Prupe.2G278500_v2.0.a1

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000766
Physical Location & Seq
Forward (+)
27893607 .. 27895071
1465 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G278500.1

Sequence Viewer

Length: 1215 bp
ATGGGGTGGCATTCAAGCACTGACAGCGACTTTGATGGCATCTTCAATCGCAAGTTGACTAATATGGGTTGGTTGCTTGAGATTGTTAGGTTTGATACTGAGAAATATATGCAGGAAGACCTTTTGAAGCAAACGAGTAACATCAACAAAGAACCAAATTGTCCAGCAGCAGAGAGAGAATCTGAAATTGCTGAGGCCGCACACCCAGTTGCCCCATCCATGGTGTCGTCTGAGTCCAAGGGAGAAGGTTTGATGAAGGAAAAGGCATTCAAGAACCCTGCACTAATAGAGCCAAAGAAACAAAGATCATCGGATGGACCAAAGCTTATGCACACTGCTATTGCTTCTGCGAGAAAGACTAATTCTTTGAAAAGAAAATTCCAGAGCAGAGATGATGATGATAGTACTAATTGGGCGCCTGTTGAATGCAAGAAGCAAAGGATGATGATGATGTTGAAGAAGAAAAATGCCAATAAAGGCACCAGCTCGATCAACAAGATCACTCCGAAGAAAAAAGAAGAACTGAGTAGTCTTTCCTCATCAGCTCATCATGACTTGCCTGAAGCATTTAAGAGAAAAATTGCCAGTTTGGGTGGGACTAAAGTGGCTTTGGTGATACAGAAAAAACTGAGGAAAACTGATCTAAGTAAAAGTGGTAACAGGATGTCAATGCCTCTGAACCAAATCTCGTCGACTGATTTCTTGGAAGACGATGAGAGAGGTAGGCTTGAGAAAGAGGAGACAATGAAGGTTCAACTGATAGACCCTGGTCTTGTACAAGGAGATATAAACTTGAGACGGTGGAACATGAAAAAGATGAATGGGAAAAACAGCAAAATCTATGTGTTGAGAACTCAGTGGAGTGATTTTGCTAAGCGAAACCATCTGAAAGAAAAAGATCTTGTTCAAGTTTGGTCGTTTCGAGTTAACGGAGCTCTTTACTTGGCTCTTGCTTTGGTTAACGAGAGTTGTGGTGGTGATCAAAAGGAGAGTGATGATGGTGCGTATAAGAGTAGTGTTGTTGATGGTGAAATTAGTAGTGGTGGTAGTCACGGTGGTGCAGAGGTAATTGAAGAAAGGAGAGATGGGAATAATATTGGGGAAAGTAGTGTGACCAATGGGTCTAATCATACAATAAGTCCAAGCCATAGTGAAAGTGGCAGCAAGCATGAACAAGCTGCACGCATGGAAGATAGAAGACGAAAGGTTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

405

Amino Acids

45.26

Weight (kDa)

9.3

Isoelectric Point (pI)

53.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1120
AccB1I GGYRCC 2 cut(s) 415, 479
AccI GTMKAC 1 cut(s) 692
AciI CCGC 1 cut(s) 198
AcsI RAATTY 1 cut(s) 377
AcuI CTGAAG 1 cut(s) 582
AcyI GRCGYC 1 cut(s) 416
AfaI GTAC 2 cut(s) 406, 777
AfiI CCNNNNNNNGG 1 cut(s) 220
AjnI CCWGG 1 cut(s) 766
AleI CACNNNNGTG 1 cut(s) 1056
AluBI AGCT 5 cut(s) 325, 486, 545, 935, 1178
AluI AGCT 5 cut(s) 325, 486, 545, 935, 1178
Alw21I GWGCWC 1 cut(s) 937
Alw26I GTCTC 2 cut(s) 734, 790
AoxI GGCC 1 cut(s) 195
ApeKI GCWGC 3 cut(s) 167, 1161, 1178
ApoI RAATTY 1 cut(s) 377
ArsI GACNNNNNNTTYG 4 cut(s) 14, 46, 349, 381
AspLEI GCGC 1 cut(s) 418
AspS9I GGNCC 1 cut(s) 317
AsuHPI GGTGA 3 cut(s) 625, 989, 1040
AvaII GGWCC 1 cut(s) 317
BanI GGYRCC 2 cut(s) 415, 479
BanII GRGCYC 1 cut(s) 937
BbsI GAAGAC 3 cut(s) 123, 714, 1204
Bbv12I GWGCWC 1 cut(s) 937
BbvCI CCTCAGC 1 cut(s) 192
BbvI GCAGC 3 cut(s) 179, 1165, 1173
BccI CCATC 7 cut(s) 29, 223, 308, 891, 992, 1019, 1079
BciT130I CCWGG 1 cut(s) 768
BclI TGATCA 1 cut(s) 979
BcoDI GTCTC 2 cut(s) 734, 790
BfoI RGCGCY 1 cut(s) 419
BglII AGATCT 1 cut(s) 898
BisI GCNGC 4 cut(s) 168, 198, 1162, 1179
BlpI GCTNAGC 1 cut(s) 873
BlsI GCNGC 4 cut(s) 169, 199, 1163, 1180
BmcAI AGTACT 1 cut(s) 406
Bme1390I CCNGG 1 cut(s) 768
Bme18I GGWCC 1 cut(s) 317
BmgT120I GGNCC 1 cut(s) 317
BmiI GGNNCC 2 cut(s) 417, 481
BmrFI CCNGG 1 cut(s) 768
BmrI ACTGGG 1 cut(s) 200
BmsI GCATC 1 cut(s) 48
BmuI ACTGGG 1 cut(s) 200
BoxI GACNNNNGTC 1 cut(s) 768
BpiI GAAGAC 3 cut(s) 123, 714, 1204
Bpu10I CCTNAGC 1 cut(s) 192
Bpu1102I GCTNAGC 1 cut(s) 873
BpuEI CTTGAG 3 cut(s) 98, 749, 814
BsaHI GRCGYC 1 cut(s) 416
BsaJI CCNNGG 3 cut(s) 219, 237, 766
Bsc4I CCNNNNNNNGG 1 cut(s) 220
Bse1I ACTGG 2 cut(s) 206, 585
BseBI CCWGG 1 cut(s) 768
BseDI CCNNGG 3 cut(s) 219, 237, 766
BseGI GGATG 4 cut(s) 215, 319, 447, 669
BseLI CCNNNNNNNGG 1 cut(s) 220
BseMII CTCAG 6 cut(s) 90, 183, 222, 515, 620, 869
BseNI ACTGG 2 cut(s) 206, 585
BseRI GAGGAG 1 cut(s) 752
BseXI GCAGC 3 cut(s) 179, 1165, 1173
BsgI GTGCAG 3 cut(s) 264, 1080, 1164
BshFI GGCC 1 cut(s) 197
BshNI GGYRCC 2 cut(s) 415, 479
BsiHKAI GWGCWC 1 cut(s) 937
BslFI GGGAC 1 cut(s) 610
BslI CCNNNNNNNGG 1 cut(s) 220
BsmAI GTCTC 2 cut(s) 734, 790
BsmBI CGTCTC 1 cut(s) 790
BsmFI GGGAC 1 cut(s) 610
BsmI GAATGC 3 cut(s) 10, 266, 431
BsnI GGCC 1 cut(s) 197
Bsp1286I GDGCHC 1 cut(s) 937
Bsp1407I TGTACA 1 cut(s) 775
Bsp143I GATC 6 cut(s) 305, 489, 498, 640, 898, 979
Bsp1720I GCTNAGC 1 cut(s) 873
Bsp19I CCATGG 1 cut(s) 219
BspACI CCGC 1 cut(s) 198
BspANI GGCC 1 cut(s) 197
BspCNI CTCAG 6 cut(s) 91, 184, 223, 516, 621, 868
BspHI TCATGA 1 cut(s) 550
BspLI GGNNCC 2 cut(s) 417, 481
BspT107I GGYRCC 2 cut(s) 415, 479
BsrGI TGTACA 1 cut(s) 775
BsrI ACTGG 2 cut(s) 206, 585
BssECI CCNNGG 3 cut(s) 219, 237, 766
BssMI GATC 6 cut(s) 305, 489, 498, 640, 898, 979
BssNI GRCGYC 1 cut(s) 416
BssT1I CCWWGG 2 cut(s) 219, 237
Bst2UI CCWGG 1 cut(s) 768
Bst4CI ACNGT 2 cut(s) 801, 1055
BstACI GRCGYC 1 cut(s) 416
BstAUI TGTACA 1 cut(s) 775
BstC8I GCNNGC 2 cut(s) 1166, 1183
BstDEI CTNAG 8 cut(s) 99, 192, 231, 524, 629, 644, 855, 873
BstDSI CCRYGG 1 cut(s) 219
BstF5I GGATG 4 cut(s) 215, 319, 447, 669
BstH2I RGCGCY 1 cut(s) 419
BstHHI GCGC 1 cut(s) 418
BstKTI GATC 6 cut(s) 308, 492, 501, 643, 901, 982
BstMAI GTCTC 2 cut(s) 734, 790
BstMBI GATC 6 cut(s) 305, 489, 498, 640, 898, 979
BstMWI GCNNNNNNNGC 2 cut(s) 24, 197
BstNI CCWGG 1 cut(s) 768
BstPAI GACNNNNGTC 1 cut(s) 768
BstSCI CCNGG 1 cut(s) 766
BstV1I GCAGC 3 cut(s) 179, 1165, 1173
BstV2I GAAGAC 3 cut(s) 123, 714, 1204
BstX2I RGATCY 1 cut(s) 898
BstYI RGATCY 1 cut(s) 898
BsuRI GGCC 1 cut(s) 197
BtgI CCRYGG 1 cut(s) 219
BtsCI GGATG 4 cut(s) 215, 319, 447, 669
BtsI GCAGTG 1 cut(s) 333
BtsIMutI CAGTG 3 cut(s) 18, 333, 863
Cac8I GCNNGC 2 cut(s) 1166, 1183
CciI TCATGA 1 cut(s) 550
CfoI GCGC 1 cut(s) 418
Cfr13I GGNCC 1 cut(s) 317
Csp6I GTAC 2 cut(s) 405, 776
CviAII CATG 5 cut(s) 220, 551, 808, 1169, 1186
CviQI GTAC 2 cut(s) 405, 776
DdeI CTNAG 8 cut(s) 99, 192, 231, 524, 629, 644, 855, 873
DinI GGCGCC 1 cut(s) 417
DpnI GATC 6 cut(s) 307, 491, 500, 642, 900, 981
DpnII GATC 6 cut(s) 305, 489, 498, 640, 898, 979
DrdI GACNNNNNNGTC 1 cut(s) 1120
DseDI GACNNNNNNGTC 1 cut(s) 1120
Ecl136II GAGCTC 1 cut(s) 935
Eco130I CCWWGG 2 cut(s) 219, 237
Eco24I GRGCYC 1 cut(s) 937
Eco47I GGWCC 1 cut(s) 317
Eco53kI GAGCTC 1 cut(s) 935
Eco57I CTGAAG 1 cut(s) 582
EcoICRI GAGCTC 1 cut(s) 935
EcoRII CCWGG 1 cut(s) 766
EcoT14I CCWWGG 2 cut(s) 219, 237
EcoT38I GRGCYC 1 cut(s) 937
EgeI GGCGCC 1 cut(s) 417
EheI GGCGCC 1 cut(s) 417
ErhI CCWWGG 2 cut(s) 219, 237
Esp3I CGTCTC 1 cut(s) 790
FaeI CATG 5 cut(s) 223, 554, 811, 1172, 1189
FaqI GGGAC 1 cut(s) 610
FatI CATG 5 cut(s) 219, 550, 807, 1168, 1185
FbaI TGATCA 1 cut(s) 979
FblI GTMKAC 1 cut(s) 692
Fnu4HI GCNGC 4 cut(s) 168, 198, 1162, 1179
FokI GGATG 4 cut(s) 202, 326, 454, 676
FriOI GRGCYC 1 cut(s) 937
Fsp4HI GCNGC 4 cut(s) 168, 198, 1162, 1179
GlaI GCGC 1 cut(s) 417
GluI GCNGC 4 cut(s) 168, 198, 1162, 1179
HaeII RGCGCY 1 cut(s) 419
HaeIII GGCC 1 cut(s) 197
HhaI GCGC 1 cut(s) 418
Hin1I GRCGYC 1 cut(s) 416
Hin1II CATG 5 cut(s) 223, 554, 811, 1172, 1189
Hin6I GCGC 1 cut(s) 416
HinP1I GCGC 1 cut(s) 416
HincII GTYRAC 4 cut(s) 57, 693, 928, 961
HindII GTYRAC 4 cut(s) 57, 693, 928, 961
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 2 cut(s) 179, 233
HpaI GTTAAC 2 cut(s) 928, 961
HphI GGTGA 3 cut(s) 625, 989, 1040
Hpy166II GTNNAC 4 cut(s) 57, 693, 928, 961
Hpy188I TCNGA 6 cut(s) 184, 232, 313, 507, 678, 888
Hpy188III TCNNGA 3 cut(s) 271, 382, 551
Hpy8I GTNNAC 4 cut(s) 57, 693, 928, 961
Hpy99I CGWCG 1 cut(s) 694
HpyAV CCTTC 3 cut(s) 239, 250, 742
HpyCH4III ACNGT 2 cut(s) 801, 1055
HpyCH4V TGCA 6 cut(s) 112, 281, 331, 429, 1061, 1181
HpyF10VI GCNNNNNNNGC 2 cut(s) 24, 197
HpyF3I CTNAG 8 cut(s) 99, 192, 231, 524, 629, 644, 855, 873
Hsp92I GRCGYC 1 cut(s) 416
Hsp92II CATG 5 cut(s) 223, 554, 811, 1172, 1189
HspAI GCGC 1 cut(s) 416
KasI GGCGCC 1 cut(s) 415
Ksp22I TGATCA 1 cut(s) 979
KspAI GTTAAC 2 cut(s) 928, 961
Kzo9I GATC 6 cut(s) 305, 489, 498, 640, 898, 979
LmnI GCTCC 1 cut(s) 932
Lsp1109I GCAGC 3 cut(s) 179, 1165, 1173
LweI GCATC 1 cut(s) 48
MaeIII GTNAC 4 cut(s) 137, 656, 1049, 1111
MalI GATC 6 cut(s) 307, 491, 500, 642, 900, 981
MboI GATC 6 cut(s) 305, 489, 498, 640, 898, 979
MflI RGATCY 1 cut(s) 898
MhlI GDGCHC 1 cut(s) 937
MluCI AATT 8 cut(s) 157, 186, 361, 377, 409, 579, 1032, 1068
Mly113I GGCGCC 1 cut(s) 416
MlyI GAGTC 1 cut(s) 242
MnlI CCTC 7 cut(s) 187, 547, 624, 684, 713, 730, 1057
MseI TTAA 4 cut(s) 570, 927, 960, 1213
MslI CAYNNNNRTG 1 cut(s) 1056
MspR9I CCNGG 1 cut(s) 768
Mva1269I GAATGC 3 cut(s) 10, 266, 431
MvaI CCWGG 1 cut(s) 768
MwoI GCNNNNNNNGC 2 cut(s) 24, 197
NarI GGCGCC 1 cut(s) 416
NcoI CCATGG 1 cut(s) 219
NdeII GATC 6 cut(s) 305, 489, 498, 640, 898, 979
NlaIII CATG 5 cut(s) 223, 554, 811, 1172, 1189
NlaIV GGNNCC 2 cut(s) 417, 481
NmuCI GTSAC 2 cut(s) 1049, 1111
OliI CACNNNNGTG 1 cut(s) 1056
PagI TCATGA 1 cut(s) 550
PctI GAATGC 3 cut(s) 10, 266, 431
PfeI GAWTC 1 cut(s) 179
PkrI GCNGC 4 cut(s) 169, 199, 1163, 1180
PleI GAGTC 1 cut(s) 241
PluTI GGCGCC 1 cut(s) 419
PpsI GAGTC 1 cut(s) 241
PshAI GACNNNNGTC 1 cut(s) 768
Psp124BI GAGCTC 1 cut(s) 937
Psp6I CCWGG 1 cut(s) 766
PspGI CCWGG 1 cut(s) 766
PspN4I GGNNCC 2 cut(s) 417, 481
PspPI GGNCC 1 cut(s) 317
PsuI RGATCY 1 cut(s) 898
RsaI GTAC 2 cut(s) 406, 777
RsaNI GTAC 2 cut(s) 405, 776
RseI CAYNNNNRTG 1 cut(s) 1056
SacI GAGCTC 1 cut(s) 937
SalI GTCGAC 1 cut(s) 691
SaqAI TTAA 4 cut(s) 570, 927, 960, 1213
SatI GCNGC 4 cut(s) 168, 198, 1162, 1179
Sau3AI GATC 6 cut(s) 305, 489, 498, 640, 898, 979
Sau96I GGNCC 1 cut(s) 317
ScaI AGTACT 1 cut(s) 406
SchI GAGTC 1 cut(s) 242
ScrFI CCNGG 1 cut(s) 768
SduI GDGCHC 1 cut(s) 937
SfaNI GCATC 1 cut(s) 48
SfoI GGCGCC 1 cut(s) 417
SinI GGWCC 1 cut(s) 317
SmiMI CAYNNNNRTG 1 cut(s) 1056
SmlI CTYRAG 3 cut(s) 77, 728, 793
SmoI CTYRAG 3 cut(s) 77, 728, 793
Sse9I AATT 8 cut(s) 157, 186, 361, 377, 409, 579, 1032, 1068
SsiI CCGC 1 cut(s) 198
SspDI GGCGCC 1 cut(s) 415
SspI AATATT 1 cut(s) 1096
SstI GAGCTC 1 cut(s) 937
StyD4I CCNGG 1 cut(s) 766
StyI CCWWGG 2 cut(s) 219, 237
TaaI ACNGT 2 cut(s) 801, 1055
TaqI TCGA 3 cut(s) 488, 692, 922
TasI AATT 8 cut(s) 157, 186, 361, 377, 409, 579, 1032, 1068
TatI WGTACW 2 cut(s) 404, 775
TauI GCSGC 1 cut(s) 200
TfiI GAWTC 1 cut(s) 179
Tru1I TTAA 4 cut(s) 570, 927, 960, 1213
Tru9I TTAA 4 cut(s) 570, 927, 960, 1213
TscAI CASTG 3 cut(s) 25, 340, 863
TseFI GTSAC 2 cut(s) 1049, 1111
TseI GCWGC 3 cut(s) 167, 1161, 1178
Tsp45I GTSAC 2 cut(s) 1049, 1111
TspDTI ATGAA 5 cut(s) 269, 761, 824, 833, 1185
TspGWI ACGGA 1 cut(s) 945
TspRI CASTG 3 cut(s) 25, 340, 863
VpaK11BI GGWCC 1 cut(s) 317
XapI RAATTY 1 cut(s) 377
XcmI CCANNNNNNNNNTGG 1 cut(s) 1154
XmiI GTMKAC 1 cut(s) 692
ZrmI AGTACT 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.