FvH4_6g01440

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000766
Physical Location & Seq
Reverse (-)
812200 .. 812775
576 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g01440.t1

Sequence Viewer

Length: 405 bp
ATGGCCAATCCTGCAGAAGTGTACAACATGATCCAACTCTTGCTGCAGAATGCCAAATTGGTGACGCAGAAGAAGCTTACTTGTAGCGATGTGGCGAGAACTCACAACCGTCTATTAGTACGAGTGAATCAAATAAAACTTGAAGTGTTTCTTAATGAGGAAGAACTGGGCGTTATCGGTGCACAAGAAAGCGTCCCGGTGCGAGTGATAGGACCAGGCTCAGTTGAGTCGAATTTACAGTTTAAGAAGTATAATCTTTCTGAGACAGCCAAGTTCTATGCTATAACACACGGTTGGGGAGGATTTGTGCGACGGACTGGTCTTCAGGTAATGGATACAATTCAGCTGTGGTCATTCCGGGTTAACGGCAATCTATGCTTCGCAGTTGCTAAAGTAGAGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

135

Amino Acids

15.06

Weight (kDa)

9.26

Isoelectric Point (pI)

33.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 16 - 103 1.9e-11 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 318
AclWI GGATC 1 cut(s) 25
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 232
AcuI CTGAAG 1 cut(s) 308
AfaI GTAC 2 cut(s) 23, 120
AgsI TTSAA 1 cut(s) 143
AjnI CCWGG 1 cut(s) 214
AjuI GAANNNNNNNTTGG 2 cut(s) 41, 73
AluBI AGCT 3 cut(s) 76, 346, 400
AluI AGCT 3 cut(s) 76, 346, 400
Alw21I GWGCWC 1 cut(s) 184
Alw26I GTCTC 1 cut(s) 257
Alw44I GTGCAC 1 cut(s) 180
AlwI GGATC 1 cut(s) 25
AoxI GGCC 1 cut(s) 3
ApaLI GTGCAC 1 cut(s) 180
ApeKI GCWGC 1 cut(s) 43
ApoI RAATTY 1 cut(s) 232
Asp700I GAANNNNTTC 1 cut(s) 147
AspS9I GGNCC 1 cut(s) 212
AsuC2I CCSGG 2 cut(s) 197, 359
AsuHPI GGTGA 1 cut(s) 73
AvaII GGWCC 1 cut(s) 212
BaeGI GKGCMC 1 cut(s) 184
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 314
Bbv12I GWGCWC 1 cut(s) 184
BbvI GCAGC 1 cut(s) 30
BceAI ACGGC 1 cut(s) 382
BciT130I CCWGG 1 cut(s) 216
BciVI GTATCC 1 cut(s) 328
BcnI CCSGG 2 cut(s) 197, 359
BcoDI GTCTC 1 cut(s) 257
BfmI CTRYAG 2 cut(s) 12, 44
BfuI GTATCC 1 cut(s) 328
BisI GCNGC 1 cut(s) 44
BlsI GCNGC 1 cut(s) 45
Bme1390I CCNGG 3 cut(s) 197, 216, 359
Bme18I GGWCC 1 cut(s) 212
BmgT120I GGNCC 1 cut(s) 212
BmrFI CCNGG 3 cut(s) 197, 216, 359
BmrI ACTGGG 1 cut(s) 176
BmuI ACTGGG 1 cut(s) 176
BpiI GAAGAC 1 cut(s) 314
BpuMI CCSGG 2 cut(s) 197, 359
BsaXI ACNNNNNCTCC 2 cut(s) 291, 321
Bse1I ACTGG 2 cut(s) 171, 322
BseBI CCWGG 1 cut(s) 216
BseMII CTCAG 2 cut(s) 234, 252
BseNI ACTGG 2 cut(s) 171, 322
BseSI GKGCMC 1 cut(s) 184
BseXI GCAGC 1 cut(s) 30
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 184
BsiSI CCGG 2 cut(s) 197, 358
BslFI GGGAC 1 cut(s) 179
BsmAI GTCTC 1 cut(s) 257
BsmFI GGGAC 1 cut(s) 179
BsmI GAATGC 1 cut(s) 55
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 184
Bsp1407I TGTACA 1 cut(s) 21
Bsp143I GATC 1 cut(s) 30
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 233, 253
BspMAI CTGCAG 2 cut(s) 16, 48
BspPI GGATC 1 cut(s) 25
BsrGI TGTACA 1 cut(s) 21
BsrI ACTGG 2 cut(s) 171, 322
BssMI GATC 1 cut(s) 30
Bst2UI CCWGG 1 cut(s) 216
Bst4CI ACNGT 3 cut(s) 110, 240, 293
BstAPI GCANNNNNTGC 1 cut(s) 375
BstAUI TGTACA 1 cut(s) 21
BstDEI CTNAG 2 cut(s) 220, 261
BstKTI GATC 1 cut(s) 33
BstMAI GTCTC 1 cut(s) 257
BstMBI GATC 1 cut(s) 30
BstMWI GCNNNNNNNGC 3 cut(s) 11, 73, 375
BstNI CCWGG 1 cut(s) 216
BstSCI CCNGG 3 cut(s) 195, 214, 357
BstSFI CTRYAG 2 cut(s) 12, 44
BstSLI GKGCMC 1 cut(s) 184
BstV1I GCAGC 1 cut(s) 30
BstV2I GAAGAC 1 cut(s) 314
BsuI GTATCC 1 cut(s) 328
BsuRI GGCC 1 cut(s) 5
BtgZI GCGATG 1 cut(s) 102
Cfr13I GGNCC 1 cut(s) 212
CseI GACGC 2 cut(s) 73, 181
Csp6I GTAC 2 cut(s) 22, 119
CviAII CATG 1 cut(s) 28
CviJI RGCY 6 cut(s) 5, 76, 219, 269, 346, 400
CviKI_1 RGCY 6 cut(s) 5, 76, 219, 269, 346, 400
CviQI GTAC 2 cut(s) 22, 119
DdeI CTNAG 2 cut(s) 220, 261
DpnI GATC 1 cut(s) 32
DpnII GATC 1 cut(s) 30
DrdI GACNNNNNNGTC 1 cut(s) 318
DseDI GACNNNNNNGTC 1 cut(s) 318
EaeI YGGCCR 1 cut(s) 3
Eco47I GGWCC 1 cut(s) 212
Eco57I CTGAAG 1 cut(s) 308
EcoRII CCWGG 1 cut(s) 214
FaeI CATG 1 cut(s) 31
FaiI YATR 6 cut(s) 29, 252, 279, 284, 376, 403
FalI AAGNNNNNCTT 2 cut(s) 135, 167
FaqI GGGAC 1 cut(s) 179
FatI CATG 1 cut(s) 27
Fnu4HI GCNGC 1 cut(s) 44
Fsp4HI GCNGC 1 cut(s) 44
GluI GCNGC 1 cut(s) 44
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 197, 358
HgaI GACGC 2 cut(s) 73, 181
Hin1II CATG 1 cut(s) 31
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HindIII AAGCTT 1 cut(s) 74
HinfI GANTC 2 cut(s) 127, 227
HpaI GTTAAC 1 cut(s) 364
HpaII CCGG 2 cut(s) 197, 358
HphI GGTGA 1 cut(s) 73
Hpy166II GTNNAC 3 cut(s) 22, 182, 364
Hpy188I TCNGA 1 cut(s) 262
Hpy8I GTNNAC 3 cut(s) 22, 182, 364
Hpy99I CGWCG 1 cut(s) 315
HpyCH4III ACNGT 3 cut(s) 110, 240, 293
HpyCH4V TGCA 3 cut(s) 14, 46, 182
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 73, 375
HpyF3I CTNAG 2 cut(s) 220, 261
Hsp92II CATG 1 cut(s) 31
KspAI GTTAAC 1 cut(s) 364
Kzo9I GATC 1 cut(s) 30
LpnPI CCDG 8 cut(s) 24, 152, 201, 210, 228, 303, 311, 371
Lsp1109I GCAGC 1 cut(s) 30
MaeIII GTNAC 1 cut(s) 61
MalI GATC 1 cut(s) 32
MboI GATC 1 cut(s) 30
MboII GAAGA 3 cut(s) 82, 173, 314
MhlI GDGCHC 1 cut(s) 184
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 56, 232, 339
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 236
MmeI TCCRAC 1 cut(s) 58
MnlI CCTC 2 cut(s) 151, 293
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 147
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 153, 243, 363
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 346
MspI CCGG 2 cut(s) 197, 358
MspR9I CCNGG 3 cut(s) 197, 216, 359
Mva1269I GAATGC 1 cut(s) 55
MvaI CCWGG 1 cut(s) 216
MwoI GCNNNNNNNGC 3 cut(s) 11, 73, 375
NciI CCSGG 2 cut(s) 197, 359
NdeII GATC 1 cut(s) 30
NlaIII CATG 1 cut(s) 31
NmuCI GTSAC 1 cut(s) 61
PctI GAATGC 1 cut(s) 55
PdmI GAANNNNTTC 1 cut(s) 147
PfeI GAWTC 1 cut(s) 127
PkrI GCNGC 1 cut(s) 45
PleI GAGTC 1 cut(s) 235
PpsI GAGTC 1 cut(s) 235
Psp6I CCWGG 1 cut(s) 214
PspGI CCWGG 1 cut(s) 214
PspPI GGNCC 1 cut(s) 212
PstI CTGCAG 2 cut(s) 16, 48
PvuII CAGCTG 1 cut(s) 346
RsaI GTAC 2 cut(s) 23, 120
RsaNI GTAC 2 cut(s) 22, 119
SaqAI TTAA 3 cut(s) 153, 243, 363
SatI GCNGC 1 cut(s) 44
Sau3AI GATC 1 cut(s) 30
Sau96I GGNCC 1 cut(s) 212
SchI GAGTC 1 cut(s) 236
ScrFI CCNGG 3 cut(s) 197, 216, 359
SduI GDGCHC 1 cut(s) 184
SetI ASST 4 cut(s) 78, 330, 348, 402
SfcI CTRYAG 2 cut(s) 12, 44
SinI GGWCC 1 cut(s) 212
Sse9I AATT 3 cut(s) 56, 232, 339
StyD4I CCNGG 3 cut(s) 195, 214, 357
TaaI ACNGT 3 cut(s) 110, 240, 293
TaqI TCGA 1 cut(s) 230
TasI AATT 3 cut(s) 56, 232, 339
TatI WGTACW 1 cut(s) 21
TfiI GAWTC 1 cut(s) 127
Tru1I TTAA 3 cut(s) 153, 243, 363
Tru9I TTAA 3 cut(s) 153, 243, 363
TseFI GTSAC 1 cut(s) 61
TseI GCWGC 1 cut(s) 43
Tsp45I GTSAC 1 cut(s) 61
TspGWI ACGGA 1 cut(s) 328
VneI GTGCAC 1 cut(s) 180
VpaK11BI GGWCC 1 cut(s) 212
XapI RAATTY 1 cut(s) 232
XmnI GAANNNNTTC 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.