pycom07g05610

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000842
Physical Location & Seq
Reverse (-)
4893170 .. 4893544
375 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g05610.1

Sequence Viewer

Length: 375 bp
ATGACTCAGCTCTGGCTTATTAGCCTCTGCAATGTACTATTTAGGATAGTTTCCAAGGTAATTGCGAACCGCCTCAAGCCATTACTCCATCATGTGATCTCCCATCACCAGAGCGCCTTTGTCCCAGGCCGACATATTTCGGATAACATAATTCTGGCTATGGAGTTATCTCACTTTATGTTCAAGTGCCGCATGGGCAAAAAAGGTTTCTTATCATGGAAACTTGACATGAGCAAAGCTTATGATCGAATTGAGTGGGGTTATTTGAGGAATGTTCTTCTCCGACTTAGTTTCTGCCGTAAGGGGATCGATCTGATTATGACTTGTGTCTCGACAGTTTCTTACACTTTTCTAGTTAATGGCATTCCAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

125

Amino Acids

14.41

Weight (kDa)

10.04

Isoelectric Point (pI)

26.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 6 - 110 5e-11 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000842)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15420 FvH4_2g00771 FvH4_3g29363
malus_domestica MD02G1293300.v1.1 MD06G1023000.v1.1 MD09G1232900.v1.1
prunus_persica Prupe.1G181500_v2.0.a1 Prupe.1G572100_v2.0.a1 Prupe.2G095500_v2.0.a1 Prupe.3G150400_v2.0.a1 Prupe.3G156200_v2.0.a1 Prupe.4G274400_v2.0.a1 Prupe.5G070700_v2.0.a1 Prupe.7G038000_v2.0.a1 Prupe.7G164000_v2.0.a1 Prupe.8G056500_v2.0.a1
pyrus_communis pycom04g02040 pycom05g04600 pycom07g05610 pycom08g16130 pycom11g16470 pycom12g07830 pycom15g38190 pycom215g00040
rosa_chinensis RchiOBHm_Chr1g0348881 RchiOBHm_Chr5g0032951 RchiOBHm_Chr6g0273271 RchiOBHm_Chr7g0218191 RchiOBHm_Chr7g0223721
rosa_multiflora Rmu_co8286615.1_g000001 Rmu_sc0001354.1_g000002 Rmu_sc0001925.1_g000003 Rmu_sc0001925.1_g000005 Rmu_sc0002221.1_g000004 Rmu_sc0002316.1_g000096 Rmu_sc0003291.1_g000005 Rmu_sc0003413.1_g000005 Rmu_sc0003542.1_g000011 Rmu_sc0004035.1_g000003 Rmu_sc0004035.1_g000004 Rmu_sc0004145.1_g000016 Rmu_sc0004660.1_g000004 Rmu_sc0005999.1_g000006 Rmu_sc0006123.1_g000002 Rmu_sc0006586.1_g000006 Rmu_sc0006889.1_g000029 Rmu_sc0008035.1_g000014 Rmu_sc0011962.1_g000002 Rmu_sc0029950.1_g000001 Rmu_sc0042409.1_g000001 Rmu_ssc0000238.1_g000013
rosa_roxburghii Rroxscaffold_2G00137410 Rroxscaffold_4G00312010 Rroxscaffold_4G00320800 Rroxscaffold_5G00341720 Rroxscaffold_5G00353530 Rroxscaffold_5G00360290 Rroxscaffold_7G00214170 Rroxscaffold_7G00215920
rosa_rugosa Rorug02G0157000
rosa_samantha Rh4BG196500 Rh6CG346400 Rh7BG241100 Rh7DG316300
rosa_wichuraiana Rw1G003610 Rw7G012170 Rw7G030150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 70, 190
AclWI GGATC 1 cut(s) 314
AfaI GTAC 1 cut(s) 36
AgsI TTSAA 1 cut(s) 184
AjnI CCWGG 1 cut(s) 124
AluBI AGCT 2 cut(s) 10, 239
AluI AGCT 2 cut(s) 10, 239
Alw26I GTCTC 1 cut(s) 334
AlwI GGATC 1 cut(s) 314
AoxI GGCC 1 cut(s) 127
AspLEI GCGC 1 cut(s) 116
AsuHPI GGTGA 1 cut(s) 98
BccI CCATC 2 cut(s) 96, 111
BceAI ACGGC 1 cut(s) 282
BciT130I CCWGG 1 cut(s) 126
BcoDI GTCTC 1 cut(s) 334
BfaI CTAG 1 cut(s) 353
BfoI RGCGCY 1 cut(s) 117
BglI GCCNNNNNGGC 1 cut(s) 195
BisI GCNGC 1 cut(s) 190
BlsI GCNGC 1 cut(s) 191
Bme1390I CCNGG 1 cut(s) 126
BmrFI CCNGG 1 cut(s) 126
BoxI GACNNNNGTC 1 cut(s) 326
BpuEI CTTGAG 1 cut(s) 59
Bsa29I ATCGAT 1 cut(s) 309
BsaJI CCNNGG 2 cut(s) 54, 124
Bse3DI GCAATG 1 cut(s) 37
BseBI CCWGG 1 cut(s) 126
BseCI ATCGAT 1 cut(s) 309
BseDI CCNNGG 2 cut(s) 54, 124
BseMI GCAATG 1 cut(s) 37
BseMII CTCAG 1 cut(s) 20
BshFI GGCC 1 cut(s) 129
BshVI ATCGAT 1 cut(s) 309
BslFI GGGAC 1 cut(s) 107
BsmAI GTCTC 1 cut(s) 334
BsmFI GGGAC 1 cut(s) 107
BsmI GAATGC 1 cut(s) 363
BsnI GGCC 1 cut(s) 129
Bsp143I GATC 4 cut(s) 96, 244, 306, 310
BspACI CCGC 2 cut(s) 70, 190
BspANI GGCC 1 cut(s) 129
BspCNI CTCAG 1 cut(s) 19
BspDI ATCGAT 1 cut(s) 309
BspPI GGATC 1 cut(s) 314
BsrDI GCAATG 1 cut(s) 37
BssECI CCNNGG 2 cut(s) 54, 124
BssMI GATC 4 cut(s) 96, 244, 306, 310
BssT1I CCWWGG 1 cut(s) 54
Bst2UI CCWGG 1 cut(s) 126
Bst4CI ACNGT 1 cut(s) 337
BstDEI CTNAG 2 cut(s) 6, 287
BstH2I RGCGCY 1 cut(s) 117
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 4 cut(s) 99, 247, 309, 313
BstMAI GTCTC 1 cut(s) 334
BstMBI GATC 4 cut(s) 96, 244, 306, 310
BstMWI GCNNNNNNNGC 1 cut(s) 195
BstNI CCWGG 1 cut(s) 126
BstPAI GACNNNNGTC 1 cut(s) 326
BstSCI CCNGG 1 cut(s) 124
Bsu15I ATCGAT 1 cut(s) 309
BsuRI GGCC 1 cut(s) 129
BsuTUI ATCGAT 1 cut(s) 309
CfoI GCGC 1 cut(s) 116
ClaI ATCGAT 1 cut(s) 309
Csp6I GTAC 1 cut(s) 35
CviAII CATG 4 cut(s) 92, 193, 216, 229
CviJI RGCY 7 cut(s) 10, 16, 24, 79, 129, 158, 239
CviKI_1 RGCY 7 cut(s) 10, 16, 24, 79, 129, 158, 239
CviQI GTAC 1 cut(s) 35
DdeI CTNAG 2 cut(s) 6, 287
DpnI GATC 4 cut(s) 98, 246, 308, 312
DpnII GATC 4 cut(s) 96, 244, 306, 310
Eco130I CCWWGG 1 cut(s) 54
EcoRII CCWGG 1 cut(s) 124
EcoT14I CCWWGG 1 cut(s) 54
ErhI CCWWGG 1 cut(s) 54
FaeI CATG 4 cut(s) 95, 196, 219, 232
FaqI GGGAC 1 cut(s) 107
FatI CATG 4 cut(s) 91, 192, 215, 228
Fnu4HI GCNGC 1 cut(s) 190
Fsp4HI GCNGC 1 cut(s) 190
FspBI CTAG 1 cut(s) 353
GlaI GCGC 1 cut(s) 115
GluI GCNGC 1 cut(s) 190
HaeII RGCGCY 1 cut(s) 117
HaeIII GGCC 1 cut(s) 129
HhaI GCGC 1 cut(s) 116
Hin1II CATG 4 cut(s) 95, 196, 219, 232
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HindIII AAGCTT 1 cut(s) 237
HinfI GANTC 1 cut(s) 4
HphI GGTGA 1 cut(s) 98
Hpy188I TCNGA 3 cut(s) 142, 284, 315
Hpy188III TCNNGA 1 cut(s) 331
HpyCH4III ACNGT 1 cut(s) 337
HpyCH4V TGCA 1 cut(s) 30
HpyF10VI GCNNNNNNNGC 1 cut(s) 195
HpyF3I CTNAG 2 cut(s) 6, 287
Hsp92II CATG 4 cut(s) 95, 196, 219, 232
HspAI GCGC 1 cut(s) 114
Kzo9I GATC 4 cut(s) 96, 244, 306, 310
LpnPI CCDG 4 cut(s) 111, 122, 138, 140
MaeI CTAG 1 cut(s) 353
MalI GATC 4 cut(s) 98, 246, 308, 312
MboI GATC 4 cut(s) 96, 244, 306, 310
MboII GAAGA 1 cut(s) 269
MluCI AATT 3 cut(s) 60, 150, 249
MmeI TCCRAC 1 cut(s) 307
MnlI CCTC 3 cut(s) 35, 83, 261
MseI TTAA 1 cut(s) 357
MspR9I CCNGG 1 cut(s) 126
Mva1269I GAATGC 1 cut(s) 363
MvaI CCWGG 1 cut(s) 126
MwoI GCNNNNNNNGC 1 cut(s) 195
NdeII GATC 4 cut(s) 96, 244, 306, 310
NlaIII CATG 4 cut(s) 95, 196, 219, 232
PctI GAATGC 1 cut(s) 363
PkrI GCNGC 1 cut(s) 191
PshAI GACNNNNGTC 1 cut(s) 326
Psp6I CCWGG 1 cut(s) 124
PspGI CCWGG 1 cut(s) 124
RsaI GTAC 1 cut(s) 36
RsaNI GTAC 1 cut(s) 35
SaqAI TTAA 1 cut(s) 357
SatI GCNGC 1 cut(s) 190
Sau3AI GATC 4 cut(s) 96, 244, 306, 310
ScrFI CCNGG 1 cut(s) 126
SetI ASST 4 cut(s) 12, 60, 208, 241
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
Sse9I AATT 3 cut(s) 60, 150, 249
SsiI CCGC 2 cut(s) 70, 190
SspMI CTAG 1 cut(s) 353
StyD4I CCNGG 1 cut(s) 124
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 1 cut(s) 337
TaqI TCGA 3 cut(s) 247, 309, 332
TasI AATT 3 cut(s) 60, 150, 249
TatI WGTACW 1 cut(s) 34
TauI GCSGC 1 cut(s) 192
Tru1I TTAA 1 cut(s) 357
Tru9I TTAA 1 cut(s) 357
XspI CTAG 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.