Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0000425

Physical Location & Seq

Genomic Coordinates Forward (+)

16459138 .. 16459515

Gene Structure 378 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1148300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 378 bp

ATGCGCCCTTACTGCCTCCTCATCCTCGCCTTCGTCCTCCTTCCTCTGGTGGCTGCTGATGTGCACCCACGGGGCGGTTGGCAGCCCATAAAGAACATCAGCGACCTTCATGTGAAAGAGATCGCAGAGTTTGCGGTGTTGGAGTACAACAAGCATGCCCAAGGACAGAACAAGTTGGCGTTTGAGAGCGTAGTCCAGGGTGACAGCCAGGTAGTGGCAGGCATCAACTACCGGCTAGTCATTTCTGCCAAGAATGAGTCGGTAGTTGATCCCGCTGTTGCCACGCCCACCAGTGATTATGAGGGCATTGTGTGGGAGAAGGCTTGGGAGCATTTTAGGCAGTTGATTTCATTTCATCAATTGTTAAAACCTAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002020 GO:0003674 GO:0004857 GO:0004866 GO:0004869 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005623 GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0009892 GO:0009987 GO:0010029 GO:0010030 GO:0010466 GO:0010605 GO:0010951 GO:0019222 GO:0019899 GO:0030162 GO:0030234 GO:0030312 GO:0030414 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0033554 GO:0034605 GO:0043086 GO:0044092 GO:0044464 GO:0045861 GO:0048518 GO:0048519 GO:0048523 GO:0048580 GO:0048582 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0051716 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0065007 GO:0065009 GO:0071944 GO:0080090 GO:0098772 GO:1900140 GO:2000026 GO:2000116 GO:2000117

KEGG Pathways

Metabolic & Signaling

ko04970 map04970

Pfam Domains

Protein Families

SQAPI

Protein Analysis

126

Amino Acids

14.05

Weight (kDa)

6.18

Isoelectric Point (pI)

24.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cystatin	PF00031	25 - 113	5.6e-12	Cystatin domain
SQAPI	PF16845	30 - 120	4.2e-29	Aspartic acid proteinase inhibitor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000425)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G47550
fragaria_vesca	FvH4_3g00830 FvH4_3g07950 FvH4_3g08020 FvH4_3g34980 FvH4_6g03900 FvH4_6g08240
malus_domestica	MD03G1148300.v1.1 MD04G1217400.v1.1 MD05G1358700.v1.1 MD05G1358900.v1.1 MD05G1359200.v1.1 MD10G1333500.v1.1 MD12G1232100.v1.1
prunus_persica	Prupe.4G008900_v2.0.a1 Prupe.4G009000_v2.0.a1 Prupe.4G009100_v2.0.a1 Prupe.6G215700_v2.0.a1 Prupe.6G335600_v2.0.a1 Prupe.6G335700_v2.0.a1
pyrus_communis	pycom04g19190 pycom05g32440 pycom12g21450 pycom12g21470
rosa_chinensis	RchiOBHm_Chr1g0361291 RchiOBHm_Chr3g0452681 RchiOBHm_Chr3g0455001 RchiOBHm_Chr5g0001051 RchiOBHm_Chr5g0001151 RchiOBHm_Chr5g0010271 RchiOBHm_Chr5g0010621
rosa_laevigata	RLG00000025391 RLG00000025394 RLG00000027728 RLG00000030917 RLG00000031733 RLG00000031766
rosa_multiflora	Rmu_co8350815.1_g000001 Rmu_co8502581.1_g000001 Rmu_sc0000255.1_g000041 Rmu_sc0000818.1_g000015 Rmu_sc0001706.1_g000018 Rmu_sc0001706.1_g000034 Rmu_sc0002150.1_g000001 Rmu_sc0004189.1_g000004 Rmu_sc0014635.1_g000005 Rmu_sc0016264.1_g000005 Rmu_sc0016264.1_g000006 Rmu_sc0021253.1_g000005 Rmu_ssc0000156.1_g000008
rosa_roxburghii	Rroxscaffold_1G00065750 Rroxscaffold_1G00065970 Rroxscaffold_1G00075200 Rroxscaffold_1G00075240 Rroxscaffold_4G00295080 Rroxscaffold_6G00412050 Rroxscaffold_6G00424610
rosa_rugosa	Rorug01G0287800 Rorug02G0642700 Rorug03G0004000 Rorug04G0389300 Rorug04G0449900 Rorug04G0450000 Rorug04G0451200 Rorug05G0127000
rosa_samantha	Rh1AG227300 Rh1AG299200 Rh1BG263000 Rh1CG280500 Rh1DG293000 Rh3AG045000 Rh3AG063500 Rh3BG047300 Rh3BG065400 Rh3CG045800 Rh3CG064300 Rh3DG065500 Rh4CG037800 Rh5AG080900 Rh5BG010600 Rh5BG011000 Rh5BG011100 Rh5BG075500 Rh5CG009200 Rh5CG009700 Rh5CG089100 Rh5CG090700 Rh5DG078200
rosa_wichuraiana	Rw0G000700 Rw0G021870 Rw1G026470 Rw2G026200 Rw3G004990 Rw4G002670 Rw5G000930 Rw5G007490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	214
AciI	CCGC	3 cut(s)	75, 134, 273
AclWI	GGATC	1 cut(s)	263
AfaI	GTAC	1 cut(s)	146
AfiI	CCNNNNNNNGG	3 cut(s)	46, 74, 214
AjnI	CCWGG	2 cut(s)	195, 207
Alw21I	GWGCWC	1 cut(s)	66
Alw44I	GTGCAC	1 cut(s)	62
AlwI	GGATC	1 cut(s)	263
ApaLI	GTGCAC	1 cut(s)	62
ApeKI	GCWGC	2 cut(s)	53, 82
AspLEI	GCGC	1 cut(s)	6
AsuHPI	GGTGA	1 cut(s)	212
BaeGI	GKGCMC	1 cut(s)	66
Bbv12I	GWGCWC	1 cut(s)	66
BbvI	GCAGC	2 cut(s)	40, 94
BciT130I	CCWGG	2 cut(s)	197, 209
BfaI	CTAG	2 cut(s)	236, 376
BisI	GCNGC	2 cut(s)	54, 83
BlsI	GCNGC	2 cut(s)	55, 84
Bme1390I	CCNGG	2 cut(s)	197, 209
BmrFI	CCNGG	2 cut(s)	197, 209
BmsI	GCATC	1 cut(s)	231
BsaJI	CCNNGG	3 cut(s)	68, 160, 196
Bsc4I	CCNNNNNNNGG	3 cut(s)	46, 74, 214
Bse118I	RCCGGY	1 cut(s)	231
Bse1I	ACTGG	1 cut(s)	291
BseBI	CCWGG	2 cut(s)	197, 209
BseDI	CCNNGG	3 cut(s)	68, 160, 196
BseGI	GGATG	1 cut(s)	21
BseLI	CCNNNNNNNGG	3 cut(s)	46, 74, 214
BseNI	ACTGG	1 cut(s)	291
BseRI	GAGGAG	1 cut(s)	8
BseSI	GKGCMC	1 cut(s)	66
BseXI	GCAGC	2 cut(s)	40, 94
BsiHKAI	GWGCWC	1 cut(s)	66
BsiSI	CCGG	1 cut(s)	232
BslI	CCNNNNNNNGG	3 cut(s)	46, 74, 214
Bsp1286I	GDGCHC	1 cut(s)	66
Bsp143I	GATC	2 cut(s)	120, 268
BspACI	CCGC	3 cut(s)	75, 134, 273
BspPI	GGATC	1 cut(s)	263
BsrFI	RCCGGY	1 cut(s)	231
BsrI	ACTGG	1 cut(s)	291
BssAI	RCCGGY	1 cut(s)	231
BssECI	CCNNGG	3 cut(s)	68, 160, 196
BssMI	GATC	2 cut(s)	120, 268
BssT1I	CCWWGG	1 cut(s)	160
Bst2UI	CCWGG	2 cut(s)	197, 209
BstAPI	GCANNNNNTGC	1 cut(s)	131
BstC8I	GCNNGC	2 cut(s)	156, 220
BstDSI	CCRYGG	1 cut(s)	68
BstF5I	GGATG	1 cut(s)	21
BstHHI	GCGC	1 cut(s)	6
BstKTI	GATC	2 cut(s)	123, 271
BstMBI	GATC	2 cut(s)	120, 268
BstMWI	GCNNNNNNNGC	3 cut(s)	12, 131, 337
BstNI	CCWGG	2 cut(s)	197, 209
BstNSI	RCATGY	1 cut(s)	158
BstSCI	CCNGG	2 cut(s)	195, 207
BstSLI	GKGCMC	1 cut(s)	66
BstV1I	GCAGC	2 cut(s)	40, 94
BtgI	CCRYGG	1 cut(s)	68
BtsCI	GGATG	1 cut(s)	21
BtsIMutI	CAGTG	1 cut(s)	298
Cac8I	GCNNGC	2 cut(s)	156, 220
CfoI	GCGC	1 cut(s)	6
Cfr10I	RCCGGY	1 cut(s)	231
Csp6I	GTAC	1 cut(s)	145
CviAII	CATG	2 cut(s)	110, 155
CviJI	RGCY	5 cut(s)	53, 85, 207, 235, 323
CviKI_1	RGCY	5 cut(s)	53, 85, 207, 235, 323
CviQI	GTAC	1 cut(s)	145
DpnI	GATC	2 cut(s)	122, 270
DpnII	GATC	2 cut(s)	120, 268
Eco130I	CCWWGG	1 cut(s)	160
EcoRII	CCWGG	2 cut(s)	195, 207
EcoT14I	CCWWGG	1 cut(s)	160
ErhI	CCWWGG	1 cut(s)	160
FaeI	CATG	2 cut(s)	113, 158
FaiI	YATR	4 cut(s)	89, 111, 156, 300
FatI	CATG	2 cut(s)	109, 154
FauI	CCCGC	1 cut(s)	280
Fnu4HI	GCNGC	2 cut(s)	54, 83
FokI	GGATG	1 cut(s)	8
Fsp4HI	GCNGC	2 cut(s)	54, 83
FspBI	CTAG	2 cut(s)	236, 376
GlaI	GCGC	1 cut(s)	5
GluI	GCNGC	2 cut(s)	54, 83
HapII	CCGG	1 cut(s)	232
HhaI	GCGC	1 cut(s)	6
Hin1II	CATG	2 cut(s)	113, 158
Hin6I	GCGC	1 cut(s)	4
HinP1I	GCGC	1 cut(s)	4
HinfI	GANTC	1 cut(s)	257
HpaII	CCGG	1 cut(s)	232
HphI	GGTGA	1 cut(s)	212
Hpy166II	GTNNAC	1 cut(s)	64
Hpy8I	GTNNAC	1 cut(s)	64
HpyAV	CCTTC	4 cut(s)	40, 50, 116, 313
HpyCH4V	TGCA	1 cut(s)	64
HpyF10VI	GCNNNNNNNGC	3 cut(s)	12, 131, 337
Hsp92II	CATG	2 cut(s)	113, 158
HspAI	GCGC	1 cut(s)	4
Kzo9I	GATC	2 cut(s)	120, 268
LmnI	GCTCC	1 cut(s)	328
LpnPI	CCDG	8 cut(s)	32, 182, 194, 204, 209, 221, 245, 304
Lsp1109I	GCAGC	2 cut(s)	40, 94
LweI	GCATC	1 cut(s)	231
MaeI	CTAG	2 cut(s)	236, 376
MaeIII	GTNAC	1 cut(s)	200
MalI	GATC	2 cut(s)	122, 270
MboI	GATC	2 cut(s)	120, 268
MfeI	CAATTG	1 cut(s)	359
MhlI	GDGCHC	1 cut(s)	66
MluCI	AATT	1 cut(s)	359
MlyI	GAGTC	1 cut(s)	266
MmeI	TCCRAC	1 cut(s)	120
MnlI	CCTC	6 cut(s)	26, 29, 35, 47, 54, 295
MseI	TTAA	1 cut(s)	365
MspA1I	CMGCKG	1 cut(s)	275
MspI	CCGG	1 cut(s)	232
MspR9I	CCNGG	2 cut(s)	197, 209
MunI	CAATTG	1 cut(s)	359
MvaI	CCWGG	2 cut(s)	197, 209
MwoI	GCNNNNNNNGC	3 cut(s)	12, 131, 337
NdeII	GATC	2 cut(s)	120, 268
NlaIII	CATG	2 cut(s)	113, 158
NmuCI	GTSAC	1 cut(s)	200
NspI	RCATGY	1 cut(s)	158
PaeI	GCATGC	1 cut(s)	158
PflMI	CCANNNNNTGG	1 cut(s)	214
PkrI	GCNGC	2 cut(s)	55, 84
PleI	GAGTC	1 cut(s)	265
PpsI	GAGTC	1 cut(s)	265
Psp6I	CCWGG	2 cut(s)	195, 207
PspGI	CCWGG	2 cut(s)	195, 207
RsaI	GTAC	1 cut(s)	146
RsaNI	GTAC	1 cut(s)	145
SaqAI	TTAA	1 cut(s)	365
SatI	GCNGC	2 cut(s)	54, 83
Sau3AI	GATC	2 cut(s)	120, 268
SchI	GAGTC	1 cut(s)	266
ScrFI	CCNGG	2 cut(s)	197, 209
SduI	GDGCHC	1 cut(s)	66
SetI	ASST	3 cut(s)	108, 213, 373
SfaNI	GCATC	1 cut(s)	231
SphI	GCATGC	1 cut(s)	158
Sse9I	AATT	1 cut(s)	359
SsiI	CCGC	3 cut(s)	75, 134, 273
SspMI	CTAG	2 cut(s)	236, 376
StyD4I	CCNGG	2 cut(s)	195, 207
StyI	CCWWGG	1 cut(s)	160
TasI	AATT	1 cut(s)	359
TatI	WGTACW	1 cut(s)	144
Tru1I	TTAA	1 cut(s)	365
Tru9I	TTAA	1 cut(s)	365
TscAI	CASTG	1 cut(s)	298
TseFI	GTSAC	1 cut(s)	200
TseI	GCWGC	2 cut(s)	53, 82
Tsp45I	GTSAC	1 cut(s)	200
TspDTI	ATGAA	3 cut(s)	98, 339, 344
TspRI	CASTG	1 cut(s)	298
Van91I	CCANNNNNTGG	1 cut(s)	214
VneI	GTGCAC	1 cut(s)	62
XceI	RCATGY	1 cut(s)	158
XcmI	CCANNNNNNNNNTGG	1 cut(s)	75
XspI	CTAG	2 cut(s)	236, 376

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.