pycom11g16470

ribonuclease H protein At1g65750

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0000842
Physical Location & Seq
Reverse (-)
18069395 .. 18069973
579 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g16470.1

Sequence Viewer

Length: 579 bp
ATGGCAGATTCTTGGGGTTGTGAGTTGGGTAGTTGGCCAATAAAGTACTTGGGTCTCCCTCTTGGGGGTAGGCCAATAACACTTAGGTTTTGGGATCCAGTGGTAGAAAAAAAATGGAAAAAAAGACTTCAAAGTTGGAAGAAGGCCTTCTTATCTAGAAGAGGTAGATTAACTTTGATTCAATCTGTTTTGGGAAGCTTGCCAATCTACTACATGTCCTTGTTCAAAATTCCTTGCGGGGTGATTGGACGCCTAGAAAAACTAATGAAAGGGTTTCTTTGGGAAGGGGTGGAGGAAGGAAAGAAAAACCACCTGGTGAAATGGGAGATAGTTATTAAAAGTAAGGAGGAAGGGGGTTTAGTTGTGGGGAATTTGAGGAATCAGAATGAAGCGTTACTGGCTACGTGGTTGTGGAGATTCCTAAAGGAACCAAATTCTCTCTGGCACAAGGTGATAAGAAGCAAGTATGGGCTGCAGGATAATGGGTGGAATGCATCCTCCATTGCGGGGTTCAAGTCACAGCCCCTGGAAAGATATTTCGAGTGGTCTCCAATCGTTTCTACAATGCTGCAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

193

Amino Acids

22.26

Weight (kDa)

9.94

Isoelectric Point (pI)

49.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000842)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15420 FvH4_2g00771 FvH4_3g29363
malus_domestica MD02G1293300.v1.1 MD06G1023000.v1.1 MD09G1232900.v1.1
prunus_persica Prupe.1G181500_v2.0.a1 Prupe.1G572100_v2.0.a1 Prupe.2G095500_v2.0.a1 Prupe.3G150400_v2.0.a1 Prupe.3G156200_v2.0.a1 Prupe.4G274400_v2.0.a1 Prupe.5G070700_v2.0.a1 Prupe.7G038000_v2.0.a1 Prupe.7G164000_v2.0.a1 Prupe.8G056500_v2.0.a1
pyrus_communis pycom04g02040 pycom05g04600 pycom07g05610 pycom08g16130 pycom11g16470 pycom12g07830 pycom15g38190 pycom215g00040
rosa_chinensis RchiOBHm_Chr1g0348881 RchiOBHm_Chr5g0032951 RchiOBHm_Chr6g0273271 RchiOBHm_Chr7g0218191 RchiOBHm_Chr7g0223721
rosa_multiflora Rmu_co8286615.1_g000001 Rmu_sc0001354.1_g000002 Rmu_sc0001925.1_g000003 Rmu_sc0001925.1_g000005 Rmu_sc0002221.1_g000004 Rmu_sc0002316.1_g000096 Rmu_sc0003291.1_g000005 Rmu_sc0003413.1_g000005 Rmu_sc0003542.1_g000011 Rmu_sc0004035.1_g000003 Rmu_sc0004035.1_g000004 Rmu_sc0004145.1_g000016 Rmu_sc0004660.1_g000004 Rmu_sc0005999.1_g000006 Rmu_sc0006123.1_g000002 Rmu_sc0006586.1_g000006 Rmu_sc0006889.1_g000029 Rmu_sc0008035.1_g000014 Rmu_sc0011962.1_g000002 Rmu_sc0029950.1_g000001 Rmu_sc0042409.1_g000001 Rmu_ssc0000238.1_g000013
rosa_roxburghii Rroxscaffold_2G00137410 Rroxscaffold_4G00312010 Rroxscaffold_4G00320800 Rroxscaffold_5G00341720 Rroxscaffold_5G00353530 Rroxscaffold_5G00360290 Rroxscaffold_7G00214170 Rroxscaffold_7G00215920
rosa_rugosa Rorug02G0157000
rosa_samantha Rh4BG196500 Rh6CG346400 Rh7BG241100 Rh7DG316300
rosa_wichuraiana Rw1G003610 Rw7G012170 Rw7G030150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 237, 506
AclWI GGATC 2 cut(s) 89, 102
AcoI YGGCCR 1 cut(s) 35
AcsI RAATTY 3 cut(s) 228, 370, 433
AcyI GRCGYC 1 cut(s) 250
AdeI CACNNNGTG 2 cut(s) 316, 451
AfaI GTAC 1 cut(s) 47
AfiI CCNNNNNNNGG 3 cut(s) 64, 65, 507
AflIII ACRYGT 1 cut(s) 213
AgsI TTSAA 4 cut(s) 131, 182, 226, 514
AjnI CCWGG 2 cut(s) 312, 525
AluBI AGCT 1 cut(s) 198
AluI AGCT 1 cut(s) 198
Alw26I GTCTC 2 cut(s) 59, 552
AlwI GGATC 2 cut(s) 89, 102
AlwNI CAGNNNCTG 1 cut(s) 526
AoxI GGCC 3 cut(s) 35, 71, 144
ApeKI GCWGC 2 cut(s) 472, 568
ApoI RAATTY 3 cut(s) 228, 370, 433
Asp700I GAANNNNTTC 1 cut(s) 146
AsuHPI GGTGA 3 cut(s) 253, 328, 463
BalI TGGCCA 1 cut(s) 37
BamHI GGATCC 1 cut(s) 94
BbvI GCAGC 2 cut(s) 459, 555
BciT130I CCWGG 2 cut(s) 314, 527
BcoDI GTCTC 2 cut(s) 59, 552
BfaI CTAG 2 cut(s) 156, 254
BfmI CTRYAG 1 cut(s) 473
BisI GCNGC 2 cut(s) 473, 569
BlsI GCNGC 2 cut(s) 474, 570
BmcAI AGTACT 1 cut(s) 47
Bme1390I CCNGG 2 cut(s) 314, 527
BmiI GGNNCC 2 cut(s) 96, 429
BmrFI CCNGG 2 cut(s) 314, 527
BmsI GCATC 1 cut(s) 503
BsaAI YACGTR 1 cut(s) 405
BsaHI GRCGYC 1 cut(s) 250
BsaI GGTCTC 2 cut(s) 59, 552
BsaJI CCNNGG 1 cut(s) 525
Bsc4I CCNNNNNNNGG 3 cut(s) 64, 65, 507
Bse1I ACTGG 2 cut(s) 98, 402
Bse3DI GCAATG 1 cut(s) 501
BseBI CCWGG 2 cut(s) 314, 527
BseDI CCNNGG 1 cut(s) 525
BseGI GGATG 1 cut(s) 494
BseLI CCNNNNNNNGG 3 cut(s) 64, 65, 507
BseMI GCAATG 1 cut(s) 501
BseNI ACTGG 2 cut(s) 98, 402
BseXI GCAGC 2 cut(s) 459, 555
BshFI GGCC 3 cut(s) 37, 73, 146
BslI CCNNNNNNNGG 3 cut(s) 64, 65, 507
BsmAI GTCTC 2 cut(s) 59, 552
BsmI GAATGC 1 cut(s) 496
BsnI GGCC 3 cut(s) 37, 73, 146
Bso31I GGTCTC 2 cut(s) 59, 552
Bsp143I GATC 1 cut(s) 94
BspACI CCGC 2 cut(s) 237, 506
BspANI GGCC 3 cut(s) 37, 73, 146
BspLI GGNNCC 2 cut(s) 96, 429
BspMAI CTGCAG 1 cut(s) 477
BspPI GGATC 2 cut(s) 89, 102
BspTNI GGTCTC 2 cut(s) 59, 552
BsrDI GCAATG 1 cut(s) 501
BsrI ACTGG 2 cut(s) 98, 402
BssECI CCNNGG 1 cut(s) 525
BssMI GATC 1 cut(s) 94
BssNI GRCGYC 1 cut(s) 250
Bst2UI CCWGG 2 cut(s) 314, 527
Bst6I CTCTTC 1 cut(s) 154
BstACI GRCGYC 1 cut(s) 250
BstBAI YACGTR 1 cut(s) 405
BstC8I GCNNGC 1 cut(s) 200
BstDEI CTNAG 1 cut(s) 83
BstF5I GGATG 1 cut(s) 494
BstKTI GATC 1 cut(s) 97
BstMAI GTCTC 2 cut(s) 59, 552
BstMBI GATC 1 cut(s) 94
BstMWI GCNNNNNNNGC 1 cut(s) 398
BstNI CCWGG 2 cut(s) 314, 527
BstNSI RCATGY 1 cut(s) 217
BstSCI CCNGG 2 cut(s) 312, 525
BstSFI CTRYAG 1 cut(s) 473
BstV1I GCAGC 2 cut(s) 459, 555
BstX2I RGATCY 1 cut(s) 94
BstYI RGATCY 1 cut(s) 94
BsuRI GGCC 3 cut(s) 37, 73, 146
BtsCI GGATG 1 cut(s) 494
BtsIMutI CAGTG 1 cut(s) 105
Cac8I GCNNGC 1 cut(s) 200
CaiI CAGNNNCTG 1 cut(s) 526
CseI GACGC 1 cut(s) 258
CsiI ACCWGGT 1 cut(s) 312
Csp6I GTAC 1 cut(s) 46
CviAII CATG 1 cut(s) 214
CviJI RGCY 7 cut(s) 37, 73, 146, 198, 401, 472, 523
CviKI_1 RGCY 7 cut(s) 37, 73, 146, 198, 401, 472, 523
CviQI GTAC 1 cut(s) 46
DdeI CTNAG 1 cut(s) 83
DpnI GATC 1 cut(s) 96
DpnII GATC 1 cut(s) 94
DraIII CACNNNGTG 2 cut(s) 316, 451
EaeI YGGCCR 1 cut(s) 35
Eam1104I CTCTTC 1 cut(s) 154
EarI CTCTTC 1 cut(s) 154
Eco147I AGGCCT 1 cut(s) 146
Eco31I GGTCTC 2 cut(s) 59, 552
EcoRII CCWGG 2 cut(s) 312, 525
EcoT22I ATGCAT 1 cut(s) 496
FaeI CATG 1 cut(s) 217
FaiI YATR 2 cut(s) 215, 468
FalI AAGNNNNNCTT 6 cut(s) 131, 163, 134, 166, 261, 293
FatI CATG 1 cut(s) 213
FauI CCCGC 2 cut(s) 230, 499
Fnu4HI GCNGC 2 cut(s) 473, 569
FokI GGATG 1 cut(s) 481
Fsp4HI GCNGC 2 cut(s) 473, 569
FspBI CTAG 2 cut(s) 156, 254
GluI GCNGC 2 cut(s) 473, 569
HaeIII GGCC 3 cut(s) 37, 73, 146
HgaI GACGC 1 cut(s) 258
Hin1I GRCGYC 1 cut(s) 250
Hin1II CATG 1 cut(s) 217
HindIII AAGCTT 1 cut(s) 196
HinfI GANTC 4 cut(s) 8, 178, 379, 417
HphI GGTGA 3 cut(s) 253, 328, 463
Hpy188I TCNGA 1 cut(s) 384
Hpy188III TCNNGA 1 cut(s) 156
HpyAV CCTTC 5 cut(s) 136, 157, 278, 290, 344
HpyCH4IV ACGT 1 cut(s) 404
HpyCH4V TGCA 3 cut(s) 475, 494, 571
HpyF10VI GCNNNNNNNGC 1 cut(s) 398
HpyF3I CTNAG 1 cut(s) 83
HpySE526I ACGT 1 cut(s) 404
Hsp92I GRCGYC 1 cut(s) 250
Hsp92II CATG 1 cut(s) 217
Kzo9I GATC 1 cut(s) 94
LpnPI CCDG 8 cut(s) 111, 299, 326, 383, 427, 461, 512, 539
Lsp1109I GCAGC 2 cut(s) 459, 555
LweI GCATC 1 cut(s) 503
MabI ACCWGGT 1 cut(s) 312
MaeI CTAG 2 cut(s) 156, 254
MaeII ACGT 1 cut(s) 404
MaeIII GTNAC 2 cut(s) 393, 516
MalI GATC 1 cut(s) 96
MboI GATC 1 cut(s) 94
MboII GAAGA 2 cut(s) 151, 171
MflI RGATCY 1 cut(s) 94
MlsI TGGCCA 1 cut(s) 37
MluCI AATT 3 cut(s) 228, 370, 433
MluNI TGGCCA 1 cut(s) 37
MmeI TCCRAC 1 cut(s) 116
MnlI CCTC 6 cut(s) 69, 155, 286, 340, 369, 508
Mox20I TGGCCA 1 cut(s) 37
Mph1103I ATGCAT 1 cut(s) 496
MroXI GAANNNNTTC 1 cut(s) 146
MscI TGGCCA 1 cut(s) 37
MseI TTAA 2 cut(s) 170, 336
Msp20I TGGCCA 1 cut(s) 37
MspR9I CCNGG 2 cut(s) 314, 527
Mva1269I GAATGC 1 cut(s) 496
MvaI CCWGG 2 cut(s) 314, 527
MwoI GCNNNNNNNGC 1 cut(s) 398
NdeII GATC 1 cut(s) 94
NlaIII CATG 1 cut(s) 217
NlaIV GGNNCC 2 cut(s) 96, 429
NmuCI GTSAC 1 cut(s) 516
NsiI ATGCAT 1 cut(s) 496
NspI RCATGY 1 cut(s) 217
PceI AGGCCT 1 cut(s) 146
PciI ACATGT 1 cut(s) 213
PctI GAATGC 1 cut(s) 496
PdmI GAANNNNTTC 1 cut(s) 146
PfeI GAWTC 4 cut(s) 8, 178, 379, 417
PkrI GCNGC 2 cut(s) 474, 570
Ppu21I YACGTR 1 cut(s) 405
PscI ACATGT 1 cut(s) 213
Psp6I CCWGG 2 cut(s) 312, 525
PspGI CCWGG 2 cut(s) 312, 525
PspN4I GGNNCC 2 cut(s) 96, 429
PstI CTGCAG 1 cut(s) 477
PstNI CAGNNNCTG 1 cut(s) 526
PsuI RGATCY 1 cut(s) 94
RsaI GTAC 1 cut(s) 47
RsaNI GTAC 1 cut(s) 46
SaqAI TTAA 2 cut(s) 170, 336
SatI GCNGC 2 cut(s) 473, 569
Sau3AI GATC 1 cut(s) 94
ScaI AGTACT 1 cut(s) 47
ScrFI CCNGG 2 cut(s) 314, 527
SetI ASST 6 cut(s) 89, 166, 200, 315, 407, 453
SexAI ACCWGGT 1 cut(s) 312
SfaNI GCATC 1 cut(s) 503
SfcI CTRYAG 1 cut(s) 473
Sse9I AATT 3 cut(s) 228, 370, 433
SseBI AGGCCT 1 cut(s) 146
SsiI CCGC 2 cut(s) 237, 506
SspMI CTAG 2 cut(s) 156, 254
StuI AGGCCT 1 cut(s) 146
StyD4I CCNGG 2 cut(s) 312, 525
TaiI ACGT 1 cut(s) 407
TaqI TCGA 1 cut(s) 540
TasI AATT 3 cut(s) 228, 370, 433
TatI WGTACW 1 cut(s) 45
TfiI GAWTC 4 cut(s) 8, 178, 379, 417
Tru1I TTAA 2 cut(s) 170, 336
Tru9I TTAA 2 cut(s) 170, 336
TscAI CASTG 1 cut(s) 105
TseFI GTSAC 1 cut(s) 516
TseI GCWGC 2 cut(s) 472, 568
Tsp45I GTSAC 1 cut(s) 516
TspDTI ATGAA 2 cut(s) 281, 402
TspRI CASTG 1 cut(s) 105
XapI RAATTY 3 cut(s) 228, 370, 433
XbaI TCTAGA 1 cut(s) 155
XceI RCATGY 1 cut(s) 217
XcmI CCANNNNNNNNNTGG 1 cut(s) 438
XmnI GAANNNNTTC 1 cut(s) 146
XspI CTAG 2 cut(s) 156, 254
ZrmI AGTACT 1 cut(s) 47
Zsp2I ATGCAT 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.