Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000425

Physical Location & Seq

Genomic Coordinates Forward (+)

47419265 .. 47420545

Gene Structure 1281 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1359200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 699 bp

ATGTTTTTTTATACAAGTGTAAAGCTTAGATTAAAAGCTCAGATTAGCTTAGTACTATCTGGTGGTTGTGGTTTTTGTCAACTAACTCCAATGGAGATAAGTTTTGACCTTGTATACCAAAACCCTACTGACAGCATATATATATATGTTTGCTATGAGCTGCTAAGAGTGCTGACTCCAATAGCCGAAATCCCTCCTTACATTTCTGTGTTTTCCATAACCAAAAACCCTGTCAAGAACATTTCTACAAAATATAAACCTTCCAAAACACTAACATTCTGGTGGTGGGTTGTAGATTCGCTTGCTCAGTTCCCCTCCTCCGCCGTTGATGCAGCCAAAAAAGCCGGCGAGGTGCATTTGGTCACGGAGACTGATAACCGACGGGGCCGTATATGTGGCGGTTGGTGGCCCATAGAGAACATCAGCGACCCCCATGTGAAGGAGATTGCAGAGTTTGCGGTGTCGGAGTACAACGAGCAAGCCAAAGGCCAGAACAAGCTGGCGTTTGAGAGGGTCGTCCGGGGCGACAGACAGCTGGTGGCGGGCATGAACTACCGGCTTGTCATTTCCGCTAAGAACAAGTCCGTGGCTGATCCCAATGATGCCACTCCCGCGGAATACGAGGGTATTGTGTGGGAGAGGACTAGGCTGCATTTTAAGCAGTTGACATCATTTCATCGATTGTCAAACCCTAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002020 GO:0003674 GO:0004857 GO:0004866 GO:0004869 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005623 GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0009892 GO:0009987 GO:0010029 GO:0010030 GO:0010466 GO:0010605 GO:0010951 GO:0019222 GO:0019899 GO:0030162 GO:0030234 GO:0030312 GO:0030414 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0033554 GO:0034605 GO:0043086 GO:0044092 GO:0044464 GO:0045861 GO:0048518 GO:0048519 GO:0048523 GO:0048580 GO:0048582 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0051716 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0065007 GO:0065009 GO:0071944 GO:0080090 GO:0098772 GO:1900140 GO:2000026 GO:2000116 GO:2000117

KEGG Pathways

Metabolic & Signaling

ko04970 map04970

Pfam Domains

Protein Families

SQAPI

Protein Analysis

233

Amino Acids

26.4

Weight (kDa)

8.8

Isoelectric Point (pI)

28.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cystatin	PF00031	137 - 198	6.8e-13	Cystatin domain
SQAPI	PF16845	138 - 227	7e-28	Aspartic acid proteinase inhibitor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0000425)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G47550
fragaria_vesca	FvH4_3g00830 FvH4_3g07950 FvH4_3g08020 FvH4_3g34980 FvH4_6g03900 FvH4_6g08240
malus_domestica	MD03G1148300.v1.1 MD04G1217400.v1.1 MD05G1358700.v1.1 MD05G1358900.v1.1 MD05G1359200.v1.1 MD10G1333500.v1.1 MD12G1232100.v1.1
prunus_persica	Prupe.4G008900_v2.0.a1 Prupe.4G009000_v2.0.a1 Prupe.4G009100_v2.0.a1 Prupe.6G215700_v2.0.a1 Prupe.6G335600_v2.0.a1 Prupe.6G335700_v2.0.a1
pyrus_communis	pycom04g19190 pycom05g32440 pycom12g21450 pycom12g21470
rosa_chinensis	RchiOBHm_Chr1g0361291 RchiOBHm_Chr3g0452681 RchiOBHm_Chr3g0455001 RchiOBHm_Chr5g0001051 RchiOBHm_Chr5g0001151 RchiOBHm_Chr5g0010271 RchiOBHm_Chr5g0010621
rosa_laevigata	RLG00000025391 RLG00000025394 RLG00000027728 RLG00000030917 RLG00000031733 RLG00000031766
rosa_multiflora	Rmu_co8350815.1_g000001 Rmu_co8502581.1_g000001 Rmu_sc0000255.1_g000041 Rmu_sc0000818.1_g000015 Rmu_sc0001706.1_g000018 Rmu_sc0001706.1_g000034 Rmu_sc0002150.1_g000001 Rmu_sc0004189.1_g000004 Rmu_sc0014635.1_g000005 Rmu_sc0016264.1_g000005 Rmu_sc0016264.1_g000006 Rmu_sc0021253.1_g000005 Rmu_ssc0000156.1_g000008
rosa_roxburghii	Rroxscaffold_1G00065750 Rroxscaffold_1G00065970 Rroxscaffold_1G00075200 Rroxscaffold_1G00075240 Rroxscaffold_4G00295080 Rroxscaffold_6G00412050 Rroxscaffold_6G00424610
rosa_rugosa	Rorug01G0287800 Rorug02G0642700 Rorug03G0004000 Rorug04G0389300 Rorug04G0449900 Rorug04G0450000 Rorug04G0451200 Rorug05G0127000
rosa_samantha	Rh1AG227300 Rh1AG299200 Rh1BG263000 Rh1CG280500 Rh1DG293000 Rh3AG045000 Rh3AG063500 Rh3BG047300 Rh3BG065400 Rh3CG045800 Rh3CG064300 Rh3DG065500 Rh4CG037800 Rh5AG080900 Rh5BG010600 Rh5BG011000 Rh5BG011100 Rh5BG075500 Rh5CG009200 Rh5CG009700 Rh5CG089100 Rh5CG090700 Rh5DG078200
rosa_wichuraiana	Rw0G000700 Rw0G021870 Rw1G026470 Rw2G026200 Rw3G004990 Rw4G002670 Rw5G000930 Rw5G007490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	114
AccII	CGCG	1 cut(s)	614
AciI	CCGC	7 cut(s)	321, 399, 458, 542, 570, 612, 614
AclWI	GGATC	1 cut(s)	587
AfaI	GTAC	2 cut(s)	54, 470
AfiI	CCNNNNNNNGG	1 cut(s)	439
AluBI	AGCT	6 cut(s)	25, 38, 48, 160, 499, 535
AluI	AGCT	6 cut(s)	25, 38, 48, 160, 499, 535
Alw26I	GTCTC	1 cut(s)	362
AlwI	GGATC	1 cut(s)	587
AoxI	GGCC	3 cut(s)	385, 407, 487
ApeKI	GCWGC	3 cut(s)	160, 332, 649
ArsI	GACNNNNNNTTYG	2 cut(s)	216, 248
AspS9I	GGNCC	2 cut(s)	385, 408
AsuC2I	CCSGG	1 cut(s)	521
BbvI	GCAGC	3 cut(s)	147, 344, 636
BceAI	ACGGC	2 cut(s)	308, 372
BcnI	CCSGG	1 cut(s)	521
BcoDI	GTCTC	1 cut(s)	362
BfaI	CTAG	2 cut(s)	645, 697
BisI	GCNGC	3 cut(s)	161, 333, 650
BlsI	GCNGC	3 cut(s)	162, 334, 651
BmcAI	AGTACT	1 cut(s)	54
Bme1390I	CCNGG	1 cut(s)	521
BmgT120I	GGNCC	2 cut(s)	385, 408
BmiI	GGNNCC	1 cut(s)	386
BmrFI	CCNGG	1 cut(s)	521
BmsI	GCATC	2 cut(s)	319, 592
BpuMI	CCSGG	1 cut(s)	521
Bsa29I	ATCGAT	1 cut(s)	679
BsaJI	CCNNGG	3 cut(s)	520, 585, 612
Bsc4I	CCNNNNNNNGG	1 cut(s)	439
Bse118I	RCCGGY	2 cut(s)	344, 555
BseCI	ATCGAT	1 cut(s)	679
BseDI	CCNNGG	3 cut(s)	520, 585, 612
BseLI	CCNNNNNNNGG	1 cut(s)	439
BseMII	CTCAG	2 cut(s)	53, 320
BseRI	GAGGAG	1 cut(s)	307
BseXI	GCAGC	3 cut(s)	147, 344, 636
Bsh1236I	CGCG	1 cut(s)	614
BshFI	GGCC	3 cut(s)	387, 409, 489
BshVI	ATCGAT	1 cut(s)	679
BsiSI	CCGG	3 cut(s)	345, 520, 556
BslI	CCNNNNNNNGG	1 cut(s)	439
BsmAI	GTCTC	1 cut(s)	362
BsnI	GGCC	3 cut(s)	387, 409, 489
Bsp143I	GATC	1 cut(s)	592
BspACI	CCGC	7 cut(s)	321, 399, 458, 542, 570, 612, 614
BspANI	GGCC	3 cut(s)	387, 409, 489
BspCNI	CTCAG	2 cut(s)	52, 319
BspDI	ATCGAT	1 cut(s)	679
BspFNI	CGCG	1 cut(s)	614
BspLI	GGNNCC	1 cut(s)	386
BspPI	GGATC	1 cut(s)	587
BsrFI	RCCGGY	2 cut(s)	344, 555
BssAI	RCCGGY	2 cut(s)	344, 555
BssECI	CCNNGG	3 cut(s)	520, 585, 612
BssMI	GATC	1 cut(s)	592
BssNAI	GTATAC	1 cut(s)	115
Bst1107I	GTATAC	1 cut(s)	115
BstAPI	GCANNNNNTGC	1 cut(s)	455
BstC8I	GCNNGC	5 cut(s)	303, 346, 480, 501, 544
BstDEI	CTNAG	6 cut(s)	26, 39, 49, 164, 306, 573
BstDSI	CCRYGG	2 cut(s)	585, 612
BstFNI	CGCG	1 cut(s)	614
BstKTI	GATC	1 cut(s)	595
BstMAI	GTCTC	1 cut(s)	362
BstMBI	GATC	1 cut(s)	592
BstMWI	GCNNNNNNNGC	6 cut(s)	169, 329, 341, 455, 611, 658
BstSCI	CCNGG	1 cut(s)	519
BstUI	CGCG	1 cut(s)	614
BstV1I	GCAGC	3 cut(s)	147, 344, 636
BstZ17I	GTATAC	1 cut(s)	115
Bsu15I	ATCGAT	1 cut(s)	679
BsuRI	GGCC	3 cut(s)	387, 409, 489
BsuTUI	ATCGAT	1 cut(s)	679
BtgI	CCRYGG	2 cut(s)	585, 612
Cac8I	GCNNGC	5 cut(s)	303, 346, 480, 501, 544
Cfr10I	RCCGGY	2 cut(s)	344, 555
Cfr13I	GGNCC	2 cut(s)	385, 408
Cfr42I	CCGCGG	1 cut(s)	615
ClaI	ATCGAT	1 cut(s)	679
Csp6I	GTAC	2 cut(s)	53, 469
CviAII	CATG	2 cut(s)	434, 547
CviQI	GTAC	2 cut(s)	53, 469
DdeI	CTNAG	6 cut(s)	26, 39, 49, 164, 306, 573
DpnI	GATC	1 cut(s)	594
DpnII	GATC	1 cut(s)	592
EciI	GGCGGA	1 cut(s)	310
FaeI	CATG	2 cut(s)	437, 550
FatI	CATG	2 cut(s)	433, 546
FauI	CCCGC	2 cut(s)	535, 619
FblI	GTMKAC	1 cut(s)	114
Fnu4HI	GCNGC	3 cut(s)	161, 333, 650
Fsp4HI	GCNGC	3 cut(s)	161, 333, 650
FspBI	CTAG	2 cut(s)	645, 697
GluI	GCNGC	3 cut(s)	161, 333, 650
HaeIII	GGCC	3 cut(s)	387, 409, 489
HapII	CCGG	3 cut(s)	345, 520, 556
Hin1II	CATG	2 cut(s)	437, 550
HincII	GTYRAC	2 cut(s)	80, 666
HindII	GTYRAC	2 cut(s)	80, 666
HindIII	AAGCTT	1 cut(s)	23
HinfI	GANTC	2 cut(s)	175, 296
HpaII	CCGG	3 cut(s)	345, 520, 556
Hpy166II	GTNNAC	3 cut(s)	80, 115, 666
Hpy188I	TCNGA	2 cut(s)	42, 466
Hpy188III	TCNNGA	1 cut(s)	235
Hpy8I	GTNNAC	3 cut(s)	80, 115, 666
Hpy99I	CGWCG	1 cut(s)	384
HpyAV	CCTTC	2 cut(s)	270, 433
HpyCH4V	TGCA	4 cut(s)	332, 355, 449, 652
HpyF10VI	GCNNNNNNNGC	6 cut(s)	169, 329, 341, 455, 611, 658
HpyF3I	CTNAG	6 cut(s)	26, 39, 49, 164, 306, 573
Hsp92II	CATG	2 cut(s)	437, 550
KroI	GCCGGC	1 cut(s)	344
KroNI	GCCGGC	1 cut(s)	346
KspI	CCGCGG	1 cut(s)	615
Kzo9I	GATC	1 cut(s)	592
LpnPI	CCDG	9 cut(s)	45, 243, 265, 358, 485, 503, 521, 533, 569
Lsp1109I	GCAGC	3 cut(s)	147, 344, 636
LweI	GCATC	2 cut(s)	319, 592
MaeI	CTAG	2 cut(s)	645, 697
MaeIII	GTNAC	1 cut(s)	361
MalI	GATC	1 cut(s)	594
MboI	GATC	1 cut(s)	592
MlyI	GAGTC	1 cut(s)	169
MmeI	TCCRAC	1 cut(s)	444
MnlI	CCTC	7 cut(s)	204, 325, 328, 343, 504, 616, 633
MroNI	GCCGGC	1 cut(s)	344
MseI	TTAA	2 cut(s)	32, 657
MslI	CAYNNNNRTG	2 cut(s)	206, 280
MspA1I	CMGCKG	2 cut(s)	535, 614
MspI	CCGG	3 cut(s)	345, 520, 556
MspR9I	CCNGG	1 cut(s)	521
MvnI	CGCG	1 cut(s)	614
MwoI	GCNNNNNNNGC	6 cut(s)	169, 329, 341, 455, 611, 658
NaeI	GCCGGC	1 cut(s)	346
NciI	CCSGG	1 cut(s)	521
NdeII	GATC	1 cut(s)	592
NgoMIV	GCCGGC	1 cut(s)	344
NlaIII	CATG	2 cut(s)	437, 550
NlaIV	GGNNCC	1 cut(s)	386
NmuCI	GTSAC	1 cut(s)	361
PcsI	WCGNNNNNNNCGW	1 cut(s)	522
PdiI	GCCGGC	1 cut(s)	346
PfeI	GAWTC	1 cut(s)	296
PkrI	GCNGC	3 cut(s)	162, 334, 651
PleI	GAGTC	1 cut(s)	169
PpsI	GAGTC	1 cut(s)	169
PspN4I	GGNNCC	1 cut(s)	386
PspPI	GGNCC	2 cut(s)	385, 408
PvuII	CAGCTG	1 cut(s)	535
RsaI	GTAC	2 cut(s)	54, 470
RsaNI	GTAC	2 cut(s)	53, 469
RseI	CAYNNNNRTG	2 cut(s)	206, 280
SacII	CCGCGG	1 cut(s)	615
SaqAI	TTAA	2 cut(s)	32, 657
SatI	GCNGC	3 cut(s)	161, 333, 650
Sau3AI	GATC	1 cut(s)	592
Sau96I	GGNCC	2 cut(s)	385, 408
ScaI	AGTACT	1 cut(s)	54
SchI	GAGTC	1 cut(s)	169
ScrFI	CCNGG	1 cut(s)	521
SetI	ASST	9 cut(s)	27, 40, 50, 111, 162, 262, 354, 501, 537
SfaNI	GCATC	2 cut(s)	319, 592
Sfr303I	CCGCGG	1 cut(s)	615
SgrBI	CCGCGG	1 cut(s)	615
SmiMI	CAYNNNNRTG	2 cut(s)	206, 280
SsiI	CCGC	7 cut(s)	321, 399, 458, 542, 570, 612, 614
SspMI	CTAG	2 cut(s)	645, 697
StyD4I	CCNGG	1 cut(s)	519
TaqI	TCGA	1 cut(s)	679
TatI	WGTACW	2 cut(s)	52, 468
TfiI	GAWTC	1 cut(s)	296
Tru1I	TTAA	2 cut(s)	32, 657
Tru9I	TTAA	2 cut(s)	32, 657
TseFI	GTSAC	1 cut(s)	361
TseI	GCWGC	3 cut(s)	160, 332, 649
Tsp45I	GTSAC	1 cut(s)	361
TspDTI	ATGAA	2 cut(s)	563, 665
TspGWI	ACGGA	2 cut(s)	380, 574
XmiI	GTMKAC	1 cut(s)	114
XspI	CTAG	2 cut(s)	645, 697
ZrmI	AGTACT	1 cut(s)	54

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.