Rorug01G0425700

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000766
Physical Location & Seq
Forward (+)
52629792 .. 52630521
730 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0425700.1

Sequence Viewer

Length: 600 bp
ATGGGAGCTCAAACTCTCATATCTCATGTCACTCTTCTCCCATTTATCATTATCATCATCATGATAAACATGCACACAACCATGGCAGCCTCCTCTTCAACCCCAGAACCTCACAACTCCTATCTCCTAAGAGCTTGCAATTCAACCACATACCCACAAATTTGCTACAAGTCCCTCTTCTCCTACGCCTCCACAATCAACTCAAACCCTCAGAAGCTCTGCAGCTACGCCCTCTCTGTCGCCCTTAAAGCCGCCCGCAACGCCTCCTCCACCGTGACGAAGCTGTCCAAGAGGAATGGACTGACACACTCAGAGGCTTTGGTCGTCAAGGATTGCATAGAGAATATCAGGGACTCCATTGACGAACTCAAGCAGTCCGTGAGTGCCATGAGCAATTTGGGGGGTGCGAATATGGAATACCAGTTGGGTGACATAAAGACTTGGGTCAGCGCTGCCCTAACAGACGATGATACTTGTATGGAAGGGTTTGATGAGGTGAAGGTGAGCTTGGATGTGAAGAACGCCATCAGTAAGAGCATCGTCAATATTGCTAGATTGGCTAGTAATGCCCTGTCTCTCATCAACAGCCTCAATTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

199

Amino Acids

21.59

Weight (kDa)

6.88

Isoelectric Point (pI)

41.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 44 - 193 1.4e-35 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 283
AciI CCGC 2 cut(s) 252, 256
AcsI RAATTY 1 cut(s) 159
AfeI AGCGCT 1 cut(s) 451
AgsI TTSAA 2 cut(s) 99, 144
AjuI GAANNNNNNNTTGG 2 cut(s) 491, 523
AluBI AGCT 6 cut(s) 8, 134, 217, 225, 283, 507
AluI AGCT 6 cut(s) 8, 134, 217, 225, 283, 507
Alw21I GWGCWC 1 cut(s) 10
Alw26I GTCTC 1 cut(s) 579
Aor51HI AGCGCT 1 cut(s) 451
ApeKI GCWGC 3 cut(s) 86, 222, 452
ApoI RAATTY 1 cut(s) 159
AspLEI GCGC 1 cut(s) 452
AsuHPI GGTGA 3 cut(s) 440, 508, 514
BanII GRGCYC 1 cut(s) 10
Bbv12I GWGCWC 1 cut(s) 10
BbvI GCAGC 3 cut(s) 98, 234, 439
BccI CCATC 1 cut(s) 533
BcoDI GTCTC 1 cut(s) 579
BfaI CTAG 2 cut(s) 552, 561
BfmI CTRYAG 1 cut(s) 220
BfoI RGCGCY 1 cut(s) 453
BisI GCNGC 4 cut(s) 87, 223, 252, 453
BlsI GCNGC 4 cut(s) 88, 224, 253, 454
BmsI GCATC 1 cut(s) 546
BoxI GACNNNNGTC 1 cut(s) 443
BpuEI CTTGAG 1 cut(s) 353
BsaJI CCNNGG 1 cut(s) 81
BsaXI ACNNNNNCTCC 2 cut(s) 251, 281
Bse1I ACTGG 1 cut(s) 421
BseDI CCNNGG 1 cut(s) 81
BseGI GGATG 1 cut(s) 517
BseMII CTCAG 2 cut(s) 224, 324
BseNI ACTGG 1 cut(s) 421
BseRI GAGGAG 2 cut(s) 82, 256
BseXI GCAGC 3 cut(s) 98, 234, 439
BsiHKAI GWGCWC 1 cut(s) 10
BslFI GGGAC 2 cut(s) 157, 365
BsmAI GTCTC 1 cut(s) 579
BsmFI GGGAC 2 cut(s) 157, 365
Bsp1286I GDGCHC 1 cut(s) 10
Bsp19I CCATGG 1 cut(s) 81
BspACI CCGC 2 cut(s) 252, 256
BspCNI CTCAG 2 cut(s) 223, 323
BspHI TCATGA 1 cut(s) 60
BspMAI CTGCAG 1 cut(s) 224
BsrI ACTGG 1 cut(s) 421
BssECI CCNNGG 1 cut(s) 81
BssT1I CCWWGG 1 cut(s) 81
Bst4CI ACNGT 1 cut(s) 274
Bst6I CTCTTC 3 cut(s) 39, 100, 182
BstC8I GCNNGC 2 cut(s) 136, 256
BstDEI CTNAG 3 cut(s) 128, 210, 310
BstDSI CCRYGG 1 cut(s) 81
BstF5I GGATG 1 cut(s) 517
BstH2I RGCGCY 1 cut(s) 453
BstHHI GCGC 1 cut(s) 452
BstMAI GTCTC 1 cut(s) 579
BstMWI GCNNNNNNNGC 4 cut(s) 248, 260, 557, 566
BstNSI RCATGY 1 cut(s) 73
BstPAI GACNNNNGTC 1 cut(s) 443
BstSFI CTRYAG 1 cut(s) 220
BstV1I GCAGC 3 cut(s) 98, 234, 439
BtgI CCRYGG 1 cut(s) 81
BtsCI GGATG 1 cut(s) 517
Cac8I GCNNGC 2 cut(s) 136, 256
CciI TCATGA 1 cut(s) 60
CfoI GCGC 1 cut(s) 452
CviAII CATG 5 cut(s) 26, 61, 70, 82, 388
DdeI CTNAG 3 cut(s) 128, 210, 310
DrdI GACNNNNNNGTC 1 cut(s) 283
DseDI GACNNNNNNGTC 1 cut(s) 283
Eam1104I CTCTTC 3 cut(s) 39, 100, 182
EarI CTCTTC 3 cut(s) 39, 100, 182
Ecl136II GAGCTC 1 cut(s) 8
Eco130I CCWWGG 1 cut(s) 81
Eco24I GRGCYC 1 cut(s) 10
Eco47III AGCGCT 1 cut(s) 451
Eco53kI GAGCTC 1 cut(s) 8
EcoICRI GAGCTC 1 cut(s) 8
EcoT14I CCWWGG 1 cut(s) 81
EcoT38I GRGCYC 1 cut(s) 10
ErhI CCWWGG 1 cut(s) 81
FaeI CATG 5 cut(s) 29, 64, 73, 85, 391
FalI AAGNNNNNCTT 4 cut(s) 161, 193, 491, 523
FaqI GGGAC 2 cut(s) 157, 365
FatI CATG 5 cut(s) 25, 60, 69, 81, 387
FauI CCCGC 1 cut(s) 263
Fnu4HI GCNGC 4 cut(s) 87, 223, 252, 453
FokI GGATG 1 cut(s) 524
FriOI GRGCYC 1 cut(s) 10
Fsp4HI GCNGC 4 cut(s) 87, 223, 252, 453
FspBI CTAG 2 cut(s) 552, 561
GlaI GCGC 1 cut(s) 451
GluI GCNGC 4 cut(s) 87, 223, 252, 453
HaeII RGCGCY 1 cut(s) 453
HhaI GCGC 1 cut(s) 452
Hin1II CATG 5 cut(s) 29, 64, 73, 85, 391
Hin6I GCGC 1 cut(s) 450
HinP1I GCGC 1 cut(s) 450
HinfI GANTC 1 cut(s) 353
HphI GGTGA 3 cut(s) 440, 508, 514
Hpy188I TCNGA 2 cut(s) 213, 313
Hpy188III TCNNGA 1 cut(s) 61
HpyAV CCTTC 2 cut(s) 476, 493
HpyCH4III ACNGT 1 cut(s) 274
HpyCH4V TGCA 4 cut(s) 73, 138, 222, 336
HpyF10VI GCNNNNNNNGC 4 cut(s) 248, 260, 557, 566
HpyF3I CTNAG 3 cut(s) 128, 210, 310
Hsp92II CATG 5 cut(s) 29, 64, 73, 85, 391
HspAI GCGC 1 cut(s) 450
LmnI GCTCC 1 cut(s) 5
LpnPI CCDG 4 cut(s) 117, 334, 434, 584
Lsp1109I GCAGC 3 cut(s) 98, 234, 439
LweI GCATC 1 cut(s) 546
MaeI CTAG 2 cut(s) 552, 561
MaeIII GTNAC 3 cut(s) 28, 274, 428
MboII GAAGA 4 cut(s) 26, 87, 169, 529
MhlI GDGCHC 1 cut(s) 10
MluCI AATT 4 cut(s) 139, 159, 394, 592
MlyI GAGTC 1 cut(s) 347
MseI TTAA 1 cut(s) 246
MslI CAYNNNNRTG 2 cut(s) 59, 80
MwoI GCNNNNNNNGC 4 cut(s) 248, 260, 557, 566
NcoI CCATGG 1 cut(s) 81
NlaIII CATG 5 cut(s) 29, 64, 73, 85, 391
NmuCI GTSAC 3 cut(s) 28, 274, 428
NspI RCATGY 1 cut(s) 73
PagI TCATGA 1 cut(s) 60
PkrI GCNGC 4 cut(s) 88, 224, 253, 454
PleI GAGTC 1 cut(s) 347
PpsI GAGTC 1 cut(s) 347
PshAI GACNNNNGTC 1 cut(s) 443
Psp124BI GAGCTC 1 cut(s) 10
PstI CTGCAG 1 cut(s) 224
RseI CAYNNNNRTG 2 cut(s) 59, 80
SacI GAGCTC 1 cut(s) 10
SaqAI TTAA 1 cut(s) 246
SatI GCNGC 4 cut(s) 87, 223, 252, 453
SchI GAGTC 1 cut(s) 347
SduI GDGCHC 1 cut(s) 10
SetI ASST 9 cut(s) 10, 112, 136, 219, 227, 285, 498, 504, 509
SfaNI GCATC 1 cut(s) 546
SfcI CTRYAG 1 cut(s) 220
SmiMI CAYNNNNRTG 2 cut(s) 59, 80
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
Sse9I AATT 4 cut(s) 139, 159, 394, 592
SsiI CCGC 2 cut(s) 252, 256
SspI AATATT 1 cut(s) 547
SspMI CTAG 2 cut(s) 552, 561
SstI GAGCTC 1 cut(s) 10
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 1 cut(s) 274
TasI AATT 4 cut(s) 139, 159, 394, 592
TauI GCSGC 1 cut(s) 254
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TseFI GTSAC 3 cut(s) 28, 274, 428
TseI GCWGC 3 cut(s) 86, 222, 452
Tsp45I GTSAC 3 cut(s) 28, 274, 428
TspGWI ACGGA 1 cut(s) 367
XapI RAATTY 1 cut(s) 159
XceI RCATGY 1 cut(s) 73
XcmI CCANNNNNNNNNTGG 1 cut(s) 394
XspI CTAG 2 cut(s) 552, 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.