Belongs to the cystatin family. Phytocystatin subfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 392 |
| AccI | GTMKAC | 1 cut(s) | 114 |
| AccII | CGCG | 2 cut(s) | 26, 131 |
| AciI | CCGC | 1 cut(s) | 131 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 109, 288 |
| AgsI | TTSAA | 2 cut(s) | 176, 344 |
| AjnI | CCWGG | 3 cut(s) | 301, 396, 408 |
| AluBI | AGCT | 1 cut(s) | 368 |
| AluI | AGCT | 1 cut(s) | 368 |
| Alw21I | GWGCWC | 1 cut(s) | 21 |
| Alw26I | GTCTC | 1 cut(s) | 139 |
| AoxI | GGCC | 2 cut(s) | 237, 258 |
| ApeKI | GCWGC | 1 cut(s) | 332 |
| AspLEI | GCGC | 1 cut(s) | 9 |
| AspS9I | GGNCC | 3 cut(s) | 292, 394, 412 |
| AsuHPI | GGTGA | 2 cut(s) | 246, 338 |
| AvaII | GGWCC | 3 cut(s) | 292, 394, 412 |
| Bbv12I | GWGCWC | 1 cut(s) | 21 |
| BbvI | GCAGC | 1 cut(s) | 319 |
| BccI | CCATC | 3 cut(s) | 306, 375, 430 |
| BciT130I | CCWGG | 3 cut(s) | 303, 398, 410 |
| BcoDI | GTCTC | 1 cut(s) | 139 |
| BfaI | CTAG | 1 cut(s) | 416 |
| BisI | GCNGC | 1 cut(s) | 333 |
| BlsI | GCNGC | 1 cut(s) | 334 |
| Bme1390I | CCNGG | 3 cut(s) | 303, 398, 410 |
| Bme18I | GGWCC | 3 cut(s) | 292, 394, 412 |
| BmgT120I | GGNCC | 3 cut(s) | 292, 394, 412 |
| BmiI | GGNNCC | 2 cut(s) | 293, 441 |
| BmrFI | CCNGG | 3 cut(s) | 303, 398, 410 |
| BmsI | GCATC | 1 cut(s) | 222 |
| BsaI | GGTCTC | 1 cut(s) | 139 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 109, 288 |
| Bse118I | RCCGGY | 1 cut(s) | 256 |
| BseBI | CCWGG | 3 cut(s) | 303, 398, 410 |
| BseGI | GGATG | 1 cut(s) | 298 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 109, 288 |
| BseRI | GAGGAG | 1 cut(s) | 11 |
| BseXI | GCAGC | 1 cut(s) | 319 |
| Bsh1236I | CGCG | 2 cut(s) | 26, 131 |
| Bsh1285I | CGRYCG | 1 cut(s) | 64 |
| BshFI | GGCC | 2 cut(s) | 239, 260 |
| BsiEI | CGRYCG | 1 cut(s) | 64 |
| BsiHKAI | GWGCWC | 1 cut(s) | 21 |
| BsiSI | CCGG | 1 cut(s) | 257 |
| BslFI | GGGAC | 2 cut(s) | 305, 398 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 109, 288 |
| BsmAI | GTCTC | 1 cut(s) | 139 |
| BsmFI | GGGAC | 2 cut(s) | 305, 398 |
| BsnI | GGCC | 2 cut(s) | 239, 260 |
| Bso31I | GGTCTC | 1 cut(s) | 139 |
| Bsp1286I | GDGCHC | 1 cut(s) | 21 |
| Bsp143I | GATC | 2 cut(s) | 61, 346 |
| BspACI | CCGC | 1 cut(s) | 131 |
| BspANI | GGCC | 2 cut(s) | 239, 260 |
| BspFNI | CGCG | 2 cut(s) | 26, 131 |
| BspLI | GGNNCC | 2 cut(s) | 293, 441 |
| BspQI | GCTCTTC | 1 cut(s) | 42 |
| BspTNI | GGTCTC | 1 cut(s) | 139 |
| BsrFI | RCCGGY | 1 cut(s) | 256 |
| BssAI | RCCGGY | 1 cut(s) | 256 |
| BssMI | GATC | 2 cut(s) | 61, 346 |
| Bst2UI | CCWGG | 3 cut(s) | 303, 398, 410 |
| Bst4CI | ACNGT | 1 cut(s) | 283 |
| Bst6I | CTCTTC | 2 cut(s) | 42, 256 |
| BstC8I | GCNNGC | 2 cut(s) | 33, 211 |
| BstF5I | GGATG | 1 cut(s) | 298 |
| BstFNI | CGCG | 2 cut(s) | 26, 131 |
| BstHHI | GCGC | 1 cut(s) | 9 |
| BstKTI | GATC | 2 cut(s) | 64, 349 |
| BstMAI | GTCTC | 1 cut(s) | 139 |
| BstMBI | GATC | 2 cut(s) | 61, 346 |
| BstMCI | CGRYCG | 1 cut(s) | 64 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 32, 128, 236, 266 |
| BstNI | CCWGG | 3 cut(s) | 303, 398, 410 |
| BstSCI | CCNGG | 3 cut(s) | 301, 396, 408 |
| BstUI | CGCG | 2 cut(s) | 26, 131 |
| BstV1I | GCAGC | 1 cut(s) | 319 |
| BsuRI | GGCC | 2 cut(s) | 239, 260 |
| BtsCI | GGATG | 1 cut(s) | 298 |
| BtsI | GCAGTG | 1 cut(s) | 117 |
| BtsIMutI | CAGTG | 1 cut(s) | 117 |
| Cac8I | GCNNGC | 2 cut(s) | 33, 211 |
| CfoI | GCGC | 1 cut(s) | 9 |
| Cfr10I | RCCGGY | 1 cut(s) | 256 |
| Cfr13I | GGNCC | 3 cut(s) | 292, 394, 412 |
| CviAII | CATG | 2 cut(s) | 14, 388 |
| CviJI | RGCY | 8 cut(s) | 82, 155, 239, 260, 306, 332, 368, 440 |
| CviKI_1 | RGCY | 8 cut(s) | 82, 155, 239, 260, 306, 332, 368, 440 |
| DpnI | GATC | 2 cut(s) | 63, 348 |
| DpnII | GATC | 2 cut(s) | 61, 346 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 392 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 392 |
| Eam1104I | CTCTTC | 2 cut(s) | 42, 256 |
| EarI | CTCTTC | 2 cut(s) | 42, 256 |
| Eco31I | GGTCTC | 1 cut(s) | 139 |
| Eco32I | GATATC | 1 cut(s) | 197 |
| Eco47I | GGWCC | 3 cut(s) | 292, 394, 412 |
| EcoRII | CCWGG | 3 cut(s) | 301, 396, 408 |
| EcoRV | GATATC | 1 cut(s) | 197 |
| FaeI | CATG | 2 cut(s) | 17, 391 |
| FaiI | YATR | 6 cut(s) | 15, 86, 222, 276, 389, 454 |
| FaqI | GGGAC | 2 cut(s) | 305, 398 |
| FatI | CATG | 2 cut(s) | 13, 387 |
| FblI | GTMKAC | 1 cut(s) | 114 |
| Fnu4HI | GCNGC | 1 cut(s) | 333 |
| FokI | GGATG | 1 cut(s) | 285 |
| Fsp4HI | GCNGC | 1 cut(s) | 333 |
| FspBI | CTAG | 1 cut(s) | 416 |
| GlaI | GCGC | 1 cut(s) | 8 |
| GluI | GCNGC | 1 cut(s) | 333 |
| HaeIII | GGCC | 2 cut(s) | 239, 260 |
| HapII | CCGG | 1 cut(s) | 257 |
| HhaI | GCGC | 1 cut(s) | 9 |
| Hin1II | CATG | 2 cut(s) | 17, 391 |
| Hin6I | GCGC | 1 cut(s) | 7 |
| HinP1I | GCGC | 1 cut(s) | 7 |
| HinfI | GANTC | 1 cut(s) | 248 |
| HpaII | CCGG | 1 cut(s) | 257 |
| HphI | GGTGA | 2 cut(s) | 246, 338 |
| Hpy166II | GTNNAC | 1 cut(s) | 115 |
| Hpy188I | TCNGA | 1 cut(s) | 139 |
| Hpy8I | GTNNAC | 1 cut(s) | 115 |
| HpyAV | CCTTC | 1 cut(s) | 345 |
| HpyCH4III | ACNGT | 1 cut(s) | 283 |
| HpyCH4V | TGCA | 3 cut(s) | 122, 209, 269 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 32, 128, 236, 266 |
| Hsp92II | CATG | 2 cut(s) | 17, 391 |
| HspAI | GCGC | 1 cut(s) | 7 |
| Kzo9I | GATC | 2 cut(s) | 61, 346 |
| LguI | GCTCTTC | 1 cut(s) | 42 |
| LmnI | GCTCC | 2 cut(s) | 24, 445 |
| Lsp1109I | GCAGC | 1 cut(s) | 319 |
| LweI | GCATC | 1 cut(s) | 222 |
| MaeI | CTAG | 1 cut(s) | 416 |
| MaeIII | GTNAC | 1 cut(s) | 277 |
| MalI | GATC | 2 cut(s) | 63, 348 |
| MboI | GATC | 2 cut(s) | 61, 346 |
| MboII | GAAGA | 3 cut(s) | 29, 243, 356 |
| MhlI | GDGCHC | 1 cut(s) | 21 |
| MluCI | AATT | 1 cut(s) | 271 |
| MlyI | GAGTC | 1 cut(s) | 242 |
| MnlI | CCTC | 8 cut(s) | 21, 32, 57, 142, 176, 181, 271, 322 |
| MseI | TTAA | 1 cut(s) | 204 |
| MspI | CCGG | 1 cut(s) | 257 |
| MspR9I | CCNGG | 3 cut(s) | 303, 398, 410 |
| MvaI | CCWGG | 3 cut(s) | 303, 398, 410 |
| MvnI | CGCG | 2 cut(s) | 26, 131 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 32, 128, 236, 266 |
| NdeII | GATC | 2 cut(s) | 61, 346 |
| NlaIII | CATG | 2 cut(s) | 17, 391 |
| NlaIV | GGNNCC | 2 cut(s) | 293, 441 |
| PciSI | GCTCTTC | 1 cut(s) | 42 |
| PkrI | GCNGC | 1 cut(s) | 334 |
| Ple19I | CGATCG | 1 cut(s) | 64 |
| PleI | GAGTC | 1 cut(s) | 242 |
| PpsI | GAGTC | 1 cut(s) | 242 |
| Psp6I | CCWGG | 3 cut(s) | 301, 396, 408 |
| PspGI | CCWGG | 3 cut(s) | 301, 396, 408 |
| PspN4I | GGNNCC | 2 cut(s) | 293, 441 |
| PspPI | GGNCC | 3 cut(s) | 292, 394, 412 |
| PvuI | CGATCG | 1 cut(s) | 64 |
| SapI | GCTCTTC | 1 cut(s) | 42 |
| SaqAI | TTAA | 1 cut(s) | 204 |
| SatI | GCNGC | 1 cut(s) | 333 |
| Sau3AI | GATC | 2 cut(s) | 61, 346 |
| Sau96I | GGNCC | 3 cut(s) | 292, 394, 412 |
| SchI | GAGTC | 1 cut(s) | 242 |
| ScrFI | CCNGG | 3 cut(s) | 303, 398, 410 |
| SduI | GDGCHC | 1 cut(s) | 21 |
| SetI | ASST | 4 cut(s) | 168, 356, 370, 417 |
| SfaNI | GCATC | 1 cut(s) | 222 |
| SinI | GGWCC | 3 cut(s) | 292, 394, 412 |
| Sse9I | AATT | 1 cut(s) | 271 |
| SsiI | CCGC | 1 cut(s) | 131 |
| SspMI | CTAG | 1 cut(s) | 416 |
| StyD4I | CCNGG | 3 cut(s) | 301, 396, 408 |
| TaaI | ACNGT | 1 cut(s) | 283 |
| TaqI | TCGA | 2 cut(s) | 186, 349 |
| TasI | AATT | 1 cut(s) | 271 |
| Tru1I | TTAA | 1 cut(s) | 204 |
| Tru9I | TTAA | 1 cut(s) | 204 |
| TscAI | CASTG | 1 cut(s) | 124 |
| TseI | GCWGC | 1 cut(s) | 332 |
| TspDTI | ATGAA | 1 cut(s) | 237 |
| TspRI | CASTG | 1 cut(s) | 124 |
| VpaK11BI | GGWCC | 3 cut(s) | 292, 394, 412 |
| XmiI | GTMKAC | 1 cut(s) | 114 |
| XspI | CTAG | 1 cut(s) | 416 |