AT3G24850

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000766
Physical Location & Seq
Forward (+)
9071105 .. 9072184
1080 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G24850.1

Sequence Viewer

Length: 1080 bp
ATGAGTAGTATGTATCACGATTACGATCATCTCACGGCTTCAGGGAAGGGCCTGTGGTCGAAGTTCTATGGACTGGTGGATGCGGCGGTTTTGTTATATGATGAGATACAACGTCGTGGCAAGATCGTATCTGAAGAAGAAAGCCAGAAGAGAATATTTTGTCTCTTCCCGAGAAGAAAAAGATCGTCTTTGGTGAAGAGACAACAAAAGCTTAATGGTGGTTTTATCTCTTTTACTTCTTCATCGCTTATCGATCTTAACCAGTTGCCGACTGATTCTGAGATCGAAGACCCACAAACCTCTGATTCCCAGATGAAAACCCTACAAAACTCTAGTTCTGAACCTTGCACTTCACTCGTACTCTTTGATTACAAAACGGCTGAGTCCGAGAAGATGGAGGCGAAAACCCTACAAAACCCTAGTTCTGAACTTTGTTCATCACTCGTACTCTCTGATTACAAGATGGCTGAGTCGGAAAAGATGGAGACGAAAGACCCACCAAACCCTCTGTCTTTGTGCCTAACGGGGAACACAAGCCGCAAGAGACGTGCTGTGGAAGAGAGGAAACGTACCGGTGGATTCAAGAAGGCAAAGGTTGCTCCTTTTCCAGGGACAGCGAGAGATACGCCTAAGTGGCTTGTCAAGGTGATGAGAGACATGAAAGAAGCCAAAGATGCTAAACTGATCTTTGAGAAGACTCTGTTTGTGACTGATATAAACCCAACACAGAACCGTCTCTCAATGCCGTTCAACAACTTACTCCAAAACGATTTCTTGACGTCTGTGGAGTCCAGAATCATAAAGGAAGACATCAACAACAATAAGAAGATTGGAGTGGGAGCGATTCTCGTGGATCAACGGTCTGTAAAGTGGGGTGTGATGTTGAAAAGATGGGAGCTGAAGAAGGAATCAGGAAAAGGAAGCTGGAACTACAATCTGATTTGTGGGTGGAACGATGTCGTTGAGGCTAACGGATTGAAAGAAGGCGACAACATTAGTGTTTGGTCGTTCAGGTGTCGTGGAGTCCTTTGCTTTGCCATGGAACAGAGTAGCTCTTCTCTTGCTCTCTGTCTCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

359

Amino Acids

40.67

Weight (kDa)

8.98

Isoelectric Point (pI)

62.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 208 - 323 3.6e-46 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 782
AciI CCGC 3 cut(s) 83, 86, 538
AclWI GGATC 1 cut(s) 861
AcuI CTGAAG 3 cut(s) 24, 153, 920
AcyI GRCGYC 1 cut(s) 779
AfaI GTAC 3 cut(s) 360, 447, 571
AfiI CCNNNNNNNGG 1 cut(s) 608
AgeI ACCGGT 1 cut(s) 572
AgsI TTSAA 4 cut(s) 583, 751, 886, 979
AjiI CACGTC 1 cut(s) 548
AjnI CCWGG 1 cut(s) 607
AluBI AGCT 4 cut(s) 211, 898, 924, 1053
AluI AGCT 4 cut(s) 211, 898, 924, 1053
Alw26I GTCTC 7 cut(s) 167, 193, 479, 538, 648, 740, 1076
AlwI GGATC 1 cut(s) 861
Ama87I CYCGRG 1 cut(s) 169
AoxI GGCC 1 cut(s) 49
ArsI GACNNNNNNTTYG 2 cut(s) 494, 526
AsiGI ACCGGT 1 cut(s) 572
AspS9I GGNCC 1 cut(s) 49
AsuHPI GGTGA 2 cut(s) 205, 658
AvaI CYCGRG 1 cut(s) 169
BauI CACGAG 1 cut(s) 848
BbsI GAAGAC 3 cut(s) 294, 701, 813
BccI CCATC 4 cut(s) 388, 457, 475, 885
BceAI ACGGC 3 cut(s) 51, 393, 730
BcgI CGANNNNNNTGC 2 cut(s) 337, 371
BciT130I CCWGG 1 cut(s) 609
BcoDI GTCTC 7 cut(s) 167, 193, 479, 538, 648, 740, 1076
BfaI CTAG 2 cut(s) 333, 420
BglI GCCNNNNNGGC 1 cut(s) 634
BisI GCNGC 2 cut(s) 84, 538
BlsI GCNGC 2 cut(s) 85, 539
Bme1390I CCNGG 1 cut(s) 609
BmeT110I CYCGRG 1 cut(s) 169
BmgBI CACGTC 1 cut(s) 548
BmgT120I GGNCC 1 cut(s) 49
BmrFI CCNGG 1 cut(s) 609
BmsI GCATC 2 cut(s) 70, 664
BpiI GAAGAC 3 cut(s) 294, 701, 813
BplI GAGNNNNNCTC 2 cut(s) 831, 863
Bsa29I ATCGAT 1 cut(s) 252
BsaBI GATNNNNATC 1 cut(s) 24
BsaHI GRCGYC 1 cut(s) 779
BsaJI CCNNGG 2 cut(s) 608, 1038
BsaWI WCCGGW 1 cut(s) 572
Bsc4I CCNNNNNNNGG 1 cut(s) 608
Bse118I RCCGGY 1 cut(s) 572
Bse1I ACTGG 2 cut(s) 78, 262
Bse8I GATNNNNATC 1 cut(s) 24
BseBI CCWGG 1 cut(s) 609
BseCI ATCGAT 1 cut(s) 252
BseDI CCNNGG 2 cut(s) 608, 1038
BseGI GGATG 1 cut(s) 85
BseJI GATNNNNATC 1 cut(s) 24
BseLI CCNNNNNNNGG 1 cut(s) 608
BseMII CTCAG 3 cut(s) 270, 372, 459
BseNI ACTGG 2 cut(s) 78, 262
BshFI GGCC 1 cut(s) 51
BshTI ACCGGT 1 cut(s) 572
BshVI ATCGAT 1 cut(s) 252
BsiHKCI CYCGRG 1 cut(s) 169
BsiSI CCGG 1 cut(s) 573
BslFI GGGAC 1 cut(s) 625
BslI CCNNNNNNNGG 1 cut(s) 608
BsmAI GTCTC 7 cut(s) 167, 193, 479, 538, 648, 740, 1076
BsmBI CGTCTC 3 cut(s) 479, 538, 740
BsmFI GGGAC 1 cut(s) 625
BsnI GGCC 1 cut(s) 51
BsoBI CYCGRG 1 cut(s) 169
Bsp143I GATC 7 cut(s) 25, 123, 182, 253, 282, 684, 853
Bsp19I CCATGG 1 cut(s) 1038
BspACI CCGC 3 cut(s) 83, 86, 538
BspANI GGCC 1 cut(s) 51
BspCNI CTCAG 3 cut(s) 271, 373, 460
BspDI ATCGAT 1 cut(s) 252
BspPI GGATC 1 cut(s) 861
BspQI GCTCTTC 1 cut(s) 1060
BsrFI RCCGGY 1 cut(s) 572
BsrI ACTGG 2 cut(s) 78, 262
BssAI RCCGGY 1 cut(s) 572
BssECI CCNNGG 2 cut(s) 608, 1038
BssMI GATC 7 cut(s) 25, 123, 182, 253, 282, 684, 853
BssNI GRCGYC 1 cut(s) 779
BssSI CACGAG 1 cut(s) 848
BssT1I CCWWGG 1 cut(s) 1038
Bst2BI CACGAG 1 cut(s) 848
Bst2UI CCWGG 1 cut(s) 609
Bst4CI ACNGT 2 cut(s) 734, 861
Bst6I CTCTTC 5 cut(s) 143, 170, 191, 552, 1060
BstACI GRCGYC 1 cut(s) 779
BstAPI GCANNNNNTGC 1 cut(s) 596
BstDEI CTNAG 4 cut(s) 279, 381, 468, 630
BstDSI CCRYGG 1 cut(s) 1038
BstENI CCTNNNNNAGG 1 cut(s) 606
BstF5I GGATG 1 cut(s) 85
BstKTI GATC 7 cut(s) 28, 126, 185, 256, 285, 687, 856
BstMAI GTCTC 7 cut(s) 167, 193, 479, 538, 648, 740, 1076
BstMBI GATC 7 cut(s) 25, 123, 182, 253, 282, 684, 853
BstMWI GCNNNNNNNGC 3 cut(s) 596, 634, 674
BstNI CCWGG 1 cut(s) 609
BstSCI CCNGG 1 cut(s) 607
BstV2I GAAGAC 3 cut(s) 294, 701, 813
Bsu15I ATCGAT 1 cut(s) 252
BsuRI GGCC 1 cut(s) 51
BsuTUI ATCGAT 1 cut(s) 252
BtgI CCRYGG 1 cut(s) 1038
BtgZI GCGATG 1 cut(s) 228
BtrI CACGTC 1 cut(s) 548
BtsCI GGATG 1 cut(s) 85
Cfr10I RCCGGY 1 cut(s) 572
Cfr13I GGNCC 1 cut(s) 49
ClaI ATCGAT 1 cut(s) 252
Csp6I GTAC 3 cut(s) 359, 446, 570
CspAI ACCGGT 1 cut(s) 572
CviAII CATG 2 cut(s) 658, 1039
CviQI GTAC 3 cut(s) 359, 446, 570
DdeI CTNAG 4 cut(s) 279, 381, 468, 630
DpnI GATC 7 cut(s) 27, 125, 184, 255, 284, 686, 855
DpnII GATC 7 cut(s) 25, 123, 182, 253, 282, 684, 853
Eam1104I CTCTTC 5 cut(s) 143, 170, 191, 552, 1060
EarI CTCTTC 5 cut(s) 143, 170, 191, 552, 1060
Eco130I CCWWGG 1 cut(s) 1038
Eco57I CTGAAG 3 cut(s) 24, 153, 920
Eco88I CYCGRG 1 cut(s) 169
EcoNI CCTNNNNNAGG 1 cut(s) 606
EcoO109I RGGNCCY 1 cut(s) 49
EcoRII CCWGG 1 cut(s) 607
EcoT14I CCWWGG 1 cut(s) 1038
ErhI CCWWGG 1 cut(s) 1038
Esp3I CGTCTC 3 cut(s) 479, 538, 740
FaeI CATG 2 cut(s) 661, 1042
FaiI YATR 8 cut(s) 11, 69, 97, 99, 659, 716, 800, 1040
FalI AAGNNNNNCTT 2 cut(s) 172, 204
FaqI GGGAC 1 cut(s) 625
FatI CATG 2 cut(s) 657, 1038
Fnu4HI GCNGC 2 cut(s) 84, 538
FokI GGATG 1 cut(s) 92
Fsp4HI GCNGC 2 cut(s) 84, 538
FspBI CTAG 2 cut(s) 333, 420
GluI GCNGC 2 cut(s) 84, 538
HaeIII GGCC 1 cut(s) 51
HapII CCGG 1 cut(s) 573
Hin1I GRCGYC 1 cut(s) 779
Hin1II CATG 2 cut(s) 661, 1042
HindIII AAGCTT 1 cut(s) 209
HpaII CCGG 1 cut(s) 573
HphI GGTGA 2 cut(s) 205, 658
Hpy188I TCNGA 9 cut(s) 133, 280, 304, 340, 388, 427, 454, 475, 939
Hpy188III TCNNGA 6 cut(s) 17, 169, 583, 775, 792, 912
Hpy99I CGWCG 1 cut(s) 117
HpyAV CCTTC 4 cut(s) 40, 580, 898, 977
HpyCH4III ACNGT 2 cut(s) 734, 861
HpyCH4IV ACGT 4 cut(s) 112, 547, 568, 779
HpyCH4V TGCA 1 cut(s) 348
HpyF10VI GCNNNNNNNGC 3 cut(s) 596, 634, 674
HpyF3I CTNAG 4 cut(s) 279, 381, 468, 630
HpySE526I ACGT 4 cut(s) 112, 547, 568, 779
Hsp92I GRCGYC 1 cut(s) 779
Hsp92II CATG 2 cut(s) 661, 1042
Kzo9I GATC 7 cut(s) 25, 123, 182, 253, 282, 684, 853
LguI GCTCTTC 1 cut(s) 1060
LmnI GCTCC 3 cut(s) 604, 839, 895
LweI GCATC 2 cut(s) 70, 664
MaeI CTAG 2 cut(s) 333, 420
MaeII ACGT 4 cut(s) 112, 547, 568, 779
MaeIII GTNAC 1 cut(s) 706
MalI GATC 7 cut(s) 27, 125, 184, 255, 284, 686, 855
MboI GATC 7 cut(s) 25, 123, 182, 253, 282, 684, 853
MlyI GAGTC 5 cut(s) 392, 479, 691, 797, 1032
MmeI TCCRAC 1 cut(s) 453
MnlI CCTC 5 cut(s) 310, 391, 516, 555, 958
MseI TTAA 2 cut(s) 213, 258
MspI CCGG 1 cut(s) 573
MspR9I CCNGG 1 cut(s) 609
MvaI CCWGG 1 cut(s) 609
MwoI GCNNNNNNNGC 3 cut(s) 596, 634, 674
NcoI CCATGG 1 cut(s) 1038
NdeII GATC 7 cut(s) 25, 123, 182, 253, 282, 684, 853
NlaIII CATG 2 cut(s) 661, 1042
NmuCI GTSAC 1 cut(s) 706
PciSI GCTCTTC 1 cut(s) 1060
PfeI GAWTC 6 cut(s) 275, 305, 579, 795, 844, 908
PinAI ACCGGT 1 cut(s) 572
PkrI GCNGC 2 cut(s) 85, 539
PleI GAGTC 5 cut(s) 391, 478, 691, 796, 1031
PpsI GAGTC 5 cut(s) 391, 478, 691, 796, 1031
Psp6I CCWGG 1 cut(s) 607
PspGI CCWGG 1 cut(s) 607
PspPI GGNCC 1 cut(s) 49
RsaI GTAC 3 cut(s) 360, 447, 571
RsaNI GTAC 3 cut(s) 359, 446, 570
SapI GCTCTTC 1 cut(s) 1060
SaqAI TTAA 2 cut(s) 213, 258
SatI GCNGC 2 cut(s) 84, 538
Sau3AI GATC 7 cut(s) 25, 123, 182, 253, 282, 684, 853
Sau96I GGNCC 1 cut(s) 49
SchI GAGTC 5 cut(s) 392, 479, 691, 797, 1032
ScrFI CCNGG 1 cut(s) 609
SfaNI GCATC 2 cut(s) 70, 664
SsiI CCGC 3 cut(s) 83, 86, 538
SspI AATATT 1 cut(s) 156
SspMI CTAG 2 cut(s) 333, 420
StyD4I CCNGG 1 cut(s) 607
StyI CCWWGG 1 cut(s) 1038
TaaI ACNGT 2 cut(s) 734, 861
TaiI ACGT 4 cut(s) 115, 550, 571, 782
TaqI TCGA 3 cut(s) 59, 252, 285
TauI GCSGC 2 cut(s) 86, 540
TfiI GAWTC 6 cut(s) 275, 305, 579, 795, 844, 908
Tru1I TTAA 2 cut(s) 213, 258
Tru9I TTAA 2 cut(s) 213, 258
TseFI GTSAC 1 cut(s) 706
Tsp45I GTSAC 1 cut(s) 706
TspDTI ATGAA 4 cut(s) 231, 329, 426, 674
TspGWI ACGGA 1 cut(s) 987
XagI CCTNNNNNAGG 1 cut(s) 606
XspI CTAG 2 cut(s) 333, 420
ZraI GACGTC 1 cut(s) 780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.