MD06G1023000.v1.1

ribonuclease H protein At1g65750

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000842
Physical Location & Seq
Reverse (-)
2882218 .. 2885522
3305 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1023000.v1.1.491

Sequence Viewer

Length: 2220 bp
ATGTTTACCTTTTTCATCATGTCAAATAAAAGTCTGCTGCAAATTTTAAGTATAAAGGTTTTACCTGTCTACTTAAAAGTGACGGACTATAGGCCCATAAGTTTAGTAACTGGATTGTATAAAATTATAGCCAAAACACTGGCTTCAAGATTGAAGGAGGTTTTGGATACCACTGTCTCTCCGAATCAGGGGGCGTTCGTCAAAGATAGACAAATTTTGGATGCTGTCCTCATTGCAAATGAAGTGGTAGAAGAGGTGAGGCAGAAAAAAGAGAAGGGATTGGTGCTTAAGATCGACTTTGAAAAAGCGTATGATCATGTGGAATGGAGATTTCTGGAAGAAGTACTACAAAGAAAAGGTTTTGGCAATAGATGGAGGAAATGGATGCAAGGATGTTTAAGCTCTGCAAATTTCTCCGTTTTGATCAATGGAAGGCCAAGGGGAAAATTTCAAGCTTCAAGAGGTTTGAGACAAGGCGATCCACTGTCACCATTTCTTTTCACCTTAGTAGTCGATGTTCTGAGCAGGTTAATGGAGAAGGCACAAGAAACTGATCTCATCAAAGGCTTGTGTATCGGCCAAGAAAAGGTGGAGATATCTCATCTCCAATTTGCCGACGACACCATTTTCTTCTTGACAGAAGACGAGGAAGTTTGGAACAACCTAATCCAAGTGCTTAATTTATTTTGTTCGGTCTCAGGATTGAAAATCAACAAAGCTAAGTGCTTTTTGGCTGGGATAAATTCAGACAATGAGAAACTAAACAGGCTGGCAGTTTCTTGGGGGTGCGAGGTAGGGTGCTGGCCGATAAAGTATTTGGGGCTTCCCCTCGGAGGAAGGCCAAGAGCAATAAAATTTTGGGATCCAGTGGTAGAAACAATGGAAAGAAGACTTCAAAGTTGGAAGAAGGCTTTCCTATCCAGAGGGGGTAAATTAACTCTAATTCAATCAGTCCTGGGAAGCATGCCGATTTACTATATGTCCTTGTTCAAAATTCCATGCGGGGTAAGAGGACGTATAGAAAGATTAATGAAGGGGTTTCTATGGGAAGGCATGGAGGAAGGTAAGAAAACACACTTGGCCAAGTGGGAGTTAGTCACTAAAAACAAAGAGGAAGGAGGTCTAGGGGTGGGGAACTTGAGGAATCAGAACGAAGCATTATTGGCTAAGTGGTTGTGGAGATTCCCAAGGGAGTCACAGTCTCTGTGGCACAAGGTGATAAAAAGTAAGTATGGGCTACAAGCTAATGGATGGGAAGCACTTCCTCATGGGAGGGTTTCAAGTCGAAGCCCATGGAAAGACATTTCGAGTGGTTCTCAACAGTTTCTTAGATGCTGCACGTTTGAGGTGGGCAACGGTGAGAGGGTGAGGTTTTGGGAGGATGGATGGGTAACAGGGGGACCGTTGAAGGAGCAATTCCCCAGACTATTCCTTCTATCGAGGAAGCATAATCAAAACATCTCGAGCTTCGTGGAAGTTTCAGCAAATCCTTTGTGTTGGAACTTTGATTTTAGGAGAAACTTGAATGAGATGGAGATAGTAGAGGTGGCCACATTATTACAGAAATTGGAAGAGGTTCGATTGTCTCCATTAAAGAGGGATATCAGAAGGTGGAACCTGGAAGCTTCAGGTTTGTTCTCATGCAAATCCTATCGTTCATTACGGAGCAACGATGGGAGTGTGCACTATTTTCCACCCTACCCACAGATTTGGAAATCAAAGGTTCCTCCGAAGGTTAAAATACTTGTATGGCTTGTGGCCAATGGGAATCTAAACACATGTGACAGAATTCAAAGGAGAAACCCTCTTATGTGCTTATCACCACATTGGTGTAGCCTGTGCAAAGCCAAGGAGGAGAGTGTAAATCACATATTTCTTCATTGCTCCTACTCGATCCAACTGTGGTGGAAATTGTTTCAGGAGGTTAAAGCAAGTTGGGTCATTCCTAAAGGGTGTTTCGAGCTACTAAGCACAAATTTTAAGGCACTTGGAAAAAGGAAAAAATCCAAAGTTTTGTGGGGTTGCTTGGTATCGGCAATTTTCTGGAATATATGGCTGGAACGTAACAAGAGGATTTTTGAAGACTATACTGGAGTGGGGGCAGAAGAACTTTGGGGGAGAGTAAAATATTGGGCAGCTTTTTGGGCTTCGGTAACTAAAGAGTTTAAGAATGTTTCTCTATCTCAAATACTATGGGATGTGTTAGCTGCAGTTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

740

Amino Acids

85.42

Weight (kDa)

9.61

Isoelectric Point (pI)

44.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 28 - 276 4.1e-36 Reverse transcriptase (RNA-dependent DNA polymerase)
zf-RVT PF13966 549 - 635 2.2e-17 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000842)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15420 FvH4_2g00771 FvH4_3g29363
malus_domestica MD02G1293300.v1.1 MD06G1023000.v1.1 MD09G1232900.v1.1
prunus_persica Prupe.1G181500_v2.0.a1 Prupe.1G572100_v2.0.a1 Prupe.2G095500_v2.0.a1 Prupe.3G150400_v2.0.a1 Prupe.3G156200_v2.0.a1 Prupe.4G274400_v2.0.a1 Prupe.5G070700_v2.0.a1 Prupe.7G038000_v2.0.a1 Prupe.7G164000_v2.0.a1 Prupe.8G056500_v2.0.a1
pyrus_communis pycom04g02040 pycom05g04600 pycom07g05610 pycom08g16130 pycom11g16470 pycom12g07830 pycom15g38190 pycom215g00040
rosa_chinensis RchiOBHm_Chr1g0348881 RchiOBHm_Chr5g0032951 RchiOBHm_Chr6g0273271 RchiOBHm_Chr7g0218191 RchiOBHm_Chr7g0223721
rosa_multiflora Rmu_co8286615.1_g000001 Rmu_sc0001354.1_g000002 Rmu_sc0001925.1_g000003 Rmu_sc0001925.1_g000005 Rmu_sc0002221.1_g000004 Rmu_sc0002316.1_g000096 Rmu_sc0003291.1_g000005 Rmu_sc0003413.1_g000005 Rmu_sc0003542.1_g000011 Rmu_sc0004035.1_g000003 Rmu_sc0004035.1_g000004 Rmu_sc0004145.1_g000016 Rmu_sc0004660.1_g000004 Rmu_sc0005999.1_g000006 Rmu_sc0006123.1_g000002 Rmu_sc0006586.1_g000006 Rmu_sc0006889.1_g000029 Rmu_sc0008035.1_g000014 Rmu_sc0011962.1_g000002 Rmu_sc0029950.1_g000001 Rmu_sc0042409.1_g000001 Rmu_ssc0000238.1_g000013
rosa_roxburghii Rroxscaffold_2G00137410 Rroxscaffold_4G00312010 Rroxscaffold_4G00320800 Rroxscaffold_5G00341720 Rroxscaffold_5G00353530 Rroxscaffold_5G00360290 Rroxscaffold_7G00214170 Rroxscaffold_7G00215920
rosa_rugosa Rorug02G0157000
rosa_samantha Rh4BG196500 Rh6CG346400 Rh7BG241100 Rh7DG316300
rosa_wichuraiana Rw1G003610 Rw7G012170 Rw7G030150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 516
AccI GTMKAC 1 cut(s) 69
AciI CCGC 1 cut(s) 1002
AclWI GGATC 4 cut(s) 473, 857, 870, 1888
AcoI YGGCCR 5 cut(s) 577, 803, 1080, 1548, 1758
AcsI RAATTY 9 cut(s) 42, 213, 409, 446, 742, 854, 993, 1788, 1975
AcuI CTGAAG 1 cut(s) 1611
AdeI CACNNNGTG 1 cut(s) 1216
AfaI GTAC 1 cut(s) 345
AfiI CCNNNNNNNGG 3 cut(s) 188, 586, 833
AflII CTTAAG 1 cut(s) 287
AflIII ACRYGT 1 cut(s) 1778
AjnI CCWGG 2 cut(s) 954, 1617
AjuI GAANNNNNNNTTGG 4 cut(s) 146, 178, 1061, 1093
AleI CACNNNNGTG 1 cut(s) 1828
AluBI AGCT 9 cut(s) 402, 455, 719, 1244, 1467, 1625, 1963, 2138, 2207
AluI AGCT 9 cut(s) 402, 455, 719, 1244, 1467, 1625, 1963, 2138, 2207
Alw21I GWGCWC 1 cut(s) 1686
Alw26I GTCTC 5 cut(s) 181, 463, 700, 1206, 1590
Alw44I GTGCAC 1 cut(s) 1682
AlwI GGATC 4 cut(s) 473, 857, 870, 1888
AlwNI CAGNNNCTG 1 cut(s) 1204
Ama87I CYCGRG 1 cut(s) 1462
AoxI GGCC 8 cut(s) 92, 434, 577, 803, 839, 1080, 1548, 1758
ApaLI GTGCAC 1 cut(s) 1682
ApeKI GCWGC 4 cut(s) 37, 1335, 2135, 2207
ApoI RAATTY 9 cut(s) 42, 213, 409, 446, 742, 854, 993, 1788, 1975
AseI ATTAAT 1 cut(s) 1028
Asp700I GAANNNNTTC 3 cut(s) 911, 1260, 1575
AspS9I GGNCC 2 cut(s) 93, 1400
AsuHPI GGTGA 7 cut(s) 268, 480, 493, 1228, 1370, 1378, 1812
AvaI CYCGRG 1 cut(s) 1462
AvaII GGWCC 1 cut(s) 1400
BaeGI GKGCMC 1 cut(s) 1686
BaeI ACNNNNGTAYC 2 cut(s) 159, 192
BalI TGGCCA 3 cut(s) 1082, 1550, 1760
BamHI GGATCC 1 cut(s) 862
BarI GAAGNNNNNNTAC 2 cut(s) 330, 362
BbsI GAAGAC 3 cut(s) 648, 895, 2088
Bbv12I GWGCWC 1 cut(s) 1686
BbvI GCAGC 4 cut(s) 24, 1322, 2147, 2194
BccI CCATC 6 cut(s) 366, 1245, 1376, 1380, 1525, 1667
BciT130I CCWGG 2 cut(s) 956, 1619
BciVI GTATCC 1 cut(s) 160
BclI TGATCA 2 cut(s) 313, 423
BcoDI GTCTC 5 cut(s) 181, 463, 700, 1206, 1590
BfaI CTAG 1 cut(s) 1124
BfmI CTRYAG 2 cut(s) 88, 2208
BfrI CTTAAG 1 cut(s) 287
BfuAI ACCTGC 1 cut(s) 516
BfuI GTATCC 1 cut(s) 160
BisI GCNGC 4 cut(s) 38, 1336, 2136, 2208
BlsI GCNGC 4 cut(s) 39, 1337, 2137, 2209
BmcAI AGTACT 1 cut(s) 345
Bme1390I CCNGG 2 cut(s) 956, 1619
Bme18I GGWCC 1 cut(s) 1400
BmeT110I CYCGRG 1 cut(s) 1462
BmgT120I GGNCC 2 cut(s) 93, 1400
BmiI GGNNCC 4 cut(s) 864, 1401, 1616, 1725
BmrFI CCNGG 2 cut(s) 956, 1619
BmsI GCATC 3 cut(s) 211, 375, 1322
BpiI GAAGAC 3 cut(s) 648, 895, 2088
BplI GAGNNNNNCTC 4 cut(s) 1300, 1332, 1789, 1821
BpmI CTGGAG 1 cut(s) 2112
BpuEI CTTGAG 1 cut(s) 1159
BsaI GGTCTC 1 cut(s) 700
BsaJI CCNNGG 6 cut(s) 437, 829, 955, 1187, 1292, 1848
BsaXI ACNNNNNCTCC 4 cut(s) 163, 193, 1184, 1214
Bsc4I CCNNNNNNNGG 3 cut(s) 188, 586, 833
Bse1I ACTGG 4 cut(s) 115, 144, 866, 2095
Bse3DI GCAATG 2 cut(s) 231, 1879
BseBI CCWGG 2 cut(s) 956, 1619
BseDI CCNNGG 6 cut(s) 437, 829, 955, 1187, 1292, 1848
BseGI GGATG 7 cut(s) 226, 390, 398, 1256, 1387, 1391, 2203
BseLI CCNNNNNNNGG 3 cut(s) 188, 586, 833
BseMI GCAATG 2 cut(s) 231, 1879
BseMII CTCAG 2 cut(s) 512, 711
BseNI ACTGG 4 cut(s) 115, 144, 866, 2095
BseRI GAGGAG 1 cut(s) 1868
BseSI GKGCMC 1 cut(s) 1686
BseXI GCAGC 4 cut(s) 24, 1322, 2147, 2194
BseYI CCCAGC 1 cut(s) 734
BsgI GTGCAG 1 cut(s) 1321
BshFI GGCC 8 cut(s) 94, 436, 579, 805, 841, 1082, 1550, 1760
BsiHKAI GWGCWC 1 cut(s) 1686
BsiHKCI CYCGRG 1 cut(s) 1462
BslFI GGGAC 1 cut(s) 1413
BslI CCNNNNNNNGG 3 cut(s) 188, 586, 833
BsmAI GTCTC 5 cut(s) 181, 463, 700, 1206, 1590
BsmFI GGGAC 1 cut(s) 1413
BsnI GGCC 8 cut(s) 94, 436, 579, 805, 841, 1082, 1550, 1760
Bso31I GGTCTC 1 cut(s) 700
BsoBI CYCGRG 1 cut(s) 1462
Bsp1286I GDGCHC 1 cut(s) 1686
Bsp143I GATC 7 cut(s) 291, 313, 423, 478, 553, 862, 1893
Bsp19I CCATGG 1 cut(s) 1292
BspACI CCGC 1 cut(s) 1002
BspANI GGCC 8 cut(s) 94, 436, 579, 805, 841, 1082, 1550, 1760
BspCNI CTCAG 2 cut(s) 513, 710
BspLI GGNNCC 4 cut(s) 864, 1401, 1616, 1725
BspMAI CTGCAG 1 cut(s) 2212
BspMI ACCTGC 1 cut(s) 516
BspPI GGATC 4 cut(s) 473, 857, 870, 1888
BspTI CTTAAG 1 cut(s) 287
BspTNI GGTCTC 1 cut(s) 700
BsrDI GCAATG 2 cut(s) 231, 1879
BsrI ACTGG 4 cut(s) 115, 144, 866, 2095
BssECI CCNNGG 6 cut(s) 437, 829, 955, 1187, 1292, 1848
BssMI GATC 7 cut(s) 291, 313, 423, 478, 553, 862, 1893
BssT1I CCWWGG 4 cut(s) 437, 1187, 1292, 1848
Bst2UI CCWGG 2 cut(s) 956, 1619
Bst4CI ACNGT 7 cut(s) 175, 486, 1200, 1323, 1358, 1404, 1902
Bst6I CTCTTC 2 cut(s) 246, 1566
BstAFI CTTAAG 1 cut(s) 287
BstC8I GCNNGC 3 cut(s) 771, 803, 965
BstDEI CTNAG 7 cut(s) 505, 521, 697, 720, 1167, 1328, 1967
BstDSI CCRYGG 1 cut(s) 1292
BstF5I GGATG 7 cut(s) 226, 390, 398, 1256, 1387, 1391, 2203
BstKTI GATC 7 cut(s) 294, 316, 426, 481, 556, 865, 1896
BstMAI GTCTC 5 cut(s) 181, 463, 700, 1206, 1590
BstMBI GATC 7 cut(s) 291, 313, 423, 478, 553, 862, 1893
BstMWI GCNNNNNNNGC 2 cut(s) 1163, 2144
BstNI CCWGG 2 cut(s) 956, 1619
BstNSI RCATGY 2 cut(s) 967, 1782
BstSCI CCNGG 2 cut(s) 954, 1617
BstSFI CTRYAG 2 cut(s) 88, 2208
BstSLI GKGCMC 1 cut(s) 1686
BstV1I GCAGC 4 cut(s) 24, 1322, 2147, 2194
BstV2I GAAGAC 3 cut(s) 648, 895, 2088
BstX2I RGATCY 1 cut(s) 862
BstXI CCANNNNNNTGG 2 cut(s) 139, 1710
BstYI RGATCY 1 cut(s) 862
BsuI GTATCC 1 cut(s) 160
BsuRI GGCC 8 cut(s) 94, 436, 579, 805, 841, 1082, 1550, 1760
BtgI CCRYGG 1 cut(s) 1292
BtsCI GGATG 7 cut(s) 226, 390, 398, 1256, 1387, 1391, 2203
BtsIMutI CAGTG 4 cut(s) 137, 171, 482, 873
BveI ACCTGC 1 cut(s) 516
Cac8I GCNNGC 3 cut(s) 771, 803, 965
CaiI CAGNNNCTG 1 cut(s) 1204
Cfr13I GGNCC 2 cut(s) 93, 1400
Csp6I GTAC 1 cut(s) 344
CspCI CAANNNNNGTGG 4 cut(s) 225, 260, 1886, 1921
CviAII CATG 9 cut(s) 19, 317, 964, 999, 1054, 1268, 1293, 1641, 1779
CviQI GTAC 1 cut(s) 344
DdeI CTNAG 7 cut(s) 505, 521, 697, 720, 1167, 1328, 1967
DpnI GATC 7 cut(s) 293, 315, 425, 480, 555, 864, 1895
DpnII GATC 7 cut(s) 291, 313, 423, 478, 553, 862, 1893
DraIII CACNNNGTG 1 cut(s) 1216
EaeI YGGCCR 5 cut(s) 577, 803, 1080, 1548, 1758
Eam1104I CTCTTC 2 cut(s) 246, 1566
EarI CTCTTC 2 cut(s) 246, 1566
Eco130I CCWWGG 4 cut(s) 437, 1187, 1292, 1848
Eco31I GGTCTC 1 cut(s) 700
Eco32I GATATC 2 cut(s) 597, 1603
Eco47I GGWCC 1 cut(s) 1400
Eco57I CTGAAG 1 cut(s) 1611
Eco88I CYCGRG 1 cut(s) 1462
EcoRI GAATTC 1 cut(s) 1788
EcoRII CCWGG 2 cut(s) 954, 1617
EcoRV GATATC 2 cut(s) 597, 1603
EcoT14I CCWWGG 4 cut(s) 437, 1187, 1292, 1848
ErhI CCWWGG 4 cut(s) 437, 1187, 1292, 1848
FaeI CATG 9 cut(s) 22, 320, 967, 1002, 1057, 1271, 1296, 1644, 1782
FalI AAGNNNNNCTT 2 cut(s) 281, 313
FaqI GGGAC 1 cut(s) 1413
FatI CATG 9 cut(s) 18, 316, 963, 998, 1053, 1267, 1292, 1640, 1778
FauI CCCGC 1 cut(s) 995
FbaI TGATCA 2 cut(s) 313, 423
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 4 cut(s) 38, 1336, 2136, 2208
FokI GGATG 7 cut(s) 233, 397, 405, 1263, 1394, 1398, 2210
Fsp4HI GCNGC 4 cut(s) 38, 1336, 2136, 2208
FspBI CTAG 1 cut(s) 1124
GluI GCNGC 4 cut(s) 38, 1336, 2136, 2208
GsaI CCCAGC 1 cut(s) 738
GsuI CTGGAG 1 cut(s) 2112
HaeIII GGCC 8 cut(s) 94, 436, 579, 805, 841, 1082, 1550, 1760
Hin1II CATG 9 cut(s) 22, 320, 967, 1002, 1057, 1271, 1296, 1644, 1782
HindIII AAGCTT 2 cut(s) 453, 1623
HinfI GANTC 5 cut(s) 184, 1144, 1182, 1193, 1768
HphI GGTGA 7 cut(s) 268, 480, 493, 1228, 1370, 1378, 1812
Hpy166II GTNNAC 3 cut(s) 6, 70, 1684
Hpy188I TCNGA 7 cut(s) 183, 522, 748, 833, 1149, 1607, 1731
Hpy188III TCNNGA 9 cut(s) 147, 335, 459, 634, 699, 921, 1462, 1919, 2044
Hpy8I GTNNAC 3 cut(s) 6, 70, 1684
Hpy99I CGWCG 1 cut(s) 620
HpyCH4III ACNGT 7 cut(s) 175, 486, 1200, 1323, 1358, 1404, 1902
HpyCH4IV ACGT 3 cut(s) 1015, 1340, 2062
HpyCH4V TGCA 9 cut(s) 40, 236, 388, 407, 1338, 1644, 1684, 1842, 2210
HpyF10VI GCNNNNNNNGC 2 cut(s) 1163, 2144
HpyF3I CTNAG 7 cut(s) 505, 521, 697, 720, 1167, 1328, 1967
HpySE526I ACGT 3 cut(s) 1015, 1340, 2062
Hsp92II CATG 9 cut(s) 22, 320, 967, 1002, 1057, 1271, 1296, 1644, 1782
Ksp22I TGATCA 2 cut(s) 313, 423
Kzo9I GATC 7 cut(s) 291, 313, 423, 478, 553, 862, 1893
LmnI GCTCC 3 cut(s) 1411, 1665, 1889
Lsp1109I GCAGC 4 cut(s) 24, 1322, 2147, 2194
LweI GCATC 3 cut(s) 211, 375, 1322
MaeI CTAG 1 cut(s) 1124
MaeII ACGT 3 cut(s) 1015, 1340, 2062
MaeIII GTNAC 9 cut(s) 79, 106, 486, 1096, 1194, 1390, 1781, 2063, 2152
MalI GATC 7 cut(s) 293, 315, 425, 480, 555, 864, 1895
MboI GATC 7 cut(s) 291, 313, 423, 478, 553, 862, 1893
MflI RGATCY 1 cut(s) 862
MhlI GDGCHC 1 cut(s) 1686
MlsI TGGCCA 3 cut(s) 1082, 1550, 1760
MluNI TGGCCA 3 cut(s) 1082, 1550, 1760
MlyI GAGTC 1 cut(s) 1202
MmeI TCCRAC 3 cut(s) 881, 1478, 1921
Mox20I TGGCCA 3 cut(s) 1082, 1550, 1760
MroXI GAANNNNTTC 3 cut(s) 911, 1260, 1575
MscI TGGCCA 3 cut(s) 1082, 1550, 1760
MslI CAYNNNNRTG 1 cut(s) 1828
Msp20I TGGCCA 3 cut(s) 1082, 1550, 1760
MspCI CTTAAG 1 cut(s) 287
MspR9I CCNGG 2 cut(s) 956, 1619
MvaI CCWGG 2 cut(s) 956, 1619
MwoI GCNNNNNNNGC 2 cut(s) 1163, 2144
NcoI CCATGG 1 cut(s) 1292
NdeII GATC 7 cut(s) 291, 313, 423, 478, 553, 862, 1893
NlaIII CATG 9 cut(s) 22, 320, 967, 1002, 1057, 1271, 1296, 1644, 1782
NlaIV GGNNCC 4 cut(s) 864, 1401, 1616, 1725
NmuCI GTSAC 5 cut(s) 79, 486, 1096, 1194, 1781
NspI RCATGY 2 cut(s) 967, 1782
OliI CACNNNNGTG 1 cut(s) 1828
PaeI GCATGC 1 cut(s) 967
PaeR7I CTCGAG 1 cut(s) 1462
PciI ACATGT 1 cut(s) 1778
PdmI GAANNNNTTC 3 cut(s) 911, 1260, 1575
PfeI GAWTC 4 cut(s) 184, 1144, 1182, 1768
PkrI GCNGC 4 cut(s) 39, 1337, 2137, 2209
PleI GAGTC 1 cut(s) 1201
PpsI GAGTC 1 cut(s) 1201
PscI ACATGT 1 cut(s) 1778
PshBI ATTAAT 1 cut(s) 1028
Psp6I CCWGG 2 cut(s) 954, 1617
PspFI CCCAGC 1 cut(s) 734
PspGI CCWGG 2 cut(s) 954, 1617
PspN4I GGNNCC 4 cut(s) 864, 1401, 1616, 1725
PspPI GGNCC 2 cut(s) 93, 1400
PsrI GAACNNNNNNTAC 2 cut(s) 501, 533
PstI CTGCAG 1 cut(s) 2212
PstNI CAGNNNCTG 1 cut(s) 1204
PsuI RGATCY 1 cut(s) 862
RsaI GTAC 1 cut(s) 345
RsaNI GTAC 1 cut(s) 344
RseI CAYNNNNRTG 1 cut(s) 1828
SatI GCNGC 4 cut(s) 38, 1336, 2136, 2208
Sau3AI GATC 7 cut(s) 291, 313, 423, 478, 553, 862, 1893
Sau96I GGNCC 2 cut(s) 93, 1400
ScaI AGTACT 1 cut(s) 345
SchI GAGTC 1 cut(s) 1202
ScrFI CCNGG 2 cut(s) 956, 1619
SduI GDGCHC 1 cut(s) 1686
SfaNI GCATC 3 cut(s) 211, 375, 1322
SfcI CTRYAG 2 cut(s) 88, 2208
Sfr274I CTCGAG 1 cut(s) 1462
SinI GGWCC 1 cut(s) 1400
SlaI CTCGAG 1 cut(s) 1462
SmiMI CAYNNNNRTG 1 cut(s) 1828
SmlI CTYRAG 3 cut(s) 287, 1138, 1462
SmoI CTYRAG 3 cut(s) 287, 1138, 1462
SphI GCATGC 1 cut(s) 967
SsiI CCGC 1 cut(s) 1002
SspI AATATT 1 cut(s) 2129
SspMI CTAG 1 cut(s) 1124
StyD4I CCNGG 2 cut(s) 954, 1617
StyI CCWWGG 4 cut(s) 437, 1187, 1292, 1848
TaaI ACNGT 7 cut(s) 175, 486, 1200, 1323, 1358, 1404, 1902
TaiI ACGT 3 cut(s) 1018, 1343, 2065
TaqI TCGA 9 cut(s) 294, 513, 1285, 1307, 1439, 1463, 1579, 1892, 1959
TaqII GACCGA 1 cut(s) 682
TatI WGTACW 1 cut(s) 343
TfiI GAWTC 4 cut(s) 184, 1144, 1182, 1768
TscAI CASTG 4 cut(s) 144, 178, 489, 873
TseFI GTSAC 5 cut(s) 79, 486, 1096, 1194, 1781
TseI GCWGC 4 cut(s) 37, 1335, 2135, 2207
Tsp45I GTSAC 5 cut(s) 79, 486, 1096, 1194, 1781
TspDTI ATGAA 5 cut(s) 4, 255, 1046, 1647, 1868
TspGWI ACGGA 3 cut(s) 98, 406, 1678
TspRI CASTG 4 cut(s) 144, 178, 489, 873
Vha464I CTTAAG 1 cut(s) 287
VneI GTGCAC 1 cut(s) 1682
VpaK11BI GGWCC 1 cut(s) 1400
VspI ATTAAT 1 cut(s) 1028
XapI RAATTY 9 cut(s) 42, 213, 409, 446, 742, 854, 993, 1788, 1975
XceI RCATGY 2 cut(s) 967, 1782
XhoI CTCGAG 1 cut(s) 1462
XmiI GTMKAC 1 cut(s) 69
XmnI GAANNNNTTC 3 cut(s) 911, 1260, 1575
XspI CTAG 1 cut(s) 1124
ZrmI AGTACT 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.