Rmu_sc0004035.1_g000003

ribonuclease H protein At1g65750

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004035.1
OG0000842
Physical Location & Seq
Reverse (-)
18819 .. 19277
459 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004035.1_g000003.1.cds

Sequence Viewer

Length: 459 bp
atgagctgggactttgggttcagaagaaacttaaatgatcaagagattgaggagtttgcctcgctgatggttaagctacaaaatgtccgattagtggattccaaaccagatgaaaggaggtggaagcttgagcctaatgggaaattctcttgcaaatctttccatagcttcttaattagtggtggatcagatccaatttttgctcctgcaaagtatatttggaaggtcaaggtccctactaaggtgaagattttgggatggcttgtggtactggggaagacgaatacatgtgatgttcttcaaaggagaagaccaggaagttgtttttcacctcactggtgcatttatgcaaagctcaaggggaaagtgctgatcatgtcttcgtgcattgtgaagtggctaatttcttatggaaaaaattatttagggaggcaagagtggactggacaactccattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

152

Amino Acids

17.65

Weight (kDa)

9.79

Isoelectric Point (pI)

57.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000842)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15420 FvH4_2g00771 FvH4_3g29363
malus_domestica MD02G1293300.v1.1 MD06G1023000.v1.1 MD09G1232900.v1.1
prunus_persica Prupe.1G181500_v2.0.a1 Prupe.1G572100_v2.0.a1 Prupe.2G095500_v2.0.a1 Prupe.3G150400_v2.0.a1 Prupe.3G156200_v2.0.a1 Prupe.4G274400_v2.0.a1 Prupe.5G070700_v2.0.a1 Prupe.7G038000_v2.0.a1 Prupe.7G164000_v2.0.a1 Prupe.8G056500_v2.0.a1
pyrus_communis pycom04g02040 pycom05g04600 pycom07g05610 pycom08g16130 pycom11g16470 pycom12g07830 pycom15g38190 pycom215g00040
rosa_chinensis RchiOBHm_Chr1g0348881 RchiOBHm_Chr5g0032951 RchiOBHm_Chr6g0273271 RchiOBHm_Chr7g0218191 RchiOBHm_Chr7g0223721
rosa_multiflora Rmu_co8286615.1_g000001 Rmu_sc0001354.1_g000002 Rmu_sc0001925.1_g000003 Rmu_sc0001925.1_g000005 Rmu_sc0002221.1_g000004 Rmu_sc0002316.1_g000096 Rmu_sc0003291.1_g000005 Rmu_sc0003413.1_g000005 Rmu_sc0003542.1_g000011 Rmu_sc0004035.1_g000003 Rmu_sc0004035.1_g000004 Rmu_sc0004145.1_g000016 Rmu_sc0004660.1_g000004 Rmu_sc0005999.1_g000006 Rmu_sc0006123.1_g000002 Rmu_sc0006586.1_g000006 Rmu_sc0006889.1_g000029 Rmu_sc0008035.1_g000014 Rmu_sc0011962.1_g000002 Rmu_sc0029950.1_g000001 Rmu_sc0042409.1_g000001 Rmu_ssc0000238.1_g000013
rosa_roxburghii Rroxscaffold_2G00137410 Rroxscaffold_4G00312010 Rroxscaffold_4G00320800 Rroxscaffold_5G00341720 Rroxscaffold_5G00353530 Rroxscaffold_5G00360290 Rroxscaffold_7G00214170 Rroxscaffold_7G00215920
rosa_rugosa Rorug02G0157000
rosa_samantha Rh4BG196500 Rh6CG346400 Rh7BG241100 Rh7DG316300
rosa_wichuraiana Rw1G003610 Rw7G012170 Rw7G030150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 185, 193
AcsI RAATTY 1 cut(s) 143
AfaI GTAC 1 cut(s) 270
AfiI CCNNNNNNNGG 2 cut(s) 94, 241
AflIII ACRYGT 1 cut(s) 287
AgsI TTSAA 1 cut(s) 302
AjnI CCWGG 1 cut(s) 313
AloI GAACNNNNNNTCC 1 cut(s) 34
AluBI AGCT 5 cut(s) 6, 76, 127, 168, 355
AluI AGCT 5 cut(s) 6, 76, 127, 168, 355
AlwI GGATC 2 cut(s) 185, 193
ApoI RAATTY 1 cut(s) 143
AspS9I GGNCC 1 cut(s) 232
AsuHPI GGTGA 2 cut(s) 256, 321
AvaII GGWCC 1 cut(s) 232
BbsI GAAGAC 3 cut(s) 284, 316, 372
BccI CCATC 2 cut(s) 61, 252
BciT130I CCWGG 1 cut(s) 315
BclI TGATCA 2 cut(s) 37, 372
Bme1390I CCNGG 1 cut(s) 315
Bme18I GGWCC 1 cut(s) 232
BmgT120I GGNCC 1 cut(s) 232
BmiI GGNNCC 1 cut(s) 234
BmrFI CCNGG 1 cut(s) 315
BmrI ACTGGG 1 cut(s) 281
BmuI ACTGGG 1 cut(s) 281
BpiI GAAGAC 3 cut(s) 284, 316, 372
BplI GAGNNNNNCTC 2 cut(s) 44, 76
BpuEI CTTGAG 2 cut(s) 149, 341
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 241
Bse1I ACTGG 3 cut(s) 276, 341, 448
BseBI CCWGG 1 cut(s) 315
BseGI GGATG 1 cut(s) 263
BseLI CCNNNNNNNGG 2 cut(s) 94, 241
BseNI ACTGG 3 cut(s) 276, 341, 448
BseRI GAGGAG 1 cut(s) 65
BseYI CCCAGC 1 cut(s) 6
BslFI GGGAC 2 cut(s) 23, 218
BslI CCNNNNNNNGG 2 cut(s) 94, 241
BsmFI GGGAC 2 cut(s) 23, 218
Bsp143I GATC 4 cut(s) 37, 185, 190, 372
BspLI GGNNCC 1 cut(s) 234
BspPI GGATC 2 cut(s) 185, 193
BsrI ACTGG 3 cut(s) 276, 341, 448
BssMI GATC 4 cut(s) 37, 185, 190, 372
Bst2UI CCWGG 1 cut(s) 315
BstDEI CTNAG 1 cut(s) 240
BstF5I GGATG 1 cut(s) 263
BstKTI GATC 4 cut(s) 40, 188, 193, 375
BstMBI GATC 4 cut(s) 37, 185, 190, 372
BstNI CCWGG 1 cut(s) 315
BstNSI RCATGY 1 cut(s) 291
BstSCI CCNGG 1 cut(s) 313
BstV2I GAAGAC 3 cut(s) 284, 316, 372
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
BtsCI GGATG 1 cut(s) 263
BtsIMutI CAGTG 1 cut(s) 334
Cfr13I GGNCC 1 cut(s) 232
Csp6I GTAC 1 cut(s) 269
CviAII CATG 2 cut(s) 288, 376
CviJI RGCY 8 cut(s) 6, 76, 127, 133, 168, 262, 355, 400
CviKI_1 RGCY 8 cut(s) 6, 76, 127, 133, 168, 262, 355, 400
CviQI GTAC 1 cut(s) 269
DdeI CTNAG 1 cut(s) 240
DpnI GATC 4 cut(s) 39, 187, 192, 374
DpnII GATC 4 cut(s) 37, 185, 190, 372
Eco47I GGWCC 1 cut(s) 232
EcoO109I RGGNCCY 1 cut(s) 232
EcoRII CCWGG 1 cut(s) 313
FaeI CATG 2 cut(s) 291, 379
FaiI YATR 6 cut(s) 165, 216, 289, 348, 377, 411
FaqI GGGAC 2 cut(s) 23, 218
FatI CATG 2 cut(s) 287, 375
FbaI TGATCA 2 cut(s) 37, 372
FokI GGATG 1 cut(s) 270
GsaI CCCAGC 1 cut(s) 10
Hin1II CATG 2 cut(s) 291, 379
HindIII AAGCTT 1 cut(s) 125
HinfI GANTC 1 cut(s) 98
HphI GGTGA 2 cut(s) 256, 321
Hpy166II GTNNAC 1 cut(s) 441
Hpy188I TCNGA 3 cut(s) 23, 89, 190
Hpy188III TCNNGA 1 cut(s) 41
Hpy8I GTNNAC 1 cut(s) 441
HpyAV CCTTC 1 cut(s) 217
HpyCH4V TGCA 5 cut(s) 153, 209, 342, 350, 387
HpyF3I CTNAG 1 cut(s) 240
Hsp92II CATG 2 cut(s) 291, 379
Ksp22I TGATCA 2 cut(s) 37, 372
Kzo9I GATC 4 cut(s) 37, 185, 190, 372
LmnI GCTCC 1 cut(s) 208
LpnPI CCDG 7 cut(s) 120, 219, 257, 300, 322, 327, 429
MalI GATC 4 cut(s) 39, 187, 192, 374
MboI GATC 4 cut(s) 37, 185, 190, 372
MboII GAAGA 6 cut(s) 36, 259, 289, 290, 321, 372
MflI RGATCY 1 cut(s) 190
MluCI AATT 5 cut(s) 143, 174, 195, 402, 418
MnlI CCTC 5 cut(s) 43, 70, 111, 342, 423
MseI TTAA 3 cut(s) 32, 72, 173
MspR9I CCNGG 1 cut(s) 315
MvaI CCWGG 1 cut(s) 315
NdeII GATC 4 cut(s) 37, 185, 190, 372
NlaIII CATG 2 cut(s) 291, 379
NlaIV GGNNCC 1 cut(s) 234
NspI RCATGY 1 cut(s) 291
PciI ACATGT 1 cut(s) 287
PfeI GAWTC 1 cut(s) 98
PpuMI RGGWCCY 1 cut(s) 232
PscI ACATGT 1 cut(s) 287
Psp5II RGGWCCY 1 cut(s) 232
Psp6I CCWGG 1 cut(s) 313
PspFI CCCAGC 1 cut(s) 6
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 1 cut(s) 234
PspPI GGNCC 1 cut(s) 232
PspPPI RGGWCCY 1 cut(s) 232
PsuI RGATCY 1 cut(s) 190
RsaI GTAC 1 cut(s) 270
RsaNI GTAC 1 cut(s) 269
SaqAI TTAA 3 cut(s) 32, 72, 173
Sau3AI GATC 4 cut(s) 37, 185, 190, 372
Sau96I GGNCC 1 cut(s) 232
ScrFI CCNGG 1 cut(s) 315
SinI GGWCC 1 cut(s) 232
SmlI CTYRAG 2 cut(s) 128, 356
SmoI CTYRAG 2 cut(s) 128, 356
Sse9I AATT 5 cut(s) 143, 174, 195, 402, 418
StyD4I CCNGG 1 cut(s) 313
TasI AATT 5 cut(s) 143, 174, 195, 402, 418
TfiI GAWTC 1 cut(s) 98
Tru1I TTAA 3 cut(s) 32, 72, 173
Tru9I TTAA 3 cut(s) 32, 72, 173
TscAI CASTG 1 cut(s) 341
TspDTI ATGAA 1 cut(s) 126
TspRI CASTG 1 cut(s) 341
VpaK11BI GGWCC 1 cut(s) 232
XapI RAATTY 1 cut(s) 143
XceI RCATGY 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.