Belongs to the cystatin family. Phytocystatin subfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 264 |
| AccII | CGCG | 2 cut(s) | 164, 278 |
| AciI | CCGC | 3 cut(s) | 164, 251, 278 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 110, 130 |
| AflIII | ACRYGT | 1 cut(s) | 10 |
| AluBI | AGCT | 3 cut(s) | 184, 208, 268 |
| AluI | AGCT | 3 cut(s) | 184, 208, 268 |
| Alw21I | GWGCWC | 1 cut(s) | 186 |
| Alw26I | GTCTC | 1 cut(s) | 181 |
| AoxI | GGCC | 1 cut(s) | 52 |
| AspLEI | GCGC | 1 cut(s) | 116 |
| AsuHPI | GGTGA | 4 cut(s) | 56, 65, 113, 245 |
| BanII | GRGCYC | 1 cut(s) | 186 |
| Bbv12I | GWGCWC | 1 cut(s) | 186 |
| BceAI | ACGGC | 1 cut(s) | 127 |
| BcoDI | GTCTC | 1 cut(s) | 181 |
| BfoI | RGCGCY | 1 cut(s) | 117 |
| BsaBI | GATNNNNATC | 2 cut(s) | 99, 158 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 290, 320 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 110, 130 |
| Bse118I | RCCGGY | 1 cut(s) | 50 |
| Bse8I | GATNNNNATC | 2 cut(s) | 99, 158 |
| BseGI | GGATG | 2 cut(s) | 135, 292 |
| BseJI | GATNNNNATC | 2 cut(s) | 99, 158 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 110, 130 |
| BseMII | CTCAG | 1 cut(s) | 59 |
| Bsh1236I | CGCG | 2 cut(s) | 164, 278 |
| BshFI | GGCC | 1 cut(s) | 54 |
| BsiHKAI | GWGCWC | 1 cut(s) | 186 |
| BsiSI | CCGG | 1 cut(s) | 51 |
| BslFI | GGGAC | 1 cut(s) | 267 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 110, 130 |
| BsmAI | GTCTC | 1 cut(s) | 181 |
| BsmFI | GGGAC | 1 cut(s) | 267 |
| BsnI | GGCC | 1 cut(s) | 54 |
| Bsp1286I | GDGCHC | 1 cut(s) | 186 |
| Bsp143I | GATC | 1 cut(s) | 159 |
| BspACI | CCGC | 3 cut(s) | 164, 251, 278 |
| BspANI | GGCC | 1 cut(s) | 54 |
| BspCNI | CTCAG | 1 cut(s) | 60 |
| BspFNI | CGCG | 2 cut(s) | 164, 278 |
| BspHI | TCATGA | 1 cut(s) | 376 |
| BsrFI | RCCGGY | 1 cut(s) | 50 |
| BssAI | RCCGGY | 1 cut(s) | 50 |
| BssMI | GATC | 1 cut(s) | 159 |
| Bst4CI | ACNGT | 1 cut(s) | 313 |
| BstC8I | GCNNGC | 2 cut(s) | 52, 84 |
| BstDEI | CTNAG | 1 cut(s) | 68 |
| BstF5I | GGATG | 2 cut(s) | 135, 292 |
| BstFNI | CGCG | 2 cut(s) | 164, 278 |
| BstH2I | RGCGCY | 1 cut(s) | 117 |
| BstHHI | GCGC | 1 cut(s) | 116 |
| BstKTI | GATC | 1 cut(s) | 162 |
| BstMAI | GTCTC | 1 cut(s) | 181 |
| BstMBI | GATC | 1 cut(s) | 159 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 170 |
| BstUI | CGCG | 2 cut(s) | 164, 278 |
| BsuRI | GGCC | 1 cut(s) | 54 |
| BtsCI | GGATG | 2 cut(s) | 135, 292 |
| BtsIMutI | CAGTG | 1 cut(s) | 309 |
| Cac8I | GCNNGC | 2 cut(s) | 52, 84 |
| CciI | TCATGA | 1 cut(s) | 376 |
| CfoI | GCGC | 1 cut(s) | 116 |
| Cfr10I | RCCGGY | 1 cut(s) | 50 |
| CviAII | CATG | 2 cut(s) | 149, 377 |
| CviJI | RGCY | 5 cut(s) | 54, 184, 208, 268, 349 |
| CviKI_1 | RGCY | 5 cut(s) | 54, 184, 208, 268, 349 |
| DdeI | CTNAG | 1 cut(s) | 68 |
| DpnI | GATC | 1 cut(s) | 161 |
| DpnII | GATC | 1 cut(s) | 159 |
| Ecl136II | GAGCTC | 1 cut(s) | 184 |
| Eco24I | GRGCYC | 1 cut(s) | 186 |
| Eco53kI | GAGCTC | 1 cut(s) | 184 |
| EcoICRI | GAGCTC | 1 cut(s) | 184 |
| EcoT38I | GRGCYC | 1 cut(s) | 186 |
| FaeI | CATG | 2 cut(s) | 152, 380 |
| FaiI | YATR | 4 cut(s) | 150, 264, 321, 378 |
| FaqI | GGGAC | 1 cut(s) | 267 |
| FatI | CATG | 2 cut(s) | 148, 376 |
| FauI | CCCGC | 1 cut(s) | 244 |
| FokI | GGATG | 2 cut(s) | 122, 299 |
| FriOI | GRGCYC | 1 cut(s) | 186 |
| GlaI | GCGC | 1 cut(s) | 115 |
| HaeII | RGCGCY | 1 cut(s) | 117 |
| HaeIII | GGCC | 1 cut(s) | 54 |
| HapII | CCGG | 1 cut(s) | 51 |
| HhaI | GCGC | 1 cut(s) | 116 |
| Hin1II | CATG | 2 cut(s) | 152, 380 |
| Hin6I | GCGC | 1 cut(s) | 114 |
| HinP1I | GCGC | 1 cut(s) | 114 |
| HindIII | AAGCTT | 1 cut(s) | 266 |
| HinfI | GANTC | 3 cut(s) | 70, 238, 290 |
| HpaII | CCGG | 1 cut(s) | 51 |
| HphI | GGTGA | 4 cut(s) | 56, 65, 113, 245 |
| Hpy188I | TCNGA | 2 cut(s) | 181, 219 |
| Hpy188III | TCNNGA | 1 cut(s) | 377 |
| Hpy99I | CGWCG | 1 cut(s) | 278 |
| HpyAV | CCTTC | 2 cut(s) | 53, 334 |
| HpyCH4III | ACNGT | 1 cut(s) | 313 |
| HpyCH4IV | ACGT | 1 cut(s) | 10 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 170 |
| HpyF3I | CTNAG | 1 cut(s) | 68 |
| HpySE526I | ACGT | 1 cut(s) | 10 |
| Hsp92II | CATG | 2 cut(s) | 152, 380 |
| HspAI | GCGC | 1 cut(s) | 114 |
| KroI | GCCGGC | 1 cut(s) | 50 |
| KroNI | GCCGGC | 1 cut(s) | 52 |
| Kzo9I | GATC | 1 cut(s) | 159 |
| LpnPI | CCDG | 6 cut(s) | 64, 80, 92, 194, 227, 319 |
| MaeII | ACGT | 1 cut(s) | 10 |
| MaeIII | GTNAC | 1 cut(s) | 71 |
| MalI | GATC | 1 cut(s) | 161 |
| MboI | GATC | 1 cut(s) | 159 |
| MboII | GAAGA | 1 cut(s) | 214 |
| MhlI | GDGCHC | 1 cut(s) | 186 |
| MluCI | AATT | 3 cut(s) | 259, 316, 362 |
| MlyI | GAGTC | 3 cut(s) | 79, 247, 299 |
| MroNI | GCCGGC | 1 cut(s) | 50 |
| MseI | TTAA | 2 cut(s) | 129, 384 |
| MspI | CCGG | 1 cut(s) | 51 |
| MvnI | CGCG | 2 cut(s) | 164, 278 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 170 |
| NaeI | GCCGGC | 1 cut(s) | 52 |
| NdeII | GATC | 1 cut(s) | 159 |
| NgoMIV | GCCGGC | 1 cut(s) | 50 |
| NlaIII | CATG | 2 cut(s) | 152, 380 |
| NmuCI | GTSAC | 1 cut(s) | 71 |
| PagI | TCATGA | 1 cut(s) | 376 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 90 |
| PdiI | GCCGGC | 1 cut(s) | 52 |
| PleI | GAGTC | 3 cut(s) | 78, 246, 298 |
| PpsI | GAGTC | 3 cut(s) | 78, 246, 298 |
| PsiI | TTATAA | 1 cut(s) | 264 |
| Psp124BI | GAGCTC | 1 cut(s) | 186 |
| SacI | GAGCTC | 1 cut(s) | 186 |
| SaqAI | TTAA | 2 cut(s) | 129, 384 |
| Sau3AI | GATC | 1 cut(s) | 159 |
| SchI | GAGTC | 3 cut(s) | 79, 247, 299 |
| SduI | GDGCHC | 1 cut(s) | 186 |
| Sse9I | AATT | 3 cut(s) | 259, 316, 362 |
| SsiI | CCGC | 3 cut(s) | 164, 251, 278 |
| SstI | GAGCTC | 1 cut(s) | 186 |
| TaaI | ACNGT | 1 cut(s) | 313 |
| TaiI | ACGT | 1 cut(s) | 13 |
| TaqI | TCGA | 2 cut(s) | 24, 229 |
| TasI | AATT | 3 cut(s) | 259, 316, 362 |
| Tru1I | TTAA | 2 cut(s) | 129, 384 |
| Tru9I | TTAA | 2 cut(s) | 129, 384 |
| TscAI | CASTG | 1 cut(s) | 316 |
| TseFI | GTSAC | 1 cut(s) | 71 |
| Tsp45I | GTSAC | 1 cut(s) | 71 |
| TspDTI | ATGAA | 1 cut(s) | 365 |
| TspGWI | ACGGA | 1 cut(s) | 214 |
| TspRI | CASTG | 1 cut(s) | 316 |