Belongs to the cystatin family. Phytocystatin subfamily
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 74 |
| AccB1I | GGYRCC | 1 cut(s) | 9 |
| AccII | CGCG | 1 cut(s) | 149 |
| AciI | CCGC | 2 cut(s) | 13, 149 |
| AclWI | GGATC | 1 cut(s) | 113 |
| AfaI | GTAC | 1 cut(s) | 161 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 49, 130 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 167, 199 |
| Alw26I | GTCTC | 2 cut(s) | 91, 248 |
| AlwI | GGATC | 1 cut(s) | 113 |
| AoxI | GGCC | 1 cut(s) | 57 |
| AspS9I | GGNCC | 1 cut(s) | 202 |
| AsuC2I | CCSGG | 1 cut(s) | 206 |
| AsuHPI | GGTGA | 1 cut(s) | 128 |
| AvaII | GGWCC | 1 cut(s) | 202 |
| BanI | GGYRCC | 1 cut(s) | 9 |
| BccI | CCATC | 1 cut(s) | 303 |
| BcnI | CCSGG | 1 cut(s) | 206 |
| BcoDI | GTCTC | 2 cut(s) | 91, 248 |
| Bme1390I | CCNGG | 1 cut(s) | 206 |
| Bme18I | GGWCC | 1 cut(s) | 202 |
| BmgT120I | GGNCC | 1 cut(s) | 202 |
| BmiI | GGNNCC | 1 cut(s) | 11 |
| BmrFI | CCNGG | 1 cut(s) | 206 |
| BmsI | GCATC | 1 cut(s) | 240 |
| BpuMI | CCSGG | 1 cut(s) | 206 |
| BsaI | GGTCTC | 1 cut(s) | 91 |
| BsaJI | CCNNGG | 1 cut(s) | 205 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 49, 130 |
| BseDI | CCNNGG | 1 cut(s) | 205 |
| BseGI | GGATG | 1 cut(s) | 318 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 49, 130 |
| BseRI | GAGGAG | 1 cut(s) | 29 |
| Bsh1236I | CGCG | 1 cut(s) | 149 |
| BshFI | GGCC | 1 cut(s) | 59 |
| BshNI | GGYRCC | 1 cut(s) | 9 |
| BsiSI | CCGG | 1 cut(s) | 205 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 49, 130 |
| BsmAI | GTCTC | 2 cut(s) | 91, 248 |
| BsmBI | CGTCTC | 1 cut(s) | 248 |
| BsnI | GGCC | 1 cut(s) | 59 |
| Bso31I | GGTCTC | 1 cut(s) | 91 |
| Bsp143I | GATC | 2 cut(s) | 118, 330 |
| BspACI | CCGC | 2 cut(s) | 13, 149 |
| BspANI | GGCC | 1 cut(s) | 59 |
| BspFNI | CGCG | 1 cut(s) | 149 |
| BspLI | GGNNCC | 1 cut(s) | 11 |
| BspPI | GGATC | 1 cut(s) | 113 |
| BspT107I | GGYRCC | 1 cut(s) | 9 |
| BspTNI | GGTCTC | 1 cut(s) | 91 |
| BssECI | CCNNGG | 1 cut(s) | 205 |
| BssMI | GATC | 2 cut(s) | 118, 330 |
| Bst6I | CTCTTC | 1 cut(s) | 299 |
| BstC8I | GCNNGC | 1 cut(s) | 229 |
| BstF5I | GGATG | 1 cut(s) | 318 |
| BstFNI | CGCG | 1 cut(s) | 149 |
| BstKTI | GATC | 2 cut(s) | 121, 333 |
| BstMAI | GTCTC | 2 cut(s) | 91, 248 |
| BstMBI | GATC | 2 cut(s) | 118, 330 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 32, 146 |
| BstSCI | CCNGG | 1 cut(s) | 204 |
| BstUI | CGCG | 1 cut(s) | 149 |
| BsuRI | GGCC | 1 cut(s) | 59 |
| BtsCI | GGATG | 1 cut(s) | 318 |
| Cac8I | GCNNGC | 1 cut(s) | 229 |
| Cfr13I | GGNCC | 1 cut(s) | 202 |
| Csp6I | GTAC | 1 cut(s) | 160 |
| CviAII | CATG | 1 cut(s) | 125 |
| CviJI | RGCY | 3 cut(s) | 35, 59, 290 |
| CviKI_1 | RGCY | 3 cut(s) | 35, 59, 290 |
| CviQI | GTAC | 1 cut(s) | 160 |
| DpnI | GATC | 2 cut(s) | 120, 332 |
| DpnII | GATC | 2 cut(s) | 118, 330 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 74 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 74 |
| Eam1104I | CTCTTC | 1 cut(s) | 299 |
| EarI | CTCTTC | 1 cut(s) | 299 |
| Eco147I | AGGCCT | 1 cut(s) | 59 |
| Eco31I | GGTCTC | 1 cut(s) | 91 |
| Eco47I | GGWCC | 1 cut(s) | 202 |
| Esp3I | CGTCTC | 1 cut(s) | 248 |
| FaeI | CATG | 1 cut(s) | 128 |
| FaiI | YATR | 3 cut(s) | 126, 285, 298 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 167, 199 |
| FatI | CATG | 1 cut(s) | 124 |
| FokI | GGATG | 1 cut(s) | 325 |
| HaeIII | GGCC | 1 cut(s) | 59 |
| HapII | CCGG | 1 cut(s) | 205 |
| Hin1II | CATG | 1 cut(s) | 128 |
| HincII | GTYRAC | 1 cut(s) | 259 |
| HindII | GTYRAC | 1 cut(s) | 259 |
| HinfI | GANTC | 4 cut(s) | 4, 84, 214, 269 |
| HpaII | CCGG | 1 cut(s) | 205 |
| HphI | GGTGA | 1 cut(s) | 128 |
| Hpy166II | GTNNAC | 1 cut(s) | 259 |
| Hpy188I | TCNGA | 3 cut(s) | 157, 195, 277 |
| Hpy8I | GTNNAC | 1 cut(s) | 259 |
| HpyAV | CCTTC | 3 cut(s) | 53, 70, 124 |
| HpyCH4V | TGCA | 1 cut(s) | 140 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 32, 146 |
| Hsp92II | CATG | 1 cut(s) | 128 |
| Kzo9I | GATC | 2 cut(s) | 118, 330 |
| LpnPI | CCDG | 5 cut(s) | 35, 185, 203, 213, 218 |
| LweI | GCATC | 1 cut(s) | 240 |
| MalI | GATC | 2 cut(s) | 120, 332 |
| MboI | GATC | 2 cut(s) | 118, 330 |
| MboII | GAAGA | 4 cut(s) | 187, 190, 275, 316 |
| MluCI | AATT | 3 cut(s) | 280, 326, 352 |
| MlyI | GAGTC | 3 cut(s) | 13, 93, 223 |
| MmeI | TCCRAC | 2 cut(s) | 74, 135 |
| MnlI | CCTC | 6 cut(s) | 32, 50, 57, 67, 280, 283 |
| MseI | TTAA | 1 cut(s) | 355 |
| MspI | CCGG | 1 cut(s) | 205 |
| MspR9I | CCNGG | 1 cut(s) | 206 |
| MvnI | CGCG | 1 cut(s) | 149 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 32, 146 |
| NciI | CCSGG | 1 cut(s) | 206 |
| NdeII | GATC | 2 cut(s) | 118, 330 |
| NlaIII | CATG | 1 cut(s) | 128 |
| NlaIV | GGNNCC | 1 cut(s) | 11 |
| PceI | AGGCCT | 1 cut(s) | 59 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 69 |
| PfeI | GAWTC | 1 cut(s) | 269 |
| PleI | GAGTC | 3 cut(s) | 12, 92, 222 |
| PpsI | GAGTC | 3 cut(s) | 12, 92, 222 |
| PspN4I | GGNNCC | 1 cut(s) | 11 |
| PspPI | GGNCC | 1 cut(s) | 202 |
| RsaI | GTAC | 1 cut(s) | 161 |
| RsaNI | GTAC | 1 cut(s) | 160 |
| SaqAI | TTAA | 1 cut(s) | 355 |
| Sau3AI | GATC | 2 cut(s) | 118, 330 |
| Sau96I | GGNCC | 1 cut(s) | 202 |
| SchI | GAGTC | 3 cut(s) | 13, 93, 223 |
| ScrFI | CCNGG | 1 cut(s) | 206 |
| SetI | ASST | 2 cut(s) | 78, 222 |
| SfaNI | GCATC | 1 cut(s) | 240 |
| SinI | GGWCC | 1 cut(s) | 202 |
| Sse9I | AATT | 3 cut(s) | 280, 326, 352 |
| SseBI | AGGCCT | 1 cut(s) | 59 |
| SsiI | CCGC | 2 cut(s) | 13, 149 |
| StuI | AGGCCT | 1 cut(s) | 59 |
| StyD4I | CCNGG | 1 cut(s) | 204 |
| TasI | AATT | 3 cut(s) | 280, 326, 352 |
| TatI | WGTACW | 1 cut(s) | 159 |
| TfiI | GAWTC | 1 cut(s) | 269 |
| Tru1I | TTAA | 1 cut(s) | 355 |
| Tru9I | TTAA | 1 cut(s) | 355 |
| TspDTI | ATGAA | 2 cut(s) | 329, 357 |
| VpaK11BI | GGWCC | 1 cut(s) | 202 |