Belongs to the cystatin family. Phytocystatin subfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 74 |
| AccB1I | GGYRCC | 1 cut(s) | 9 |
| AclWI | GGATC | 1 cut(s) | 113 |
| AfaI | GTAC | 1 cut(s) | 161 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 49, 130 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 167, 199 |
| Alw26I | GTCTC | 2 cut(s) | 91, 206 |
| AlwI | GGATC | 1 cut(s) | 113 |
| AoxI | GGCC | 1 cut(s) | 57 |
| AspS9I | GGNCC | 1 cut(s) | 202 |
| AsuC2I | CCSGG | 1 cut(s) | 206 |
| AsuHPI | GGTGA | 1 cut(s) | 128 |
| AvaII | GGWCC | 1 cut(s) | 202 |
| BanI | GGYRCC | 1 cut(s) | 9 |
| BccI | CCATC | 1 cut(s) | 303 |
| BceAI | ACGGC | 1 cut(s) | 134 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 62, 96 |
| BcnI | CCSGG | 1 cut(s) | 206 |
| BcoDI | GTCTC | 2 cut(s) | 91, 206 |
| Bme1390I | CCNGG | 1 cut(s) | 206 |
| Bme18I | GGWCC | 1 cut(s) | 202 |
| BmgT120I | GGNCC | 1 cut(s) | 202 |
| BmiI | GGNNCC | 1 cut(s) | 11 |
| BmrFI | CCNGG | 1 cut(s) | 206 |
| BmsI | GCATC | 1 cut(s) | 240 |
| BoxI | GACNNNNGTC | 1 cut(s) | 218 |
| BpuMI | CCSGG | 1 cut(s) | 206 |
| BsaI | GGTCTC | 1 cut(s) | 91 |
| BsaJI | CCNNGG | 1 cut(s) | 205 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 49, 130 |
| Bse3DI | GCAATG | 1 cut(s) | 78 |
| BseDI | CCNNGG | 1 cut(s) | 205 |
| BseGI | GGATG | 1 cut(s) | 318 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 49, 130 |
| BseMI | GCAATG | 1 cut(s) | 78 |
| BseMII | CTCAG | 1 cut(s) | 230 |
| BshFI | GGCC | 1 cut(s) | 59 |
| BshNI | GGYRCC | 1 cut(s) | 9 |
| BsiSI | CCGG | 1 cut(s) | 205 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 49, 130 |
| BsmAI | GTCTC | 2 cut(s) | 91, 206 |
| BsnI | GGCC | 1 cut(s) | 59 |
| Bso31I | GGTCTC | 1 cut(s) | 91 |
| Bsp143I | GATC | 2 cut(s) | 118, 330 |
| BspANI | GGCC | 1 cut(s) | 59 |
| BspCNI | CTCAG | 1 cut(s) | 229 |
| BspLI | GGNNCC | 1 cut(s) | 11 |
| BspPI | GGATC | 1 cut(s) | 113 |
| BspT107I | GGYRCC | 1 cut(s) | 9 |
| BspTNI | GGTCTC | 1 cut(s) | 91 |
| BsrDI | GCAATG | 1 cut(s) | 78 |
| BssECI | CCNNGG | 1 cut(s) | 205 |
| BssMI | GATC | 2 cut(s) | 118, 330 |
| Bst6I | CTCTTC | 2 cut(s) | 44, 299 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 32 |
| BstC8I | GCNNGC | 1 cut(s) | 229 |
| BstDEI | CTNAG | 1 cut(s) | 216 |
| BstF5I | GGATG | 1 cut(s) | 318 |
| BstKTI | GATC | 2 cut(s) | 121, 333 |
| BstMAI | GTCTC | 2 cut(s) | 91, 206 |
| BstMBI | GATC | 2 cut(s) | 118, 330 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 32, 146 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 218 |
| BstSCI | CCNGG | 1 cut(s) | 204 |
| BsuRI | GGCC | 1 cut(s) | 59 |
| BtsCI | GGATG | 1 cut(s) | 318 |
| Cac8I | GCNNGC | 1 cut(s) | 229 |
| Cfr13I | GGNCC | 1 cut(s) | 202 |
| Csp6I | GTAC | 1 cut(s) | 160 |
| CviAII | CATG | 1 cut(s) | 125 |
| CviJI | RGCY | 3 cut(s) | 59, 244, 290 |
| CviKI_1 | RGCY | 3 cut(s) | 59, 244, 290 |
| CviQI | GTAC | 1 cut(s) | 160 |
| DdeI | CTNAG | 1 cut(s) | 216 |
| DpnI | GATC | 2 cut(s) | 120, 332 |
| DpnII | GATC | 2 cut(s) | 118, 330 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 74 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 74 |
| Eam1104I | CTCTTC | 2 cut(s) | 44, 299 |
| EarI | CTCTTC | 2 cut(s) | 44, 299 |
| Eco147I | AGGCCT | 1 cut(s) | 59 |
| Eco31I | GGTCTC | 1 cut(s) | 91 |
| Eco47I | GGWCC | 1 cut(s) | 202 |
| FaeI | CATG | 1 cut(s) | 128 |
| FaiI | YATR | 4 cut(s) | 126, 251, 285, 298 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 167, 199 |
| FatI | CATG | 1 cut(s) | 124 |
| FokI | GGATG | 1 cut(s) | 325 |
| HaeIII | GGCC | 1 cut(s) | 59 |
| HapII | CCGG | 1 cut(s) | 205 |
| Hin1II | CATG | 1 cut(s) | 128 |
| HinfI | GANTC | 2 cut(s) | 214, 269 |
| HpaII | CCGG | 1 cut(s) | 205 |
| HphI | GGTGA | 1 cut(s) | 128 |
| Hpy188I | TCNGA | 2 cut(s) | 157, 195 |
| Hpy188III | TCNNGA | 2 cut(s) | 277, 322 |
| HpyAV | CCTTC | 4 cut(s) | 53, 70, 124, 283 |
| HpyCH4V | TGCA | 2 cut(s) | 83, 140 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 32, 146 |
| HpyF3I | CTNAG | 1 cut(s) | 216 |
| Hsp92II | CATG | 1 cut(s) | 128 |
| Kzo9I | GATC | 2 cut(s) | 118, 330 |
| LpnPI | CCDG | 6 cut(s) | 35, 185, 203, 213, 218, 307 |
| LweI | GCATC | 1 cut(s) | 240 |
| MalI | GATC | 2 cut(s) | 120, 332 |
| MboI | GATC | 2 cut(s) | 118, 330 |
| MboII | GAAGA | 5 cut(s) | 31, 187, 190, 275, 316 |
| MluCI | AATT | 3 cut(s) | 280, 326, 352 |
| MlyI | GAGTC | 1 cut(s) | 208 |
| MmeI | TCCRAC | 2 cut(s) | 74, 135 |
| MnlI | CCTC | 4 cut(s) | 32, 57, 67, 280 |
| MseI | TTAA | 1 cut(s) | 355 |
| MspI | CCGG | 1 cut(s) | 205 |
| MspR9I | CCNGG | 1 cut(s) | 206 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 32, 146 |
| NciI | CCSGG | 1 cut(s) | 206 |
| NdeII | GATC | 2 cut(s) | 118, 330 |
| NlaIII | CATG | 1 cut(s) | 128 |
| NlaIV | GGNNCC | 1 cut(s) | 11 |
| PceI | AGGCCT | 1 cut(s) | 59 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 69 |
| PfeI | GAWTC | 1 cut(s) | 269 |
| PleI | GAGTC | 1 cut(s) | 208 |
| PpsI | GAGTC | 1 cut(s) | 208 |
| PshAI | GACNNNNGTC | 1 cut(s) | 218 |
| PspN4I | GGNNCC | 1 cut(s) | 11 |
| PspPI | GGNCC | 1 cut(s) | 202 |
| RsaI | GTAC | 1 cut(s) | 161 |
| RsaNI | GTAC | 1 cut(s) | 160 |
| SaqAI | TTAA | 1 cut(s) | 355 |
| Sau3AI | GATC | 2 cut(s) | 118, 330 |
| Sau96I | GGNCC | 1 cut(s) | 202 |
| SchI | GAGTC | 1 cut(s) | 208 |
| ScrFI | CCNGG | 1 cut(s) | 206 |
| SetI | ASST | 3 cut(s) | 18, 78, 222 |
| SfaNI | GCATC | 1 cut(s) | 240 |
| SinI | GGWCC | 1 cut(s) | 202 |
| Sse9I | AATT | 3 cut(s) | 280, 326, 352 |
| SseBI | AGGCCT | 1 cut(s) | 59 |
| StuI | AGGCCT | 1 cut(s) | 59 |
| StyD4I | CCNGG | 1 cut(s) | 204 |
| TaqI | TCGA | 1 cut(s) | 276 |
| TasI | AATT | 3 cut(s) | 280, 326, 352 |
| TatI | WGTACW | 1 cut(s) | 159 |
| TfiI | GAWTC | 1 cut(s) | 269 |
| Tru1I | TTAA | 1 cut(s) | 355 |
| Tru9I | TTAA | 1 cut(s) | 355 |
| TspDTI | ATGAA | 2 cut(s) | 329, 357 |
| VpaK11BI | GGWCC | 1 cut(s) | 202 |