Prupe.3G150400_v2.0.a1

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000842
Physical Location & Seq
Forward (+)
16413430 .. 16414565
1136 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G150400.1

Sequence Viewer

Length: 942 bp
ATGCTTGTGGGTAAAGCAAAATCCTTTGGAGGGTTAGGGGTGGGTTCTTTGAAGGCTAGGAGTGCGGCTTTGCGGGCCAAATGGTTGTGGCGGTTTCCCAATGAACCTCACGCCCTTTGGCATAAAGTGATCAGAAGCATTTATGGAATGGATACAAATGAGTGGGATGCTAAACCCGTGATTCGAGGGTCTTGCCGTAGCCCTTGGAGAGACATTTCTAGTGGCTATAATTTGTTCCTTCAAGGCTATGTTTTCGTGGTAGGATGTGGAGTTAGGGTCAGATTTTGGGAGGACAATTGGAGCCGGGGTGGTGTTTTAAAAGAGGTGTTTCCAAGACTTTTCAATTTGTCCAGGAAGCAAAATCACAATATTTCCTCATATGTGGACTCGGATGGGTTCCCCCTCAGCTGGGATTTTGGTTTCAGGAGGAATCTCAATGATTTGGAGATTGCGGAGATGGCGGAGGTGGCTAGATTGTTGGACTTATTGGGGGGGATGAGGTTCTCTCCTTACACTCAAATTTGGAAGGCCAAGACTCCTTCGAAAGTTAAGATTTTTGTGTGGCAAGCGGTGTTGGGAAAACTAAATACAGATGATACATTACAGAGACACTGCACCTATATGTACCTTAGCCCTCACTGGTGTGCTCTCTGTAATAAGGCAGGGGAAAGTGCAGAACACCTCCTCCTTCATTGCCCTTTCTCTCTAAAGTTGTGGGGAAAGAAAGCTAAAATTCTCTGGGGCAGTCTGATGCAGGCAGTGATATGGAACCTTTGGCTGGAATGTAACAGGAGAATCTTCGAGGATTATAAGGGAGTGGGAGTGGTAGAGTCATGGGATAGAGTGAAGTTTTGGGCTGCCCTTTGGGCATCAACTTCCCTTGCTTTTAAGGATATATCCTATCCTACAATTATGCACAATCTGCTGGCTGTAGTATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 zf-RVT

Protein Analysis

314

Amino Acids

36.1

Weight (kDa)

9.57

Isoelectric Point (pI)

44.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000842)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15420 FvH4_2g00771 FvH4_3g29363
malus_domestica MD02G1293300.v1.1 MD06G1023000.v1.1 MD09G1232900.v1.1
prunus_persica Prupe.1G181500_v2.0.a1 Prupe.1G572100_v2.0.a1 Prupe.2G095500_v2.0.a1 Prupe.3G150400_v2.0.a1 Prupe.3G156200_v2.0.a1 Prupe.4G274400_v2.0.a1 Prupe.5G070700_v2.0.a1 Prupe.7G038000_v2.0.a1 Prupe.7G164000_v2.0.a1 Prupe.8G056500_v2.0.a1
pyrus_communis pycom04g02040 pycom05g04600 pycom07g05610 pycom08g16130 pycom11g16470 pycom12g07830 pycom15g38190 pycom215g00040
rosa_chinensis RchiOBHm_Chr1g0348881 RchiOBHm_Chr5g0032951 RchiOBHm_Chr6g0273271 RchiOBHm_Chr7g0218191 RchiOBHm_Chr7g0223721
rosa_multiflora Rmu_co8286615.1_g000001 Rmu_sc0001354.1_g000002 Rmu_sc0001925.1_g000003 Rmu_sc0001925.1_g000005 Rmu_sc0002221.1_g000004 Rmu_sc0002316.1_g000096 Rmu_sc0003291.1_g000005 Rmu_sc0003413.1_g000005 Rmu_sc0003542.1_g000011 Rmu_sc0004035.1_g000003 Rmu_sc0004035.1_g000004 Rmu_sc0004145.1_g000016 Rmu_sc0004660.1_g000004 Rmu_sc0005999.1_g000006 Rmu_sc0006123.1_g000002 Rmu_sc0006586.1_g000006 Rmu_sc0006889.1_g000029 Rmu_sc0008035.1_g000014 Rmu_sc0011962.1_g000002 Rmu_sc0029950.1_g000001 Rmu_sc0042409.1_g000001 Rmu_ssc0000238.1_g000013
rosa_roxburghii Rroxscaffold_2G00137410 Rroxscaffold_4G00312010 Rroxscaffold_4G00320800 Rroxscaffold_5G00341720 Rroxscaffold_5G00353530 Rroxscaffold_5G00360290 Rroxscaffold_7G00214170 Rroxscaffold_7G00215920
rosa_rugosa Rorug02G0157000
rosa_samantha Rh4BG196500 Rh6CG346400 Rh7BG241100 Rh7DG316300
rosa_wichuraiana Rw1G003610 Rw7G012170 Rw7G030150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 810
AciI CCGC 6 cut(s) 65, 73, 91, 452, 461, 569
AcsI RAATTY 2 cut(s) 519, 732
AfaI GTAC 1 cut(s) 626
AfiI CCNNNNNNNGG 4 cut(s) 30, 408, 409, 778
AgsI TTSAA 3 cut(s) 52, 242, 343
AjnI CCWGG 1 cut(s) 350
AleI CACNNNNGTG 1 cut(s) 642
AloI GAACNNNNNNTCC 2 cut(s) 669, 701
AluBI AGCT 2 cut(s) 408, 728
AluI AGCT 2 cut(s) 408, 728
Alw21I GWGCWC 1 cut(s) 649
Alw26I GTCTC 2 cut(s) 204, 601
AoxI GGCC 2 cut(s) 75, 528
ApeKI GCWGC 1 cut(s) 857
ApoI RAATTY 2 cut(s) 519, 732
AspS9I GGNCC 1 cut(s) 75
AsuC2I CCSGG 1 cut(s) 305
AsuII TTCGAA 1 cut(s) 542
Bbv12I GWGCWC 1 cut(s) 649
BbvCI CCTCAGC 1 cut(s) 404
BbvI GCAGC 1 cut(s) 844
BccI CCATC 2 cut(s) 386, 451
BceAI ACGGC 1 cut(s) 180
BcgI CGANNNNNNTGC 2 cut(s) 174, 208
BciT130I CCWGG 1 cut(s) 352
BciVI GTATCC 1 cut(s) 145
BclI TGATCA 1 cut(s) 129
BcnI CCSGG 1 cut(s) 305
BcoDI GTCTC 2 cut(s) 204, 601
BfaI CTAG 3 cut(s) 57, 219, 471
BfmI CTRYAG 1 cut(s) 930
BfuI GTATCC 1 cut(s) 145
BglI GCCNNNNNGGC 1 cut(s) 866
BisI GCNGC 2 cut(s) 66, 858
BlsI GCNGC 2 cut(s) 67, 859
Bme1390I CCNGG 2 cut(s) 305, 352
BmgT120I GGNCC 1 cut(s) 75
BmiI GGNNCC 3 cut(s) 302, 398, 770
BmrFI CCNGG 2 cut(s) 305, 352
BmsI GCATC 3 cut(s) 157, 741, 878
BplI GAGNNNNNCTC 2 cut(s) 490, 522
Bpu10I CCTNAGC 2 cut(s) 404, 629
Bpu14I TTCGAA 1 cut(s) 542
BpuMI CCSGG 1 cut(s) 305
BsaJI CCNNGG 2 cut(s) 203, 304
BsaXI ACNNNNNCTCC 8 cut(s) 261, 291, 292, 322, 669, 699, 807, 837
Bsc4I CCNNNNNNNGG 4 cut(s) 30, 408, 409, 778
Bse1I ACTGG 1 cut(s) 644
Bse3DI GCAATG 1 cut(s) 691
BseBI CCWGG 1 cut(s) 352
BseDI CCNNGG 2 cut(s) 203, 304
BseGI GGATG 4 cut(s) 172, 269, 397, 501
BseLI CCNNNNNNNGG 4 cut(s) 30, 408, 409, 778
BseMI GCAATG 1 cut(s) 691
BseMII CTCAG 1 cut(s) 418
BseNI ACTGG 1 cut(s) 644
BseRI GAGGAG 1 cut(s) 674
BseXI GCAGC 1 cut(s) 844
BseYI CCCAGC 1 cut(s) 408
BsgI GTGCAG 2 cut(s) 598, 693
BshFI GGCC 2 cut(s) 77, 530
BsiHKAI GWGCWC 1 cut(s) 649
BsiSI CCGG 1 cut(s) 304
BslI CCNNNNNNNGG 4 cut(s) 30, 408, 409, 778
BsmAI GTCTC 2 cut(s) 204, 601
BsnI GGCC 2 cut(s) 77, 530
Bsp119I TTCGAA 1 cut(s) 542
Bsp1286I GDGCHC 1 cut(s) 649
Bsp143I GATC 1 cut(s) 129
BspACI CCGC 6 cut(s) 65, 73, 91, 452, 461, 569
BspANI GGCC 2 cut(s) 77, 530
BspCNI CTCAG 1 cut(s) 417
BspLI GGNNCC 3 cut(s) 302, 398, 770
BspT104I TTCGAA 1 cut(s) 542
BsrDI GCAATG 1 cut(s) 691
BsrI ACTGG 1 cut(s) 644
BssECI CCNNGG 2 cut(s) 203, 304
BssMI GATC 1 cut(s) 129
BssT1I CCWWGG 1 cut(s) 203
Bst2UI CCWGG 1 cut(s) 352
BstAPI GCANNNNNTGC 1 cut(s) 922
BstBI TTCGAA 1 cut(s) 542
BstC8I GCNNGC 4 cut(s) 75, 567, 756, 927
BstDEI CTNAG 2 cut(s) 404, 629
BstF5I GGATG 4 cut(s) 172, 269, 397, 501
BstKTI GATC 1 cut(s) 132
BstMAI GTCTC 2 cut(s) 204, 601
BstMBI GATC 1 cut(s) 129
BstMWI GCNNNNNNNGC 6 cut(s) 62, 74, 458, 467, 866, 922
BstNI CCWGG 1 cut(s) 352
BstSCI CCNGG 2 cut(s) 303, 350
BstSFI CTRYAG 1 cut(s) 930
BstV1I GCAGC 1 cut(s) 844
BsuI GTATCC 1 cut(s) 145
BsuRI GGCC 2 cut(s) 77, 530
BtsCI GGATG 4 cut(s) 172, 269, 397, 501
BtsI GCAGTG 2 cut(s) 610, 765
BtsIMutI CAGTG 3 cut(s) 610, 637, 765
Cac8I GCNNGC 4 cut(s) 75, 567, 756, 927
Cfr13I GGNCC 1 cut(s) 75
Csp6I GTAC 1 cut(s) 625
CviAII CATG 1 cut(s) 834
CviQI GTAC 1 cut(s) 625
DdeI CTNAG 2 cut(s) 404, 629
DpnI GATC 1 cut(s) 131
DpnII GATC 1 cut(s) 129
DraI TTTAAA 1 cut(s) 318
EciI GGCGGA 1 cut(s) 476
Eco130I CCWWGG 1 cut(s) 203
EcoRII CCWGG 1 cut(s) 350
EcoT14I CCWWGG 1 cut(s) 203
ErhI CCWWGG 1 cut(s) 203
FaeI CATG 1 cut(s) 837
FatI CATG 1 cut(s) 833
FauI CCCGC 1 cut(s) 66
FauNDI CATATG 1 cut(s) 379
FbaI TGATCA 1 cut(s) 129
Fnu4HI GCNGC 2 cut(s) 66, 858
FokI GGATG 4 cut(s) 179, 276, 404, 508
Fsp4HI GCNGC 2 cut(s) 66, 858
FspBI CTAG 3 cut(s) 57, 219, 471
GluI GCNGC 2 cut(s) 66, 858
GsaI CCCAGC 1 cut(s) 412
HaeIII GGCC 2 cut(s) 77, 530
HapII CCGG 1 cut(s) 304
Hin1II CATG 1 cut(s) 837
HinfI GANTC 6 cut(s) 181, 386, 430, 535, 795, 830
HpaII CCGG 1 cut(s) 304
Hpy166II GTNNAC 1 cut(s) 385
Hpy188I TCNGA 4 cut(s) 134, 281, 391, 750
Hpy188III TCNNGA 1 cut(s) 424
Hpy8I GTNNAC 1 cut(s) 385
HpyAV CCTTC 5 cut(s) 46, 248, 520, 549, 698
HpyCH4V TGCA 4 cut(s) 615, 674, 754, 916
HpyF10VI GCNNNNNNNGC 6 cut(s) 62, 74, 458, 467, 866, 922
HpyF3I CTNAG 2 cut(s) 404, 629
Hsp92II CATG 1 cut(s) 837
Ksp22I TGATCA 1 cut(s) 129
Kzo9I GATC 1 cut(s) 129
LmnI GCTCC 1 cut(s) 300
Lsp1109I GCAGC 1 cut(s) 844
LweI GCATC 3 cut(s) 157, 741, 878
MaeI CTAG 3 cut(s) 57, 219, 471
MaeIII GTNAC 1 cut(s) 785
MalI GATC 1 cut(s) 131
MboI GATC 1 cut(s) 129
MboII GAAGA 1 cut(s) 790
MfeI CAATTG 1 cut(s) 295
MhlI GDGCHC 1 cut(s) 649
MluCI AATT 6 cut(s) 229, 295, 343, 519, 732, 909
MlyI GAGTC 3 cut(s) 380, 529, 839
MmeI TCCRAC 1 cut(s) 459
MseI TTAA 4 cut(s) 317, 549, 888, 940
MslI CAYNNNNRTG 2 cut(s) 620, 642
MspA1I CMGCKG 1 cut(s) 408
MspI CCGG 1 cut(s) 304
MspR9I CCNGG 2 cut(s) 305, 352
MunI CAATTG 1 cut(s) 295
MvaI CCWGG 1 cut(s) 352
MwoI GCNNNNNNNGC 6 cut(s) 62, 74, 458, 467, 866, 922
NciI CCSGG 1 cut(s) 305
NdeI CATATG 1 cut(s) 379
NdeII GATC 1 cut(s) 129
NlaIII CATG 1 cut(s) 837
NlaIV GGNNCC 3 cut(s) 302, 398, 770
NspV TTCGAA 1 cut(s) 542
OliI CACNNNNGTG 1 cut(s) 642
PfeI GAWTC 3 cut(s) 181, 430, 795
PfoI TCCNGGA 1 cut(s) 350
PkrI GCNGC 2 cut(s) 67, 859
PleI GAGTC 3 cut(s) 380, 529, 838
PpsI GAGTC 3 cut(s) 380, 529, 838
PsiI TTATAA 1 cut(s) 810
Psp6I CCWGG 1 cut(s) 350
PspFI CCCAGC 1 cut(s) 408
PspGI CCWGG 1 cut(s) 350
PspN4I GGNNCC 3 cut(s) 302, 398, 770
PspPI GGNCC 1 cut(s) 75
PvuII CAGCTG 1 cut(s) 408
RsaI GTAC 1 cut(s) 626
RsaNI GTAC 1 cut(s) 625
RseI CAYNNNNRTG 2 cut(s) 620, 642
SaqAI TTAA 4 cut(s) 317, 549, 888, 940
SatI GCNGC 2 cut(s) 66, 858
Sau3AI GATC 1 cut(s) 129
Sau96I GGNCC 1 cut(s) 75
SchI GAGTC 3 cut(s) 380, 529, 839
ScrFI CCNGG 2 cut(s) 305, 352
SduI GDGCHC 1 cut(s) 649
SfaNI GCATC 3 cut(s) 157, 741, 878
SfcI CTRYAG 1 cut(s) 930
SfuI TTCGAA 1 cut(s) 542
SmiMI CAYNNNNRTG 2 cut(s) 620, 642
Sse9I AATT 6 cut(s) 229, 295, 343, 519, 732, 909
SsiI CCGC 6 cut(s) 65, 73, 91, 452, 461, 569
SspI AATATT 1 cut(s) 370
SspMI CTAG 3 cut(s) 57, 219, 471
StyD4I CCNGG 2 cut(s) 303, 350
StyI CCWWGG 1 cut(s) 203
TaqI TCGA 3 cut(s) 184, 542, 801
TasI AATT 6 cut(s) 229, 295, 343, 519, 732, 909
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 3 cut(s) 181, 430, 795
Tru1I TTAA 4 cut(s) 317, 549, 888, 940
Tru9I TTAA 4 cut(s) 317, 549, 888, 940
TscAI CASTG 3 cut(s) 617, 644, 765
TseI GCWGC 1 cut(s) 857
TspDTI ATGAA 2 cut(s) 117, 680
TspRI CASTG 3 cut(s) 617, 644, 765
XapI RAATTY 2 cut(s) 519, 732
XspI CTAG 3 cut(s) 57, 219, 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.