Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000425

Physical Location & Seq

Genomic Coordinates Forward (+)

47395215 .. 47395601

Gene Structure 387 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1358900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 387 bp

ATGCGCCCTCACTGCCTCCTCATCCTCGCCCTCGTCCTCCTTCCTCTGGTGGCCGCTGCCGCGGACCGACGGGGCCTTATAACCGGCGGTTGGGCGCCCATAAAGAACATCAGCGACCCCCATGTGAAAGAGATTGCAGAGTTTGCGGTGTCGGAGTACAGCAAGCAAGCCCAAGGCCAGAACAAGTTGGCGTTTGAGAGAGTCGTCCGGGGCGACAGCCAGGTGGTGGCGGGCATCAACTACCGGCTTGTCATTTCCGCTAAGAACAAGTCCGTGGCTGATCCCGATGTTGCCACTCCCGCGGATTACGAGGGTGTTGTGTGGGAGAAGGCTTGGGAGCATTTTAAGCAGTTGATATCATTTCATCGATTGTCAAAGCCTAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002020 GO:0003674 GO:0004857 GO:0004866 GO:0004869 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005623 GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0009892 GO:0009987 GO:0010029 GO:0010030 GO:0010466 GO:0010605 GO:0010951 GO:0019222 GO:0019899 GO:0030162 GO:0030234 GO:0030312 GO:0030414 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0033554 GO:0034605 GO:0043086 GO:0044092 GO:0044464 GO:0045861 GO:0048518 GO:0048519 GO:0048523 GO:0048580 GO:0048582 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0051716 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0065007 GO:0065009 GO:0071944 GO:0080090 GO:0098772 GO:1900140 GO:2000026 GO:2000116 GO:2000117

KEGG Pathways

Metabolic & Signaling

ko04970 map04970

Pfam Domains

Protein Families

SQAPI

Protein Analysis

129

Amino Acids

14.16

Weight (kDa)

9.13

Isoelectric Point (pI)

27.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cystatin	PF00031	29 - 119	1.5e-13	Cystatin domain
SQAPI	PF16845	34 - 123	1.9e-30	Aspartic acid proteinase inhibitor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000425)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G47550
fragaria_vesca	FvH4_3g00830 FvH4_3g07950 FvH4_3g08020 FvH4_3g34980 FvH4_6g03900 FvH4_6g08240
malus_domestica	MD03G1148300.v1.1 MD04G1217400.v1.1 MD05G1358700.v1.1 MD05G1358900.v1.1 MD05G1359200.v1.1 MD10G1333500.v1.1 MD12G1232100.v1.1
prunus_persica	Prupe.4G008900_v2.0.a1 Prupe.4G009000_v2.0.a1 Prupe.4G009100_v2.0.a1 Prupe.6G215700_v2.0.a1 Prupe.6G335600_v2.0.a1 Prupe.6G335700_v2.0.a1
pyrus_communis	pycom04g19190 pycom05g32440 pycom12g21450 pycom12g21470
rosa_chinensis	RchiOBHm_Chr1g0361291 RchiOBHm_Chr3g0452681 RchiOBHm_Chr3g0455001 RchiOBHm_Chr5g0001051 RchiOBHm_Chr5g0001151 RchiOBHm_Chr5g0010271 RchiOBHm_Chr5g0010621
rosa_laevigata	RLG00000025391 RLG00000025394 RLG00000027728 RLG00000030917 RLG00000031733 RLG00000031766
rosa_multiflora	Rmu_co8350815.1_g000001 Rmu_co8502581.1_g000001 Rmu_sc0000255.1_g000041 Rmu_sc0000818.1_g000015 Rmu_sc0001706.1_g000018 Rmu_sc0001706.1_g000034 Rmu_sc0002150.1_g000001 Rmu_sc0004189.1_g000004 Rmu_sc0014635.1_g000005 Rmu_sc0016264.1_g000005 Rmu_sc0016264.1_g000006 Rmu_sc0021253.1_g000005 Rmu_ssc0000156.1_g000008
rosa_roxburghii	Rroxscaffold_1G00065750 Rroxscaffold_1G00065970 Rroxscaffold_1G00075200 Rroxscaffold_1G00075240 Rroxscaffold_4G00295080 Rroxscaffold_6G00412050 Rroxscaffold_6G00424610
rosa_rugosa	Rorug01G0287800 Rorug02G0642700 Rorug03G0004000 Rorug04G0389300 Rorug04G0449900 Rorug04G0450000 Rorug04G0451200 Rorug05G0127000
rosa_samantha	Rh1AG227300 Rh1AG299200 Rh1BG263000 Rh1CG280500 Rh1DG293000 Rh3AG045000 Rh3AG063500 Rh3BG047300 Rh3BG065400 Rh3CG045800 Rh3CG064300 Rh3DG065500 Rh4CG037800 Rh5AG080900 Rh5BG010600 Rh5BG011000 Rh5BG011100 Rh5BG075500 Rh5CG009200 Rh5CG009700 Rh5CG089100 Rh5CG090700 Rh5DG078200
rosa_wichuraiana	Rw0G000700 Rw0G021870 Rw1G026470 Rw2G026200 Rw3G004990 Rw4G002670 Rw5G000930 Rw5G007490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	80
AccB1I	GGYRCC	1 cut(s)	94
AccB7I	CCANNNNNTGG	1 cut(s)	226
AccII	CGCG	2 cut(s)	62, 302
AciI	CCGC	9 cut(s)	54, 60, 62, 87, 146, 230, 258, 300, 302
AclWI	GGATC	1 cut(s)	275
AcoI	YGGCCR	1 cut(s)	51
AcyI	GRCGYC	1 cut(s)	95
AfaI	GTAC	1 cut(s)	158
AfiI	CCNNNNNNNGG	3 cut(s)	46, 90, 226
AjnI	CCWGG	1 cut(s)	219
AlwI	GGATC	1 cut(s)	275
AoxI	GGCC	3 cut(s)	51, 73, 175
ApeKI	GCWGC	1 cut(s)	56
AspLEI	GCGC	2 cut(s)	6, 97
AspS9I	GGNCC	2 cut(s)	64, 73
AsuC2I	CCSGG	1 cut(s)	209
AvaII	GGWCC	1 cut(s)	64
BanI	GGYRCC	1 cut(s)	94
BbvI	GCAGC	1 cut(s)	43
BciT130I	CCWGG	1 cut(s)	221
BcnI	CCSGG	1 cut(s)	209
BfaI	CTAG	1 cut(s)	385
BfoI	RGCGCY	1 cut(s)	98
BisI	GCNGC	3 cut(s)	54, 57, 60
BlsI	GCNGC	3 cut(s)	55, 58, 61
Bme1390I	CCNGG	2 cut(s)	209, 221
Bme18I	GGWCC	1 cut(s)	64
BmgT120I	GGNCC	2 cut(s)	64, 73
BmiI	GGNNCC	2 cut(s)	74, 96
BmrFI	CCNGG	2 cut(s)	209, 221
BmsI	GCATC	1 cut(s)	243
BpuMI	CCSGG	1 cut(s)	209
Bsa29I	ATCGAT	1 cut(s)	367
BsaHI	GRCGYC	1 cut(s)	95
BsaJI	CCNNGG	5 cut(s)	60, 172, 208, 273, 300
Bsc4I	CCNNNNNNNGG	3 cut(s)	46, 90, 226
Bse118I	RCCGGY	2 cut(s)	83, 243
BseBI	CCWGG	1 cut(s)	221
BseCI	ATCGAT	1 cut(s)	367
BseDI	CCNNGG	5 cut(s)	60, 172, 208, 273, 300
BseGI	GGATG	1 cut(s)	21
BseLI	CCNNNNNNNGG	3 cut(s)	46, 90, 226
BseRI	GAGGAG	1 cut(s)	8
BseXI	GCAGC	1 cut(s)	43
Bsh1236I	CGCG	2 cut(s)	62, 302
BshFI	GGCC	3 cut(s)	53, 75, 177
BshNI	GGYRCC	1 cut(s)	94
BshVI	ATCGAT	1 cut(s)	367
BsiSI	CCGG	3 cut(s)	84, 208, 244
BslI	CCNNNNNNNGG	3 cut(s)	46, 90, 226
BsnI	GGCC	3 cut(s)	53, 75, 177
Bsp143I	GATC	1 cut(s)	280
BspACI	CCGC	9 cut(s)	54, 60, 62, 87, 146, 230, 258, 300, 302
BspANI	GGCC	3 cut(s)	53, 75, 177
BspDI	ATCGAT	1 cut(s)	367
BspFNI	CGCG	2 cut(s)	62, 302
BspLI	GGNNCC	2 cut(s)	74, 96
BspPI	GGATC	1 cut(s)	275
BspT107I	GGYRCC	1 cut(s)	94
BsrFI	RCCGGY	2 cut(s)	83, 243
BssAI	RCCGGY	2 cut(s)	83, 243
BssECI	CCNNGG	5 cut(s)	60, 172, 208, 273, 300
BssMI	GATC	1 cut(s)	280
BssNI	GRCGYC	1 cut(s)	95
BssT1I	CCWWGG	1 cut(s)	172
Bst2UI	CCWGG	1 cut(s)	221
BstACI	GRCGYC	1 cut(s)	95
BstAPI	GCANNNNNTGC	1 cut(s)	143
BstC8I	GCNNGC	3 cut(s)	164, 168, 232
BstDEI	CTNAG	1 cut(s)	261
BstDSI	CCRYGG	3 cut(s)	60, 273, 300
BstF5I	GGATG	1 cut(s)	21
BstFNI	CGCG	2 cut(s)	62, 302
BstH2I	RGCGCY	1 cut(s)	98
BstHHI	GCGC	2 cut(s)	6, 97
BstKTI	GATC	1 cut(s)	283
BstMBI	GATC	1 cut(s)	280
BstMWI	GCNNNNNNNGC	5 cut(s)	12, 59, 143, 299, 346
BstNI	CCWGG	1 cut(s)	221
BstSCI	CCNGG	2 cut(s)	207, 219
BstUI	CGCG	2 cut(s)	62, 302
BstV1I	GCAGC	1 cut(s)	43
Bsu15I	ATCGAT	1 cut(s)	367
BsuRI	GGCC	3 cut(s)	53, 75, 177
BsuTUI	ATCGAT	1 cut(s)	367
BtgI	CCRYGG	3 cut(s)	60, 273, 300
BtsCI	GGATG	1 cut(s)	21
BtsI	GCAGTG	1 cut(s)	10
BtsIMutI	CAGTG	1 cut(s)	10
Cac8I	GCNNGC	3 cut(s)	164, 168, 232
CfoI	GCGC	2 cut(s)	6, 97
Cfr10I	RCCGGY	2 cut(s)	83, 243
Cfr13I	GGNCC	2 cut(s)	64, 73
Cfr42I	CCGCGG	2 cut(s)	63, 303
ClaI	ATCGAT	1 cut(s)	367
CpoI	CGGWCCG	1 cut(s)	64
Csp6I	GTAC	1 cut(s)	157
CspI	CGGWCCG	1 cut(s)	64
CviAII	CATG	1 cut(s)	122
CviJI	RGCY	9 cut(s)	53, 75, 170, 177, 219, 247, 278, 332, 379
CviKI_1	RGCY	9 cut(s)	53, 75, 170, 177, 219, 247, 278, 332, 379
CviQI	GTAC	1 cut(s)	157
DdeI	CTNAG	1 cut(s)	261
DinI	GGCGCC	1 cut(s)	96
DpnI	GATC	1 cut(s)	282
DpnII	GATC	1 cut(s)	280
EaeI	YGGCCR	1 cut(s)	51
Eco130I	CCWWGG	1 cut(s)	172
Eco32I	GATATC	1 cut(s)	357
Eco47I	GGWCC	1 cut(s)	64
EcoO109I	RGGNCCY	1 cut(s)	73
EcoRII	CCWGG	1 cut(s)	219
EcoRV	GATATC	1 cut(s)	357
EcoT14I	CCWWGG	1 cut(s)	172
EgeI	GGCGCC	1 cut(s)	96
EheI	GGCGCC	1 cut(s)	96
ErhI	CCWWGG	1 cut(s)	172
FaeI	CATG	1 cut(s)	125
FaiI	YATR	3 cut(s)	80, 101, 123
FatI	CATG	1 cut(s)	121
FauI	CCCGC	2 cut(s)	223, 307
Fnu4HI	GCNGC	3 cut(s)	54, 57, 60
FokI	GGATG	1 cut(s)	8
Fsp4HI	GCNGC	3 cut(s)	54, 57, 60
FspBI	CTAG	1 cut(s)	385
GlaI	GCGC	2 cut(s)	5, 96
GluI	GCNGC	3 cut(s)	54, 57, 60
HaeII	RGCGCY	1 cut(s)	98
HaeIII	GGCC	3 cut(s)	53, 75, 177
HapII	CCGG	3 cut(s)	84, 208, 244
HhaI	GCGC	2 cut(s)	6, 97
Hin1I	GRCGYC	1 cut(s)	95
Hin1II	CATG	1 cut(s)	125
Hin6I	GCGC	2 cut(s)	4, 95
HinP1I	GCGC	2 cut(s)	4, 95
HinfI	GANTC	1 cut(s)	201
HpaII	CCGG	3 cut(s)	84, 208, 244
Hpy188I	TCNGA	1 cut(s)	154
Hpy188III	TCNNGA	1 cut(s)	284
Hpy99I	CGWCG	1 cut(s)	72
HpyAV	CCTTC	2 cut(s)	50, 322
HpyCH4V	TGCA	1 cut(s)	137
HpyF10VI	GCNNNNNNNGC	5 cut(s)	12, 59, 143, 299, 346
HpyF3I	CTNAG	1 cut(s)	261
Hsp92I	GRCGYC	1 cut(s)	95
Hsp92II	CATG	1 cut(s)	125
HspAI	GCGC	2 cut(s)	4, 95
KasI	GGCGCC	1 cut(s)	94
KspI	CCGCGG	2 cut(s)	63, 303
Kzo9I	GATC	1 cut(s)	280
LmnI	GCTCC	1 cut(s)	337
LpnPI	CCDG	7 cut(s)	32, 97, 191, 206, 221, 233, 257
Lsp1109I	GCAGC	1 cut(s)	43
LweI	GCATC	1 cut(s)	243
MaeI	CTAG	1 cut(s)	385
MalI	GATC	1 cut(s)	282
MboI	GATC	1 cut(s)	280
Mly113I	GGCGCC	1 cut(s)	95
MlyI	GAGTC	1 cut(s)	210
MmeI	TCCRAC	1 cut(s)	132
MnlI	CCTC	8 cut(s)	18, 26, 29, 35, 41, 47, 54, 304
MseI	TTAA	1 cut(s)	345
MspA1I	CMGCKG	3 cut(s)	56, 62, 302
MspI	CCGG	3 cut(s)	84, 208, 244
MspR9I	CCNGG	2 cut(s)	209, 221
MvaI	CCWGG	1 cut(s)	221
MvnI	CGCG	2 cut(s)	62, 302
MwoI	GCNNNNNNNGC	5 cut(s)	12, 59, 143, 299, 346
NarI	GGCGCC	1 cut(s)	95
NciI	CCSGG	1 cut(s)	209
NdeII	GATC	1 cut(s)	280
NlaIII	CATG	1 cut(s)	125
NlaIV	GGNNCC	2 cut(s)	74, 96
PcsI	WCGNNNNNNNCGW	1 cut(s)	210
PflMI	CCANNNNNTGG	1 cut(s)	226
PkrI	GCNGC	3 cut(s)	55, 58, 61
PleI	GAGTC	1 cut(s)	209
PluTI	GGCGCC	1 cut(s)	98
PpsI	GAGTC	1 cut(s)	209
PsiI	TTATAA	1 cut(s)	80
Psp6I	CCWGG	1 cut(s)	219
PspGI	CCWGG	1 cut(s)	219
PspN4I	GGNNCC	2 cut(s)	74, 96
PspPI	GGNCC	2 cut(s)	64, 73
RsaI	GTAC	1 cut(s)	158
RsaNI	GTAC	1 cut(s)	157
Rsr2I	CGGWCCG	1 cut(s)	64
RsrII	CGGWCCG	1 cut(s)	64
SacII	CCGCGG	2 cut(s)	63, 303
SaqAI	TTAA	1 cut(s)	345
SatI	GCNGC	3 cut(s)	54, 57, 60
Sau3AI	GATC	1 cut(s)	280
Sau96I	GGNCC	2 cut(s)	64, 73
SchI	GAGTC	1 cut(s)	210
ScrFI	CCNGG	2 cut(s)	209, 221
SetI	ASST	1 cut(s)	225
SfaNI	GCATC	1 cut(s)	243
SfoI	GGCGCC	1 cut(s)	96
Sfr303I	CCGCGG	2 cut(s)	63, 303
SgrBI	CCGCGG	2 cut(s)	63, 303
SinI	GGWCC	1 cut(s)	64
SsiI	CCGC	9 cut(s)	54, 60, 62, 87, 146, 230, 258, 300, 302
SspDI	GGCGCC	1 cut(s)	94
SspMI	CTAG	1 cut(s)	385
StyD4I	CCNGG	2 cut(s)	207, 219
StyI	CCWWGG	1 cut(s)	172
TaqI	TCGA	1 cut(s)	367
TaqII	GACCGA	1 cut(s)	81
TatI	WGTACW	1 cut(s)	156
TauI	GCSGC	2 cut(s)	56, 62
Tru1I	TTAA	1 cut(s)	345
Tru9I	TTAA	1 cut(s)	345
TscAI	CASTG	1 cut(s)	17
TseI	GCWGC	1 cut(s)	56
TspDTI	ATGAA	1 cut(s)	353
TspGWI	ACGGA	1 cut(s)	262
TspRI	CASTG	1 cut(s)	17
Van91I	CCANNNNNTGG	1 cut(s)	226
VpaK11BI	GGWCC	1 cut(s)	64
XspI	CTAG	1 cut(s)	385

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.