RLG00000026304

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000766
Physical Location & Seq
Reverse (-)
2006341 .. 2013653
7313 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026304

Sequence Viewer

Length: 1122 bp
ATGGCGCCGCTAGTGAGTATGGAAGACTTCATAGAAAAACAACTTCGACCTGGTTCAACCTTGAATATTTTGGGTCAGGTTGCTTGTGTTGCACGGGAGAAACTCAAGCAAAGGAGGAAGGAGAAAAGTATTAAAGTCAAAACTCAGATGGGTAGTAGTAGTACCCCCAAGATTAAAGAGCCCAATTATGTAACTGAAACATGTTCCGAAGAGAGGGATGCTGATATGAACAAAAGAAAGAGTCGAGAGGACGATGTTGATCATCATGACAAAGGTTTTCAATTGAAACTGACAATTTTTTTGCATGAGAAATTTGGTACTACCTCCAAGACTAAAGAAACAAATTCATCGATGGGTAAAGAAGTTGTGGTGGATGCATTATACTCGGACGTTGGCACAATGAGGAGAGAGAGGTATTTCCTTATGACAAATTATATAACGAGTTCTGATTCAGGGTTTCAGGAAAAAAAAGGGTTGACAAAGAAGAGTATTGACGGTGGTGACGATAATAAAGATTGGAGTTTGAAGGCCAAGCCAAAGAATATGAAGATTAAGAAGGAAAAGGGGTTGGGATTCAAGAGGCCTAAGGAGCATATGAGAGCACCAAGATATAGTACTGATCCTAATTATGACTTGCTTGAAGGCTTGAAAAGGAAGATTGAAACTTTGGAAGGTATTGAAAGTTTCGAAAAGGTCAGGATGACTATGGTGATAGAGAACCATTTGTTCGAGACTGATCTGACTGATAGCGCAAACCGGTTGTCAATGCCTCTGAAACAAATCAAACTGGAGAGTTTCTTGGACGAGGATGAGATAAAGTTTCTTGCATCTCAACAAGACTGGCCGGTTTCAGTTATAAGTCCCAAACTTGAGGATGAAAGCCTAACTTTTAGGCAGTGGAACATGAATAAAAATAACAGAAACGTTAGCTCAAGTTATGTGTTGAAAACAGGTTGGAACGAACTTCACAAGAAAAAGGCGGGTGGTTCAGTTAGTGATTGGGATGGGTGTAGTCTACCTTCTTTCCGATCAACATTGCCGCCTAGTAACAATGGTGTTGACGGTGAAACTATAGTTGTGATATTGGCCAAGGAACAAAAACAAAAGAACAAGAGAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

374

Amino Acids

42.75

Weight (kDa)

9.3

Isoelectric Point (pI)

45.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 235 - 322 3.9e-13 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 857
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 1015
AciI CCGC 3 cut(s) 8, 980, 1040
AclI AACGTT 1 cut(s) 924
AclWI GGATC 1 cut(s) 614
AcoI YGGCCR 2 cut(s) 842, 1086
AcsI RAATTY 2 cut(s) 311, 343
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 163, 319, 616
AfiI CCNNNNNNNGG 1 cut(s) 213
AflIII ACRYGT 1 cut(s) 200
AgeI ACCGGT 1 cut(s) 756
AjnI CCWGG 1 cut(s) 49
AluBI AGCT 1 cut(s) 930
AluI AGCT 1 cut(s) 930
Alw21I GWGCWC 1 cut(s) 604
Alw26I GTCTC 1 cut(s) 725
AlwI GGATC 1 cut(s) 614
AoxI GGCC 4 cut(s) 528, 581, 842, 1086
ApoI RAATTY 2 cut(s) 311, 343
ArsI GACNNNNNNTTYG 4 cut(s) 283, 315, 746, 778
AsiGI ACCGGT 1 cut(s) 756
AspLEI GCGC 2 cut(s) 7, 752
AsuHPI GGTGA 3 cut(s) 512, 721, 1076
AsuII TTCGAA 1 cut(s) 687
AxyI CCTNAGG 1 cut(s) 585
BalI TGGCCA 1 cut(s) 1088
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 183
BarI GAAGNNNNNNTAC 2 cut(s) 1003, 1035
BbsI GAAGAC 1 cut(s) 30
Bbv12I GWGCWC 1 cut(s) 604
BccI CCATC 3 cut(s) 142, 346, 998
BcgI CGANNNNNNTGC 2 cut(s) 366, 400
BciT130I CCWGG 1 cut(s) 51
BclI TGATCA 1 cut(s) 259
BcoDI GTCTC 1 cut(s) 725
BfaI CTAG 2 cut(s) 11, 1044
BfmI CTRYAG 1 cut(s) 1071
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 2 cut(s) 8, 1040
BlsI GCNGC 2 cut(s) 9, 1041
BmcAI AGTACT 1 cut(s) 616
Bme1390I CCNGG 1 cut(s) 51
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 51
BmsI GCATC 3 cut(s) 208, 364, 836
BpiI GAAGAC 1 cut(s) 30
BpmI CTGGAG 1 cut(s) 809
Bpu14I TTCGAA 1 cut(s) 687
BpuEI CTTGAG 3 cut(s) 89, 890, 916
Bsa29I ATCGAT 1 cut(s) 350
BsaBI GATNNNNATC 1 cut(s) 258
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 1089
BsaWI WCCGGW 1 cut(s) 756
Bsc4I CCNNNNNNNGG 1 cut(s) 213
Bse118I RCCGGY 2 cut(s) 756, 844
Bse1I ACTGG 2 cut(s) 792, 845
Bse21I CCTNAGG 1 cut(s) 585
Bse3DI GCAATG 1 cut(s) 1034
Bse8I GATNNNNATC 1 cut(s) 258
BseBI CCWGG 1 cut(s) 51
BseCI ATCGAT 1 cut(s) 350
BseDI CCNNGG 1 cut(s) 1089
BseGI GGATG 6 cut(s) 223, 379, 705, 814, 880, 1009
BseJI GATNNNNATC 1 cut(s) 258
BseLI CCNNNNNNNGG 1 cut(s) 213
BseMI GCAATG 1 cut(s) 1034
BseMII CTCAG 1 cut(s) 158
BseNI ACTGG 2 cut(s) 792, 845
BseRI GAGGAG 1 cut(s) 418
BshFI GGCC 4 cut(s) 530, 583, 844, 1088
BshNI GGYRCC 1 cut(s) 4
BshTI ACCGGT 1 cut(s) 756
BshVI ATCGAT 1 cut(s) 350
BsiHKAI GWGCWC 1 cut(s) 604
BsiSI CCGG 2 cut(s) 757, 845
BslFI GGGAC 1 cut(s) 846
BslI CCNNNNNNNGG 1 cut(s) 213
BsmAI GTCTC 1 cut(s) 725
BsmFI GGGAC 1 cut(s) 846
BsnI GGCC 4 cut(s) 530, 583, 844, 1088
Bsp119I TTCGAA 1 cut(s) 687
Bsp1286I GDGCHC 2 cut(s) 183, 604
Bsp143I GATC 4 cut(s) 259, 619, 736, 1028
BspACI CCGC 3 cut(s) 8, 980, 1040
BspANI GGCC 4 cut(s) 530, 583, 844, 1088
BspCNI CTCAG 1 cut(s) 157
BspDI ATCGAT 1 cut(s) 350
BspHI TCATGA 1 cut(s) 265
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 614
BspT104I TTCGAA 1 cut(s) 687
BspT107I GGYRCC 1 cut(s) 4
BsrDI GCAATG 1 cut(s) 1034
BsrFI RCCGGY 2 cut(s) 756, 844
BsrI ACTGG 2 cut(s) 792, 845
BssAI RCCGGY 2 cut(s) 756, 844
BssECI CCNNGG 1 cut(s) 1089
BssMI GATC 4 cut(s) 259, 619, 736, 1028
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 1089
Bst2UI CCWGG 1 cut(s) 51
Bst4CI ACNGT 2 cut(s) 497, 1064
Bst6I CTCTTC 2 cut(s) 204, 479
BstACI GRCGYC 1 cut(s) 5
BstBI TTCGAA 1 cut(s) 687
BstDEI CTNAG 2 cut(s) 144, 585
BstF5I GGATG 6 cut(s) 223, 379, 705, 814, 880, 1009
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 752
BstKTI GATC 4 cut(s) 262, 622, 739, 1031
BstMAI GTCTC 1 cut(s) 725
BstMBI GATC 4 cut(s) 259, 619, 736, 1028
BstMWI GCNNNNNNNGC 2 cut(s) 89, 589
BstNI CCWGG 1 cut(s) 51
BstNSI RCATGY 1 cut(s) 204
BstSCI CCNGG 1 cut(s) 49
BstSFI CTRYAG 1 cut(s) 1071
BstV2I GAAGAC 1 cut(s) 30
Bsu15I ATCGAT 1 cut(s) 350
Bsu36I CCTNAGG 1 cut(s) 585
BsuRI GGCC 4 cut(s) 530, 583, 844, 1088
BsuTUI ATCGAT 1 cut(s) 350
BtsCI GGATG 6 cut(s) 223, 379, 705, 814, 880, 1009
BtsI GCAGTG 1 cut(s) 902
BtsIMutI CAGTG 1 cut(s) 902
CciI TCATGA 1 cut(s) 265
CfoI GCGC 2 cut(s) 7, 752
Cfr10I RCCGGY 2 cut(s) 756, 844
ClaI ATCGAT 1 cut(s) 350
CsiI ACCWGGT 1 cut(s) 49
Csp6I GTAC 3 cut(s) 162, 318, 615
CspAI ACCGGT 1 cut(s) 756
CviAII CATG 4 cut(s) 201, 266, 305, 904
CviJI RGCY 9 cut(s) 181, 530, 535, 583, 645, 844, 882, 930, 1088
CviKI_1 RGCY 9 cut(s) 181, 530, 535, 583, 645, 844, 882, 930, 1088
CviQI GTAC 3 cut(s) 162, 318, 615
DdeI CTNAG 2 cut(s) 144, 585
DinI GGCGCC 1 cut(s) 6
DpnI GATC 4 cut(s) 261, 621, 738, 1030
DpnII GATC 4 cut(s) 259, 619, 736, 1028
EaeI YGGCCR 2 cut(s) 842, 1086
Eam1104I CTCTTC 2 cut(s) 204, 479
EarI CTCTTC 2 cut(s) 204, 479
Eco130I CCWWGG 1 cut(s) 1089
Eco147I AGGCCT 1 cut(s) 583
Eco24I GRGCYC 1 cut(s) 183
Eco81I CCTNAGG 1 cut(s) 585
EcoRII CCWGG 1 cut(s) 49
EcoT14I CCWWGG 1 cut(s) 1089
EcoT22I ATGCAT 1 cut(s) 379
EcoT38I GRGCYC 1 cut(s) 183
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 1089
FaeI CATG 4 cut(s) 204, 269, 308, 907
FalI AAGNNNNNCTT 2 cut(s) 871, 903
FaqI GGGAC 1 cut(s) 846
FatI CATG 4 cut(s) 200, 265, 304, 903
FauI CCCGC 1 cut(s) 973
FauNDI CATATG 1 cut(s) 594
FbaI TGATCA 1 cut(s) 259
FblI GTMKAC 1 cut(s) 1015
Fnu4HI GCNGC 2 cut(s) 8, 1040
FokI GGATG 6 cut(s) 230, 386, 712, 821, 887, 1016
FriOI GRGCYC 1 cut(s) 183
Fsp4HI GCNGC 2 cut(s) 8, 1040
FspBI CTAG 2 cut(s) 11, 1044
GlaI GCGC 2 cut(s) 6, 751
GluI GCNGC 2 cut(s) 8, 1040
GsuI CTGGAG 1 cut(s) 809
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 4 cut(s) 530, 583, 844, 1088
HapII CCGG 2 cut(s) 757, 845
HhaI GCGC 2 cut(s) 7, 752
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 4 cut(s) 204, 269, 308, 907
Hin6I GCGC 2 cut(s) 5, 750
HinP1I GCGC 2 cut(s) 5, 750
HincII GTYRAC 2 cut(s) 477, 1060
HindII GTYRAC 2 cut(s) 477, 1060
HinfI GANTC 3 cut(s) 241, 449, 573
HpaII CCGG 2 cut(s) 757, 845
HphI GGTGA 3 cut(s) 512, 721, 1076
Hpy166II GTNNAC 3 cut(s) 477, 1016, 1060
Hpy188I TCNGA 7 cut(s) 147, 208, 388, 448, 741, 774, 1028
Hpy188III TCNNGA 6 cut(s) 245, 266, 461, 577, 697, 730
Hpy8I GTNNAC 3 cut(s) 477, 1016, 1060
HpyAV CCTTC 6 cut(s) 112, 520, 550, 635, 665, 1029
HpyCH4III ACNGT 2 cut(s) 497, 1064
HpyCH4IV ACGT 2 cut(s) 390, 924
HpyCH4V TGCA 4 cut(s) 92, 304, 377, 827
HpyF10VI GCNNNNNNNGC 2 cut(s) 89, 589
HpyF3I CTNAG 2 cut(s) 144, 585
HpySE526I ACGT 2 cut(s) 390, 924
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 4 cut(s) 204, 269, 308, 907
HspAI GCGC 2 cut(s) 5, 750
KasI GGCGCC 1 cut(s) 4
Ksp22I TGATCA 1 cut(s) 259
Kzo9I GATC 4 cut(s) 259, 619, 736, 1028
LmnI GCTCC 1 cut(s) 589
LweI GCATC 3 cut(s) 208, 364, 836
MabI ACCWGGT 1 cut(s) 49
MaeI CTAG 2 cut(s) 11, 1044
MaeII ACGT 2 cut(s) 390, 924
MaeIII GTNAC 3 cut(s) 190, 500, 1046
MalI GATC 4 cut(s) 261, 621, 738, 1030
MboI GATC 4 cut(s) 259, 619, 736, 1028
MboII GAAGA 5 cut(s) 35, 221, 496, 559, 667
MfeI CAATTG 1 cut(s) 281
MhlI GDGCHC 2 cut(s) 183, 604
MlsI TGGCCA 1 cut(s) 1088
MluCI AATT 7 cut(s) 184, 281, 294, 311, 343, 430, 625
MluNI TGGCCA 1 cut(s) 1088
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 250
MmeI TCCRAC 1 cut(s) 935
Mox20I TGGCCA 1 cut(s) 1088
Mph1103I ATGCAT 1 cut(s) 379
MscI TGGCCA 1 cut(s) 1088
MseI TTAA 3 cut(s) 132, 174, 552
Msp20I TGGCCA 1 cut(s) 1088
MspI CCGG 2 cut(s) 757, 845
MspR9I CCNGG 1 cut(s) 51
MunI CAATTG 1 cut(s) 281
MvaI CCWGG 1 cut(s) 51
MwoI GCNNNNNNNGC 2 cut(s) 89, 589
NarI GGCGCC 1 cut(s) 5
NdeI CATATG 1 cut(s) 594
NdeII GATC 4 cut(s) 259, 619, 736, 1028
NlaIII CATG 4 cut(s) 204, 269, 308, 907
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 500
NsiI ATGCAT 1 cut(s) 379
NspI RCATGY 1 cut(s) 204
NspV TTCGAA 1 cut(s) 687
PagI TCATGA 1 cut(s) 265
PceI AGGCCT 1 cut(s) 583
PciI ACATGT 1 cut(s) 200
PcsI WCGNNNNNNNCGW 1 cut(s) 501
PfeI GAWTC 2 cut(s) 449, 573
PinAI ACCGGT 1 cut(s) 756
PkrI GCNGC 2 cut(s) 9, 1041
PleI GAGTC 1 cut(s) 249
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 249
PscI ACATGT 1 cut(s) 200
PsiI TTATAA 1 cut(s) 857
Psp1406I AACGTT 1 cut(s) 924
Psp6I CCWGG 1 cut(s) 49
PspGI CCWGG 1 cut(s) 49
PspN4I GGNNCC 1 cut(s) 6
RsaI GTAC 3 cut(s) 163, 319, 616
RsaNI GTAC 3 cut(s) 162, 318, 615
SaqAI TTAA 3 cut(s) 132, 174, 552
SatI GCNGC 2 cut(s) 8, 1040
Sau3AI GATC 4 cut(s) 259, 619, 736, 1028
ScaI AGTACT 1 cut(s) 616
SchI GAGTC 1 cut(s) 250
ScrFI CCNGG 1 cut(s) 51
SduI GDGCHC 2 cut(s) 183, 604
SexAI ACCWGGT 1 cut(s) 49
SfaNI GCATC 3 cut(s) 208, 364, 836
SfcI CTRYAG 1 cut(s) 1071
SfoI GGCGCC 1 cut(s) 6
SfuI TTCGAA 1 cut(s) 687
SmlI CTYRAG 3 cut(s) 104, 869, 931
SmoI CTYRAG 3 cut(s) 104, 869, 931
Sse9I AATT 7 cut(s) 184, 281, 294, 311, 343, 430, 625
SseBI AGGCCT 1 cut(s) 583
SsiI CCGC 3 cut(s) 8, 980, 1040
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 67
SspMI CTAG 2 cut(s) 11, 1044
StuI AGGCCT 1 cut(s) 583
StyD4I CCNGG 1 cut(s) 49
StyI CCWWGG 1 cut(s) 1089
TaaI ACNGT 2 cut(s) 497, 1064
TaiI ACGT 2 cut(s) 393, 927
TaqI TCGA 5 cut(s) 46, 244, 350, 687, 729
TasI AATT 7 cut(s) 184, 281, 294, 311, 343, 430, 625
TatI WGTACW 1 cut(s) 614
TauI GCSGC 2 cut(s) 10, 1042
TfiI GAWTC 2 cut(s) 449, 573
Tru1I TTAA 3 cut(s) 132, 174, 552
Tru9I TTAA 3 cut(s) 132, 174, 552
TscAI CASTG 1 cut(s) 902
TseFI GTSAC 1 cut(s) 500
Tsp45I GTSAC 1 cut(s) 500
TspDTI ATGAA 6 cut(s) 19, 242, 336, 560, 891, 920
TspRI CASTG 1 cut(s) 902
XapI RAATTY 2 cut(s) 311, 343
XceI RCATGY 1 cut(s) 204
XmiI GTMKAC 1 cut(s) 1015
XspI CTAG 2 cut(s) 11, 1044
ZrmI AGTACT 1 cut(s) 616
Zsp2I ATGCAT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.