FvH4_3g29363

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000842
Physical Location & Seq
Reverse (-)
22469806 .. 22470878
1073 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g29363.t1

Sequence Viewer

Length: 825 bp
ATGAAGGAAGCACCACAGGATTTGAATTTTACTTATGTGGTTCTTATTCCTAAAGTGAAAGAGGTCCAGAGTATGACTCAATTGCATCCGATTGCTCTATGCAATGTTATTTATAAGATTGCTTCCAAGGTCTTAGCTAACAGATTGAAGTCCTTCCTTCCAACGATTATTTCTCCGAAACAAAGTGTTTTTGTCCCTGATAGACTGATTTCTGATAACACCTTAGTGGCTTCAGAGCTAGCTTACTATATGCATAAATTGCGTAGAGGACAAGTAGGTTTCATGGCGCTCAAGTTGGATATTAGTAAGGCATATGATCGGTTGGAACGAGATTTTTTGAAGAAAATCATGTTGAGGATGAGATTTGCTCCTACTTGGGTGGACCAGATTATGCATTGTCTCTCTATTGCCAGGACTGAGACAGGTGATCCCATCTCACCTTATTTGTTTATGGTATGTGCTGAAGGATTATCGGCTTTGATTTCTCAGTCTGTTGCTCAGGGTAATTGGCGTGGTTTGCAAGTCTGTGATGGGGCTTCCATGATCAGTCATTTACTTTTTGCGGATGATATTATGTTGTATGCTAATGCTTTGCCACGGTTTTTGCATCATAGTTGCGGGCTAGGTTTGGATTGGATTTGGTTGCCGGATCAAAGTGATGTCAAGTGGTTATTGGTGGTCGGCGGGCCGGTTTCGATGTCGAGCACCAGTTGGTTGACTGATGTTGGTGATCAAATGTTGGTGTTAGATCTTGAGGAGCGCCGAGGGGTAGGCGACACGTTTTTTACTTCATCAAATGGTAACTGCTGTTTAGTGGCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

275

Amino Acids

30.75

Weight (kDa)

6.59

Isoelectric Point (pI)

41.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 17 - 198 2.7e-19 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000842)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15420 FvH4_2g00771 FvH4_3g29363
malus_domestica MD02G1293300.v1.1 MD06G1023000.v1.1 MD09G1232900.v1.1
prunus_persica Prupe.1G181500_v2.0.a1 Prupe.1G572100_v2.0.a1 Prupe.2G095500_v2.0.a1 Prupe.3G150400_v2.0.a1 Prupe.3G156200_v2.0.a1 Prupe.4G274400_v2.0.a1 Prupe.5G070700_v2.0.a1 Prupe.7G038000_v2.0.a1 Prupe.7G164000_v2.0.a1 Prupe.8G056500_v2.0.a1
pyrus_communis pycom04g02040 pycom05g04600 pycom07g05610 pycom08g16130 pycom11g16470 pycom12g07830 pycom15g38190 pycom215g00040
rosa_chinensis RchiOBHm_Chr1g0348881 RchiOBHm_Chr5g0032951 RchiOBHm_Chr6g0273271 RchiOBHm_Chr7g0218191 RchiOBHm_Chr7g0223721
rosa_multiflora Rmu_co8286615.1_g000001 Rmu_sc0001354.1_g000002 Rmu_sc0001925.1_g000003 Rmu_sc0001925.1_g000005 Rmu_sc0002221.1_g000004 Rmu_sc0002316.1_g000096 Rmu_sc0003291.1_g000005 Rmu_sc0003413.1_g000005 Rmu_sc0003542.1_g000011 Rmu_sc0004035.1_g000003 Rmu_sc0004035.1_g000004 Rmu_sc0004145.1_g000016 Rmu_sc0004660.1_g000004 Rmu_sc0005999.1_g000006 Rmu_sc0006123.1_g000002 Rmu_sc0006586.1_g000006 Rmu_sc0006889.1_g000029 Rmu_sc0008035.1_g000014 Rmu_sc0011962.1_g000002 Rmu_sc0029950.1_g000001 Rmu_sc0042409.1_g000001 Rmu_ssc0000238.1_g000013
rosa_roxburghii Rroxscaffold_2G00137410 Rroxscaffold_4G00312010 Rroxscaffold_4G00320800 Rroxscaffold_5G00341720 Rroxscaffold_5G00353530 Rroxscaffold_5G00360290 Rroxscaffold_7G00214170 Rroxscaffold_7G00215920
rosa_rugosa Rorug02G0157000
rosa_samantha Rh4BG196500 Rh6CG346400 Rh7BG241100 Rh7DG316300
rosa_wichuraiana Rw1G003610 Rw7G012170 Rw7G030150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 114
AciI CCGC 3 cut(s) 563, 618, 684
AclWI GGATC 2 cut(s) 422, 657
AcsI RAATTY 1 cut(s) 25
AcuI CTGAAG 2 cut(s) 216, 483
AflIII ACRYGT 1 cut(s) 777
AgsI TTSAA 3 cut(s) 25, 148, 340
AjnI CCWGG 1 cut(s) 410
AjuI GAANNNNNNNTTGG 2 cut(s) 154, 186
AleI CACNNNNGTG 1 cut(s) 224
AluBI AGCT 3 cut(s) 137, 238, 242
AluI AGCT 3 cut(s) 137, 238, 242
Alw21I GWGCWC 1 cut(s) 707
Alw26I GTCTC 2 cut(s) 404, 413
AlwI GGATC 2 cut(s) 422, 657
AoxI GGCC 1 cut(s) 686
ApoI RAATTY 1 cut(s) 25
Asp700I GAANNNNTTC 1 cut(s) 152
AspLEI GCGC 2 cut(s) 289, 762
AspS9I GGNCC 3 cut(s) 64, 382, 686
AsuHPI GGTGA 3 cut(s) 429, 437, 740
AsuNHI GCTAGC 1 cut(s) 238
AvaII GGWCC 2 cut(s) 64, 382
Bbv12I GWGCWC 1 cut(s) 707
BccI CCATC 2 cut(s) 440, 524
BciT130I CCWGG 1 cut(s) 412
BclI TGATCA 2 cut(s) 543, 730
BcoDI GTCTC 2 cut(s) 404, 413
BfaI CTAG 2 cut(s) 239, 623
BfoI RGCGCY 2 cut(s) 290, 763
BglII AGATCT 1 cut(s) 748
Bme1390I CCNGG 1 cut(s) 412
Bme18I GGWCC 2 cut(s) 64, 382
BmgT120I GGNCC 3 cut(s) 64, 382, 686
BmrFI CCNGG 1 cut(s) 412
BmsI GCATC 2 cut(s) 94, 616
BmtI GCTAGC 1 cut(s) 242
BplI GAGNNNNNCTC 4 cut(s) 61, 93, 352, 384
Bpu10I CCTNAGC 1 cut(s) 498
BpuEI CTTGAG 2 cut(s) 275, 773
BsaJI CCNNGG 3 cut(s) 126, 596, 763
Bse118I RCCGGY 1 cut(s) 688
Bse1I ACTGG 1 cut(s) 708
Bse3DI GCAATG 1 cut(s) 109
BseBI CCWGG 1 cut(s) 412
BseDI CCNNGG 3 cut(s) 126, 596, 763
BseGI GGATG 3 cut(s) 85, 363, 571
BseMI GCAATG 1 cut(s) 109
BseMII CTCAG 3 cut(s) 408, 500, 512
BseNI ACTGG 1 cut(s) 708
BseRI GAGGAG 1 cut(s) 770
BshFI GGCC 1 cut(s) 688
BsiHKAI GWGCWC 1 cut(s) 707
BsiSI CCGG 2 cut(s) 647, 689
BslFI GGGAC 1 cut(s) 179
BsmAI GTCTC 2 cut(s) 404, 413
BsmFI GGGAC 1 cut(s) 179
BsnI GGCC 1 cut(s) 688
Bsp1286I GDGCHC 1 cut(s) 707
Bsp143I GATC 6 cut(s) 316, 427, 543, 649, 730, 748
BspACI CCGC 3 cut(s) 563, 618, 684
BspANI GGCC 1 cut(s) 688
BspCNI CTCAG 3 cut(s) 409, 499, 511
BspOI GCTAGC 1 cut(s) 242
BspPI GGATC 2 cut(s) 422, 657
BsrDI GCAATG 1 cut(s) 109
BsrFI RCCGGY 1 cut(s) 688
BsrI ACTGG 1 cut(s) 708
BssAI RCCGGY 1 cut(s) 688
BssECI CCNNGG 3 cut(s) 126, 596, 763
BssMI GATC 6 cut(s) 316, 427, 543, 649, 730, 748
BssT1I CCWWGG 1 cut(s) 126
Bst2UI CCWGG 1 cut(s) 412
Bst4CI ACNGT 1 cut(s) 600
BstAPI GCANNNNNTGC 1 cut(s) 259
BstC8I GCNNGC 3 cut(s) 240, 620, 686
BstDEI CTNAG 5 cut(s) 133, 223, 417, 486, 498
BstDSI CCRYGG 1 cut(s) 596
BstF5I GGATG 3 cut(s) 85, 363, 571
BstH2I RGCGCY 2 cut(s) 290, 763
BstHHI GCGC 2 cut(s) 289, 762
BstKTI GATC 6 cut(s) 319, 430, 546, 652, 733, 751
BstMAI GTCTC 2 cut(s) 404, 413
BstMBI GATC 6 cut(s) 316, 427, 543, 649, 730, 748
BstMWI GCNNNNNNNGC 2 cut(s) 259, 517
BstNI CCWGG 1 cut(s) 412
BstSCI CCNGG 1 cut(s) 410
BstX2I RGATCY 1 cut(s) 748
BstYI RGATCY 1 cut(s) 748
BsuRI GGCC 1 cut(s) 688
BtgI CCRYGG 1 cut(s) 596
BtsCI GGATG 3 cut(s) 85, 363, 571
Cac8I GCNNGC 3 cut(s) 240, 620, 686
CfoI GCGC 2 cut(s) 289, 762
Cfr10I RCCGGY 1 cut(s) 688
Cfr13I GGNCC 3 cut(s) 64, 382, 686
CspCI CAANNNNNGTGG 3 cut(s) 38, 585, 620
CviAII CATG 3 cut(s) 283, 349, 541
CviJI RGCY 8 cut(s) 137, 230, 238, 242, 476, 536, 622, 688
CviKI_1 RGCY 8 cut(s) 137, 230, 238, 242, 476, 536, 622, 688
DdeI CTNAG 5 cut(s) 133, 223, 417, 486, 498
DpnI GATC 6 cut(s) 318, 429, 545, 651, 732, 750
DpnII GATC 6 cut(s) 316, 427, 543, 649, 730, 748
Eco130I CCWWGG 1 cut(s) 126
Eco47I GGWCC 2 cut(s) 64, 382
Eco57I CTGAAG 2 cut(s) 216, 483
EcoRII CCWGG 1 cut(s) 410
EcoT14I CCWWGG 1 cut(s) 126
EcoT22I ATGCAT 2 cut(s) 255, 396
ErhI CCWWGG 1 cut(s) 126
FaeI CATG 3 cut(s) 286, 352, 544
FaqI GGGAC 1 cut(s) 179
FatI CATG 3 cut(s) 282, 348, 540
FauI CCCGC 2 cut(s) 611, 677
FauNDI CATATG 1 cut(s) 313
FbaI TGATCA 2 cut(s) 543, 730
FokI GGATG 3 cut(s) 72, 370, 578
FspBI CTAG 2 cut(s) 239, 623
GlaI GCGC 2 cut(s) 288, 761
HaeII RGCGCY 2 cut(s) 290, 763
HaeIII GGCC 1 cut(s) 688
HapII CCGG 2 cut(s) 647, 689
HhaI GCGC 2 cut(s) 289, 762
Hin1II CATG 3 cut(s) 286, 352, 544
Hin6I GCGC 2 cut(s) 287, 760
HinP1I GCGC 2 cut(s) 287, 760
HincII GTYRAC 1 cut(s) 717
HindII GTYRAC 1 cut(s) 717
HinfI GANTC 1 cut(s) 76
HpaII CCGG 2 cut(s) 647, 689
HphI GGTGA 3 cut(s) 429, 437, 740
Hpy166II GTNNAC 2 cut(s) 382, 717
Hpy188I TCNGA 4 cut(s) 90, 177, 214, 235
Hpy188III TCNNGA 2 cut(s) 67, 752
Hpy8I GTNNAC 2 cut(s) 382, 717
HpyAV CCTTC 3 cut(s) 163, 167, 458
HpyCH4III ACNGT 1 cut(s) 600
HpyCH4IV ACGT 1 cut(s) 779
HpyCH4V TGCA 6 cut(s) 85, 102, 253, 394, 520, 607
HpyF10VI GCNNNNNNNGC 2 cut(s) 259, 517
HpyF3I CTNAG 5 cut(s) 133, 223, 417, 486, 498
HpySE526I ACGT 1 cut(s) 779
Hsp92II CATG 3 cut(s) 286, 352, 544
HspAI GCGC 2 cut(s) 287, 760
Ksp22I TGATCA 2 cut(s) 543, 730
Kzo9I GATC 6 cut(s) 316, 427, 543, 649, 730, 748
LmnI GCTCC 2 cut(s) 373, 757
LweI GCATC 2 cut(s) 94, 616
MaeI CTAG 2 cut(s) 239, 623
MaeII ACGT 1 cut(s) 779
MaeIII GTNAC 1 cut(s) 800
MalI GATC 6 cut(s) 318, 429, 545, 651, 732, 750
MboI GATC 6 cut(s) 316, 427, 543, 649, 730, 748
MboII GAAGA 1 cut(s) 352
MfeI CAATTG 1 cut(s) 80
MflI RGATCY 1 cut(s) 748
MhlI GDGCHC 1 cut(s) 707
MluCI AATT 4 cut(s) 25, 80, 257, 505
MlyI GAGTC 1 cut(s) 70
MmeI TCCRAC 3 cut(s) 185, 276, 303
MnlI CCTC 5 cut(s) 55, 260, 348, 748, 758
Mph1103I ATGCAT 2 cut(s) 255, 396
MroXI GAANNNNTTC 1 cut(s) 152
MslI CAYNNNNRTG 1 cut(s) 224
MspI CCGG 2 cut(s) 647, 689
MspR9I CCNGG 1 cut(s) 412
MunI CAATTG 1 cut(s) 80
MvaI CCWGG 1 cut(s) 412
MwoI GCNNNNNNNGC 2 cut(s) 259, 517
NdeI CATATG 1 cut(s) 313
NdeII GATC 6 cut(s) 316, 427, 543, 649, 730, 748
NheI GCTAGC 1 cut(s) 238
NlaIII CATG 3 cut(s) 286, 352, 544
NmeAIII GCCGAG 1 cut(s) 788
NsiI ATGCAT 2 cut(s) 255, 396
OliI CACNNNNGTG 1 cut(s) 224
PcsI WCGNNNNNNNCGW 1 cut(s) 325
PdmI GAANNNNTTC 1 cut(s) 152
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
PsiI TTATAA 1 cut(s) 114
Psp6I CCWGG 1 cut(s) 410
PspGI CCWGG 1 cut(s) 410
PspPI GGNCC 3 cut(s) 64, 382, 686
PsuI RGATCY 1 cut(s) 748
RseI CAYNNNNRTG 1 cut(s) 224
Sau3AI GATC 6 cut(s) 316, 427, 543, 649, 730, 748
Sau96I GGNCC 3 cut(s) 64, 382, 686
SchI GAGTC 1 cut(s) 70
ScrFI CCNGG 1 cut(s) 412
SduI GDGCHC 1 cut(s) 707
SfaNI GCATC 2 cut(s) 94, 616
SinI GGWCC 2 cut(s) 64, 382
SmiMI CAYNNNNRTG 1 cut(s) 224
SmlI CTYRAG 2 cut(s) 290, 752
SmoI CTYRAG 2 cut(s) 290, 752
Sse9I AATT 4 cut(s) 25, 80, 257, 505
SsiI CCGC 3 cut(s) 563, 618, 684
SspMI CTAG 2 cut(s) 239, 623
StyD4I CCNGG 1 cut(s) 410
StyI CCWWGG 1 cut(s) 126
TaaI ACNGT 1 cut(s) 600
TaiI ACGT 1 cut(s) 782
TaqI TCGA 2 cut(s) 695, 701
TasI AATT 4 cut(s) 25, 80, 257, 505
TspDTI ATGAA 3 cut(s) 17, 271, 780
VpaK11BI GGWCC 2 cut(s) 64, 382
XapI RAATTY 1 cut(s) 25
XmnI GAANNNNTTC 1 cut(s) 152
XspI CTAG 2 cut(s) 239, 623
Zsp2I ATGCAT 2 cut(s) 255, 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.