Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000425

Physical Location & Seq

Genomic Coordinates Forward (+)

47393276 .. 47393743

Gene Structure 468 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1358700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 468 bp

ATGCATGCCATCACTTGTCACTTGTGGAGTGCAACTGTGTCAGTCAGTCGAAGAGAAGTTAAATCAACGGTCACCATGCGCCCTCAGTGCCTCCTCCTTCTTGTTGCCCTCATCCTTCCTCTGGCCGCCGCCACCAGCACAGTCCAGATCGGCGGTTGGAAACCCATTGAGGACGTCAGCGACCCCGAGGTGCTAGAGGCCGCAGAGTTCGCTGTGTCGGAGTACAACACGAAGCAAGCAAAGGCTCAGAACAAGTTGGTGTTTGAGACAGTGCTCCGGGGTGAGACGCAACTCGTTGCGGGCACGAACTATAAGCTTTTCATTTCGGCAAAAAATGAGTCGTCGGTGGCAAATCCCACCAGGGTTATCGGCACCGCCGCCGCAAATAATTATGAGGTCTTCGTCTATGACAGGGCTTGGGAGCATCACAAGGAATTGGTCTACTTTCATCGTTCGAAAAAAAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002020 GO:0003674 GO:0004857 GO:0004866 GO:0004869 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005623 GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0009892 GO:0009987 GO:0010029 GO:0010030 GO:0010466 GO:0010605 GO:0010951 GO:0019222 GO:0019899 GO:0030162 GO:0030234 GO:0030312 GO:0030414 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0033554 GO:0034605 GO:0043086 GO:0044092 GO:0044464 GO:0045861 GO:0048518 GO:0048519 GO:0048523 GO:0048580 GO:0048582 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0051716 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0065007 GO:0065009 GO:0071944 GO:0080090 GO:0098772 GO:1900140 GO:2000026 GO:2000116 GO:2000117

KEGG Pathways

Metabolic & Signaling

ko04970 map04970

Pfam Domains

Protein Families

SQAPI

Protein Analysis

156

Amino Acids

17.28

Weight (kDa)

8.55

Isoelectric Point (pI)

39.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cystatin	PF00031	51 - 116	3.3e-12	Cystatin domain
SQAPI	PF16845	56 - 150	2.8e-21	Aspartic acid proteinase inhibitor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000425)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G47550
fragaria_vesca	FvH4_3g00830 FvH4_3g07950 FvH4_3g08020 FvH4_3g34980 FvH4_6g03900 FvH4_6g08240
malus_domestica	MD03G1148300.v1.1 MD04G1217400.v1.1 MD05G1358700.v1.1 MD05G1358900.v1.1 MD05G1359200.v1.1 MD10G1333500.v1.1 MD12G1232100.v1.1
prunus_persica	Prupe.4G008900_v2.0.a1 Prupe.4G009000_v2.0.a1 Prupe.4G009100_v2.0.a1 Prupe.6G215700_v2.0.a1 Prupe.6G335600_v2.0.a1 Prupe.6G335700_v2.0.a1
pyrus_communis	pycom04g19190 pycom05g32440 pycom12g21450 pycom12g21470
rosa_chinensis	RchiOBHm_Chr1g0361291 RchiOBHm_Chr3g0452681 RchiOBHm_Chr3g0455001 RchiOBHm_Chr5g0001051 RchiOBHm_Chr5g0001151 RchiOBHm_Chr5g0010271 RchiOBHm_Chr5g0010621
rosa_laevigata	RLG00000025391 RLG00000025394 RLG00000027728 RLG00000030917 RLG00000031733 RLG00000031766
rosa_multiflora	Rmu_co8350815.1_g000001 Rmu_co8502581.1_g000001 Rmu_sc0000255.1_g000041 Rmu_sc0000818.1_g000015 Rmu_sc0001706.1_g000018 Rmu_sc0001706.1_g000034 Rmu_sc0002150.1_g000001 Rmu_sc0004189.1_g000004 Rmu_sc0014635.1_g000005 Rmu_sc0016264.1_g000005 Rmu_sc0016264.1_g000006 Rmu_sc0021253.1_g000005 Rmu_ssc0000156.1_g000008
rosa_roxburghii	Rroxscaffold_1G00065750 Rroxscaffold_1G00065970 Rroxscaffold_1G00075200 Rroxscaffold_1G00075240 Rroxscaffold_4G00295080 Rroxscaffold_6G00412050 Rroxscaffold_6G00424610
rosa_rugosa	Rorug01G0287800 Rorug02G0642700 Rorug03G0004000 Rorug04G0389300 Rorug04G0449900 Rorug04G0450000 Rorug04G0451200 Rorug05G0127000
rosa_samantha	Rh1AG227300 Rh1AG299200 Rh1BG263000 Rh1CG280500 Rh1DG293000 Rh3AG045000 Rh3AG063500 Rh3BG047300 Rh3BG065400 Rh3CG045800 Rh3CG064300 Rh3DG065500 Rh4CG037800 Rh5AG080900 Rh5BG010600 Rh5BG011000 Rh5BG011100 Rh5BG075500 Rh5CG009200 Rh5CG009700 Rh5CG089100 Rh5CG090700 Rh5DG078200
rosa_wichuraiana	Rw0G000700 Rw0G021870 Rw1G026470 Rw2G026200 Rw3G004990 Rw4G002670 Rw5G000930 Rw5G007490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	177
AccB1I	GGYRCC	1 cut(s)	371
AccI	GTMKAC	1 cut(s)	441
AciI	CCGC	8 cut(s)	126, 129, 153, 201, 299, 375, 378, 381
AcoI	YGGCCR	1 cut(s)	123
AcyI	GRCGYC	1 cut(s)	174
AfaI	GTAC	1 cut(s)	224
AfiI	CCNNNNNNNGG	1 cut(s)	121
AjnI	CCWGG	1 cut(s)	359
AluBI	AGCT	1 cut(s)	316
AluI	AGCT	1 cut(s)	316
Alw21I	GWGCWC	1 cut(s)	276
Alw26I	GTCTC	2 cut(s)	260, 278
Ama87I	CYCGRG	1 cut(s)	185
AoxI	GGCC	2 cut(s)	123, 198
ArsI	GACNNNNNNTTYG	2 cut(s)	323, 355
AspLEI	GCGC	1 cut(s)	81
AsuC2I	CCSGG	1 cut(s)	278
AsuHPI	GGTGA	2 cut(s)	64, 293
AsuII	TTCGAA	1 cut(s)	455
AvaI	CYCGRG	1 cut(s)	185
BaeGI	GKGCMC	1 cut(s)	305
BanI	GGYRCC	1 cut(s)	371
BbsI	GAAGAC	1 cut(s)	391
Bbv12I	GWGCWC	1 cut(s)	276
BccI	CCATC	1 cut(s)	17
BciT130I	CCWGG	1 cut(s)	361
BcnI	CCSGG	1 cut(s)	278
BcoDI	GTCTC	2 cut(s)	260, 278
BfaI	CTAG	1 cut(s)	194
BisI	GCNGC	5 cut(s)	126, 129, 201, 378, 381
BlsI	GCNGC	5 cut(s)	127, 130, 202, 379, 382
Bme1390I	CCNGG	2 cut(s)	278, 361
BmeT110I	CYCGRG	1 cut(s)	185
BmiI	GGNNCC	1 cut(s)	373
BmrFI	CCNGG	2 cut(s)	278, 361
BmsI	GCATC	1 cut(s)	433
BpiI	GAAGAC	1 cut(s)	391
Bpu14I	TTCGAA	1 cut(s)	455
BpuMI	CCSGG	1 cut(s)	278
BsaHI	GRCGYC	1 cut(s)	174
BsaJI	CCNNGG	3 cut(s)	186, 277, 360
Bsc4I	CCNNNNNNNGG	1 cut(s)	121
BseBI	CCWGG	1 cut(s)	361
BseDI	CCNNGG	3 cut(s)	186, 277, 360
BseGI	GGATG	1 cut(s)	111
BseLI	CCNNNNNNNGG	1 cut(s)	121
BseMII	CTCAG	2 cut(s)	98, 260
BseRI	GAGGAG	1 cut(s)	83
BseSI	GKGCMC	1 cut(s)	305
BshFI	GGCC	2 cut(s)	125, 200
BshNI	GGYRCC	1 cut(s)	371
BsiHKAI	GWGCWC	1 cut(s)	276
BsiHKCI	CYCGRG	1 cut(s)	185
BsiSI	CCGG	1 cut(s)	277
BslI	CCNNNNNNNGG	1 cut(s)	121
BsmAI	GTCTC	2 cut(s)	260, 278
BsmBI	CGTCTC	1 cut(s)	278
BsnI	GGCC	2 cut(s)	125, 200
BsoBI	CYCGRG	1 cut(s)	185
Bsp119I	TTCGAA	1 cut(s)	455
Bsp1286I	GDGCHC	2 cut(s)	276, 305
Bsp143I	GATC	1 cut(s)	147
BspACI	CCGC	8 cut(s)	126, 129, 153, 201, 299, 375, 378, 381
BspANI	GGCC	2 cut(s)	125, 200
BspCNI	CTCAG	2 cut(s)	97, 259
BspLI	GGNNCC	1 cut(s)	373
BspT104I	TTCGAA	1 cut(s)	455
BspT107I	GGYRCC	1 cut(s)	371
BssECI	CCNNGG	3 cut(s)	186, 277, 360
BssMI	GATC	1 cut(s)	147
BssNI	GRCGYC	1 cut(s)	174
Bst2UI	CCWGG	1 cut(s)	361
Bst4CI	ACNGT	4 cut(s)	37, 70, 142, 271
Bst6I	CTCTTC	1 cut(s)	46
BstACI	GRCGYC	1 cut(s)	174
BstBI	TTCGAA	1 cut(s)	455
BstC8I	GCNNGC	3 cut(s)	6, 237, 301
BstDEI	CTNAG	2 cut(s)	84, 246
BstEII	GGTNACC	1 cut(s)	70
BstF5I	GGATG	1 cut(s)	111
BstHHI	GCGC	1 cut(s)	81
BstKTI	GATC	1 cut(s)	150
BstMAI	GTCTC	2 cut(s)	260, 278
BstMBI	GATC	1 cut(s)	147
BstMWI	GCNNNNNNNGC	2 cut(s)	87, 209
BstNI	CCWGG	1 cut(s)	361
BstNSI	RCATGY	1 cut(s)	8
BstPI	GGTNACC	1 cut(s)	70
BstSCI	CCNGG	2 cut(s)	276, 359
BstSLI	GKGCMC	1 cut(s)	305
BstV2I	GAAGAC	1 cut(s)	391
BsuRI	GGCC	2 cut(s)	125, 200
BtsCI	GGATG	1 cut(s)	111
BtsIMutI	CAGTG	2 cut(s)	92, 276
Cac8I	GCNNGC	3 cut(s)	6, 237, 301
CfoI	GCGC	1 cut(s)	81
CseI	GACGC	1 cut(s)	295
Csp6I	GTAC	1 cut(s)	223
CviAII	CATG	2 cut(s)	5, 76
CviJI	RGCY	5 cut(s)	125, 200, 245, 316, 416
CviKI_1	RGCY	5 cut(s)	125, 200, 245, 316, 416
CviQI	GTAC	1 cut(s)	223
DdeI	CTNAG	2 cut(s)	84, 246
DpnI	GATC	1 cut(s)	149
DpnII	GATC	1 cut(s)	147
EaeI	YGGCCR	1 cut(s)	123
Eam1104I	CTCTTC	1 cut(s)	46
EarI	CTCTTC	1 cut(s)	46
Eco88I	CYCGRG	1 cut(s)	185
Eco91I	GGTNACC	1 cut(s)	70
EcoO65I	GGTNACC	1 cut(s)	70
EcoRII	CCWGG	1 cut(s)	359
EcoT22I	ATGCAT	1 cut(s)	6
Esp3I	CGTCTC	1 cut(s)	278
FaeI	CATG	2 cut(s)	8, 79
FaiI	YATR	5 cut(s)	6, 77, 312, 393, 408
FatI	CATG	2 cut(s)	4, 75
FauI	CCCGC	1 cut(s)	292
FblI	GTMKAC	1 cut(s)	441
Fnu4HI	GCNGC	5 cut(s)	126, 129, 201, 378, 381
FokI	GGATG	1 cut(s)	98
Fsp4HI	GCNGC	5 cut(s)	126, 129, 201, 378, 381
FspBI	CTAG	1 cut(s)	194
GlaI	GCGC	1 cut(s)	80
GluI	GCNGC	5 cut(s)	126, 129, 201, 378, 381
HaeIII	GGCC	2 cut(s)	125, 200
HapII	CCGG	1 cut(s)	277
HgaI	GACGC	1 cut(s)	295
HhaI	GCGC	1 cut(s)	81
Hin1I	GRCGYC	1 cut(s)	174
Hin1II	CATG	2 cut(s)	8, 79
Hin6I	GCGC	1 cut(s)	79
HinP1I	GCGC	1 cut(s)	79
HindIII	AAGCTT	1 cut(s)	314
HinfI	GANTC	1 cut(s)	338
HpaII	CCGG	1 cut(s)	277
HphI	GGTGA	2 cut(s)	64, 293
Hpy166II	GTNNAC	1 cut(s)	442
Hpy188I	TCNGA	2 cut(s)	220, 249
Hpy188III	TCNNGA	1 cut(s)	145
Hpy8I	GTNNAC	1 cut(s)	442
Hpy99I	CGWCG	1 cut(s)	346
HpyAV	CCTTC	2 cut(s)	107, 125
HpyCH4III	ACNGT	4 cut(s)	37, 70, 142, 271
HpyCH4IV	ACGT	1 cut(s)	174
HpyCH4V	TGCA	2 cut(s)	4, 32
HpyF10VI	GCNNNNNNNGC	2 cut(s)	87, 209
HpyF3I	CTNAG	2 cut(s)	84, 246
HpySE526I	ACGT	1 cut(s)	174
Hsp92I	GRCGYC	1 cut(s)	174
Hsp92II	CATG	2 cut(s)	8, 79
HspAI	GCGC	1 cut(s)	79
Kzo9I	GATC	1 cut(s)	147
LmnI	GCTCC	2 cut(s)	279, 421
LpnPI	CCDG	7 cut(s)	107, 148, 158, 290, 346, 373, 397
LweI	GCATC	1 cut(s)	433
MaeI	CTAG	1 cut(s)	194
MaeII	ACGT	1 cut(s)	174
MaeIII	GTNAC	2 cut(s)	17, 70
MalI	GATC	1 cut(s)	149
MboI	GATC	1 cut(s)	147
MboII	GAAGA	2 cut(s)	63, 391
MhlI	GDGCHC	2 cut(s)	276, 305
MluCI	AATT	2 cut(s)	388, 434
MlyI	GAGTC	1 cut(s)	347
MmeI	TCCRAC	2 cut(s)	137, 198
MnlI	CCTC	9 cut(s)	93, 101, 104, 119, 129, 163, 181, 190, 388
Mph1103I	ATGCAT	1 cut(s)	6
MseI	TTAA	1 cut(s)	60
MspI	CCGG	1 cut(s)	277
MspR9I	CCNGG	2 cut(s)	278, 361
MvaI	CCWGG	1 cut(s)	361
MwoI	GCNNNNNNNGC	2 cut(s)	87, 209
NciI	CCSGG	1 cut(s)	278
NdeII	GATC	1 cut(s)	147
NlaIII	CATG	2 cut(s)	8, 79
NlaIV	GGNNCC	1 cut(s)	373
NmuCI	GTSAC	2 cut(s)	17, 70
NsiI	ATGCAT	1 cut(s)	6
NspI	RCATGY	1 cut(s)	8
NspV	TTCGAA	1 cut(s)	455
PaeI	GCATGC	1 cut(s)	8
PkrI	GCNGC	5 cut(s)	127, 130, 202, 379, 382
PleI	GAGTC	1 cut(s)	346
PpsI	GAGTC	1 cut(s)	346
Psp6I	CCWGG	1 cut(s)	359
PspEI	GGTNACC	1 cut(s)	70
PspGI	CCWGG	1 cut(s)	359
PspN4I	GGNNCC	1 cut(s)	373
RsaI	GTAC	1 cut(s)	224
RsaNI	GTAC	1 cut(s)	223
SaqAI	TTAA	1 cut(s)	60
SatI	GCNGC	5 cut(s)	126, 129, 201, 378, 381
Sau3AI	GATC	1 cut(s)	147
SchI	GAGTC	1 cut(s)	347
ScrFI	CCNGG	2 cut(s)	278, 361
SduI	GDGCHC	2 cut(s)	276, 305
SetI	ASST	4 cut(s)	177, 192, 318, 399
SfaNI	GCATC	1 cut(s)	433
SfuI	TTCGAA	1 cut(s)	455
SphI	GCATGC	1 cut(s)	8
Sse9I	AATT	2 cut(s)	388, 434
SsiI	CCGC	8 cut(s)	126, 129, 153, 201, 299, 375, 378, 381
SspMI	CTAG	1 cut(s)	194
StyD4I	CCNGG	2 cut(s)	276, 359
TaaI	ACNGT	4 cut(s)	37, 70, 142, 271
TaiI	ACGT	1 cut(s)	177
TaqI	TCGA	2 cut(s)	49, 455
TasI	AATT	2 cut(s)	388, 434
TatI	WGTACW	1 cut(s)	222
TauI	GCSGC	5 cut(s)	128, 131, 203, 380, 383
Tru1I	TTAA	1 cut(s)	60
Tru9I	TTAA	1 cut(s)	60
TscAI	CASTG	2 cut(s)	92, 276
TseFI	GTSAC	2 cut(s)	17, 70
Tsp45I	GTSAC	2 cut(s)	17, 70
TspDTI	ATGAA	2 cut(s)	310, 437
TspRI	CASTG	2 cut(s)	92, 276
XceI	RCATGY	1 cut(s)	8
XmiI	GTMKAC	1 cut(s)	441
XspI	CTAG	1 cut(s)	194
ZraI	GACGTC	1 cut(s)	175
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.