Belongs to the cystatin family. Phytocystatin subfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 122 |
| AccII | CGCG | 2 cut(s) | 81, 146 |
| AciI | CCGC | 5 cut(s) | 71, 79, 146, 204, 251 |
| AclI | AACGTT | 1 cut(s) | 108 |
| AcsI | RAATTY | 2 cut(s) | 140, 317 |
| AfaI | GTAC | 2 cut(s) | 158, 229 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 244 |
| AflII | CTTAAG | 1 cut(s) | 242 |
| AhlI | ACTAGT | 1 cut(s) | 5 |
| Alw26I | GTCTC | 3 cut(s) | 154, 185, 203 |
| ApeKI | GCWGC | 1 cut(s) | 74 |
| ApoI | RAATTY | 2 cut(s) | 140, 317 |
| AspS9I | GGNCC | 1 cut(s) | 115 |
| AsuHPI | GGTGA | 1 cut(s) | 149 |
| AvaII | GGWCC | 1 cut(s) | 115 |
| BbsI | GAAGAC | 1 cut(s) | 10 |
| BbvI | GCAGC | 1 cut(s) | 61 |
| BccI | CCATC | 1 cut(s) | 107 |
| BceAI | ACGGC | 2 cut(s) | 277, 292 |
| BcoDI | GTCTC | 3 cut(s) | 154, 185, 203 |
| BcuI | ACTAGT | 1 cut(s) | 5 |
| BfaI | CTAG | 1 cut(s) | 6 |
| BfrI | CTTAAG | 1 cut(s) | 242 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 71 |
| BisI | GCNGC | 4 cut(s) | 72, 75, 205, 252 |
| BlsI | GCNGC | 4 cut(s) | 73, 76, 206, 253 |
| Bme18I | GGWCC | 1 cut(s) | 115 |
| BmgT120I | GGNCC | 1 cut(s) | 115 |
| BmiI | GGNNCC | 1 cut(s) | 117 |
| BoxI | GACNNNNGTC | 1 cut(s) | 215 |
| BpiI | GAAGAC | 1 cut(s) | 10 |
| BpuEI | CTTGAG | 2 cut(s) | 105, 167 |
| BsaI | GGTCTC | 1 cut(s) | 154 |
| BsaJI | CCNNGG | 1 cut(s) | 308 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 244 |
| BseDI | CCNNGG | 1 cut(s) | 308 |
| BseGI | GGATG | 1 cut(s) | 318 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 244 |
| BseMII | CTCAG | 1 cut(s) | 227 |
| BseRI | GAGGAG | 2 cut(s) | 20, 41 |
| BseXI | GCAGC | 1 cut(s) | 61 |
| Bsh1236I | CGCG | 2 cut(s) | 81, 146 |
| BslFI | GGGAC | 3 cut(s) | 81, 192, 314 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 244 |
| BsmAI | GTCTC | 3 cut(s) | 154, 185, 203 |
| BsmBI | CGTCTC | 1 cut(s) | 185 |
| BsmFI | GGGAC | 3 cut(s) | 81, 192, 314 |
| Bso31I | GGTCTC | 1 cut(s) | 154 |
| Bsp143I | GATC | 1 cut(s) | 132 |
| BspACI | CCGC | 5 cut(s) | 71, 79, 146, 204, 251 |
| BspCNI | CTCAG | 1 cut(s) | 226 |
| BspFNI | CGCG | 2 cut(s) | 81, 146 |
| BspLI | GGNNCC | 1 cut(s) | 117 |
| BspTI | CTTAAG | 1 cut(s) | 242 |
| BspTNI | GGTCTC | 1 cut(s) | 154 |
| BssECI | CCNNGG | 1 cut(s) | 308 |
| BssMI | GATC | 1 cut(s) | 132 |
| Bst4CI | ACNGT | 2 cut(s) | 196, 239 |
| BstAFI | CTTAAG | 1 cut(s) | 242 |
| BstC8I | GCNNGC | 2 cut(s) | 79, 345 |
| BstDEI | CTNAG | 1 cut(s) | 213 |
| BstDSI | CCRYGG | 1 cut(s) | 308 |
| BstF5I | GGATG | 1 cut(s) | 318 |
| BstFNI | CGCG | 2 cut(s) | 81, 146 |
| BstKTI | GATC | 1 cut(s) | 135 |
| BstMAI | GTCTC | 3 cut(s) | 154, 185, 203 |
| BstMBI | GATC | 1 cut(s) | 132 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 71 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 215 |
| BstUI | CGCG | 2 cut(s) | 81, 146 |
| BstV1I | GCAGC | 1 cut(s) | 61 |
| BstV2I | GAAGAC | 1 cut(s) | 10 |
| BtgI | CCRYGG | 1 cut(s) | 308 |
| BtsCI | GGATG | 1 cut(s) | 318 |
| Cac8I | GCNNGC | 2 cut(s) | 79, 345 |
| Cfr13I | GGNCC | 1 cut(s) | 115 |
| CseI | GACGC | 1 cut(s) | 70 |
| Csp6I | GTAC | 2 cut(s) | 157, 228 |
| CviJI | RGCY | 4 cut(s) | 74, 254, 307, 343 |
| CviKI_1 | RGCY | 4 cut(s) | 74, 254, 307, 343 |
| CviQI | GTAC | 2 cut(s) | 157, 228 |
| DdeI | CTNAG | 1 cut(s) | 213 |
| DpnI | GATC | 1 cut(s) | 134 |
| DpnII | GATC | 1 cut(s) | 132 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 122 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 122 |
| Eco31I | GGTCTC | 1 cut(s) | 154 |
| Eco47I | GGWCC | 1 cut(s) | 115 |
| EcoRI | GAATTC | 1 cut(s) | 140 |
| Esp3I | CGTCTC | 1 cut(s) | 185 |
| FaiI | YATR | 1 cut(s) | 298 |
| FalI | AAGNNNNNCTT | 3 cut(s) | 34, 269, 301 |
| FaqI | GGGAC | 3 cut(s) | 81, 192, 314 |
| FauI | CCCGC | 1 cut(s) | 86 |
| Fnu4HI | GCNGC | 4 cut(s) | 72, 75, 205, 252 |
| FokI | GGATG | 1 cut(s) | 325 |
| Fsp4HI | GCNGC | 4 cut(s) | 72, 75, 205, 252 |
| FspBI | CTAG | 1 cut(s) | 6 |
| GluI | GCNGC | 4 cut(s) | 72, 75, 205, 252 |
| HgaI | GACGC | 1 cut(s) | 70 |
| HinfI | GANTC | 4 cut(s) | 94, 173, 180, 211 |
| HphI | GGTGA | 1 cut(s) | 149 |
| Hpy188I | TCNGA | 2 cut(s) | 154, 323 |
| Hpy188III | TCNNGA | 2 cut(s) | 177, 190 |
| Hpy99I | CGWCG | 4 cut(s) | 44, 47, 86, 269 |
| HpyAV | CCTTC | 2 cut(s) | 32, 346 |
| HpyCH4III | ACNGT | 2 cut(s) | 196, 239 |
| HpyCH4IV | ACGT | 1 cut(s) | 108 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 71 |
| HpyF3I | CTNAG | 1 cut(s) | 213 |
| HpySE526I | ACGT | 1 cut(s) | 108 |
| Kzo9I | GATC | 1 cut(s) | 132 |
| LmnI | GCTCC | 1 cut(s) | 168 |
| LpnPI | CCDG | 3 cut(s) | 200, 210, 357 |
| Lsp1109I | GCAGC | 1 cut(s) | 61 |
| MaeI | CTAG | 1 cut(s) | 6 |
| MaeII | ACGT | 1 cut(s) | 108 |
| MaeIII | GTNAC | 1 cut(s) | 109 |
| MalI | GATC | 1 cut(s) | 134 |
| MboI | GATC | 1 cut(s) | 132 |
| MboII | GAAGA | 2 cut(s) | 10, 17 |
| MluCI | AATT | 3 cut(s) | 140, 232, 317 |
| MlyI | GAGTC | 3 cut(s) | 103, 174, 205 |
| MmeI | TCCRAC | 1 cut(s) | 71 |
| MnlI | CCTC | 6 cut(s) | 41, 59, 62, 66, 76, 129 |
| MseI | TTAA | 2 cut(s) | 243, 367 |
| MspCI | CTTAAG | 1 cut(s) | 242 |
| MvnI | CGCG | 2 cut(s) | 81, 146 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 71 |
| NdeII | GATC | 1 cut(s) | 132 |
| NlaIV | GGNNCC | 1 cut(s) | 117 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 150 |
| PfeI | GAWTC | 1 cut(s) | 173 |
| PkrI | GCNGC | 4 cut(s) | 73, 76, 206, 253 |
| PleI | GAGTC | 3 cut(s) | 102, 174, 205 |
| PpsI | GAGTC | 3 cut(s) | 102, 174, 205 |
| PshAI | GACNNNNGTC | 1 cut(s) | 215 |
| Psp1406I | AACGTT | 1 cut(s) | 108 |
| PspN4I | GGNNCC | 1 cut(s) | 117 |
| PspPI | GGNCC | 1 cut(s) | 115 |
| RsaI | GTAC | 2 cut(s) | 158, 229 |
| RsaNI | GTAC | 2 cut(s) | 157, 228 |
| SaqAI | TTAA | 2 cut(s) | 243, 367 |
| SatI | GCNGC | 4 cut(s) | 72, 75, 205, 252 |
| Sau3AI | GATC | 1 cut(s) | 132 |
| Sau96I | GGNCC | 1 cut(s) | 115 |
| SchI | GAGTC | 3 cut(s) | 103, 174, 205 |
| SetI | ASST | 4 cut(s) | 15, 111, 219, 249 |
| SinI | GGWCC | 1 cut(s) | 115 |
| SmlI | CTYRAG | 3 cut(s) | 120, 182, 242 |
| SmoI | CTYRAG | 3 cut(s) | 120, 182, 242 |
| SpeI | ACTAGT | 1 cut(s) | 5 |
| Sse9I | AATT | 3 cut(s) | 140, 232, 317 |
| SsiI | CCGC | 5 cut(s) | 71, 79, 146, 204, 251 |
| SspMI | CTAG | 1 cut(s) | 6 |
| TaaI | ACNGT | 2 cut(s) | 196, 239 |
| TaiI | ACGT | 1 cut(s) | 111 |
| TaqI | TCGA | 2 cut(s) | 189, 339 |
| TasI | AATT | 3 cut(s) | 140, 232, 317 |
| TatI | WGTACW | 1 cut(s) | 156 |
| TauI | GCSGC | 3 cut(s) | 74, 207, 254 |
| TfiI | GAWTC | 1 cut(s) | 173 |
| Tru1I | TTAA | 2 cut(s) | 243, 367 |
| Tru9I | TTAA | 2 cut(s) | 243, 367 |
| TseI | GCWGC | 1 cut(s) | 74 |
| TspDTI | ATGAA | 1 cut(s) | 329 |
| TspGWI | ACGGA | 3 cut(s) | 128, 181, 282 |
| Vha464I | CTTAAG | 1 cut(s) | 242 |
| VpaK11BI | GGWCC | 1 cut(s) | 115 |
| XapI | RAATTY | 2 cut(s) | 140, 317 |
| XspI | CTAG | 1 cut(s) | 6 |