Rmu_sc0005371.1_g000007

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005371.1
OG0000766
Physical Location & Seq
Forward (+)
31337 .. 32668
1332 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005371.1_g000007.1.cds

Sequence Viewer

Length: 1332 bp
atggcgccgctagtgagtattgaagacttcatagaaaaacaacttcgacctggttcaaccttgaatattttgggtcaggttgcttgtgttgcacgggagaaactcaagcaaaggaggaaggagaaaagtattaaagtcaaaactcagatgggcagtagtagtacccccaaggttaaagagcccaattatgtagctgaaacatattccaaagagacggatgctgatatgaacaaaagaaagagtcgagaggacgatgttgatcatcatgacaagggttttcaattgaaacaacggaagacaatttttttgcacgaggaatttggtactacctccaagactaaagaaccaaattcatcaatgggtaaagaatttgaggaggatgctttatactcagttggcacaataaggagagaaagttctttccttaggacaaattatgtaacgagctctgtgtttggctccaaaactggtccggctagcaaacactctgattcagggtttcaggaaaaaaatgggttgacaaagaagaagagtattgatggtggtgaggatggtaaagcttggagtttgaagcccaagccgaagaatatgaagattaagaaggaaaaggggttgagattcaagaggtctaaggagcatatgagagcacccagatatagtactgatcctaatgacttgcctgaagacttgaaaaggaagattgaaactttggaaggtattgaaagttttgtaaaggctaagatgactatggtgatagagaagcatctgttcgagactgatctgactgatagcgcaagccgattgtcaatgcctctgaaacaaatcaaaccggagagtttcttggacgaggatgagatcaagtttcttgcatctcaacaagactggccggtttcggttataagtcccaaacttaaggacgaaaatctaactttcaggcaatggaacatgaataagaataacggcaacgttagctcaagttatgtgttgaaaacaggttggaacgaacttcacaagaaaaacggtctccaagtaaatgatgtaatccaagtttggtcatttcgagatgtgggaaacaaccttcactttgctcttgttcttgttgagaaggcaacagagggcggtttaagaccatcactagatgggtgtagtctaccttcttcacgatcaacaaagccgcctagcaacaataatgttgatggtgaaaatagtagttgtaatattggccaaggaacagaaataaaagaagaagagatgataagaggagatgaaagtcgtgaggttaatgatgaaagcaatagcaggagtggaagaatttcaacgtacaccgtctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

443

Amino Acids

50.08

Weight (kDa)

9.09

Isoelectric Point (pI)

50.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 899
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 1150
AciI CCGC 3 cut(s) 8, 1119, 1175
AclI AACGTT 1 cut(s) 966
AclWI GGATC 1 cut(s) 659
AcoI YGGCCR 2 cut(s) 884, 1222
AcsI RAATTY 4 cut(s) 317, 349, 368, 1311
AcuI CTGAAG 1 cut(s) 702
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 163, 325, 661, 1322
AflII CTTAAG 1 cut(s) 911
AjnI CCWGG 1 cut(s) 49
AloI GAACNNNNNNTCC 2 cut(s) 400, 432
AluBI AGCT 4 cut(s) 194, 447, 560, 972
AluI AGCT 4 cut(s) 194, 447, 560, 972
Alw21I GWGCWC 2 cut(s) 449, 649
Alw26I GTCTC 3 cut(s) 206, 767, 1028
AlwI GGATC 1 cut(s) 659
AoxI GGCC 2 cut(s) 884, 1222
ApoI RAATTY 4 cut(s) 317, 349, 368, 1311
ArsI GACNNNNNNTTYG 2 cut(s) 289, 321
Asp700I GAANNNNTTC 1 cut(s) 1312
AspLEI GCGC 2 cut(s) 7, 794
AspS9I GGNCC 1 cut(s) 470
AsuHPI GGTGA 3 cut(s) 557, 763, 1211
AsuNHI GCTAGC 1 cut(s) 476
AvaII GGWCC 1 cut(s) 470
AxyI CCTNAGG 1 cut(s) 425
BalI TGGCCA 1 cut(s) 1224
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 2 cut(s) 183, 449
BarI GAAGNNNNNNTAC 2 cut(s) 1138, 1170
BauI CACGAG 1 cut(s) 311
BbsI GAAGAC 3 cut(s) 30, 302, 690
Bbv12I GWGCWC 2 cut(s) 449, 649
BccI CCATC 6 cut(s) 142, 533, 545, 1133, 1138, 1190
BceAI ACGGC 1 cut(s) 976
BciT130I CCWGG 1 cut(s) 51
BclI TGATCA 1 cut(s) 259
BcoDI GTCTC 3 cut(s) 206, 767, 1028
BfaI CTAG 4 cut(s) 11, 477, 1136, 1179
BfoI RGCGCY 1 cut(s) 8
BfrI CTTAAG 1 cut(s) 911
BisI GCNGC 2 cut(s) 8, 1175
BlsI GCNGC 2 cut(s) 9, 1176
BmcAI AGTACT 1 cut(s) 661
Bme1390I CCNGG 1 cut(s) 51
Bme18I GGWCC 1 cut(s) 470
BmgT120I GGNCC 1 cut(s) 470
BmiI GGNNCC 2 cut(s) 6, 460
BmrFI CCNGG 1 cut(s) 51
BmsI GCATC 4 cut(s) 208, 370, 772, 878
BmtI GCTAGC 1 cut(s) 480
BpiI GAAGAC 3 cut(s) 30, 302, 690
BpuEI CTTGAG 2 cut(s) 89, 958
BsaBI GATNNNNATC 1 cut(s) 258
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 1028
BsaJI CCNNGG 2 cut(s) 168, 1225
BsaWI WCCGGW 1 cut(s) 829
BsaXI ACNNNNNCTCC 2 cut(s) 400, 430
Bse118I RCCGGY 1 cut(s) 886
Bse1I ACTGG 2 cut(s) 472, 887
Bse21I CCTNAGG 1 cut(s) 425
Bse3DI GCAATG 1 cut(s) 944
Bse8I GATNNNNATC 1 cut(s) 258
BseBI CCWGG 1 cut(s) 51
BseDI CCNNGG 2 cut(s) 168, 1225
BseGI GGATG 4 cut(s) 223, 385, 556, 856
BseJI GATNNNNATC 1 cut(s) 258
BseMI GCAATG 1 cut(s) 944
BseMII CTCAG 2 cut(s) 158, 405
BseNI ACTGG 2 cut(s) 472, 887
BseRI GAGGAG 2 cut(s) 389, 1275
BshFI GGCC 2 cut(s) 886, 1224
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 2 cut(s) 449, 649
BsiSI CCGG 3 cut(s) 473, 830, 887
BslFI GGGAC 1 cut(s) 888
BsmAI GTCTC 3 cut(s) 206, 767, 1028
BsmBI CGTCTC 1 cut(s) 206
BsmFI GGGAC 1 cut(s) 888
BsnI GGCC 2 cut(s) 886, 1224
Bso31I GGTCTC 1 cut(s) 1028
Bsp1286I GDGCHC 3 cut(s) 183, 449, 649
Bsp143I GATC 5 cut(s) 259, 664, 778, 855, 1163
BspACI CCGC 3 cut(s) 8, 1119, 1175
BspANI GGCC 2 cut(s) 886, 1224
BspCNI CTCAG 2 cut(s) 157, 404
BspHI TCATGA 1 cut(s) 265
BspLI GGNNCC 2 cut(s) 6, 460
BspOI GCTAGC 1 cut(s) 480
BspPI GGATC 1 cut(s) 659
BspT107I GGYRCC 1 cut(s) 4
BspTI CTTAAG 1 cut(s) 911
BspTNI GGTCTC 1 cut(s) 1028
BsrDI GCAATG 1 cut(s) 944
BsrFI RCCGGY 1 cut(s) 886
BsrI ACTGG 2 cut(s) 472, 887
BssAI RCCGGY 1 cut(s) 886
BssECI CCNNGG 2 cut(s) 168, 1225
BssMI GATC 5 cut(s) 259, 664, 778, 855, 1163
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 311
BssT1I CCWWGG 2 cut(s) 168, 1225
Bst2BI CACGAG 1 cut(s) 311
Bst2UI CCWGG 1 cut(s) 51
Bst4CI ACNGT 2 cut(s) 1022, 1327
Bst6I CTCTTC 2 cut(s) 524, 1242
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 911
BstC8I GCNNGC 2 cut(s) 478, 796
BstDEI CTNAG 5 cut(s) 144, 391, 425, 630, 738
BstF5I GGATG 4 cut(s) 223, 385, 556, 856
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 794
BstKTI GATC 5 cut(s) 262, 667, 781, 858, 1166
BstMAI GTCTC 3 cut(s) 206, 767, 1028
BstMBI GATC 5 cut(s) 259, 664, 778, 855, 1163
BstMWI GCNNNNNNNGC 2 cut(s) 89, 969
BstNI CCWGG 1 cut(s) 51
BstSCI CCNGG 1 cut(s) 49
BstV2I GAAGAC 3 cut(s) 30, 302, 690
Bsu36I CCTNAGG 1 cut(s) 425
BsuRI GGCC 2 cut(s) 886, 1224
BtsCI GGATG 4 cut(s) 223, 385, 556, 856
Cac8I GCNNGC 2 cut(s) 478, 796
CciI TCATGA 1 cut(s) 265
CfoI GCGC 2 cut(s) 7, 794
Cfr10I RCCGGY 1 cut(s) 886
Cfr13I GGNCC 1 cut(s) 470
CsiI ACCWGGT 1 cut(s) 49
Csp6I GTAC 4 cut(s) 162, 324, 660, 1321
CviAII CATG 2 cut(s) 266, 946
CviQI GTAC 4 cut(s) 162, 324, 660, 1321
DdeI CTNAG 5 cut(s) 144, 391, 425, 630, 738
DinI GGCGCC 1 cut(s) 6
DpnI GATC 5 cut(s) 261, 666, 780, 857, 1165
DpnII GATC 5 cut(s) 259, 664, 778, 855, 1163
EaeI YGGCCR 2 cut(s) 884, 1222
Eam1104I CTCTTC 2 cut(s) 524, 1242
EarI CTCTTC 2 cut(s) 524, 1242
Ecl136II GAGCTC 1 cut(s) 447
Eco130I CCWWGG 2 cut(s) 168, 1225
Eco24I GRGCYC 2 cut(s) 183, 449
Eco31I GGTCTC 1 cut(s) 1028
Eco47I GGWCC 1 cut(s) 470
Eco53kI GAGCTC 1 cut(s) 447
Eco57I CTGAAG 1 cut(s) 702
Eco81I CCTNAGG 1 cut(s) 425
EcoICRI GAGCTC 1 cut(s) 447
EcoRII CCWGG 1 cut(s) 49
EcoT14I CCWWGG 2 cut(s) 168, 1225
EcoT38I GRGCYC 2 cut(s) 183, 449
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 168, 1225
Esp3I CGTCTC 1 cut(s) 206
FaeI CATG 2 cut(s) 269, 949
FaqI GGGAC 1 cut(s) 888
FatI CATG 2 cut(s) 265, 945
FauNDI CATATG 1 cut(s) 639
FbaI TGATCA 1 cut(s) 259
FblI GTMKAC 1 cut(s) 1150
Fnu4HI GCNGC 2 cut(s) 8, 1175
FokI GGATG 4 cut(s) 230, 392, 563, 863
FriOI GRGCYC 2 cut(s) 183, 449
Fsp4HI GCNGC 2 cut(s) 8, 1175
FspBI CTAG 4 cut(s) 11, 477, 1136, 1179
GlaI GCGC 2 cut(s) 6, 793
GluI GCNGC 2 cut(s) 8, 1175
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 2 cut(s) 886, 1224
HapII CCGG 3 cut(s) 473, 830, 887
HhaI GCGC 2 cut(s) 7, 794
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 269, 949
Hin6I GCGC 2 cut(s) 5, 792
HinP1I GCGC 2 cut(s) 5, 792
HincII GTYRAC 1 cut(s) 519
HindII GTYRAC 1 cut(s) 519
HindIII AAGCTT 1 cut(s) 558
HinfI GANTC 3 cut(s) 241, 491, 618
HpaII CCGG 3 cut(s) 473, 830, 887
HphI GGTGA 3 cut(s) 557, 763, 1211
Hpy166II GTNNAC 3 cut(s) 519, 1151, 1323
Hpy188I TCNGA 4 cut(s) 147, 490, 783, 816
Hpy188III TCNNGA 8 cut(s) 245, 266, 503, 622, 772, 1061, 1161, 1274
Hpy8I GTNNAC 3 cut(s) 519, 1151, 1323
HpyAV CCTTC 6 cut(s) 112, 595, 707, 1088, 1099, 1164
HpyCH4III ACNGT 2 cut(s) 1022, 1327
HpyCH4IV ACGT 2 cut(s) 966, 1319
HpyCH4V TGCA 3 cut(s) 92, 310, 869
HpyF10VI GCNNNNNNNGC 2 cut(s) 89, 969
HpyF3I CTNAG 5 cut(s) 144, 391, 425, 630, 738
HpySE526I ACGT 2 cut(s) 966, 1319
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 269, 949
HspAI GCGC 2 cut(s) 5, 792
KasI GGCGCC 1 cut(s) 4
Ksp22I TGATCA 1 cut(s) 259
Kzo9I GATC 5 cut(s) 259, 664, 778, 855, 1163
LmnI GCTCC 2 cut(s) 464, 634
LweI GCATC 4 cut(s) 208, 370, 772, 878
MabI ACCWGGT 1 cut(s) 49
MaeI CTAG 4 cut(s) 11, 477, 1136, 1179
MaeII ACGT 2 cut(s) 966, 1319
MaeIII GTNAC 1 cut(s) 439
MalI GATC 5 cut(s) 261, 666, 780, 857, 1165
MboI GATC 5 cut(s) 259, 664, 778, 855, 1163
MfeI CAATTG 1 cut(s) 281
MhlI GDGCHC 3 cut(s) 183, 449, 649
MlsI TGGCCA 1 cut(s) 1224
MluCI AATT 8 cut(s) 184, 281, 300, 317, 349, 368, 433, 1311
MluNI TGGCCA 1 cut(s) 1224
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 250
MmeI TCCRAC 1 cut(s) 977
Mox20I TGGCCA 1 cut(s) 1224
MroXI GAANNNNTTC 1 cut(s) 1312
MscI TGGCCA 1 cut(s) 1224
MseI TTAA 6 cut(s) 132, 174, 597, 912, 1124, 1281
Msp20I TGGCCA 1 cut(s) 1224
MspCI CTTAAG 1 cut(s) 911
MspI CCGG 3 cut(s) 473, 830, 887
MspR9I CCNGG 1 cut(s) 51
MunI CAATTG 1 cut(s) 281
MvaI CCWGG 1 cut(s) 51
MwoI GCNNNNNNNGC 2 cut(s) 89, 969
NarI GGCGCC 1 cut(s) 5
NdeI CATATG 1 cut(s) 639
NdeII GATC 5 cut(s) 259, 664, 778, 855, 1163
NheI GCTAGC 1 cut(s) 476
NlaIII CATG 2 cut(s) 269, 949
NlaIV GGNNCC 2 cut(s) 6, 460
PagI TCATGA 1 cut(s) 265
PdmI GAANNNNTTC 1 cut(s) 1312
PfeI GAWTC 2 cut(s) 491, 618
PkrI GCNGC 2 cut(s) 9, 1176
PleI GAGTC 1 cut(s) 249
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 249
PsiI TTATAA 1 cut(s) 899
Psp124BI GAGCTC 1 cut(s) 449
Psp1406I AACGTT 1 cut(s) 966
Psp6I CCWGG 1 cut(s) 49
PspGI CCWGG 1 cut(s) 49
PspN4I GGNNCC 2 cut(s) 6, 460
PspPI GGNCC 1 cut(s) 470
RsaI GTAC 4 cut(s) 163, 325, 661, 1322
RsaNI GTAC 4 cut(s) 162, 324, 660, 1321
SacI GAGCTC 1 cut(s) 449
SaqAI TTAA 6 cut(s) 132, 174, 597, 912, 1124, 1281
SatI GCNGC 2 cut(s) 8, 1175
Sau3AI GATC 5 cut(s) 259, 664, 778, 855, 1163
Sau96I GGNCC 1 cut(s) 470
ScaI AGTACT 1 cut(s) 661
SchI GAGTC 1 cut(s) 250
ScrFI CCNGG 1 cut(s) 51
SduI GDGCHC 3 cut(s) 183, 449, 649
SexAI ACCWGGT 1 cut(s) 49
SfaNI GCATC 4 cut(s) 208, 370, 772, 878
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 1 cut(s) 470
SmlI CTYRAG 3 cut(s) 104, 911, 973
SmoI CTYRAG 3 cut(s) 104, 911, 973
Sse9I AATT 8 cut(s) 184, 281, 300, 317, 349, 368, 433, 1311
SsiI CCGC 3 cut(s) 8, 1119, 1175
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 2 cut(s) 67, 1219
SspMI CTAG 4 cut(s) 11, 477, 1136, 1179
SstI GAGCTC 1 cut(s) 449
StyD4I CCNGG 1 cut(s) 49
StyI CCWWGG 2 cut(s) 168, 1225
TaaI ACNGT 2 cut(s) 1022, 1327
TaiI ACGT 2 cut(s) 969, 1322
TaqI TCGA 4 cut(s) 46, 244, 771, 1060
TasI AATT 8 cut(s) 184, 281, 300, 317, 349, 368, 433, 1311
TatI WGTACW 1 cut(s) 659
TauI GCSGC 2 cut(s) 10, 1177
TfiI GAWTC 2 cut(s) 491, 618
Tru1I TTAA 6 cut(s) 132, 174, 597, 912, 1124, 1281
Tru9I TTAA 6 cut(s) 132, 174, 597, 912, 1124, 1281
TspDTI ATGAA 7 cut(s) 19, 242, 342, 605, 962, 1281, 1302
TspGWI ACGGA 2 cut(s) 230, 307
Vha464I CTTAAG 1 cut(s) 911
VpaK11BI GGWCC 1 cut(s) 470
XapI RAATTY 4 cut(s) 317, 349, 368, 1311
XmiI GTMKAC 1 cut(s) 1150
XmnI GAANNNNTTC 1 cut(s) 1312
XspI CTAG 4 cut(s) 11, 477, 1136, 1179
ZrmI AGTACT 1 cut(s) 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.