FvH4_2g00771

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000842
Physical Location & Seq
Reverse (-)
789455 .. 798002
8548 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g00771.t1

Sequence Viewer

Length: 921 bp
ATGGACTATTCGAAGCTTGAAGAAGGCGATGCTTGTTCTTCTCAAATCAATGAATATGATCCCAATATCGACCTTGATGTTGCTCTTGCTTACATTGAGATTGGTGAACAAGTCTTTTTCCCTGCTCAAATTGTTTCTGGGGTGGCAAAACAACTCTCCCTTTATCAACAAAATAATCTTGATAGATCTCACCTTCATGCTCACCCTCCTTCTCCCCATATTAAATGGCATCCTCCCCCTCCAAATAGTCTTAAAGCCAATTTTGATGGTTCGGTGCTTCCTCTTTCCCGCGCTGCTGTTGGTTTTGTAGTTCATAATTTTGATGGCCTCCCAATTCTTGCTACAAGCCAAAATCTTGGTCGGATGGACATTCTTCTTGCAGAGGCTGTTGCTCTTCGTGCTGGCCTACAGTTGCTTGCTACCTACACTAATCAACCAATTATTGTGAAAGAAGACTCTAAGCTTCTCCGGGATGCGCTCAATGTTCGTTGTCAGATTCCATGGCGCCTTGAAGTGTTAGTTCAAGATATCCGAGCTTTATCATCCCATTTTCAGTCTATTTCCTTTAAACATGTTTGGAGAGAAGCAAATTTTTTAGCTGATAGTCTAGCTAAATTAGGACACTCTTGTACTAGTCCTCAGCTATGGATTTCTTCTCTTCCTCTTTCCTCGGCTCCCGCCTTCCAGCTTGATCTTTATGGGGGCGGCGCCCCCCCTATGGTGCCAACGTTAGGCCTTCAGCCTCATCGGTGTAAGTTGGGTGCGACCAGACGGGCTGCCTTGGCAATAGATCTGGCCTCGGGGAAGAGATGGATCCTTAAAGTTTTAAAGCGGTATCTTGCGCCAAGGTTGAGAATAGCGGTGTCTATGCGATTTCACATATCACCTCTGGATCAAGCCGATGGCTTTGCGGTGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

307

Amino Acids

33.81

Weight (kDa)

8.87

Isoelectric Point (pI)

54.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 87 - 206 2.9e-18 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000842)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15420 FvH4_2g00771 FvH4_3g29363
malus_domestica MD02G1293300.v1.1 MD06G1023000.v1.1 MD09G1232900.v1.1
prunus_persica Prupe.1G181500_v2.0.a1 Prupe.1G572100_v2.0.a1 Prupe.2G095500_v2.0.a1 Prupe.3G150400_v2.0.a1 Prupe.3G156200_v2.0.a1 Prupe.4G274400_v2.0.a1 Prupe.5G070700_v2.0.a1 Prupe.7G038000_v2.0.a1 Prupe.7G164000_v2.0.a1 Prupe.8G056500_v2.0.a1
pyrus_communis pycom04g02040 pycom05g04600 pycom07g05610 pycom08g16130 pycom11g16470 pycom12g07830 pycom15g38190 pycom215g00040
rosa_chinensis RchiOBHm_Chr1g0348881 RchiOBHm_Chr5g0032951 RchiOBHm_Chr6g0273271 RchiOBHm_Chr7g0218191 RchiOBHm_Chr7g0223721
rosa_multiflora Rmu_co8286615.1_g000001 Rmu_sc0001354.1_g000002 Rmu_sc0001925.1_g000003 Rmu_sc0001925.1_g000005 Rmu_sc0002221.1_g000004 Rmu_sc0002316.1_g000096 Rmu_sc0003291.1_g000005 Rmu_sc0003413.1_g000005 Rmu_sc0003542.1_g000011 Rmu_sc0004035.1_g000003 Rmu_sc0004035.1_g000004 Rmu_sc0004145.1_g000016 Rmu_sc0004660.1_g000004 Rmu_sc0005999.1_g000006 Rmu_sc0006123.1_g000002 Rmu_sc0006586.1_g000006 Rmu_sc0006889.1_g000029 Rmu_sc0008035.1_g000014 Rmu_sc0011962.1_g000002 Rmu_sc0029950.1_g000001 Rmu_sc0042409.1_g000001 Rmu_ssc0000238.1_g000013
rosa_roxburghii Rroxscaffold_2G00137410 Rroxscaffold_4G00312010 Rroxscaffold_4G00320800 Rroxscaffold_5G00341720 Rroxscaffold_5G00353530 Rroxscaffold_5G00360290 Rroxscaffold_7G00214170 Rroxscaffold_7G00215920
rosa_rugosa Rorug02G0157000
rosa_samantha Rh4BG196500 Rh6CG346400 Rh7BG241100 Rh7DG316300
rosa_wichuraiana Rw1G003610 Rw7G012170 Rw7G030150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 504, 707, 721
AccII CGCG 1 cut(s) 291
AciI CCGC 6 cut(s) 289, 678, 705, 832, 860, 911
AclI AACGTT 1 cut(s) 728
AclWI GGATC 4 cut(s) 53, 808, 821, 900
AcsI RAATTY 1 cut(s) 589
AcuI CTGAAG 1 cut(s) 722
AcyI GRCGYC 2 cut(s) 505, 708
AfaI GTAC 1 cut(s) 631
AfiI CCNNNNNNNGG 2 cut(s) 718, 731
AflIII ACRYGT 1 cut(s) 571
AgsI TTSAA 3 cut(s) 20, 512, 524
AhlI ACTAGT 1 cut(s) 632
AluBI AGCT 7 cut(s) 16, 463, 536, 599, 611, 643, 688
AluI AGCT 7 cut(s) 16, 463, 536, 599, 611, 643, 688
AlwI GGATC 4 cut(s) 53, 808, 821, 900
AlwNI CAGNNNCTG 1 cut(s) 386
Ama87I CYCGRG 1 cut(s) 799
AoxI GGCC 4 cut(s) 325, 403, 733, 795
ApeKI GCWGC 2 cut(s) 293, 776
ApoI RAATTY 1 cut(s) 589
ArsI GACNNNNNNTTYG 2 cut(s) 343, 375
AspLEI GCGC 5 cut(s) 293, 478, 507, 710, 844
AsuC2I CCSGG 1 cut(s) 470
AsuHPI GGTGA 4 cut(s) 116, 182, 194, 876
AsuII TTCGAA 1 cut(s) 11
AvaI CYCGRG 1 cut(s) 799
BamHI GGATCC 1 cut(s) 813
BanI GGYRCC 3 cut(s) 504, 707, 721
BbsI GAAGAC 1 cut(s) 459
BbvCI CCTCAGC 1 cut(s) 639
BbvI GCAGC 2 cut(s) 280, 763
BccI CCATC 5 cut(s) 260, 317, 358, 804, 896
BcgI CGANNNNNNTGC 1 cut(s) 890
BcnI CCSGG 1 cut(s) 470
BcuI ACTAGT 1 cut(s) 632
BfaI CTAG 2 cut(s) 608, 633
BfmI CTRYAG 1 cut(s) 407
BfoI RGCGCY 2 cut(s) 508, 711
BglII AGATCT 2 cut(s) 185, 790
BisI GCNGC 3 cut(s) 294, 706, 777
BlsI GCNGC 3 cut(s) 295, 707, 778
Bme1390I CCNGG 1 cut(s) 470
BmeT110I CYCGRG 1 cut(s) 799
BmiI GGNNCC 5 cut(s) 506, 675, 709, 723, 815
BmrFI CCNGG 1 cut(s) 470
BmsI GCATC 3 cut(s) 19, 238, 463
BpiI GAAGAC 1 cut(s) 459
Bpu10I CCTNAGC 1 cut(s) 639
Bpu14I TTCGAA 1 cut(s) 11
BpuMI CCSGG 1 cut(s) 470
BsaHI GRCGYC 2 cut(s) 505, 708
BsaJI CCNNGG 5 cut(s) 500, 669, 780, 798, 845
Bsc4I CCNNNNNNNGG 2 cut(s) 718, 731
BseDI CCNNGG 5 cut(s) 500, 669, 780, 798, 845
BseGI GGATG 4 cut(s) 229, 369, 478, 542
BseLI CCNNNNNNNGG 2 cut(s) 718, 731
BseMII CTCAG 1 cut(s) 653
BseXI GCAGC 2 cut(s) 280, 763
Bsh1236I CGCG 1 cut(s) 291
BshFI GGCC 4 cut(s) 327, 405, 735, 797
BshNI GGYRCC 3 cut(s) 504, 707, 721
BsiHKCI CYCGRG 1 cut(s) 799
BsiSI CCGG 1 cut(s) 469
BslI CCNNNNNNNGG 2 cut(s) 718, 731
BsnI GGCC 4 cut(s) 327, 405, 735, 797
BsoBI CYCGRG 1 cut(s) 799
Bsp119I TTCGAA 1 cut(s) 11
Bsp143I GATC 6 cut(s) 58, 185, 691, 790, 813, 892
Bsp19I CCATGG 1 cut(s) 500
BspACI CCGC 6 cut(s) 289, 678, 705, 832, 860, 911
BspANI GGCC 4 cut(s) 327, 405, 735, 797
BspCNI CTCAG 1 cut(s) 652
BspFNI CGCG 1 cut(s) 291
BspLI GGNNCC 5 cut(s) 506, 675, 709, 723, 815
BspPI GGATC 4 cut(s) 53, 808, 821, 900
BspQI GCTCTTC 1 cut(s) 399
BspT104I TTCGAA 1 cut(s) 11
BspT107I GGYRCC 3 cut(s) 504, 707, 721
BssECI CCNNGG 5 cut(s) 500, 669, 780, 798, 845
BssMI GATC 6 cut(s) 58, 185, 691, 790, 813, 892
BssNI GRCGYC 2 cut(s) 505, 708
BssT1I CCWWGG 3 cut(s) 500, 780, 845
Bst4CI ACNGT 1 cut(s) 411
Bst6I CTCTTC 3 cut(s) 399, 663, 800
BstACI GRCGYC 2 cut(s) 505, 708
BstBI TTCGAA 1 cut(s) 11
BstC8I GCNNGC 2 cut(s) 403, 417
BstDEI CTNAG 2 cut(s) 459, 639
BstDSI CCRYGG 1 cut(s) 500
BstF5I GGATG 4 cut(s) 229, 369, 478, 542
BstFNI CGCG 1 cut(s) 291
BstH2I RGCGCY 2 cut(s) 508, 711
BstHHI GCGC 5 cut(s) 293, 478, 507, 710, 844
BstKTI GATC 6 cut(s) 61, 188, 694, 793, 816, 895
BstMBI GATC 6 cut(s) 58, 185, 691, 790, 813, 892
BstMWI GCNNNNNNNGC 2 cut(s) 398, 782
BstNSI RCATGY 1 cut(s) 575
BstSCI CCNGG 1 cut(s) 468
BstSFI CTRYAG 1 cut(s) 407
BstUI CGCG 1 cut(s) 291
BstV1I GCAGC 2 cut(s) 280, 763
BstV2I GAAGAC 1 cut(s) 459
BstX2I RGATCY 3 cut(s) 185, 790, 813
BstXI CCANNNNNNTGG 1 cut(s) 356
BstYI RGATCY 3 cut(s) 185, 790, 813
BsuRI GGCC 4 cut(s) 327, 405, 735, 797
BtgI CCRYGG 1 cut(s) 500
BtgZI GCGATG 1 cut(s) 42
BtsCI GGATG 4 cut(s) 229, 369, 478, 542
Cac8I GCNNGC 2 cut(s) 403, 417
CaiI CAGNNNCTG 1 cut(s) 386
CfoI GCGC 5 cut(s) 293, 478, 507, 710, 844
Csp6I GTAC 1 cut(s) 630
CviAII CATG 3 cut(s) 197, 501, 572
CviQI GTAC 1 cut(s) 630
DdeI CTNAG 2 cut(s) 459, 639
DinI GGCGCC 2 cut(s) 506, 709
DpnI GATC 6 cut(s) 60, 187, 693, 792, 815, 894
DpnII GATC 6 cut(s) 58, 185, 691, 790, 813, 892
DraI TTTAAA 2 cut(s) 568, 828
Eam1104I CTCTTC 3 cut(s) 399, 663, 800
EarI CTCTTC 3 cut(s) 399, 663, 800
Eco130I CCWWGG 3 cut(s) 500, 780, 845
Eco147I AGGCCT 1 cut(s) 735
Eco32I GATATC 1 cut(s) 529
Eco57I CTGAAG 1 cut(s) 722
Eco88I CYCGRG 1 cut(s) 799
EcoRV GATATC 1 cut(s) 529
EcoT14I CCWWGG 3 cut(s) 500, 780, 845
EgeI GGCGCC 2 cut(s) 506, 709
EheI GGCGCC 2 cut(s) 506, 709
ErhI CCWWGG 3 cut(s) 500, 780, 845
FaeI CATG 3 cut(s) 200, 504, 575
FatI CATG 3 cut(s) 196, 500, 571
FauI CCCGC 2 cut(s) 296, 685
Fnu4HI GCNGC 3 cut(s) 294, 706, 777
FokI GGATG 4 cut(s) 216, 376, 485, 529
Fsp4HI GCNGC 3 cut(s) 294, 706, 777
FspBI CTAG 2 cut(s) 608, 633
GlaI GCGC 5 cut(s) 292, 477, 506, 709, 843
GluI GCNGC 3 cut(s) 294, 706, 777
HaeII RGCGCY 2 cut(s) 508, 711
HaeIII GGCC 4 cut(s) 327, 405, 735, 797
HapII CCGG 1 cut(s) 469
HhaI GCGC 5 cut(s) 293, 478, 507, 710, 844
Hin1I GRCGYC 2 cut(s) 505, 708
Hin1II CATG 3 cut(s) 200, 504, 575
Hin6I GCGC 5 cut(s) 291, 476, 505, 708, 842
HinP1I GCGC 5 cut(s) 291, 476, 505, 708, 842
HindIII AAGCTT 2 cut(s) 14, 461
HinfI GANTC 2 cut(s) 455, 496
HpaII CCGG 1 cut(s) 469
HphI GGTGA 4 cut(s) 116, 182, 194, 876
Hpy166II GTNNAC 1 cut(s) 107
Hpy188I TCNGA 3 cut(s) 363, 495, 533
Hpy188III TCNNGA 3 cut(s) 179, 524, 890
Hpy8I GTNNAC 1 cut(s) 107
HpyAV CCTTC 5 cut(s) 17, 203, 219, 691, 746
HpyCH4III ACNGT 1 cut(s) 411
HpyCH4IV ACGT 1 cut(s) 728
HpyCH4V TGCA 1 cut(s) 380
HpyF10VI GCNNNNNNNGC 2 cut(s) 398, 782
HpyF3I CTNAG 2 cut(s) 459, 639
HpySE526I ACGT 1 cut(s) 728
Hsp92I GRCGYC 2 cut(s) 505, 708
Hsp92II CATG 3 cut(s) 200, 504, 575
HspAI GCGC 5 cut(s) 291, 476, 505, 708, 842
KasI GGCGCC 2 cut(s) 504, 707
Kzo9I GATC 6 cut(s) 58, 185, 691, 790, 813, 892
LguI GCTCTTC 1 cut(s) 399
LmnI GCTCC 1 cut(s) 679
LpnPI CCDG 8 cut(s) 123, 135, 387, 482, 698, 779, 781, 875
Lsp1109I GCAGC 2 cut(s) 280, 763
LweI GCATC 3 cut(s) 19, 238, 463
MaeI CTAG 2 cut(s) 608, 633
MaeII ACGT 1 cut(s) 728
MalI GATC 6 cut(s) 60, 187, 693, 792, 815, 894
MboI GATC 6 cut(s) 58, 185, 691, 790, 813, 892
MboII GAAGA 8 cut(s) 30, 32, 365, 386, 464, 645, 650, 817
MflI RGATCY 3 cut(s) 185, 790, 813
MluCI AATT 7 cut(s) 129, 259, 316, 333, 438, 589, 614
Mly113I GGCGCC 2 cut(s) 505, 708
MlyI GAGTC 1 cut(s) 449
MmeI TCCRAC 1 cut(s) 341
MseI TTAA 5 cut(s) 222, 252, 567, 819, 827
MslI CAYNNNNRTG 1 cut(s) 195
MspI CCGG 1 cut(s) 469
MspR9I CCNGG 1 cut(s) 470
MvnI CGCG 1 cut(s) 291
MwoI GCNNNNNNNGC 2 cut(s) 398, 782
NarI GGCGCC 2 cut(s) 505, 708
NciI CCSGG 1 cut(s) 470
NcoI CCATGG 1 cut(s) 500
NdeII GATC 6 cut(s) 58, 185, 691, 790, 813, 892
NlaIII CATG 3 cut(s) 200, 504, 575
NlaIV GGNNCC 5 cut(s) 506, 675, 709, 723, 815
NmeAIII GCCGAG 1 cut(s) 650
NspI RCATGY 1 cut(s) 575
NspV TTCGAA 1 cut(s) 11
PceI AGGCCT 1 cut(s) 735
PciI ACATGT 1 cut(s) 571
PciSI GCTCTTC 1 cut(s) 399
PfeI GAWTC 1 cut(s) 496
PfoI TCCNGGA 1 cut(s) 468
PkrI GCNGC 3 cut(s) 295, 707, 778
PleI GAGTC 1 cut(s) 449
PluTI GGCGCC 2 cut(s) 508, 711
PpsI GAGTC 1 cut(s) 449
PscI ACATGT 1 cut(s) 571
Psp1406I AACGTT 1 cut(s) 728
PspN4I GGNNCC 5 cut(s) 506, 675, 709, 723, 815
PstNI CAGNNNCTG 1 cut(s) 386
PsuI RGATCY 3 cut(s) 185, 790, 813
RsaI GTAC 1 cut(s) 631
RsaNI GTAC 1 cut(s) 630
RseI CAYNNNNRTG 1 cut(s) 195
SapI GCTCTTC 1 cut(s) 399
SaqAI TTAA 5 cut(s) 222, 252, 567, 819, 827
SatI GCNGC 3 cut(s) 294, 706, 777
Sau3AI GATC 6 cut(s) 58, 185, 691, 790, 813, 892
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 1 cut(s) 470
SfaNI GCATC 3 cut(s) 19, 238, 463
SfcI CTRYAG 1 cut(s) 407
SfoI GGCGCC 2 cut(s) 506, 709
SfuI TTCGAA 1 cut(s) 11
SmiMI CAYNNNNRTG 1 cut(s) 195
SpeI ACTAGT 1 cut(s) 632
Sse9I AATT 7 cut(s) 129, 259, 316, 333, 438, 589, 614
SseBI AGGCCT 1 cut(s) 735
SsiI CCGC 6 cut(s) 289, 678, 705, 832, 860, 911
SspDI GGCGCC 2 cut(s) 504, 707
SspMI CTAG 2 cut(s) 608, 633
StuI AGGCCT 1 cut(s) 735
StyD4I CCNGG 1 cut(s) 468
StyI CCWWGG 3 cut(s) 500, 780, 845
TaaI ACNGT 1 cut(s) 411
TaiI ACGT 1 cut(s) 731
TaqI TCGA 2 cut(s) 11, 69
TasI AATT 7 cut(s) 129, 259, 316, 333, 438, 589, 614
TatI WGTACW 1 cut(s) 629
TauI GCSGC 1 cut(s) 708
TfiI GAWTC 1 cut(s) 496
Tru1I TTAA 5 cut(s) 222, 252, 567, 819, 827
Tru9I TTAA 5 cut(s) 222, 252, 567, 819, 827
TseI GCWGC 2 cut(s) 293, 776
TspDTI ATGAA 3 cut(s) 66, 185, 302
XapI RAATTY 1 cut(s) 589
XceI RCATGY 1 cut(s) 575
XspI CTAG 2 cut(s) 608, 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.