pycom05g04600

beta-mannosidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000842
Physical Location & Seq
Forward (+)
5426325 .. 5427783
1459 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g04600.2

Sequence Viewer

Length: 615 bp
ATGGGCGTGTGTGCTCGTATCCCCCTTCCCGGATTTGATCTGCATACAGTGAAGTACTTCCTTTTCTCTGGAGCATTTAGCAGGAGCATCTGGTACCAAATCCTTTCCATCTCAAGTTTTGTGCACAACCAGAACATCTCAAGTTTTGTGGATCTTTCTAGCAATTCTGTGAGCTGGAATTTTGATTTTAGGAGAAATCTGAATGAGGTGGAGATAGAAGAGGTGGCTAGATTATTACAAAATGTGGAAAATGTTCGCTTGTCTCAATCAAGAGCGGATAACAGAAGGTGGAAACTGGACTTATCAGGATTGTTCACTTGCAAATCCTATCGCTCTTTCTTGAGCAACAATGGGATTGTGCAACATTTTCCACCCTCTTCTCAGATTTGGAAATCAAAAGTTCCTCCTAAGGTCAAAATTCTTGTGTGGCTAGTGGCTAATGGGAAACTCAACACTTGTGATCAAATTCAAAGGAGAAGCCCTTTTATTTGTTTCTCTCCCCATTGGTGCAGTTTGTGTAAAGCTAAGGAGGAGAGTGTTAACCACATTTTTCTCCATTGTTCTTACACGATTCAACTGTGGTGGAAATTGTTTCAGGAGGTTAGAGTTAGTTAG

Protein Analysis

205

Amino Acids

23.81

Weight (kDa)

9.5

Isoelectric Point (pI)

59.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000842)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15420 FvH4_2g00771 FvH4_3g29363
malus_domestica MD02G1293300.v1.1 MD06G1023000.v1.1 MD09G1232900.v1.1
prunus_persica Prupe.1G181500_v2.0.a1 Prupe.1G572100_v2.0.a1 Prupe.2G095500_v2.0.a1 Prupe.3G150400_v2.0.a1 Prupe.3G156200_v2.0.a1 Prupe.4G274400_v2.0.a1 Prupe.5G070700_v2.0.a1 Prupe.7G038000_v2.0.a1 Prupe.7G164000_v2.0.a1 Prupe.8G056500_v2.0.a1
pyrus_communis pycom04g02040 pycom05g04600 pycom07g05610 pycom08g16130 pycom11g16470 pycom12g07830 pycom15g38190 pycom215g00040
rosa_chinensis RchiOBHm_Chr1g0348881 RchiOBHm_Chr5g0032951 RchiOBHm_Chr6g0273271 RchiOBHm_Chr7g0218191 RchiOBHm_Chr7g0223721
rosa_multiflora Rmu_co8286615.1_g000001 Rmu_sc0001354.1_g000002 Rmu_sc0001925.1_g000003 Rmu_sc0001925.1_g000005 Rmu_sc0002221.1_g000004 Rmu_sc0002316.1_g000096 Rmu_sc0003291.1_g000005 Rmu_sc0003413.1_g000005 Rmu_sc0003542.1_g000011 Rmu_sc0004035.1_g000003 Rmu_sc0004035.1_g000004 Rmu_sc0004145.1_g000016 Rmu_sc0004660.1_g000004 Rmu_sc0005999.1_g000006 Rmu_sc0006123.1_g000002 Rmu_sc0006586.1_g000006 Rmu_sc0006889.1_g000029 Rmu_sc0008035.1_g000014 Rmu_sc0011962.1_g000002 Rmu_sc0029950.1_g000001 Rmu_sc0042409.1_g000001 Rmu_ssc0000238.1_g000013
rosa_roxburghii Rroxscaffold_2G00137410 Rroxscaffold_4G00312010 Rroxscaffold_4G00320800 Rroxscaffold_5G00341720 Rroxscaffold_5G00353530 Rroxscaffold_5G00360290 Rroxscaffold_7G00214170 Rroxscaffold_7G00215920
rosa_rugosa Rorug02G0157000
rosa_samantha Rh4BG196500 Rh6CG346400 Rh7BG241100 Rh7DG316300
rosa_wichuraiana Rw1G003610 Rw7G012170 Rw7G030150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 93
AccB1I GGYRCC 1 cut(s) 93
AccBSI CCGCTC 1 cut(s) 275
AciI CCGC 1 cut(s) 275
AclWI GGATC 1 cut(s) 159
AcsI RAATTY 3 cut(s) 178, 417, 465
AfaI GTAC 2 cut(s) 56, 95
AfiI CCNNNNNNNGG 1 cut(s) 29
AgsI TTSAA 2 cut(s) 470, 575
AluBI AGCT 2 cut(s) 174, 524
AluI AGCT 2 cut(s) 174, 524
Alw21I GWGCWC 2 cut(s) 16, 126
Alw26I GTCTC 1 cut(s) 267
Alw44I GTGCAC 1 cut(s) 122
AlwI GGATC 1 cut(s) 159
ApaLI GTGCAC 1 cut(s) 122
ApoI RAATTY 3 cut(s) 178, 417, 465
Asp700I GAANNNNTTC 2 cut(s) 56, 252
Asp718I GGTACC 1 cut(s) 93
AsuC2I CCSGG 1 cut(s) 30
AxyI CCTNAGG 1 cut(s) 408
BaeGI GKGCMC 1 cut(s) 126
BanI GGYRCC 1 cut(s) 93
Bbv12I GWGCWC 2 cut(s) 16, 126
BccI CCATC 1 cut(s) 116
BciVI GTATCC 1 cut(s) 29
BclI TGATCA 1 cut(s) 460
BcnI CCSGG 1 cut(s) 30
BcoDI GTCTC 1 cut(s) 267
BfaI CTAG 3 cut(s) 159, 228, 431
BfuI GTATCC 1 cut(s) 29
BmcAI AGTACT 1 cut(s) 56
Bme1390I CCNGG 1 cut(s) 30
BmiI GGNNCC 1 cut(s) 95
BmrFI CCNGG 1 cut(s) 30
BmsI GCATC 1 cut(s) 96
BpmI CTGGAG 1 cut(s) 90
Bpu10I CCTNAGC 1 cut(s) 525
BpuEI CTTGAG 3 cut(s) 97, 124, 361
BpuMI CCSGG 1 cut(s) 30
Bsc4I CCNNNNNNNGG 1 cut(s) 29
Bse1I ACTGG 1 cut(s) 300
Bse21I CCTNAGG 1 cut(s) 408
BseLI CCNNNNNNNGG 1 cut(s) 29
BseMII CTCAG 1 cut(s) 395
BseNI ACTGG 1 cut(s) 300
BseRI GAGGAG 1 cut(s) 545
BseSI GKGCMC 1 cut(s) 126
BsgI GTGCAG 1 cut(s) 529
BshNI GGYRCC 1 cut(s) 93
BsiHKAI GWGCWC 2 cut(s) 16, 126
BsiSI CCGG 1 cut(s) 30
BslI CCNNNNNNNGG 1 cut(s) 29
BsmAI GTCTC 1 cut(s) 267
Bsp1286I GDGCHC 2 cut(s) 16, 126
Bsp143I GATC 3 cut(s) 37, 151, 460
BspACI CCGC 1 cut(s) 275
BspCNI CTCAG 1 cut(s) 394
BspLI GGNNCC 1 cut(s) 95
BspPI GGATC 1 cut(s) 159
BspT107I GGYRCC 1 cut(s) 93
BsrBI CCGCTC 1 cut(s) 275
BsrI ACTGG 1 cut(s) 300
BssMI GATC 3 cut(s) 37, 151, 460
Bst4CI ACNGT 2 cut(s) 49, 579
Bst6I CTCTTC 2 cut(s) 213, 382
BstDEI CTNAG 3 cut(s) 381, 408, 525
BstKTI GATC 3 cut(s) 40, 154, 463
BstMAI GTCTC 1 cut(s) 267
BstMBI GATC 3 cut(s) 37, 151, 460
BstSCI CCNGG 1 cut(s) 28
BstSLI GKGCMC 1 cut(s) 126
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
Bsu36I CCTNAGG 1 cut(s) 408
BsuI GTATCC 1 cut(s) 29
BtsIMutI CAGTG 1 cut(s) 54
Csp6I GTAC 2 cut(s) 55, 94
CspCI CAANNNNNGTGG 4 cut(s) 129, 164, 563, 598
CviJI RGCY 6 cut(s) 174, 227, 430, 437, 480, 524
CviKI_1 RGCY 6 cut(s) 174, 227, 430, 437, 480, 524
CviQI GTAC 2 cut(s) 55, 94
DdeI CTNAG 3 cut(s) 381, 408, 525
DpnI GATC 3 cut(s) 39, 153, 462
DpnII GATC 3 cut(s) 37, 151, 460
Eam1104I CTCTTC 2 cut(s) 213, 382
EarI CTCTTC 2 cut(s) 213, 382
Eco81I CCTNAGG 1 cut(s) 408
FaiI YATR 1 cut(s) 45
FbaI TGATCA 1 cut(s) 460
FspBI CTAG 3 cut(s) 159, 228, 431
GsuI CTGGAG 1 cut(s) 90
HapII CCGG 1 cut(s) 30
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HinfI GANTC 1 cut(s) 571
HpaI GTTAAC 1 cut(s) 541
HpaII CCGG 1 cut(s) 30
Hpy166II GTNNAC 3 cut(s) 124, 315, 541
Hpy188I TCNGA 2 cut(s) 201, 384
Hpy188III TCNNGA 5 cut(s) 69, 270, 306, 340, 596
Hpy8I GTNNAC 3 cut(s) 124, 315, 541
HpyAV CCTTC 2 cut(s) 35, 279
HpyCH4III ACNGT 2 cut(s) 49, 579
HpyCH4V TGCA 5 cut(s) 43, 124, 321, 361, 510
HpyF3I CTNAG 3 cut(s) 381, 408, 525
KpnI GGTACC 1 cut(s) 97
Ksp22I TGATCA 1 cut(s) 460
KspAI GTTAAC 1 cut(s) 541
Kzo9I GATC 3 cut(s) 37, 151, 460
LmnI GCTCC 2 cut(s) 71, 84
LpnPI CCDG 9 cut(s) 43, 54, 67, 76, 143, 160, 281, 291, 581
LweI GCATC 1 cut(s) 96
MaeI CTAG 3 cut(s) 159, 228, 431
MalI GATC 3 cut(s) 39, 153, 462
MbiI CCGCTC 1 cut(s) 275
MboI GATC 3 cut(s) 37, 151, 460
MboII GAAGA 2 cut(s) 230, 369
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 2 cut(s) 16, 126
MluCI AATT 5 cut(s) 163, 178, 417, 465, 587
MnlI CCTC 6 cut(s) 199, 214, 385, 414, 523, 592
MroXI GAANNNNTTC 2 cut(s) 56, 252
MseI TTAA 1 cut(s) 540
MspI CCGG 1 cut(s) 30
MspR9I CCNGG 1 cut(s) 30
NciI CCSGG 1 cut(s) 30
NdeII GATC 3 cut(s) 37, 151, 460
NlaIV GGNNCC 1 cut(s) 95
PdmI GAANNNNTTC 2 cut(s) 56, 252
PfeI GAWTC 1 cut(s) 571
PfoI TCCNGGA 1 cut(s) 28
PspN4I GGNNCC 1 cut(s) 95
PsuI RGATCY 1 cut(s) 151
RsaI GTAC 2 cut(s) 56, 95
RsaNI GTAC 2 cut(s) 55, 94
SaqAI TTAA 1 cut(s) 540
Sau3AI GATC 3 cut(s) 37, 151, 460
ScaI AGTACT 1 cut(s) 56
ScrFI CCNGG 1 cut(s) 30
SduI GDGCHC 2 cut(s) 16, 126
SetI ASST 7 cut(s) 176, 210, 225, 290, 414, 526, 603
SfaNI GCATC 1 cut(s) 96
SmlI CTYRAG 3 cut(s) 112, 139, 340
SmoI CTYRAG 3 cut(s) 112, 139, 340
Sse9I AATT 5 cut(s) 163, 178, 417, 465, 587
SsiI CCGC 1 cut(s) 275
SspMI CTAG 3 cut(s) 159, 228, 431
StyD4I CCNGG 1 cut(s) 28
TaaI ACNGT 2 cut(s) 49, 579
TasI AATT 5 cut(s) 163, 178, 417, 465, 587
TatI WGTACW 1 cut(s) 54
TfiI GAWTC 1 cut(s) 571
Tru1I TTAA 1 cut(s) 540
Tru9I TTAA 1 cut(s) 540
TscAI CASTG 1 cut(s) 54
TspRI CASTG 1 cut(s) 54
VneI GTGCAC 1 cut(s) 122
XapI RAATTY 3 cut(s) 178, 417, 465
XmnI GAANNNNTTC 2 cut(s) 56, 252
XspI CTAG 3 cut(s) 159, 228, 431
ZrmI AGTACT 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.