAGACTGATTTGTCACGTACCGCTTGCCGGTTGTCAATGCCTATGAACCAAATCAAACCGGAAAGTTTTCTGAGTGCAGATGAGATAAAGTTTCTTGCAAAACAAAATGACTGGCCAGTTTCGGTTATAGATCCCCAACTTGAAGAAGAAGATCTAACCTTGAGGCAGTGGAACATGGAAAAGAACACGGGTAAGACGAGCTCAAGCTATGTGTTGAAAACACGTTGGAATGAAGTTGCCGATAAAAACAATCTCAAACAAAAAGATGTAGTCCAAGTTTGGTCATTCCGGGATGTGGGAAACAACCTTCACTTTGCTATTGTTTTGGTTAAAAAGGGTGGTGAGCATAGTAGTAGTCAAGGGTATAGCCAGCCTTCTTCGCCTTCAACAACACCGCGTGATATTTTTGATGGTGAAAGAAGAAGTGGTAGTGGTCAAGGAAGCGAATCAAAGGAAGAAGGGATGATAGGAGTTAATAGCAATGCTGAGAGTAATAGTGAGAGAGAAGTACTACGAGCAATGGCTCTAAGGAAAACCATGAAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

440

Amino Acids

49.62

Weight (kDa)

9.43

Isoelectric Point (pI)

44.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 246 - 338 4.5e-16 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 781
AatII GACGTC 1 cut(s) 74
Acc36I ACCTGC 1 cut(s) 16
AccII CGCG 1 cut(s) 1168
AciI CCGC 4 cut(s) 23, 690, 792, 1166
AclWI GGATC 1 cut(s) 896
AcoI YGGCCR 2 cut(s) 252, 884
AcsI RAATTY 5 cut(s) 317, 466, 532, 724, 1314
AcuI CTGAAG 2 cut(s) 179, 720
AcyI GRCGYC 1 cut(s) 71
AdeI CACNNNGTG 1 cut(s) 1169
AfaI GTAC 6 cut(s) 232, 439, 445, 586, 790, 1281
AfiI CCNNNNNNNGG 4 cut(s) 261, 288, 798, 1066
AflIII ACRYGT 1 cut(s) 992
AhlI ACTAGT 1 cut(s) 440
AluBI AGCT 3 cut(s) 109, 972, 978
AluI AGCT 3 cut(s) 109, 972, 978
Alw21I GWGCWC 1 cut(s) 974
Alw26I GTCTC 1 cut(s) 290
AlwI GGATC 1 cut(s) 896
AlwNI CAGNNNCTG 1 cut(s) 112
AoxI GGCC 3 cut(s) 252, 620, 884
ApeKI GCWGC 2 cut(s) 106, 109
ApoI RAATTY 5 cut(s) 317, 466, 532, 724, 1314
Asp700I GAANNNNTTC 1 cut(s) 837
AspA2I CCTAGG 1 cut(s) 542
AsuC2I CCSGG 1 cut(s) 1061
AsuHPI GGTGA 4 cut(s) 605, 763, 1124, 1196
AsuNHI GCTAGC 1 cut(s) 686
AvrII CCTAGG 1 cut(s) 542
BalI TGGCCA 1 cut(s) 886
BanII GRGCYC 1 cut(s) 974
BbsI GAAGAC 1 cut(s) 99
Bbv12I GWGCWC 1 cut(s) 974
BbvI GCAGC 2 cut(s) 96, 118
BccI CCATC 4 cut(s) 94, 244, 418, 1175
BclI TGATCA 2 cut(s) 345, 388
BcnI CCSGG 1 cut(s) 1061
BcoDI GTCTC 1 cut(s) 290
BcuI ACTAGT 1 cut(s) 440
BfaI CTAG 5 cut(s) 12, 186, 441, 543, 687
BfuAI ACCTGC 1 cut(s) 16
BglII AGATCT 1 cut(s) 922
BisI GCNGC 3 cut(s) 107, 110, 691
BlnI CCTAGG 1 cut(s) 542
BlsI GCNGC 3 cut(s) 108, 111, 692
BmcAI AGTACT 3 cut(s) 232, 586, 1281
Bme1390I CCNGG 1 cut(s) 1061
BmrFI CCNGG 1 cut(s) 1061
BmsI GCATC 2 cut(s) 487, 673
BmtI GCTAGC 1 cut(s) 690
BpiI GAAGAC 1 cut(s) 99
Bpu10I CCTNAGC 1 cut(s) 738
BpuEI CTTGAG 2 cut(s) 952, 958
BpuMI CCSGG 1 cut(s) 1061
BsaAI YACGTR 1 cut(s) 788
BsaHI GRCGYC 1 cut(s) 71
BsaJI CCNNGG 3 cut(s) 282, 450, 542
BsaWI WCCGGW 1 cut(s) 829
Bsc4I CCNNNNNNNGG 4 cut(s) 261, 288, 798, 1066
Bse118I RCCGGY 2 cut(s) 254, 798
Bse1I ACTGG 3 cut(s) 473, 887, 887
Bse3DI GCAATG 2 cut(s) 1258, 1296
BseDI CCNNGG 3 cut(s) 282, 450, 542
BseGI GGATG 3 cut(s) 502, 1069, 1239
BseLI CCNNNNNNNGG 4 cut(s) 261, 288, 798, 1066
BseMI GCAATG 2 cut(s) 1258, 1296
BseMII CTCAG 4 cut(s) 104, 522, 833, 1248
BseNI ACTGG 3 cut(s) 473, 887, 887
BseXI GCAGC 2 cut(s) 96, 118
BsgI GTGCAG 2 cut(s) 125, 867
Bsh1236I CGCG 1 cut(s) 1168
BshFI GGCC 3 cut(s) 254, 622, 886
BsiHKAI GWGCWC 1 cut(s) 974
BsiSI CCGG 4 cut(s) 255, 799, 830, 1060
BslFI GGGAC 1 cut(s) 324
BslI CCNNNNNNNGG 4 cut(s) 261, 288, 798, 1066
BsmAI GTCTC 1 cut(s) 290
BsmFI GGGAC 1 cut(s) 324
BsnI GGCC 3 cut(s) 254, 622, 886
Bsp1286I GDGCHC 1 cut(s) 974
Bsp143I GATC 4 cut(s) 345, 388, 901, 922
Bsp19I CCATGG 1 cut(s) 282
BspACI CCGC 4 cut(s) 23, 690, 792, 1166
BspANI GGCC 3 cut(s) 254, 622, 886
BspCNI CTCAG 4 cut(s) 105, 521, 834, 1249
BspFNI CGCG 1 cut(s) 1168
BspHI TCATGA 2 cut(s) 348, 391
BspMI ACCTGC 1 cut(s) 16
BspOI GCTAGC 1 cut(s) 690
BspPI GGATC 1 cut(s) 896
BsrDI GCAATG 2 cut(s) 1258, 1296
BsrFI RCCGGY 2 cut(s) 254, 798
BsrI ACTGG 3 cut(s) 473, 887, 887
BssAI RCCGGY 2 cut(s) 254, 798
BssECI CCNNGG 3 cut(s) 282, 450, 542
BssMI GATC 4 cut(s) 345, 388, 901, 922
BssNI GRCGYC 1 cut(s) 71
BssT1I CCWWGG 2 cut(s) 282, 542
Bst4CI ACNGT 1 cut(s) 554
Bst6I CTCTTC 1 cut(s) 575
BstACI GRCGYC 1 cut(s) 71
BstBAI YACGTR 1 cut(s) 788
BstC8I GCNNGC 4 cut(s) 688, 695, 796, 1142
BstDEI CTNAG 6 cut(s) 113, 508, 738, 842, 1257, 1298
BstDSI CCRYGG 1 cut(s) 282
BstF5I GGATG 3 cut(s) 502, 1069, 1239
BstFNI CGCG 1 cut(s) 1168
BstKTI GATC 4 cut(s) 348, 391, 904, 925
BstMAI GTCTC 1 cut(s) 290
BstMBI GATC 4 cut(s) 345, 388, 901, 922
BstMWI GCNNNNNNNGC 1 cut(s) 1150
BstSCI CCNGG 1 cut(s) 1059
BstUI CGCG 1 cut(s) 1168
BstV1I GCAGC 2 cut(s) 96, 118
BstV2I GAAGAC 1 cut(s) 99
BstX2I RGATCY 2 cut(s) 901, 922
BstXI CCANNNNNNTGG 1 cut(s) 283
BstYI RGATCY 2 cut(s) 901, 922
BsuRI GGCC 3 cut(s) 254, 622, 886
BtgI CCRYGG 1 cut(s) 282
BtsCI GGATG 3 cut(s) 502, 1069, 1239
BtsI GCAGTG 1 cut(s) 944
BtsIMutI CAGTG 1 cut(s) 944
BveI ACCTGC 1 cut(s) 16
Cac8I GCNNGC 4 cut(s) 688, 695, 796, 1142
CaiI CAGNNNCTG 1 cut(s) 112
CciI TCATGA 2 cut(s) 348, 391
Cfr10I RCCGGY 2 cut(s) 254, 798
Csp6I GTAC 6 cut(s) 231, 438, 444, 585, 789, 1280
CviAII CATG 5 cut(s) 283, 349, 392, 946, 1309
CviQI GTAC 6 cut(s) 231, 438, 444, 585, 789, 1280
DdeI CTNAG 6 cut(s) 113, 508, 738, 842, 1257, 1298
DpnI GATC 4 cut(s) 347, 390, 903, 924
DpnII GATC 4 cut(s) 345, 388, 901, 922
DraI TTTAAA 1 cut(s) 268
DraIII CACNNNGTG 1 cut(s) 1169
DrdI GACNNNNNNGTC 1 cut(s) 781
DseDI GACNNNNNNGTC 1 cut(s) 781
EaeI YGGCCR 2 cut(s) 252, 884
Eam1104I CTCTTC 1 cut(s) 575
EarI CTCTTC 1 cut(s) 575
Ecl136II GAGCTC 1 cut(s) 972
Eco130I CCWWGG 2 cut(s) 282, 542
Eco24I GRGCYC 1 cut(s) 974
Eco32I GATATC 1 cut(s) 664
Eco53kI GAGCTC 1 cut(s) 972
Eco57I CTGAAG 2 cut(s) 179, 720
EcoICRI GAGCTC 1 cut(s) 972
EcoRV GATATC 1 cut(s) 664
EcoT14I CCWWGG 2 cut(s) 282, 542
EcoT38I GRGCYC 1 cut(s) 974
ErhI CCWWGG 2 cut(s) 282, 542
FaeI CATG 5 cut(s) 286, 352, 395, 949, 1312
FaqI GGGAC 1 cut(s) 324
FatI CATG 5 cut(s) 282, 348, 391, 945, 1308
FauI CCCGC 1 cut(s) 30
FbaI TGATCA 2 cut(s) 345, 388
Fnu4HI GCNGC 3 cut(s) 107, 110, 691
FokI GGATG 3 cut(s) 509, 1076, 1246
FriOI GRGCYC 1 cut(s) 974
Fsp4HI GCNGC 3 cut(s) 107, 110, 691
FspBI CTAG 5 cut(s) 12, 186, 441, 543, 687
GluI GCNGC 3 cut(s) 107, 110, 691
HaeIII GGCC 3 cut(s) 254, 622, 886
HapII CCGG 4 cut(s) 255, 799, 830, 1060
Hin1I GRCGYC 1 cut(s) 71
Hin1II CATG 5 cut(s) 286, 352, 395, 949, 1312
HinfI GANTC 2 cut(s) 338, 1217
HpaII CCGG 4 cut(s) 255, 799, 830, 1060
HphI GGTGA 4 cut(s) 605, 763, 1124, 1196
Hpy166II GTNNAC 1 cut(s) 325
Hpy188I TCNGA 4 cut(s) 69, 198, 451, 843
Hpy188III TCNNGA 3 cut(s) 349, 392, 412
Hpy8I GTNNAC 1 cut(s) 325
Hpy99I CGWCG 2 cut(s) 73, 76
HpyAV CCTTC 8 cut(s) 286, 484, 652, 754, 1088, 1155, 1164, 1223
HpyCH4III ACNGT 1 cut(s) 554
HpyCH4IV ACGT 4 cut(s) 43, 71, 787, 994
HpyCH4V TGCA 4 cut(s) 106, 305, 848, 869
HpyF10VI GCNNNNNNNGC 1 cut(s) 1150
HpyF3I CTNAG 6 cut(s) 113, 508, 738, 842, 1257, 1298
HpySE526I ACGT 4 cut(s) 43, 71, 787, 994
Hsp92I GRCGYC 1 cut(s) 71
Hsp92II CATG 5 cut(s) 286, 352, 395, 949, 1312
Ksp22I TGATCA 2 cut(s) 345, 388
Kzo9I GATC 4 cut(s) 345, 388, 901, 922
Lsp1109I GCAGC 2 cut(s) 96, 118
LweI GCATC 2 cut(s) 487, 673
MaeI CTAG 5 cut(s) 12, 186, 441, 543, 687
MaeII ACGT 4 cut(s) 43, 71, 787, 994
MaeIII GTNAC 2 cut(s) 7, 783
MalI GATC 4 cut(s) 347, 390, 903, 924
MboI GATC 4 cut(s) 345, 388, 901, 922
MflI RGATCY 2 cut(s) 901, 922
MhlI GDGCHC 1 cut(s) 974
MlsI TGGCCA 1 cut(s) 886
MluNI TGGCCA 1 cut(s) 886
MmeI TCCRAC 2 cut(s) 92, 977
MnlI CCTC 6 cut(s) 124, 445, 457, 612, 639, 927
Mox20I TGGCCA 1 cut(s) 886
MroXI GAANNNNTTC 1 cut(s) 837
MscI TGGCCA 1 cut(s) 886
MseI TTAA 5 cut(s) 267, 573, 1101, 1245, 1318
MslI CAYNNNNRTG 1 cut(s) 281
Msp20I TGGCCA 1 cut(s) 886
MspA1I CMGCKG 1 cut(s) 109
MspI CCGG 4 cut(s) 255, 799, 830, 1060
MspR9I CCNGG 1 cut(s) 1061
MvnI CGCG 1 cut(s) 1168
MwoI GCNNNNNNNGC 1 cut(s) 1150
NciI CCSGG 1 cut(s) 1061
NcoI CCATGG 1 cut(s) 282
NdeII GATC 4 cut(s) 345, 388, 901, 922
NheI GCTAGC 1 cut(s) 686
NlaIII CATG 5 cut(s) 286, 352, 395, 949, 1312
NmuCI GTSAC 2 cut(s) 7, 783
PagI TCATGA 2 cut(s) 348, 391
PcsI WCGNNNNNNNCGW 1 cut(s) 965
PdmI GAANNNNTTC 1 cut(s) 837
PfeI GAWTC 2 cut(s) 338, 1217
PfoI TCCNGGA 1 cut(s) 1059
PkrI GCNGC 3 cut(s) 108, 111, 692
Ppu21I YACGTR 1 cut(s) 788
Psp124BI GAGCTC 1 cut(s) 974
PstNI CAGNNNCTG 1 cut(s) 112
PsuI RGATCY 2 cut(s) 901, 922
PvuII CAGCTG 1 cut(s) 109
RsaI GTAC 6 cut(s) 232, 439, 445, 586, 790, 1281
RsaNI GTAC 6 cut(s) 231, 438, 444, 585, 789, 1280
RseI CAYNNNNRTG 1 cut(s) 281
SacI GAGCTC 1 cut(s) 974
SaqAI TTAA 5 cut(s) 267, 573, 1101, 1245, 1318
SatI GCNGC 3 cut(s) 107, 110, 691
Sau3AI GATC 4 cut(s) 345, 388, 901, 922
ScaI AGTACT 3 cut(s) 232, 586, 1281
ScrFI CCNGG 1 cut(s) 1061
SduI GDGCHC 1 cut(s) 974
SfaNI GCATC 2 cut(s) 487, 673
SmiMI CAYNNNNRTG 1 cut(s) 281
SmlI CTYRAG 2 cut(s) 931, 973
SmoI CTYRAG 2 cut(s) 931, 973
SpeI ACTAGT 1 cut(s) 440
SsiI CCGC 4 cut(s) 23, 690, 792, 1166
SspMI CTAG 5 cut(s) 12, 186, 441, 543, 687
SstI GAGCTC 1 cut(s) 974
StyD4I CCNGG 1 cut(s) 1059
StyI CCWWGG 2 cut(s) 282, 542
TaaI ACNGT 1 cut(s) 554
TaiI ACGT 4 cut(s) 46, 74, 790, 997
TaqI TCGA 2 cut(s) 74, 145
TatI WGTACW 3 cut(s) 230, 584, 1279
TauI GCSGC 1 cut(s) 693
TfiI GAWTC 2 cut(s) 338, 1217
Tru1I TTAA 5 cut(s) 267, 573, 1101, 1245, 1318
Tru9I TTAA 5 cut(s) 267, 573, 1101, 1245, 1318
TscAI CASTG 1 cut(s) 944
TseFI GTSAC 2 cut(s) 7, 783
TseI GCWGC 2 cut(s) 106, 109
Tsp45I GTSAC 2 cut(s) 7, 783
TspDTI ATGAA 6 cut(s) 21, 134, 365, 408, 830, 1017
TspRI CASTG 1 cut(s) 944
XapI RAATTY 5 cut(s) 317, 466, 532, 724, 1314
XmaJI CCTAGG 1 cut(s) 542
XmnI GAANNNNTTC 1 cut(s) 837
XspI CTAG 5 cut(s) 12, 186, 441, 543, 687
ZraI GACGTC 1 cut(s) 72
ZrmI AGTACT 3 cut(s) 232, 586, 1281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.