AT2G31460

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0000766
Physical Location & Seq
Forward (+)
13404776 .. 13405510
735 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G31460.1

Sequence Viewer

Length: 735 bp
ATGTCAGATTCTGCAGAGATGCAACAGCCGGAGTTACTGCTGAAATCCATGAATAAATTGAATGGGTATCATCCACAACTAATCATTCCAAAGAAGACTCTCTTCAAGTGTGATCTCGATGACAAACAGTCACGACTCCAAGTTTCTTCCATGCATATGGAAAATTCAGGCTTCTTAACGGAAGATGAAAAACGAACCATAGAGGCACAAAAGATGAAGAAACGACGGACGGCTGGTTTGAGAGTAGCTTTTATAGATCCTGAATCGCAACAGTACGTGCTTGAGTTACATAAGTGGACCAAGAGCTATGCCTTTGTTAAAGGTTGGAATAAGGTGGTTGATAAGAACGACAAAACGTTCAAGGTGGGCGACGTTTTCTCTCTCTGGGTTTTCCGTTGCGGAGGAGTGAACCCTGTTCACGACGGCGTCAATCTTTCGGGTGGCCACGCCGACTCTGTTGTTGACGGTTTGGAGCAAGGTAGTCTCTGTTTTGTTCTGGTTCCTGCAAAAGTTTCTGTTCACGACGGCAATCTTCCTCAAGATTCTGGTCACGACGGCCACAACGACAATCTTCCTCAAGATTCTGTTGAACCTAGCTCTTTTTTCGACGAGTCTTACGAGTTAAACCACCTGTTCTTTGATCAAGAAGACAGTCAAGGCTATCTTCCCGACGAAGATGAAGACTTTGGCTTCAACGATGATGGCTCGATTCGTGATTCCGGTCACTACCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

244

Amino Acids

27.59

Weight (kDa)

4.93

Isoelectric Point (pI)

58.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 8 - 115 1.8e-36 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 399
AclI AACGTT 1 cut(s) 356
AclWI GGATC 1 cut(s) 251
AcoI YGGCCR 2 cut(s) 442, 556
AcsI RAATTY 1 cut(s) 163
AcyI GRCGYC 1 cut(s) 426
AfaI GTAC 1 cut(s) 275
AgsI TTSAA 5 cut(s) 61, 106, 361, 590, 694
AhdI GACNNNNNGTC 1 cut(s) 127
AluBI AGCT 3 cut(s) 248, 306, 597
AluI AGCT 3 cut(s) 248, 306, 597
Alw26I GTCTC 1 cut(s) 488
AlwI GGATC 1 cut(s) 251
AlwNI CAGNNNCTG 1 cut(s) 11
AoxI GGCC 2 cut(s) 442, 556
ApoI RAATTY 1 cut(s) 163
ArsI GACNNNNNNTTYG 2 cut(s) 220, 252
AspS9I GGNCC 1 cut(s) 297
AvaII GGWCC 1 cut(s) 297
BalI TGGCCA 1 cut(s) 444
BbsI GAAGAC 3 cut(s) 101, 654, 687
BccI CCATC 1 cut(s) 695
BceAI ACGGC 4 cut(s) 246, 439, 541, 571
BclI TGATCA 1 cut(s) 640
BcoDI GTCTC 1 cut(s) 488
BfaI CTAG 1 cut(s) 594
BfmI CTRYAG 1 cut(s) 12
Bme18I GGWCC 1 cut(s) 297
BmeRI GACNNNNNGTC 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 297
BmiI GGNNCC 1 cut(s) 501
BmsI GCATC 1 cut(s) 9
BpiI GAAGAC 3 cut(s) 101, 654, 687
BpuEI CTTGAG 3 cut(s) 302, 522, 561
BsaAI YACGTR 1 cut(s) 277
BsaHI GRCGYC 1 cut(s) 426
BsaWI WCCGGW 1 cut(s) 719
Bse1I ACTGG 1 cut(s) 730
BseGI GGATG 1 cut(s) 70
BseNI ACTGG 1 cut(s) 730
BseRI GAGGAG 1 cut(s) 417
BshFI GGCC 2 cut(s) 444, 558
BsiSI CCGG 2 cut(s) 29, 720
BsmAI GTCTC 1 cut(s) 488
BsnI GGCC 2 cut(s) 444, 558
Bsp143I GATC 3 cut(s) 112, 256, 640
BspACI CCGC 1 cut(s) 399
BspANI GGCC 2 cut(s) 444, 558
BspLI GGNNCC 1 cut(s) 501
BspMAI CTGCAG 1 cut(s) 16
BspPI GGATC 1 cut(s) 251
BsrI ACTGG 1 cut(s) 730
BssMI GATC 3 cut(s) 112, 256, 640
BssNI GRCGYC 1 cut(s) 426
Bst4CI ACNGT 4 cut(s) 129, 273, 467, 653
Bst6I CTCTTC 1 cut(s) 107
BstACI GRCGYC 1 cut(s) 426
BstBAI YACGTR 1 cut(s) 277
BstF5I GGATG 1 cut(s) 70
BstKTI GATC 3 cut(s) 115, 259, 643
BstMAI GTCTC 1 cut(s) 488
BstMBI GATC 3 cut(s) 112, 256, 640
BstSFI CTRYAG 1 cut(s) 12
BstV2I GAAGAC 3 cut(s) 101, 654, 687
BstX2I RGATCY 1 cut(s) 256
BstXI CCANNNNNNTGG 1 cut(s) 157
BstYI RGATCY 1 cut(s) 256
BsuRI GGCC 2 cut(s) 444, 558
BtsCI GGATG 1 cut(s) 70
CaiI CAGNNNCTG 1 cut(s) 11
Cfr13I GGNCC 1 cut(s) 297
CseI GACGC 1 cut(s) 415
Csp6I GTAC 1 cut(s) 274
CviAII CATG 2 cut(s) 49, 151
CviQI GTAC 1 cut(s) 274
DpnI GATC 3 cut(s) 114, 258, 642
DpnII GATC 3 cut(s) 112, 256, 640
DriI GACNNNNNGTC 1 cut(s) 127
EaeI YGGCCR 2 cut(s) 442, 556
Eam1104I CTCTTC 1 cut(s) 107
Eam1105I GACNNNNNGTC 1 cut(s) 127
EarI CTCTTC 1 cut(s) 107
Eco47I GGWCC 1 cut(s) 297
EcoT22I ATGCAT 1 cut(s) 156
FaeI CATG 2 cut(s) 52, 154
FaiI YATR 8 cut(s) 50, 152, 156, 158, 200, 254, 291, 309
FalI AAGNNNNNCTT 4 cut(s) 86, 118, 648, 680
FatI CATG 2 cut(s) 48, 150
FauNDI CATATG 1 cut(s) 156
FbaI TGATCA 1 cut(s) 640
FokI GGATG 1 cut(s) 57
FspBI CTAG 1 cut(s) 594
HaeIII GGCC 2 cut(s) 444, 558
HapII CCGG 2 cut(s) 29, 720
HgaI GACGC 1 cut(s) 415
Hin1I GRCGYC 1 cut(s) 426
Hin1II CATG 2 cut(s) 52, 154
HincII GTYRAC 1 cut(s) 463
HindII GTYRAC 1 cut(s) 463
HpaII CCGG 2 cut(s) 29, 720
Hpy166II GTNNAC 5 cut(s) 297, 409, 418, 463, 520
Hpy188I TCNGA 1 cut(s) 7
Hpy8I GTNNAC 5 cut(s) 297, 409, 418, 463, 520
Hpy99I CGWCG 7 cut(s) 228, 374, 425, 527, 557, 611, 674
HpyCH4III ACNGT 4 cut(s) 129, 273, 467, 653
HpyCH4IV ACGT 3 cut(s) 276, 356, 372
HpyCH4V TGCA 4 cut(s) 14, 22, 154, 506
HpySE526I ACGT 3 cut(s) 276, 356, 372
Hsp92I GRCGYC 1 cut(s) 426
Hsp92II CATG 2 cut(s) 52, 154
Ksp22I TGATCA 1 cut(s) 640
Kzo9I GATC 3 cut(s) 112, 256, 640
LmnI GCTCC 1 cut(s) 472
LweI GCATC 1 cut(s) 9
MaeI CTAG 1 cut(s) 594
MaeII ACGT 3 cut(s) 276, 356, 372
MaeIII GTNAC 5 cut(s) 33, 129, 285, 548, 722
MalI GATC 3 cut(s) 114, 258, 642
MboI GATC 3 cut(s) 112, 256, 640
MflI RGATCY 1 cut(s) 256
MlsI TGGCCA 1 cut(s) 444
MluCI AATT 2 cut(s) 56, 163
MluNI TGGCCA 1 cut(s) 444
MlyI GAGTC 4 cut(s) 91, 129, 446, 620
MmeI TCCRAC 1 cut(s) 305
MnlI CCTC 4 cut(s) 196, 395, 546, 585
Mox20I TGGCCA 1 cut(s) 444
Mph1103I ATGCAT 1 cut(s) 156
MscI TGGCCA 1 cut(s) 444
MseI TTAA 3 cut(s) 176, 318, 623
MslI CAYNNNNRTG 1 cut(s) 155
Msp20I TGGCCA 1 cut(s) 444
MspI CCGG 2 cut(s) 29, 720
NdeI CATATG 1 cut(s) 156
NdeII GATC 3 cut(s) 112, 256, 640
NlaIII CATG 2 cut(s) 52, 154
NlaIV GGNNCC 1 cut(s) 501
NmuCI GTSAC 3 cut(s) 129, 548, 722
NsiI ATGCAT 1 cut(s) 156
PcsI WCGNNNNNNNCGW 2 cut(s) 561, 615
PfeI GAWTC 6 cut(s) 8, 263, 542, 581, 709, 716
PflFI GACNNNGTC 1 cut(s) 425
PleI GAGTC 4 cut(s) 91, 129, 446, 619
PpsI GAGTC 4 cut(s) 91, 129, 446, 619
Ppu21I YACGTR 1 cut(s) 277
Psp1406I AACGTT 1 cut(s) 356
PspN4I GGNNCC 1 cut(s) 501
PspPI GGNCC 1 cut(s) 297
PstI CTGCAG 1 cut(s) 16
PstNI CAGNNNCTG 1 cut(s) 11
PsuI RGATCY 1 cut(s) 256
PsyI GACNNNGTC 1 cut(s) 425
RsaI GTAC 1 cut(s) 275
RsaNI GTAC 1 cut(s) 274
RseI CAYNNNNRTG 1 cut(s) 155
SaqAI TTAA 3 cut(s) 176, 318, 623
Sau3AI GATC 3 cut(s) 112, 256, 640
Sau96I GGNCC 1 cut(s) 297
SchI GAGTC 4 cut(s) 91, 129, 446, 620
SfaNI GCATC 1 cut(s) 9
SfcI CTRYAG 1 cut(s) 12
SinI GGWCC 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 155
SmlI CTYRAG 3 cut(s) 281, 537, 576
SmoI CTYRAG 3 cut(s) 281, 537, 576
Sse9I AATT 2 cut(s) 56, 163
SsiI CCGC 1 cut(s) 399
SspMI CTAG 1 cut(s) 594
TaaI ACNGT 4 cut(s) 129, 273, 467, 653
TaiI ACGT 3 cut(s) 279, 359, 375
TaqI TCGA 3 cut(s) 117, 606, 707
TasI AATT 2 cut(s) 56, 163
TfiI GAWTC 6 cut(s) 8, 263, 542, 581, 709, 716
Tru1I TTAA 3 cut(s) 176, 318, 623
Tru9I TTAA 3 cut(s) 176, 318, 623
TseFI GTSAC 3 cut(s) 129, 548, 722
Tsp45I GTSAC 3 cut(s) 129, 548, 722
TspDTI ATGAA 4 cut(s) 65, 201, 230, 693
TspGWI ACGGA 3 cut(s) 194, 241, 383
Tth111I GACNNNGTC 1 cut(s) 425
VpaK11BI GGWCC 1 cut(s) 297
XapI RAATTY 1 cut(s) 163
XspI CTAG 1 cut(s) 594
Zsp2I ATGCAT 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.