RchiOBHm_Chr7g0223721

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000842
Physical Location & Seq
Forward (+)
45592216 .. 45593227
1012 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20031

Sequence Viewer

Length: 648 bp
ATGAATCCTAATAAGGCTCCGGGCCCAGATGGTTTTAACTGTTGCTTCTTTCAAAAGGCTTGGTCGATTATTGGTGAAGATGTTGTTGCTGCTGTTAAGGAATTTTTCTCTTCTGGTCTCCTCTTAAAGGAGTTAAATTCCACCATCATTACTCTTGTTCCTAAAGTGGCTAACCCCACTACTATGAGTGATTTCAGACCTATATCCTGCTGTAACACCTTATATAAAATTATTGCCAAGTTGTTGGCGAACAAGCTAAAGGGCGTTCTTCATCTTATTGTTGGGCCTTCCCAATCTGCCTTCATTCCTGGGCGCAGAATTGGAGACAATATCCTTCTTGCTCAAGAACTCCTTCGTGATTACCATAAAGCTATTGGTCATCCTAGATGCACTCTTATGGTGGACATTATGAAAGCTTATGACACTTTTGAATGGGATTTCATTCTTGCGACCCTTGAAGCTTTTAATATTCCTCCTACTTTGATTAGTTGGATCAAGAGCTGCATTTCTTCCCTGAGATTTTCTGTGGCTGTTAATGGTGAACTGGCAGGTTTCTTTGCTAGCAAGTGGGGTTTAAGACAAGGCGACCCCTTGTCTCCCTACCTTTTTGTTATTGCTATGGAAGCCCTCTCTCTTTGTATTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04130 map04130
Pfam Domains
Protein Families
DUF4283 Exo_endo_phos RVT_1 zf-RVT

Protein Analysis

215

Amino Acids

23.74

Weight (kDa)

8.46

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 60 - 210 1.1e-25 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000842)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15420 FvH4_2g00771 FvH4_3g29363
malus_domestica MD02G1293300.v1.1 MD06G1023000.v1.1 MD09G1232900.v1.1
prunus_persica Prupe.1G181500_v2.0.a1 Prupe.1G572100_v2.0.a1 Prupe.2G095500_v2.0.a1 Prupe.3G150400_v2.0.a1 Prupe.3G156200_v2.0.a1 Prupe.4G274400_v2.0.a1 Prupe.5G070700_v2.0.a1 Prupe.7G038000_v2.0.a1 Prupe.7G164000_v2.0.a1 Prupe.8G056500_v2.0.a1
pyrus_communis pycom04g02040 pycom05g04600 pycom07g05610 pycom08g16130 pycom11g16470 pycom12g07830 pycom15g38190 pycom215g00040
rosa_chinensis RchiOBHm_Chr1g0348881 RchiOBHm_Chr5g0032951 RchiOBHm_Chr6g0273271 RchiOBHm_Chr7g0218191 RchiOBHm_Chr7g0223721
rosa_multiflora Rmu_co8286615.1_g000001 Rmu_sc0001354.1_g000002 Rmu_sc0001925.1_g000003 Rmu_sc0001925.1_g000005 Rmu_sc0002221.1_g000004 Rmu_sc0002316.1_g000096 Rmu_sc0003291.1_g000005 Rmu_sc0003413.1_g000005 Rmu_sc0003542.1_g000011 Rmu_sc0004035.1_g000003 Rmu_sc0004035.1_g000004 Rmu_sc0004145.1_g000016 Rmu_sc0004660.1_g000004 Rmu_sc0005999.1_g000006 Rmu_sc0006123.1_g000002 Rmu_sc0006586.1_g000006 Rmu_sc0006889.1_g000029 Rmu_sc0008035.1_g000014 Rmu_sc0011962.1_g000002 Rmu_sc0029950.1_g000001 Rmu_sc0042409.1_g000001 Rmu_ssc0000238.1_g000013
rosa_roxburghii Rroxscaffold_2G00137410 Rroxscaffold_4G00312010 Rroxscaffold_4G00320800 Rroxscaffold_5G00341720 Rroxscaffold_5G00353530 Rroxscaffold_5G00360290 Rroxscaffold_7G00214170 Rroxscaffold_7G00215920
rosa_rugosa Rorug02G0157000
rosa_samantha Rh4BG196500 Rh6CG346400 Rh7BG241100 Rh7DG316300
rosa_wichuraiana Rw1G003610 Rw7G012170 Rw7G030150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 539
AclWI GGATC 1 cut(s) 500
AcsI RAATTY 2 cut(s) 101, 136
AfiI CCNNNNNNNGG 1 cut(s) 127
AgsI TTSAA 3 cut(s) 53, 431, 458
AhdI GACNNNNNGTC 1 cut(s) 592
AjnI CCWGG 1 cut(s) 307
AluBI AGCT 5 cut(s) 256, 371, 416, 461, 501
AluI AGCT 5 cut(s) 256, 371, 416, 461, 501
Alw26I GTCTC 3 cut(s) 122, 318, 600
AlwI GGATC 1 cut(s) 500
AoxI GGCC 2 cut(s) 22, 284
ApaI GGGCCC 1 cut(s) 26
ApeKI GCWGC 2 cut(s) 89, 501
ApoI RAATTY 2 cut(s) 101, 136
Asp700I GAANNNNTTC 1 cut(s) 351
AspLEI GCGC 1 cut(s) 315
AspS9I GGNCC 3 cut(s) 22, 23, 284
AsuC2I CCSGG 1 cut(s) 21
AsuHPI GGTGA 2 cut(s) 86, 551
AsuNHI GCTAGC 1 cut(s) 560
BaeGI GKGCMC 1 cut(s) 26
BanII GRGCYC 1 cut(s) 26
BbvI GCAGC 2 cut(s) 76, 488
BccI CCATC 2 cut(s) 23, 152
BciT130I CCWGG 1 cut(s) 309
BcnI CCSGG 1 cut(s) 21
BcoDI GTCTC 3 cut(s) 122, 318, 600
BfaI CTAG 2 cut(s) 384, 561
BfuAI ACCTGC 1 cut(s) 539
BisI GCNGC 2 cut(s) 90, 502
BlsI GCNGC 2 cut(s) 91, 503
Bme1390I CCNGG 2 cut(s) 21, 309
BmeRI GACNNNNNGTC 1 cut(s) 592
BmgT120I GGNCC 3 cut(s) 22, 23, 284
BmiI GGNNCC 2 cut(s) 18, 24
BmrFI CCNGG 2 cut(s) 21, 309
BmsI GCATC 1 cut(s) 377
BmtI GCTAGC 1 cut(s) 564
BpuEI CTTGAG 1 cut(s) 327
BpuMI CCSGG 1 cut(s) 21
BsaI GGTCTC 1 cut(s) 122
BsaJI CCNNGG 1 cut(s) 308
Bsc4I CCNNNNNNNGG 1 cut(s) 127
Bse1I ACTGG 1 cut(s) 549
BseBI CCWGG 1 cut(s) 309
BseDI CCNNGG 1 cut(s) 308
BseGI GGATG 1 cut(s) 379
BseLI CCNNNNNNNGG 1 cut(s) 127
BseMII CTCAG 1 cut(s) 506
BseNI ACTGG 1 cut(s) 549
BseRI GAGGAG 1 cut(s) 110
BseSI GKGCMC 1 cut(s) 26
BseXI GCAGC 2 cut(s) 76, 488
BshFI GGCC 2 cut(s) 24, 286
BsiSI CCGG 1 cut(s) 20
BslI CCNNNNNNNGG 1 cut(s) 127
BsmAI GTCTC 3 cut(s) 122, 318, 600
BsnI GGCC 2 cut(s) 24, 286
Bso31I GGTCTC 1 cut(s) 122
Bsp120I GGGCCC 1 cut(s) 22
Bsp1286I GDGCHC 1 cut(s) 26
Bsp143I GATC 1 cut(s) 492
BspANI GGCC 2 cut(s) 24, 286
BspCNI CTCAG 1 cut(s) 507
BspLI GGNNCC 2 cut(s) 18, 24
BspMI ACCTGC 1 cut(s) 539
BspOI GCTAGC 1 cut(s) 564
BspPI GGATC 1 cut(s) 500
BspTNI GGTCTC 1 cut(s) 122
BsrI ACTGG 1 cut(s) 549
BssECI CCNNGG 1 cut(s) 308
BssMI GATC 1 cut(s) 492
Bst2UI CCWGG 1 cut(s) 309
Bst4CI ACNGT 1 cut(s) 41
Bst6I CTCTTC 1 cut(s) 115
BstC8I GCNNGC 1 cut(s) 562
BstDEI CTNAG 1 cut(s) 515
BstENI CCTNNNNNAGG 1 cut(s) 125
BstF5I GGATG 1 cut(s) 379
BstHHI GCGC 1 cut(s) 315
BstKTI GATC 1 cut(s) 495
BstMAI GTCTC 3 cut(s) 122, 318, 600
BstMBI GATC 1 cut(s) 492
BstMWI GCNNNNNNNGC 1 cut(s) 623
BstNI CCWGG 1 cut(s) 309
BstSCI CCNGG 2 cut(s) 19, 307
BstSLI GKGCMC 1 cut(s) 26
BstV1I GCAGC 2 cut(s) 76, 488
BstXI CCANNNNNNTGG 1 cut(s) 244
BsuRI GGCC 2 cut(s) 24, 286
BtsCI GGATG 1 cut(s) 379
BveI ACCTGC 1 cut(s) 539
Cac8I GCNNGC 1 cut(s) 562
CfoI GCGC 1 cut(s) 315
Cfr13I GGNCC 3 cut(s) 22, 23, 284
DdeI CTNAG 1 cut(s) 515
DpnI GATC 1 cut(s) 494
DpnII GATC 1 cut(s) 492
DriI GACNNNNNGTC 1 cut(s) 592
Eam1104I CTCTTC 1 cut(s) 115
Eam1105I GACNNNNNGTC 1 cut(s) 592
EarI CTCTTC 1 cut(s) 115
Eco24I GRGCYC 1 cut(s) 26
Eco31I GGTCTC 1 cut(s) 122
EcoNI CCTNNNNNAGG 1 cut(s) 125
EcoRII CCWGG 1 cut(s) 307
EcoT38I GRGCYC 1 cut(s) 26
FaiI YATR 9 cut(s) 185, 203, 223, 225, 366, 398, 410, 420, 620
FalI AAGNNNNNCTT 2 cut(s) 336, 368
Fnu4HI GCNGC 2 cut(s) 90, 502
FokI GGATG 1 cut(s) 366
FriOI GRGCYC 1 cut(s) 26
Fsp4HI GCNGC 2 cut(s) 90, 502
FspBI CTAG 2 cut(s) 384, 561
GlaI GCGC 1 cut(s) 314
GluI GCNGC 2 cut(s) 90, 502
HaeIII GGCC 2 cut(s) 24, 286
HapII CCGG 1 cut(s) 20
HhaI GCGC 1 cut(s) 315
Hin6I GCGC 1 cut(s) 313
HinP1I GCGC 1 cut(s) 313
HindIII AAGCTT 2 cut(s) 414, 459
HinfI GANTC 1 cut(s) 4
HpaII CCGG 1 cut(s) 20
HphI GGTGA 2 cut(s) 86, 551
Hpy166II GTNNAC 2 cut(s) 403, 542
Hpy188I TCNGA 1 cut(s) 197
Hpy188III TCNNGA 3 cut(s) 344, 356, 496
Hpy8I GTNNAC 2 cut(s) 403, 542
HpyAV CCTTC 4 cut(s) 297, 310, 344, 362
HpyCH4III ACNGT 1 cut(s) 41
HpyCH4V TGCA 2 cut(s) 390, 504
HpyF10VI GCNNNNNNNGC 1 cut(s) 623
HpyF3I CTNAG 1 cut(s) 515
HspAI GCGC 1 cut(s) 313
Kzo9I GATC 1 cut(s) 492
LmnI GCTCC 1 cut(s) 22
LpnPI CCDG 9 cut(s) 33, 39, 99, 220, 294, 321, 527, 530, 534
Lsp1109I GCAGC 2 cut(s) 76, 488
LweI GCATC 1 cut(s) 377
MaeI CTAG 2 cut(s) 384, 561
MaeIII GTNAC 1 cut(s) 212
MalI GATC 1 cut(s) 494
MboI GATC 1 cut(s) 492
MboII GAAGA 4 cut(s) 89, 102, 260, 501
MhlI GDGCHC 1 cut(s) 26
MluCI AATT 4 cut(s) 101, 136, 228, 318
MmeI TCCRAC 1 cut(s) 470
MnlI CCTC 3 cut(s) 131, 483, 638
MroXI GAANNNNTTC 1 cut(s) 351
MseI TTAA 7 cut(s) 36, 96, 125, 134, 465, 534, 575
MslI CAYNNNNRTG 2 cut(s) 182, 395
MspI CCGG 1 cut(s) 20
MspR9I CCNGG 2 cut(s) 21, 309
MvaI CCWGG 1 cut(s) 309
MwoI GCNNNNNNNGC 1 cut(s) 623
NciI CCSGG 1 cut(s) 21
NdeII GATC 1 cut(s) 492
NheI GCTAGC 1 cut(s) 560
NlaIV GGNNCC 2 cut(s) 18, 24
PdmI GAANNNNTTC 1 cut(s) 351
PfeI GAWTC 1 cut(s) 4
PkrI GCNGC 2 cut(s) 91, 503
Psp6I CCWGG 1 cut(s) 307
PspGI CCWGG 1 cut(s) 307
PspN4I GGNNCC 2 cut(s) 18, 24
PspOMI GGGCCC 1 cut(s) 22
PspPI GGNCC 3 cut(s) 22, 23, 284
RseI CAYNNNNRTG 2 cut(s) 182, 395
SaqAI TTAA 7 cut(s) 36, 96, 125, 134, 465, 534, 575
SatI GCNGC 2 cut(s) 90, 502
Sau3AI GATC 1 cut(s) 492
Sau96I GGNCC 3 cut(s) 22, 23, 284
ScrFI CCNGG 2 cut(s) 21, 309
SduI GDGCHC 1 cut(s) 26
SetI ASST 9 cut(s) 202, 221, 258, 373, 418, 463, 503, 553, 606
SfaNI GCATC 1 cut(s) 377
SmiMI CAYNNNNRTG 2 cut(s) 182, 395
SmlI CTYRAG 1 cut(s) 342
SmoI CTYRAG 1 cut(s) 342
Sse9I AATT 4 cut(s) 101, 136, 228, 318
SspI AATATT 1 cut(s) 469
SspMI CTAG 2 cut(s) 384, 561
StyD4I CCNGG 2 cut(s) 19, 307
TaaI ACNGT 1 cut(s) 41
TaqI TCGA 1 cut(s) 65
TasI AATT 4 cut(s) 101, 136, 228, 318
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 7 cut(s) 36, 96, 125, 134, 465, 534, 575
Tru9I TTAA 7 cut(s) 36, 96, 125, 134, 465, 534, 575
TseI GCWGC 2 cut(s) 89, 501
TspDTI ATGAA 5 cut(s) 17, 260, 292, 425, 430
XagI CCTNNNNNAGG 1 cut(s) 125
XapI RAATTY 2 cut(s) 101, 136
XcmI CCANNNNNNNNNTGG 1 cut(s) 371
XmnI GAANNNNTTC 1 cut(s) 351
XspI CTAG 2 cut(s) 384, 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.