Belongs to the cystatin family. Phytocystatin subfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 246 |
| AarI | CACCTGC | 1 cut(s) | 83 |
| Acc36I | ACCTGC | 1 cut(s) | 83 |
| AciI | CCGC | 3 cut(s) | 128, 155, 481 |
| AclWI | GGATC | 3 cut(s) | 65, 267, 280 |
| AcuI | CTGAAG | 1 cut(s) | 99 |
| AfaI | GTAC | 2 cut(s) | 10, 167 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 52, 205 |
| AgsI | TTSAA | 2 cut(s) | 212, 337 |
| AjnI | CCWGG | 1 cut(s) | 216 |
| AluBI | AGCT | 5 cut(s) | 62, 233, 250, 360, 430 |
| AluI | AGCT | 5 cut(s) | 62, 233, 250, 360, 430 |
| Alw26I | GTCTC | 1 cut(s) | 314 |
| AlwI | GGATC | 3 cut(s) | 65, 267, 280 |
| AoxI | GGCC | 1 cut(s) | 214 |
| ApeKI | GCWGC | 3 cut(s) | 62, 357, 365 |
| AsuHPI | GGTGA | 2 cut(s) | 134, 151 |
| AxyI | CCTNAGG | 1 cut(s) | 200 |
| BamHI | GGATCC | 1 cut(s) | 272 |
| BbsI | GAAGAC | 2 cut(s) | 87, 423 |
| BbvI | GCAGC | 3 cut(s) | 49, 369, 377 |
| BccI | CCATC | 3 cut(s) | 82, 116, 263 |
| BciT130I | CCWGG | 1 cut(s) | 218 |
| BcoDI | GTCTC | 1 cut(s) | 314 |
| BfaI | CTAG | 2 cut(s) | 327, 450 |
| BfuAI | ACCTGC | 1 cut(s) | 83 |
| BisI | GCNGC | 3 cut(s) | 63, 358, 366 |
| BlsI | GCNGC | 3 cut(s) | 64, 359, 367 |
| Bme1390I | CCNGG | 1 cut(s) | 218 |
| BmiI | GGNNCC | 2 cut(s) | 105, 274 |
| BmrFI | CCNGG | 1 cut(s) | 218 |
| BpiI | GAAGAC | 2 cut(s) | 87, 423 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 52, 205 |
| Bse21I | CCTNAGG | 1 cut(s) | 200 |
| BseBI | CCWGG | 1 cut(s) | 218 |
| BseGI | GGATG | 1 cut(s) | 274 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 52, 205 |
| BseMII | CTCAG | 2 cut(s) | 26, 191 |
| BseRI | GAGGAG | 1 cut(s) | 32 |
| BseXI | GCAGC | 3 cut(s) | 49, 369, 377 |
| BseYI | CCCAGC | 1 cut(s) | 360 |
| BshFI | GGCC | 1 cut(s) | 216 |
| BsiSI | CCGG | 1 cut(s) | 66 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 52, 205 |
| BsmAI | GTCTC | 1 cut(s) | 314 |
| BsnI | GGCC | 1 cut(s) | 216 |
| Bsp143I | GATC | 2 cut(s) | 70, 272 |
| BspACI | CCGC | 3 cut(s) | 128, 155, 481 |
| BspANI | GGCC | 1 cut(s) | 216 |
| BspCNI | CTCAG | 2 cut(s) | 25, 192 |
| BspLI | GGNNCC | 2 cut(s) | 105, 274 |
| BspMI | ACCTGC | 1 cut(s) | 83 |
| BspPI | GGATC | 3 cut(s) | 65, 267, 280 |
| BssMI | GATC | 2 cut(s) | 70, 272 |
| Bst2UI | CCWGG | 1 cut(s) | 218 |
| Bst4CI | ACNGT | 1 cut(s) | 283 |
| Bst6I | CTCTTC | 1 cut(s) | 26 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 35 |
| BstDEI | CTNAG | 2 cut(s) | 12, 200 |
| BstEII | GGTNACC | 2 cut(s) | 122, 157 |
| BstF5I | GGATG | 1 cut(s) | 274 |
| BstKTI | GATC | 2 cut(s) | 73, 275 |
| BstMAI | GTCTC | 1 cut(s) | 314 |
| BstMBI | GATC | 2 cut(s) | 70, 272 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 35, 152 |
| BstNI | CCWGG | 1 cut(s) | 218 |
| BstNSI | RCATGY | 1 cut(s) | 134 |
| BstPI | GGTNACC | 2 cut(s) | 122, 157 |
| BstSCI | CCNGG | 1 cut(s) | 216 |
| BstV1I | GCAGC | 3 cut(s) | 49, 369, 377 |
| BstV2I | GAAGAC | 2 cut(s) | 87, 423 |
| BstX2I | RGATCY | 2 cut(s) | 70, 272 |
| BstYI | RGATCY | 2 cut(s) | 70, 272 |
| Bsu36I | CCTNAGG | 1 cut(s) | 200 |
| BsuRI | GGCC | 1 cut(s) | 216 |
| BtsCI | GGATG | 1 cut(s) | 274 |
| BtsIMutI | CAGTG | 1 cut(s) | 20 |
| BveI | ACCTGC | 1 cut(s) | 83 |
| Csp6I | GTAC | 2 cut(s) | 9, 166 |
| CviAII | CATG | 1 cut(s) | 131 |
| CviJI | RGCY | 7 cut(s) | 62, 216, 233, 250, 297, 360, 430 |
| CviKI_1 | RGCY | 7 cut(s) | 62, 216, 233, 250, 297, 360, 430 |
| CviQI | GTAC | 2 cut(s) | 9, 166 |
| DdeI | CTNAG | 2 cut(s) | 12, 200 |
| DpnI | GATC | 2 cut(s) | 72, 274 |
| DpnII | GATC | 2 cut(s) | 70, 272 |
| Eam1104I | CTCTTC | 1 cut(s) | 26 |
| EarI | CTCTTC | 1 cut(s) | 26 |
| Eco57I | CTGAAG | 1 cut(s) | 99 |
| Eco81I | CCTNAGG | 1 cut(s) | 200 |
| Eco91I | GGTNACC | 2 cut(s) | 122, 157 |
| EcoO65I | GGTNACC | 2 cut(s) | 122, 157 |
| EcoRII | CCWGG | 1 cut(s) | 216 |
| FaeI | CATG | 1 cut(s) | 134 |
| FaiI | YATR | 8 cut(s) | 59, 132, 143, 246, 291, 351, 399, 490 |
| FatI | CATG | 1 cut(s) | 130 |
| FauI | CCCGC | 1 cut(s) | 135 |
| Fnu4HI | GCNGC | 3 cut(s) | 63, 358, 366 |
| FokI | GGATG | 1 cut(s) | 281 |
| Fsp4HI | GCNGC | 3 cut(s) | 63, 358, 366 |
| FspBI | CTAG | 2 cut(s) | 327, 450 |
| GluI | GCNGC | 3 cut(s) | 63, 358, 366 |
| GsaI | CCCAGC | 1 cut(s) | 364 |
| HaeIII | GGCC | 1 cut(s) | 216 |
| HapII | CCGG | 1 cut(s) | 66 |
| Hin1II | CATG | 1 cut(s) | 134 |
| HindIII | AAGCTT | 1 cut(s) | 248 |
| HinfI | GANTC | 2 cut(s) | 433, 453 |
| HpaII | CCGG | 1 cut(s) | 66 |
| HphI | GGTGA | 2 cut(s) | 134, 151 |
| Hpy188I | TCNGA | 1 cut(s) | 79 |
| Hpy188III | TCNNGA | 4 cut(s) | 112, 199, 327, 450 |
| HpyAV | CCTTC | 1 cut(s) | 56 |
| HpyCH4III | ACNGT | 1 cut(s) | 283 |
| HpyCH4V | TGCA | 3 cut(s) | 29, 92, 136 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 35, 152 |
| HpyF3I | CTNAG | 2 cut(s) | 12, 200 |
| Hsp92II | CATG | 1 cut(s) | 134 |
| Kzo9I | GATC | 2 cut(s) | 70, 272 |
| LpnPI | CCDG | 9 cut(s) | 38, 78, 79, 185, 203, 212, 219, 230, 346 |
| Lsp1109I | GCAGC | 3 cut(s) | 49, 369, 377 |
| MaeI | CTAG | 2 cut(s) | 327, 450 |
| MaeIII | GTNAC | 2 cut(s) | 122, 157 |
| MalI | GATC | 2 cut(s) | 72, 274 |
| MboI | GATC | 2 cut(s) | 70, 272 |
| MboII | GAAGA | 4 cut(s) | 13, 92, 428, 438 |
| MfeI | CAATTG | 1 cut(s) | 464 |
| MflI | RGATCY | 2 cut(s) | 70, 272 |
| MluCI | AATT | 5 cut(s) | 87, 241, 286, 332, 464 |
| MnlI | CCTC | 7 cut(s) | 29, 53, 60, 195, 286, 286, 431 |
| MspA1I | CMGCKG | 1 cut(s) | 360 |
| MspI | CCGG | 1 cut(s) | 66 |
| MspR9I | CCNGG | 1 cut(s) | 218 |
| MunI | CAATTG | 1 cut(s) | 464 |
| MvaI | CCWGG | 1 cut(s) | 218 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 35, 152 |
| NdeII | GATC | 2 cut(s) | 70, 272 |
| NlaIII | CATG | 1 cut(s) | 134 |
| NlaIV | GGNNCC | 2 cut(s) | 105, 274 |
| NmuCI | GTSAC | 2 cut(s) | 122, 157 |
| NspI | RCATGY | 1 cut(s) | 134 |
| PaqCI | CACCTGC | 1 cut(s) | 83 |
| PfeI | GAWTC | 2 cut(s) | 433, 453 |
| PkrI | GCNGC | 3 cut(s) | 64, 359, 367 |
| PsiI | TTATAA | 1 cut(s) | 246 |
| Psp6I | CCWGG | 1 cut(s) | 216 |
| PspEI | GGTNACC | 2 cut(s) | 122, 157 |
| PspFI | CCCAGC | 1 cut(s) | 360 |
| PspGI | CCWGG | 1 cut(s) | 216 |
| PspN4I | GGNNCC | 2 cut(s) | 105, 274 |
| PsuI | RGATCY | 2 cut(s) | 70, 272 |
| PvuII | CAGCTG | 1 cut(s) | 360 |
| RsaI | GTAC | 2 cut(s) | 10, 167 |
| RsaNI | GTAC | 2 cut(s) | 9, 166 |
| SatI | GCNGC | 3 cut(s) | 63, 358, 366 |
| Sau3AI | GATC | 2 cut(s) | 70, 272 |
| ScrFI | CCNGG | 1 cut(s) | 218 |
| SetI | ASST | 7 cut(s) | 64, 97, 235, 252, 362, 423, 432 |
| Sse9I | AATT | 5 cut(s) | 87, 241, 286, 332, 464 |
| SsiI | CCGC | 3 cut(s) | 128, 155, 481 |
| SspMI | CTAG | 2 cut(s) | 327, 450 |
| StyD4I | CCNGG | 1 cut(s) | 216 |
| TaaI | ACNGT | 1 cut(s) | 283 |
| TaqI | TCGA | 1 cut(s) | 423 |
| TasI | AATT | 5 cut(s) | 87, 241, 286, 332, 464 |
| TatI | WGTACW | 1 cut(s) | 165 |
| TfiI | GAWTC | 2 cut(s) | 433, 453 |
| TscAI | CASTG | 1 cut(s) | 20 |
| TseFI | GTSAC | 2 cut(s) | 122, 157 |
| TseI | GCWGC | 3 cut(s) | 62, 357, 365 |
| Tsp45I | GTSAC | 2 cut(s) | 122, 157 |
| TspDTI | ATGAA | 2 cut(s) | 369, 429 |
| TspRI | CASTG | 1 cut(s) | 20 |
| XbaI | TCTAGA | 2 cut(s) | 326, 449 |
| XceI | RCATGY | 1 cut(s) | 134 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 92 |
| XspI | CTAG | 2 cut(s) | 327, 450 |