Prupe.8G056500_v2.0.a1

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000842
Physical Location & Seq
Reverse (-)
6595580 .. 6597495
1916 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G056500.1

Sequence Viewer

Length: 1314 bp
ATGGATGATTTTAAGGAAGCCATTTCTGATTGCTGCTTCAAAGAAATACCTTTCTTGGGCTCTCCTTTTACTTGGAGCAGAGGGTCTGGTGGAAGCCTGGTCAAAGAGCATCTAGATAGAGATGCTTCAGACCATGCCCCCTTGGTTTTACATGCTGAAATTCATGCTCCTGCCACAACTTTTCGTAAATGGCGTTTTCATTTTGAAGCAATGTGGGCGAATGAAGAGGGCTACCGTAATACTATTCTTGAGGCTTGGGGTGAGGCCTCTTGTCAGTCTCTACCTCTCACAAACAAGTTTTTACTGTGTACACAACGTCTTAATTCTTGGGGCCAACAACAGTTTGGTTCTCTCCCAAAACAGATTCAGCAATGTTATTTGGAGCTCCAAAATCTTGACTTGGATGCACCCAATTTTGACCCAACCAGGAAGCATGAATTATCAGTTAAACTTGATGATCTACTTGAGAAAGAGGAGATTTTTTGGAAACAAAGGTCCCGAGTTCAGTGGCTTCAGGAGGGCGACTGGAAAACACATGGATTGCCTGCTTTATTTTTCCAAAAATACTGGGATATTATTGGAGAAGATATTGCTCTTATTTGCTTGCAGATCTTGAATAATGGCAAGAGTATTAAGGAGTTTAACCATACATTGGTGGCATTGATTCCGAAGATAAAGAACGTAAAGAATGTGAAGGAGTTCCGACCTATAAGTCTATGTAACGTCATCTACAAAATTGTTGCCAAAACTCTAGCTAACTGTCTGAAGATCATTTTGCCTGAGGTGATTTCAAGTGTTCAAAGTGCTTCTATTCCTGGAAAATATATCATTGATAATGTCACTCTTGCTTTTGAGCTTATGCATACAGTCAAAAAACAAAAACAAATTAAGGAGCCAAAAGTTGTTGTGAAGCTTGATGTCAGTAAGGCATATGATCGAGTTGAGCGGAATTTTCTTCGCAAAATCATGAAAAAGCTTGGCTTTGCAACTCGTTGGGTGGATTTGATTATGGAGTGTGTGACGACTGTTACTTATTCCATTATTAATTTGCTTACAACCTATGAAAAGGCAGCTGGGCAACGAATCAATTTTGAAAACTCTACTATGGCATTTGGTCCTAATTCTAATATTCAACTCCAAAGTGATATGCAGCTGATCCTTGGCATTCCATTAGTCCAATTCCATGAGAAATACCTTGGATTACCAATGGTTGTTGGACAAGCCAAGAAGGCTCTCTTTGTCAACCTACGCAACAGAGTTGGAAAACTTCTTCAGGGATGGAAAGGTCACGCGTTTGTTCACTCGAGTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

438

Amino Acids

50.12

Weight (kDa)

9.02

Isoelectric Point (pI)

33.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000842)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15420 FvH4_2g00771 FvH4_3g29363
malus_domestica MD02G1293300.v1.1 MD06G1023000.v1.1 MD09G1232900.v1.1
prunus_persica Prupe.1G181500_v2.0.a1 Prupe.1G572100_v2.0.a1 Prupe.2G095500_v2.0.a1 Prupe.3G150400_v2.0.a1 Prupe.3G156200_v2.0.a1 Prupe.4G274400_v2.0.a1 Prupe.5G070700_v2.0.a1 Prupe.7G038000_v2.0.a1 Prupe.7G164000_v2.0.a1 Prupe.8G056500_v2.0.a1
pyrus_communis pycom04g02040 pycom05g04600 pycom07g05610 pycom08g16130 pycom11g16470 pycom12g07830 pycom15g38190 pycom215g00040
rosa_chinensis RchiOBHm_Chr1g0348881 RchiOBHm_Chr5g0032951 RchiOBHm_Chr6g0273271 RchiOBHm_Chr7g0218191 RchiOBHm_Chr7g0223721
rosa_multiflora Rmu_co8286615.1_g000001 Rmu_sc0001354.1_g000002 Rmu_sc0001925.1_g000003 Rmu_sc0001925.1_g000005 Rmu_sc0002221.1_g000004 Rmu_sc0002316.1_g000096 Rmu_sc0003291.1_g000005 Rmu_sc0003413.1_g000005 Rmu_sc0003542.1_g000011 Rmu_sc0004035.1_g000003 Rmu_sc0004035.1_g000004 Rmu_sc0004145.1_g000016 Rmu_sc0004660.1_g000004 Rmu_sc0005999.1_g000006 Rmu_sc0006123.1_g000002 Rmu_sc0006586.1_g000006 Rmu_sc0006889.1_g000029 Rmu_sc0008035.1_g000014 Rmu_sc0011962.1_g000002 Rmu_sc0029950.1_g000001 Rmu_sc0042409.1_g000001 Rmu_ssc0000238.1_g000013
rosa_roxburghii Rroxscaffold_2G00137410 Rroxscaffold_4G00312010 Rroxscaffold_4G00320800 Rroxscaffold_5G00341720 Rroxscaffold_5G00353530 Rroxscaffold_5G00360290 Rroxscaffold_7G00214170 Rroxscaffold_7G00215920
rosa_rugosa Rorug02G0157000
rosa_samantha Rh4BG196500 Rh6CG346400 Rh7BG241100 Rh7DG316300
rosa_wichuraiana Rw1G003610 Rw7G012170 Rw7G030150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 711
AccB7I CCANNNNNTGG 1 cut(s) 652
AccBSI CCGCTC 1 cut(s) 946
AccII CGCG 1 cut(s) 1292
AciI CCGC 1 cut(s) 946
AclWI GGATC 1 cut(s) 1150
AcsI RAATTY 2 cut(s) 159, 949
AcuI CTGAAG 4 cut(s) 111, 497, 785, 1256
AfaI GTAC 1 cut(s) 310
AfiI CCNNNNNNNGG 2 cut(s) 56, 652
AflIII ACRYGT 1 cut(s) 1290
AgsI TTSAA 7 cut(s) 40, 206, 616, 792, 800, 1094, 1133
AjnI CCWGG 3 cut(s) 96, 425, 814
AluBI AGCT 7 cut(s) 385, 755, 856, 913, 976, 1073, 1153
AluI AGCT 7 cut(s) 385, 755, 856, 913, 976, 1073, 1153
Alw21I GWGCWC 1 cut(s) 387
Alw26I GTCTC 1 cut(s) 282
AlwI GGATC 1 cut(s) 1150
Ama87I CYCGRG 2 cut(s) 498, 1303
AoxI GGCC 2 cut(s) 264, 331
ApeKI GCWGC 3 cut(s) 33, 1070, 1150
ApoI RAATTY 2 cut(s) 159, 949
AseI ATTAAT 1 cut(s) 1044
Asp700I GAANNNNTTC 1 cut(s) 698
AspS9I GGNCC 3 cut(s) 331, 495, 1115
AsuHPI GGTGA 2 cut(s) 272, 796
AvaI CYCGRG 2 cut(s) 498, 1303
AvaII GGWCC 2 cut(s) 495, 1115
AxyI CCTNAGG 1 cut(s) 780
BanII GRGCYC 2 cut(s) 62, 387
Bbv12I GWGCWC 1 cut(s) 387
BbvI GCAGC 3 cut(s) 20, 1082, 1162
BccI CCATC 1 cut(s) 1272
BciT130I CCWGG 3 cut(s) 98, 427, 816
BcoDI GTCTC 1 cut(s) 282
BfaI CTAG 2 cut(s) 113, 752
BglI GCCNNNNNGGC 1 cut(s) 1229
BglII AGATCT 1 cut(s) 609
BisI GCNGC 3 cut(s) 34, 1071, 1151
BlsI GCNGC 3 cut(s) 35, 1072, 1152
Bme1390I CCNGG 3 cut(s) 98, 427, 816
Bme18I GGWCC 2 cut(s) 495, 1115
BmeT110I CYCGRG 2 cut(s) 498, 1303
BmgT120I GGNCC 3 cut(s) 331, 495, 1115
BmiI GGNNCC 3 cut(s) 332, 497, 894
BmrFI CCNGG 3 cut(s) 98, 427, 816
BmrI ACTGGG 1 cut(s) 577
BmsI GCATC 3 cut(s) 112, 118, 394
BmuI ACTGGG 1 cut(s) 577
BpuEI CTTGAG 2 cut(s) 269, 485
BsaJI CCNNGG 3 cut(s) 141, 1159, 1195
Bsc4I CCNNNNNNNGG 2 cut(s) 56, 652
Bse1I ACTGG 2 cut(s) 530, 572
Bse21I CCTNAGG 1 cut(s) 780
Bse3DI GCAATG 2 cut(s) 216, 377
BseBI CCWGG 3 cut(s) 98, 427, 816
BseDI CCNNGG 3 cut(s) 141, 1159, 1195
BseGI GGATG 3 cut(s) 10, 409, 1283
BseLI CCNNNNNNNGG 2 cut(s) 56, 652
BseMI GCAATG 2 cut(s) 216, 377
BseMII CTCAG 1 cut(s) 771
BseNI ACTGG 2 cut(s) 530, 572
BseRI GAGGAG 1 cut(s) 488
BseXI GCAGC 3 cut(s) 20, 1082, 1162
BseYI CCCAGC 1 cut(s) 1073
Bsh1236I CGCG 1 cut(s) 1292
BshFI GGCC 2 cut(s) 266, 333
BsiHKAI GWGCWC 1 cut(s) 387
BsiHKCI CYCGRG 2 cut(s) 498, 1303
BslFI GGGAC 1 cut(s) 481
BslI CCNNNNNNNGG 2 cut(s) 56, 652
BsmAI GTCTC 1 cut(s) 282
BsmFI GGGAC 1 cut(s) 481
BsmI GAATGC 1 cut(s) 1164
BsnI GGCC 2 cut(s) 266, 333
BsoBI CYCGRG 2 cut(s) 498, 1303
Bsp1286I GDGCHC 2 cut(s) 62, 387
Bsp1407I TGTACA 1 cut(s) 308
Bsp143I GATC 5 cut(s) 457, 609, 768, 934, 1155
BspACI CCGC 1 cut(s) 946
BspANI GGCC 2 cut(s) 266, 333
BspCNI CTCAG 1 cut(s) 772
BspFNI CGCG 1 cut(s) 1292
BspHI TCATGA 1 cut(s) 966
BspLI GGNNCC 3 cut(s) 332, 497, 894
BspPI GGATC 1 cut(s) 1150
BsrBI CCGCTC 1 cut(s) 946
BsrDI GCAATG 2 cut(s) 216, 377
BsrGI TGTACA 1 cut(s) 308
BsrI ACTGG 2 cut(s) 530, 572
BssECI CCNNGG 3 cut(s) 141, 1159, 1195
BssMI GATC 5 cut(s) 457, 609, 768, 934, 1155
BssT1I CCWWGG 3 cut(s) 141, 1159, 1195
Bst2UI CCWGG 3 cut(s) 98, 427, 816
Bst4CI ACNGT 6 cut(s) 236, 306, 342, 761, 868, 1027
Bst6I CTCTTC 1 cut(s) 219
BstAUI TGTACA 1 cut(s) 308
BstC8I GCNNGC 2 cut(s) 546, 605
BstDEI CTNAG 1 cut(s) 780
BstF5I GGATG 3 cut(s) 10, 409, 1283
BstFNI CGCG 1 cut(s) 1292
BstKTI GATC 5 cut(s) 460, 612, 771, 937, 1158
BstMAI GTCTC 1 cut(s) 282
BstMBI GATC 5 cut(s) 457, 609, 768, 934, 1155
BstMWI GCNNNNNNNGC 2 cut(s) 215, 1229
BstNI CCWGG 3 cut(s) 98, 427, 816
BstNSI RCATGY 1 cut(s) 155
BstSCI CCNGG 3 cut(s) 96, 425, 814
BstUI CGCG 1 cut(s) 1292
BstV1I GCAGC 3 cut(s) 20, 1082, 1162
BstX2I RGATCY 1 cut(s) 609
BstYI RGATCY 1 cut(s) 609
Bsu36I CCTNAGG 1 cut(s) 780
BsuRI GGCC 2 cut(s) 266, 333
BtsCI GGATG 3 cut(s) 10, 409, 1283
BtsIMutI CAGTG 1 cut(s) 512
Cac8I GCNNGC 2 cut(s) 546, 605
CciI TCATGA 1 cut(s) 966
Cfr13I GGNCC 3 cut(s) 331, 495, 1115
Csp6I GTAC 1 cut(s) 309
CviAII CATG 7 cut(s) 134, 152, 164, 434, 536, 967, 1184
CviQI GTAC 1 cut(s) 309
DdeI CTNAG 1 cut(s) 780
DpnI GATC 5 cut(s) 459, 611, 770, 936, 1157
DpnII GATC 5 cut(s) 457, 609, 768, 934, 1155
DrdI GACNNNNNNGTC 1 cut(s) 711
DseDI GACNNNNNNGTC 1 cut(s) 711
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
Ecl136II GAGCTC 1 cut(s) 385
Eco130I CCWWGG 3 cut(s) 141, 1159, 1195
Eco147I AGGCCT 1 cut(s) 266
Eco24I GRGCYC 2 cut(s) 62, 387
Eco47I GGWCC 2 cut(s) 495, 1115
Eco53kI GAGCTC 1 cut(s) 385
Eco57I CTGAAG 4 cut(s) 111, 497, 785, 1256
Eco81I CCTNAGG 1 cut(s) 780
Eco88I CYCGRG 2 cut(s) 498, 1303
EcoICRI GAGCTC 1 cut(s) 385
EcoO109I RGGNCCY 1 cut(s) 495
EcoRII CCWGG 3 cut(s) 96, 425, 814
EcoT14I CCWWGG 3 cut(s) 141, 1159, 1195
EcoT22I ATGCAT 1 cut(s) 864
EcoT38I GRGCYC 2 cut(s) 62, 387
ErhI CCWWGG 3 cut(s) 141, 1159, 1195
FaeI CATG 7 cut(s) 137, 155, 167, 437, 539, 970, 1187
FalI AAGNNNNNCTT 4 cut(s) 965, 997, 1220, 1252
FaqI GGGAC 1 cut(s) 481
FatI CATG 7 cut(s) 133, 151, 163, 433, 535, 966, 1183
FauNDI CATATG 1 cut(s) 931
Fnu4HI GCNGC 3 cut(s) 34, 1071, 1151
FokI GGATG 3 cut(s) 17, 416, 1290
FriOI GRGCYC 2 cut(s) 62, 387
Fsp4HI GCNGC 3 cut(s) 34, 1071, 1151
FspBI CTAG 2 cut(s) 113, 752
GluI GCNGC 3 cut(s) 34, 1071, 1151
GsaI CCCAGC 1 cut(s) 1077
HaeIII GGCC 2 cut(s) 266, 333
Hin1II CATG 7 cut(s) 137, 155, 167, 437, 539, 970, 1187
HincII GTYRAC 1 cut(s) 1243
HindII GTYRAC 1 cut(s) 1243
HindIII AAGCTT 2 cut(s) 911, 974
HinfI GANTC 3 cut(s) 364, 664, 1083
HphI GGTGA 2 cut(s) 272, 796
Hpy166II GTNNAC 4 cut(s) 309, 311, 1243, 1300
Hpy188I TCNGA 5 cut(s) 28, 130, 669, 704, 765
Hpy188III TCNNGA 7 cut(s) 113, 248, 395, 498, 515, 613, 967
Hpy8I GTNNAC 4 cut(s) 309, 311, 1243, 1300
HpyAV CCTTC 2 cut(s) 688, 1222
HpyCH4III ACNGT 6 cut(s) 236, 306, 342, 761, 868, 1027
HpyCH4IV ACGT 3 cut(s) 316, 681, 723
HpyCH4V TGCA 5 cut(s) 407, 607, 862, 986, 1150
HpyF10VI GCNNNNNNNGC 2 cut(s) 215, 1229
HpyF3I CTNAG 1 cut(s) 780
HpySE526I ACGT 3 cut(s) 316, 681, 723
Hsp92II CATG 7 cut(s) 137, 155, 167, 437, 539, 970, 1187
Kzo9I GATC 5 cut(s) 457, 609, 768, 934, 1155
LmnI GCTCC 5 cut(s) 75, 172, 382, 390, 892
Lsp1109I GCAGC 3 cut(s) 20, 1082, 1162
LweI GCATC 3 cut(s) 112, 118, 394
MaeI CTAG 2 cut(s) 113, 752
MaeII ACGT 3 cut(s) 316, 681, 723
MaeIII GTNAC 5 cut(s) 719, 838, 1018, 1027, 1286
MalI GATC 5 cut(s) 459, 611, 770, 936, 1157
MbiI CCGCTC 1 cut(s) 946
MboI GATC 5 cut(s) 457, 609, 768, 934, 1155
MboII GAAGA 6 cut(s) 236, 596, 682, 778, 947, 1262
MflI RGATCY 1 cut(s) 609
MhlI GDGCHC 2 cut(s) 62, 387
MluI ACGCGT 1 cut(s) 1290
MmeI TCCRAC 3 cut(s) 727, 1195, 1240
MnlI CCTC 9 cut(s) 74, 220, 244, 256, 277, 294, 466, 511, 775
Mph1103I ATGCAT 1 cut(s) 864
MroXI GAANNNNTTC 1 cut(s) 698
MseI TTAA 7 cut(s) 12, 321, 447, 633, 642, 888, 1044
MspA1I CMGCKG 2 cut(s) 1073, 1153
MspR9I CCNGG 3 cut(s) 98, 427, 816
Mva1269I GAATGC 1 cut(s) 1164
MvaI CCWGG 3 cut(s) 98, 427, 816
MvnI CGCG 1 cut(s) 1292
MwoI GCNNNNNNNGC 2 cut(s) 215, 1229
NdeI CATATG 1 cut(s) 931
NdeII GATC 5 cut(s) 457, 609, 768, 934, 1155
NlaIII CATG 7 cut(s) 137, 155, 167, 437, 539, 970, 1187
NlaIV GGNNCC 3 cut(s) 332, 497, 894
NmuCI GTSAC 3 cut(s) 838, 1018, 1286
NsiI ATGCAT 1 cut(s) 864
NspI RCATGY 1 cut(s) 155
PaeR7I CTCGAG 1 cut(s) 1303
PagI TCATGA 1 cut(s) 966
PceI AGGCCT 1 cut(s) 266
PcsI WCGNNNNNNNCGW 1 cut(s) 190
PctI GAATGC 1 cut(s) 1164
PdmI GAANNNNTTC 1 cut(s) 698
PfeI GAWTC 3 cut(s) 364, 664, 1083
PflMI CCANNNNNTGG 1 cut(s) 652
PfoI TCCNGGA 1 cut(s) 814
PkrI GCNGC 3 cut(s) 35, 1072, 1152
PpuMI RGGWCCY 1 cut(s) 495
PshBI ATTAAT 1 cut(s) 1044
Psp124BI GAGCTC 1 cut(s) 387
Psp5II RGGWCCY 1 cut(s) 495
Psp6I CCWGG 3 cut(s) 96, 425, 814
PspFI CCCAGC 1 cut(s) 1073
PspGI CCWGG 3 cut(s) 96, 425, 814
PspN4I GGNNCC 3 cut(s) 332, 497, 894
PspPI GGNCC 3 cut(s) 331, 495, 1115
PspPPI RGGWCCY 1 cut(s) 495
PspXI VCTCGAGB 1 cut(s) 1303
PsuI RGATCY 1 cut(s) 609
PvuII CAGCTG 2 cut(s) 1073, 1153
RsaI GTAC 1 cut(s) 310
RsaNI GTAC 1 cut(s) 309
SacI GAGCTC 1 cut(s) 387
SaqAI TTAA 7 cut(s) 12, 321, 447, 633, 642, 888, 1044
SatI GCNGC 3 cut(s) 34, 1071, 1151
Sau3AI GATC 5 cut(s) 457, 609, 768, 934, 1155
Sau96I GGNCC 3 cut(s) 331, 495, 1115
ScrFI CCNGG 3 cut(s) 98, 427, 816
SduI GDGCHC 2 cut(s) 62, 387
SfaNI GCATC 3 cut(s) 112, 118, 394
Sfr274I CTCGAG 1 cut(s) 1303
SinI GGWCC 2 cut(s) 495, 1115
SlaI CTCGAG 1 cut(s) 1303
SmlI CTYRAG 3 cut(s) 248, 464, 1303
SmoI CTYRAG 3 cut(s) 248, 464, 1303
SseBI AGGCCT 1 cut(s) 266
SsiI CCGC 1 cut(s) 946
SspI AATATT 1 cut(s) 1129
SspMI CTAG 2 cut(s) 113, 752
SstI GAGCTC 1 cut(s) 387
StuI AGGCCT 1 cut(s) 266
StyD4I CCNGG 3 cut(s) 96, 425, 814
StyI CCWWGG 3 cut(s) 141, 1159, 1195
TaaI ACNGT 6 cut(s) 236, 306, 342, 761, 868, 1027
TaiI ACGT 3 cut(s) 319, 684, 726
TaqI TCGA 2 cut(s) 937, 1304
TatI WGTACW 1 cut(s) 308
TfiI GAWTC 3 cut(s) 364, 664, 1083
Tru1I TTAA 7 cut(s) 12, 321, 447, 633, 642, 888, 1044
Tru9I TTAA 7 cut(s) 12, 321, 447, 633, 642, 888, 1044
TscAI CASTG 1 cut(s) 512
TseFI GTSAC 3 cut(s) 838, 1018, 1286
TseI GCWGC 3 cut(s) 33, 1070, 1150
Tsp45I GTSAC 3 cut(s) 838, 1018, 1286
TspDTI ATGAA 6 cut(s) 152, 188, 237, 450, 983, 1077
TspRI CASTG 1 cut(s) 512
Van91I CCANNNNNTGG 1 cut(s) 652
VpaK11BI GGWCC 2 cut(s) 495, 1115
VspI ATTAAT 1 cut(s) 1044
XapI RAATTY 2 cut(s) 159, 949
XbaI TCTAGA 1 cut(s) 112
XceI RCATGY 1 cut(s) 155
XcmI CCANNNNNNNNNTGG 1 cut(s) 341
XhoI CTCGAG 1 cut(s) 1303
XmnI GAANNNNTTC 1 cut(s) 698
XspI CTAG 2 cut(s) 113, 752
Zsp2I ATGCAT 1 cut(s) 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.