AT2G27410

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0000766
Physical Location & Seq
Reverse (-)
11724427 .. 11725419
993 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G27410.1

Sequence Viewer

Length: 774 bp
ATGTACGCGTGCGCTCGAACAACAAAGATCAATCACTTCCGTGGAACCAGTACTACACAAAACCCTAATCGAGGTTTAGAACCGTCTCCTTCTTCTTATGTTACTCGTCGGTCCAAAGAAAAACGTCCCATTAATGTGGAAAAGAGAAGTCACAAGAAACGCAAAATCATTTGTCCTTTAGAGGAGGAACCCATTCAAACAACTCCACCGGAGTGGCTCTTGAACGTCATGAGGAGAGAAGAGAACGGTTACAATCCGAAGTTGATCTCAACGAGGCAACTGTATAAGACCGATCTCAAAAAAACTGAAGCACGTCTCTCAGTTCCTTTTAAGCAGGTGAAAACTCCGGACTTTTTGACAGAGGACGAGACAAGAATCATACATGAGAATGCCATGAAGATTCGTGACAATGGTGTGCCTGTGAATTTCGTGGATCCTGAGCTGAATAAGCATGTGCTTGAATTGAGGAAGTGGAAAATGAAAGGAAACTGGATTTATGTCTTTGTTAAAGGTTGGAAAAACGTGCTTGATGCTTGTAAGACGTTGTTTAAGGAAGACGACGTCTATCCTCTCTGGTCTTTCCGATCTGGAACAGGCAAACTCTGTTTTGCTCTCACCCCTAAAAACTCTGGCCGTGGAAACTCTCTTCCTGGCGGTGATGGCGCTTCTACTTCCGGTGAATCTGGTCAGGTACCATTACCCATTCCTCCGGCAAGATATTCTAGCAACTCCGGCCAAGGCTGTTCAGGAGAGAGCAGCTCTAGTTCTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

257

Amino Acids

29.02

Weight (kDa)

9.67

Isoelectric Point (pI)

51.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 68 - 177 1.7e-43 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 325
AatII GACGTC 1 cut(s) 564
Acc36I ACCTGC 1 cut(s) 325
Acc65I GGTACC 1 cut(s) 691
AccB1I GGYRCC 1 cut(s) 691
AccII CGCG 1 cut(s) 8
AccIII TCCGGA 1 cut(s) 346
AciI CCGC 1 cut(s) 654
AclWI GGATC 2 cut(s) 428, 441
AcoI YGGCCR 2 cut(s) 631, 733
AcsI RAATTY 1 cut(s) 424
AcuI CTGAAG 1 cut(s) 327
AcyI GRCGYC 1 cut(s) 561
AfaI GTAC 3 cut(s) 5, 52, 693
AfiI CCNNNNNNNGG 1 cut(s) 71
AflIII ACRYGT 1 cut(s) 6
AgsI TTSAA 3 cut(s) 197, 223, 461
AjiI CACGTC 1 cut(s) 314
AjnI CCWGG 1 cut(s) 649
AleI CACNNNNGTG 2 cut(s) 39, 211
AluBI AGCT 2 cut(s) 442, 759
AluI AGCT 2 cut(s) 442, 759
Alw26I GTCTC 3 cut(s) 90, 320, 362
AlwI GGATC 2 cut(s) 428, 441
Aor13HI TCCGGA 1 cut(s) 346
AoxI GGCC 2 cut(s) 631, 733
ApeKI GCWGC 1 cut(s) 756
ApoI RAATTY 1 cut(s) 424
AseI ATTAAT 1 cut(s) 132
Asp700I GAANNNNTTC 1 cut(s) 192
Asp718I GGTACC 1 cut(s) 691
AspLEI GCGC 2 cut(s) 14, 665
AspS9I GGNCC 1 cut(s) 111
AsuHPI GGTGA 4 cut(s) 349, 607, 668, 689
AvaII GGWCC 1 cut(s) 111
BamHI GGATCC 1 cut(s) 433
BanI GGYRCC 1 cut(s) 691
BbsI GAAGAC 1 cut(s) 561
BbvI GCAGC 1 cut(s) 768
BccI CCATC 1 cut(s) 653
BceAI ACGGC 1 cut(s) 618
BciT130I CCWGG 1 cut(s) 651
BcoDI GTCTC 3 cut(s) 90, 320, 362
BfaI CTAG 2 cut(s) 723, 762
BfoI RGCGCY 1 cut(s) 666
BfuAI ACCTGC 1 cut(s) 325
BisI GCNGC 1 cut(s) 757
BlsI GCNGC 1 cut(s) 758
BmcAI AGTACT 1 cut(s) 52
Bme1390I CCNGG 1 cut(s) 651
Bme18I GGWCC 1 cut(s) 111
BmgBI CACGTC 1 cut(s) 314
BmgT120I GGNCC 1 cut(s) 111
BmiI GGNNCC 4 cut(s) 46, 189, 435, 693
BmrFI CCNGG 1 cut(s) 651
BmsI GCATC 1 cut(s) 520
BpiI GAAGAC 1 cut(s) 561
BplI GAGNNNNNCTC 1 cut(s) 743
Bpu10I CCTNAGC 1 cut(s) 438
BsaHI GRCGYC 1 cut(s) 561
BsaJI CCNNGG 3 cut(s) 40, 634, 736
BsaWI WCCGGW 3 cut(s) 208, 346, 674
Bsc4I CCNNNNNNNGG 1 cut(s) 71
Bse1I ACTGG 2 cut(s) 48, 494
BseAI TCCGGA 1 cut(s) 346
BseBI CCWGG 1 cut(s) 651
BseDI CCNNGG 3 cut(s) 40, 634, 736
BseLI CCNNNNNNNGG 1 cut(s) 71
BseMII CTCAG 2 cut(s) 333, 429
BseNI ACTGG 2 cut(s) 48, 494
BseRI GAGGAG 2 cut(s) 197, 247
BseXI GCAGC 1 cut(s) 768
Bsh1236I CGCG 1 cut(s) 8
BshFI GGCC 2 cut(s) 633, 735
BshNI GGYRCC 1 cut(s) 691
BsiSI CCGG 5 cut(s) 209, 347, 675, 710, 732
BslFI GGGAC 1 cut(s) 111
BslI CCNNNNNNNGG 1 cut(s) 71
BsmAI GTCTC 3 cut(s) 90, 320, 362
BsmBI CGTCTC 2 cut(s) 90, 320
BsmFI GGGAC 1 cut(s) 111
BsmI GAATGC 1 cut(s) 394
BsnI GGCC 2 cut(s) 633, 735
Bsp13I TCCGGA 1 cut(s) 346
Bsp143I GATC 5 cut(s) 27, 264, 292, 433, 584
BspACI CCGC 1 cut(s) 654
BspANI GGCC 2 cut(s) 633, 735
BspCNI CTCAG 2 cut(s) 332, 430
BspEI TCCGGA 1 cut(s) 346
BspFNI CGCG 1 cut(s) 8
BspHI TCATGA 1 cut(s) 228
BspLI GGNNCC 4 cut(s) 46, 189, 435, 693
BspMI ACCTGC 1 cut(s) 325
BspPI GGATC 2 cut(s) 428, 441
BspT107I GGYRCC 1 cut(s) 691
BsrI ACTGG 2 cut(s) 48, 494
BssECI CCNNGG 3 cut(s) 40, 634, 736
BssMI GATC 5 cut(s) 27, 264, 292, 433, 584
BssNI GRCGYC 1 cut(s) 561
BssT1I CCWWGG 1 cut(s) 736
Bst2UI CCWGG 1 cut(s) 651
Bst4CI ACNGT 3 cut(s) 84, 248, 282
Bst6I CTCTTC 2 cut(s) 234, 651
BstACI GRCGYC 1 cut(s) 561
BstC8I GCNNGC 1 cut(s) 10
BstDEI CTNAG 2 cut(s) 319, 438
BstDSI CCRYGG 2 cut(s) 40, 634
BstENI CCTNNNNNAGG 1 cut(s) 69
BstFNI CGCG 1 cut(s) 8
BstH2I RGCGCY 1 cut(s) 666
BstHHI GCGC 2 cut(s) 14, 665
BstKTI GATC 5 cut(s) 30, 267, 295, 436, 587
BstMAI GTCTC 3 cut(s) 90, 320, 362
BstMBI GATC 5 cut(s) 27, 264, 292, 433, 584
BstMWI GCNNNNNNNGC 3 cut(s) 448, 660, 732
BstNI CCWGG 1 cut(s) 651
BstNSI RCATGY 1 cut(s) 455
BstSCI CCNGG 1 cut(s) 649
BstUI CGCG 1 cut(s) 8
BstV1I GCAGC 1 cut(s) 768
BstV2I GAAGAC 1 cut(s) 561
BstX2I RGATCY 1 cut(s) 433
BstXI CCANNNNNNTGG 2 cut(s) 136, 213
BstYI RGATCY 1 cut(s) 433
BsuRI GGCC 2 cut(s) 633, 735
BtgI CCRYGG 2 cut(s) 40, 634
BtrI CACGTC 1 cut(s) 314
BveI ACCTGC 1 cut(s) 325
Cac8I GCNNGC 1 cut(s) 10
CciI TCATGA 1 cut(s) 228
CfoI GCGC 2 cut(s) 14, 665
Cfr13I GGNCC 1 cut(s) 111
Csp6I GTAC 3 cut(s) 4, 51, 692
CviAII CATG 4 cut(s) 229, 383, 394, 452
CviJI RGCY 6 cut(s) 217, 442, 633, 735, 741, 759
CviKI_1 RGCY 6 cut(s) 217, 442, 633, 735, 741, 759
CviQI GTAC 3 cut(s) 4, 51, 692
DdeI CTNAG 2 cut(s) 319, 438
DpnI GATC 5 cut(s) 29, 266, 294, 435, 586
DpnII GATC 5 cut(s) 27, 264, 292, 433, 584
EaeI YGGCCR 2 cut(s) 631, 733
Eam1104I CTCTTC 2 cut(s) 234, 651
EarI CTCTTC 2 cut(s) 234, 651
Eco130I CCWWGG 1 cut(s) 736
Eco47I GGWCC 1 cut(s) 111
Eco57I CTGAAG 1 cut(s) 327
EcoNI CCTNNNNNAGG 1 cut(s) 69
EcoRII CCWGG 1 cut(s) 649
EcoT14I CCWWGG 1 cut(s) 736
ErhI CCWWGG 1 cut(s) 736
Esp3I CGTCTC 2 cut(s) 90, 320
FaeI CATG 4 cut(s) 232, 386, 397, 455
FaiI YATR 8 cut(s) 99, 230, 285, 380, 384, 395, 453, 498
FaqI GGGAC 1 cut(s) 111
FatI CATG 4 cut(s) 228, 382, 393, 451
Fnu4HI GCNGC 1 cut(s) 757
Fsp4HI GCNGC 1 cut(s) 757
FspBI CTAG 2 cut(s) 723, 762
GlaI GCGC 2 cut(s) 13, 664
GluI GCNGC 1 cut(s) 757
HaeII RGCGCY 1 cut(s) 666
HaeIII GGCC 2 cut(s) 633, 735
HapII CCGG 5 cut(s) 209, 347, 675, 710, 732
HhaI GCGC 2 cut(s) 14, 665
Hin1I GRCGYC 1 cut(s) 561
Hin1II CATG 4 cut(s) 232, 386, 397, 455
Hin6I GCGC 2 cut(s) 12, 663
HinP1I GCGC 2 cut(s) 12, 663
HinfI GANTC 3 cut(s) 375, 400, 680
HpaII CCGG 5 cut(s) 209, 347, 675, 710, 732
HphI GGTGA 4 cut(s) 349, 607, 668, 689
Hpy188I TCNGA 2 cut(s) 258, 584
Hpy188III TCNNGA 7 cut(s) 220, 229, 347, 404, 437, 588, 747
Hpy99I CGWCG 2 cut(s) 111, 563
HpyAV CCTTC 1 cut(s) 99
HpyCH4III ACNGT 3 cut(s) 84, 248, 282
HpyCH4IV ACGT 6 cut(s) 124, 225, 313, 522, 542, 561
HpyF10VI GCNNNNNNNGC 3 cut(s) 448, 660, 732
HpyF3I CTNAG 2 cut(s) 319, 438
HpySE526I ACGT 6 cut(s) 124, 225, 313, 522, 542, 561
Hsp92I GRCGYC 1 cut(s) 561
Hsp92II CATG 4 cut(s) 232, 386, 397, 455
HspAI GCGC 2 cut(s) 12, 663
Kpn2I TCCGGA 1 cut(s) 346
KpnI GGTACC 1 cut(s) 695
Kzo9I GATC 5 cut(s) 27, 264, 292, 433, 584
Lsp1109I GCAGC 1 cut(s) 768
LweI GCATC 1 cut(s) 520
MaeI CTAG 2 cut(s) 723, 762
MaeII ACGT 6 cut(s) 124, 225, 313, 522, 542, 561
MaeIII GTNAC 4 cut(s) 100, 149, 248, 404
MalI GATC 5 cut(s) 29, 266, 294, 435, 586
MboI GATC 5 cut(s) 27, 264, 292, 433, 584
MboII GAAGA 6 cut(s) 84, 251, 409, 566, 638, 759
MflI RGATCY 1 cut(s) 433
MluCI AATT 2 cut(s) 424, 461
MluI ACGCGT 1 cut(s) 6
MmeI TCCRAC 1 cut(s) 494
MnlI CCTC 9 cut(s) 65, 175, 178, 225, 267, 355, 459, 579, 717
MroI TCCGGA 1 cut(s) 346
MroXI GAANNNNTTC 1 cut(s) 192
MseI TTAA 4 cut(s) 132, 330, 507, 549
MslI CAYNNNNRTG 4 cut(s) 39, 134, 211, 387
MspI CCGG 5 cut(s) 209, 347, 675, 710, 732
MspR9I CCNGG 1 cut(s) 651
Mva1269I GAATGC 1 cut(s) 394
MvaI CCWGG 1 cut(s) 651
MvnI CGCG 1 cut(s) 8
MwoI GCNNNNNNNGC 3 cut(s) 448, 660, 732
NdeII GATC 5 cut(s) 27, 264, 292, 433, 584
NlaIII CATG 4 cut(s) 232, 386, 397, 455
NlaIV GGNNCC 4 cut(s) 46, 189, 435, 693
NmuCI GTSAC 2 cut(s) 149, 404
NspI RCATGY 1 cut(s) 455
OliI CACNNNNGTG 2 cut(s) 39, 211
PagI TCATGA 1 cut(s) 228
PaqCI CACCTGC 1 cut(s) 325
PctI GAATGC 1 cut(s) 394
PdmI GAANNNNTTC 1 cut(s) 192
PfeI GAWTC 3 cut(s) 375, 400, 680
PflFI GACNNNGTC 1 cut(s) 560
PkrI GCNGC 1 cut(s) 758
PshBI ATTAAT 1 cut(s) 132
Psp6I CCWGG 1 cut(s) 649
PspGI CCWGG 1 cut(s) 649
PspN4I GGNNCC 4 cut(s) 46, 189, 435, 693
PspPI GGNCC 1 cut(s) 111
PsrI GAACNNNNNNTAC 2 cut(s) 37, 69
PsuI RGATCY 1 cut(s) 433
PsyI GACNNNGTC 1 cut(s) 560
RsaI GTAC 3 cut(s) 5, 52, 693
RsaNI GTAC 3 cut(s) 4, 51, 692
RseI CAYNNNNRTG 4 cut(s) 39, 134, 211, 387
SaqAI TTAA 4 cut(s) 132, 330, 507, 549
SatI GCNGC 1 cut(s) 757
Sau3AI GATC 5 cut(s) 27, 264, 292, 433, 584
Sau96I GGNCC 1 cut(s) 111
ScaI AGTACT 1 cut(s) 52
ScrFI CCNGG 1 cut(s) 651
SfaNI GCATC 1 cut(s) 520
SinI GGWCC 1 cut(s) 111
SmiMI CAYNNNNRTG 4 cut(s) 39, 134, 211, 387
Sse9I AATT 2 cut(s) 424, 461
SsiI CCGC 1 cut(s) 654
SspMI CTAG 2 cut(s) 723, 762
StyD4I CCNGG 1 cut(s) 649
StyI CCWWGG 1 cut(s) 736
TaaI ACNGT 3 cut(s) 84, 248, 282
TaiI ACGT 6 cut(s) 127, 228, 316, 525, 545, 564
TaqI TCGA 2 cut(s) 16, 70
TaqII GACCGA 2 cut(s) 99, 305
TasI AATT 2 cut(s) 424, 461
TatI WGTACW 1 cut(s) 50
TfiI GAWTC 3 cut(s) 375, 400, 680
Tru1I TTAA 4 cut(s) 132, 330, 507, 549
Tru9I TTAA 4 cut(s) 132, 330, 507, 549
TseFI GTSAC 2 cut(s) 149, 404
TseI GCWGC 1 cut(s) 756
Tsp45I GTSAC 2 cut(s) 149, 404
TspDTI ATGAA 2 cut(s) 410, 494
TspGWI ACGGA 1 cut(s) 29
Tth111I GACNNNGTC 1 cut(s) 560
VpaK11BI GGWCC 1 cut(s) 111
VspI ATTAAT 1 cut(s) 132
XagI CCTNNNNNAGG 1 cut(s) 69
XapI RAATTY 1 cut(s) 424
XceI RCATGY 1 cut(s) 455
XmnI GAANNNNTTC 1 cut(s) 192
XspI CTAG 2 cut(s) 723, 762
ZraI GACGTC 1 cut(s) 562
ZrmI AGTACT 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.