FvH4_3g29014

No apical meristem-associated C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001050
Physical Location & Seq
Forward (+)
22135789 .. 22137078
1290 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g29014.t1

Sequence Viewer

Length: 945 bp
ATGGATTTGACACCATTGAATTTCACTAACCTCCTCAATAGTAGCAAATCAGCCCTTGATGATCTCAATGAACCATTGCATGCATACGATTTTCCATTGAGCCAGTGTCATCTCAATGATTCTCCTTATGAAGAGATATGTAGAACTGAATTTTGTGCTACTGAAGAGCAGCAGTCACCGTGTGAACCTAGCAGCAAGAAAGCTGCTGCACCACGACAAGCTAACTTCTCAATTGAGGAAGACAAACTTCTTGTATCTGCATGGCTCAATATCAGCTTGGACCCTGTCAAAGGAACTGGCCAAAAGAGACAACAATTCTGGAAAAGAATTACAAAATACTTTGAAGACAACAAGACGTGGACTGGTGAACGGTCGACAAAATCACTCATTAATCGTTGGTCAACAATCAGCAGCAGTGTGAGCAAGTTTTGTGGACATTATTCTTCAATTGAACACTTGAGGAGAAGTGGTTACACAGAACAAGATAAGATAGTGGAGGCAAAAGAGACTTACAAGAAAGAAGAACGTCATGCATTTGTACTTGATCATTGTTGGGTTGTTTTGAGGTTCCAGCAAAAATGGATGGATGAACATCAAAATCAAGAATCTAAGAGACAACAACAGAAGGTTCGTTCTAGTAAGAATCATGATTCAATCAACTTAGAAGATGATTTGGAAGCTCCTGAACCTGCAAGCTTGAAGAGGCCAATTGGGAGAAAGGCTGCAAAAGAAGCAAACAAGAAAGCAAAGTCCAACGATGAAGGTAGCGCAAATGAGAAGGAATCTAAAATCCGTTCTGAGTTGGAAGAGTTCAGAGCAAAGCGATTAGAAAGTGATCGCATAAAGGCTGAACAATTTAAGTTGCTACTTGATACTCAGAAAAAGGAAGTTGCTCTTAAGAGAAGGAACTTGAGCTTCAGGCTCGGAGTGAGGAGATTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM-associated

Protein Analysis

315

Amino Acids

36.58

Weight (kDa)

8.84

Isoelectric Point (pI)

67.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM-associated PF14303 178 - 285 5.1e-11 No apical meristem-associated C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 697
AccI GTMKAC 1 cut(s) 374
AcoI YGGCCR 1 cut(s) 298
AcsI RAATTY 2 cut(s) 19, 149
AcuI CTGAAG 2 cut(s) 183, 901
AdeI CACNNNGTG 1 cut(s) 182
AfaI GTAC 1 cut(s) 540
AfiI CCNNNNNNNGG 1 cut(s) 290
AflII CTTAAG 1 cut(s) 896
AgsI TTSAA 6 cut(s) 19, 344, 447, 452, 654, 700
AjiI CACGTC 1 cut(s) 357
AluBI AGCT 6 cut(s) 203, 221, 276, 680, 696, 915
AluI AGCT 6 cut(s) 203, 221, 276, 680, 696, 915
Alw26I GTCTC 3 cut(s) 301, 500, 607
AoxI GGCC 2 cut(s) 298, 704
ApeKI GCWGC 6 cut(s) 169, 192, 203, 206, 411, 722
ApoI RAATTY 2 cut(s) 19, 149
AseI ATTAAT 1 cut(s) 390
AspLEI GCGC 1 cut(s) 770
AspS9I GGNCC 1 cut(s) 280
AsuHPI GGTGA 2 cut(s) 168, 377
AvaII GGWCC 1 cut(s) 280
BalI TGGCCA 1 cut(s) 300
BbsI GAAGAC 2 cut(s) 246, 351
BbvI GCAGC 6 cut(s) 181, 190, 193, 204, 423, 709
BccI CCATC 1 cut(s) 577
BclI TGATCA 1 cut(s) 544
BcoDI GTCTC 3 cut(s) 301, 500, 607
BfaI CTAG 2 cut(s) 189, 636
BfrI CTTAAG 1 cut(s) 896
BfuAI ACCTGC 1 cut(s) 697
BisI GCNGC 6 cut(s) 170, 193, 204, 207, 412, 723
BlsI GCNGC 6 cut(s) 171, 194, 205, 208, 413, 724
Bme18I GGWCC 1 cut(s) 280
BmgBI CACGTC 1 cut(s) 357
BmgT120I GGNCC 1 cut(s) 280
BmiI GGNNCC 2 cut(s) 282, 569
BpiI GAAGAC 2 cut(s) 246, 351
BpuEI CTTGAG 2 cut(s) 478, 931
BsaBI GATNNNNATC 1 cut(s) 591
BsaXI ACNNNNNCTCC 2 cut(s) 454, 484
Bsc4I CCNNNNNNNGG 1 cut(s) 290
Bse1I ACTGG 3 cut(s) 103, 301, 367
Bse3DI GCAATG 1 cut(s) 74
Bse8I GATNNNNATC 1 cut(s) 591
BseGI GGATG 2 cut(s) 588, 592
BseJI GATNNNNATC 1 cut(s) 591
BseLI CCNNNNNNNGG 1 cut(s) 290
BseMI GCAATG 1 cut(s) 74
BseMII CTCAG 2 cut(s) 789, 890
BseNI ACTGG 3 cut(s) 103, 301, 367
BseRI GAGGAG 2 cut(s) 23, 475
BseXI GCAGC 6 cut(s) 181, 190, 193, 204, 423, 709
BsgI GTGCAG 1 cut(s) 192
Bsh1285I CGRYCG 1 cut(s) 374
BshFI GGCC 2 cut(s) 300, 706
BsiEI CGRYCG 1 cut(s) 374
BslI CCNNNNNNNGG 1 cut(s) 290
BsmAI GTCTC 3 cut(s) 301, 500, 607
BsnI GGCC 2 cut(s) 300, 706
Bsp143I GATC 3 cut(s) 61, 544, 835
BspANI GGCC 2 cut(s) 300, 706
BspCNI CTCAG 2 cut(s) 790, 889
BspHI TCATGA 1 cut(s) 646
BspLI GGNNCC 2 cut(s) 282, 569
BspMI ACCTGC 1 cut(s) 697
BspQI GCTCTTC 1 cut(s) 159
BspTI CTTAAG 1 cut(s) 896
BsrDI GCAATG 1 cut(s) 74
BsrI ACTGG 3 cut(s) 103, 301, 367
BssMI GATC 3 cut(s) 61, 544, 835
Bst4CI ACNGT 2 cut(s) 180, 372
Bst6I CTCTTC 4 cut(s) 126, 159, 695, 801
BstAFI CTTAAG 1 cut(s) 896
BstC8I GCNNGC 2 cut(s) 81, 694
BstDEI CTNAG 4 cut(s) 609, 661, 798, 876
BstENI CCTNNNNNAGG 1 cut(s) 288
BstF5I GGATG 2 cut(s) 588, 592
BstHHI GCGC 1 cut(s) 770
BstKTI GATC 3 cut(s) 64, 547, 838
BstMAI GTCTC 3 cut(s) 301, 500, 607
BstMBI GATC 3 cut(s) 61, 544, 835
BstMCI CGRYCG 1 cut(s) 374
BstMWI GCNNNNNNNGC 2 cut(s) 420, 731
BstNSI RCATGY 1 cut(s) 83
BstV1I GCAGC 6 cut(s) 181, 190, 193, 204, 423, 709
BstV2I GAAGAC 2 cut(s) 246, 351
BsuRI GGCC 2 cut(s) 300, 706
BtrI CACGTC 1 cut(s) 357
BtsCI GGATG 2 cut(s) 588, 592
BtsI GCAGTG 1 cut(s) 421
BtsIMutI CAGTG 2 cut(s) 110, 421
BveI ACCTGC 1 cut(s) 697
Cac8I GCNNGC 2 cut(s) 81, 694
CciI TCATGA 1 cut(s) 646
CfoI GCGC 1 cut(s) 770
Cfr13I GGNCC 1 cut(s) 280
Csp6I GTAC 1 cut(s) 539
CspCI CAANNNNNGTGG 2 cut(s) 412, 447
CviAII CATG 4 cut(s) 80, 261, 530, 647
CviQI GTAC 1 cut(s) 539
DdeI CTNAG 4 cut(s) 609, 661, 798, 876
DpnI GATC 3 cut(s) 63, 546, 837
DpnII GATC 3 cut(s) 61, 544, 835
DraIII CACNNNGTG 1 cut(s) 182
EaeI YGGCCR 1 cut(s) 298
Eam1104I CTCTTC 4 cut(s) 126, 159, 695, 801
EarI CTCTTC 4 cut(s) 126, 159, 695, 801
Eco47I GGWCC 1 cut(s) 280
Eco57I CTGAAG 2 cut(s) 183, 901
EcoNI CCTNNNNNAGG 1 cut(s) 288
EcoT22I ATGCAT 2 cut(s) 85, 535
FaeI CATG 4 cut(s) 83, 264, 533, 650
FaiI YATR 8 cut(s) 81, 85, 129, 139, 262, 531, 648, 842
FalI AAGNNNNNCTT 4 cut(s) 231, 263, 879, 911
FatI CATG 4 cut(s) 79, 260, 529, 646
FbaI TGATCA 1 cut(s) 544
FblI GTMKAC 1 cut(s) 374
Fnu4HI GCNGC 6 cut(s) 170, 193, 204, 207, 412, 723
FokI GGATG 2 cut(s) 595, 599
Fsp4HI GCNGC 6 cut(s) 170, 193, 204, 207, 412, 723
FspBI CTAG 2 cut(s) 189, 636
GlaI GCGC 1 cut(s) 769
GluI GCNGC 6 cut(s) 170, 193, 204, 207, 412, 723
HaeIII GGCC 2 cut(s) 300, 706
HhaI GCGC 1 cut(s) 770
Hin1II CATG 4 cut(s) 83, 264, 533, 650
Hin6I GCGC 1 cut(s) 768
HinP1I GCGC 1 cut(s) 768
HincII GTYRAC 2 cut(s) 375, 402
HindII GTYRAC 2 cut(s) 375, 402
HindIII AAGCTT 1 cut(s) 694
HinfI GANTC 5 cut(s) 119, 605, 643, 650, 782
HphI GGTGA 2 cut(s) 168, 377
Hpy166II GTNNAC 6 cut(s) 185, 360, 368, 375, 402, 434
Hpy188I TCNGA 4 cut(s) 799, 815, 879, 926
Hpy188III TCNNGA 4 cut(s) 319, 602, 647, 683
Hpy8I GTNNAC 6 cut(s) 185, 360, 368, 375, 402, 434
HpyAV CCTTC 4 cut(s) 619, 755, 772, 897
HpyCH4III ACNGT 2 cut(s) 180, 372
HpyCH4IV ACGT 2 cut(s) 356, 526
HpyCH4V TGCA 7 cut(s) 79, 83, 209, 260, 533, 692, 725
HpyF10VI GCNNNNNNNGC 2 cut(s) 420, 731
HpyF3I CTNAG 4 cut(s) 609, 661, 798, 876
HpySE526I ACGT 2 cut(s) 356, 526
Hsp92II CATG 4 cut(s) 83, 264, 533, 650
HspAI GCGC 1 cut(s) 768
Ksp22I TGATCA 1 cut(s) 544
Kzo9I GATC 3 cut(s) 61, 544, 835
LguI GCTCTTC 1 cut(s) 159
LmnI GCTCC 1 cut(s) 685
LpnPI CCDG 9 cut(s) 116, 282, 297, 304, 348, 584, 696, 702, 904
Lsp1109I GCAGC 6 cut(s) 181, 190, 193, 204, 423, 709
MaeI CTAG 2 cut(s) 189, 636
MaeII ACGT 2 cut(s) 356, 526
MaeIII GTNAC 2 cut(s) 174, 470
MalI GATC 3 cut(s) 63, 546, 837
MboI GATC 3 cut(s) 61, 544, 835
MboII GAAGA 9 cut(s) 143, 176, 251, 356, 435, 533, 677, 712, 818
MfeI CAATTG 3 cut(s) 231, 447, 708
MlsI TGGCCA 1 cut(s) 300
MluCI AATT 8 cut(s) 19, 149, 231, 314, 327, 447, 708, 854
MluNI TGGCCA 1 cut(s) 300
MmeI TCCRAC 2 cut(s) 777, 783
MnlI CCTC 8 cut(s) 41, 44, 229, 453, 490, 558, 696, 924
Mox20I TGGCCA 1 cut(s) 300
Mph1103I ATGCAT 2 cut(s) 85, 535
MscI TGGCCA 1 cut(s) 300
MseI TTAA 3 cut(s) 390, 858, 897
MslI CAYNNNNRTG 1 cut(s) 114
Msp20I TGGCCA 1 cut(s) 300
MspCI CTTAAG 1 cut(s) 896
MunI CAATTG 3 cut(s) 231, 447, 708
MwoI GCNNNNNNNGC 2 cut(s) 420, 731
NdeII GATC 3 cut(s) 61, 544, 835
NlaIII CATG 4 cut(s) 83, 264, 533, 650
NlaIV GGNNCC 2 cut(s) 282, 569
NmuCI GTSAC 1 cut(s) 174
NsiI ATGCAT 2 cut(s) 85, 535
NspI RCATGY 1 cut(s) 83
PaeI GCATGC 1 cut(s) 83
PagI TCATGA 1 cut(s) 646
PciSI GCTCTTC 1 cut(s) 159
PfeI GAWTC 5 cut(s) 119, 605, 643, 650, 782
PflFI GACNNNGTC 1 cut(s) 284
PkrI GCNGC 6 cut(s) 171, 194, 205, 208, 413, 724
PshBI ATTAAT 1 cut(s) 390
PspN4I GGNNCC 2 cut(s) 282, 569
PspPI GGNCC 1 cut(s) 280
PsyI GACNNNGTC 1 cut(s) 284
RsaI GTAC 1 cut(s) 540
RsaNI GTAC 1 cut(s) 539
RseI CAYNNNNRTG 1 cut(s) 114
SalI GTCGAC 1 cut(s) 373
SapI GCTCTTC 1 cut(s) 159
SaqAI TTAA 3 cut(s) 390, 858, 897
SatI GCNGC 6 cut(s) 170, 193, 204, 207, 412, 723
Sau3AI GATC 3 cut(s) 61, 544, 835
Sau96I GGNCC 1 cut(s) 280
SinI GGWCC 1 cut(s) 280
SmiMI CAYNNNNRTG 1 cut(s) 114
SmlI CTYRAG 3 cut(s) 457, 896, 910
SmoI CTYRAG 3 cut(s) 457, 896, 910
SphI GCATGC 1 cut(s) 83
Sse9I AATT 8 cut(s) 19, 149, 231, 314, 327, 447, 708, 854
SspMI CTAG 2 cut(s) 189, 636
TaaI ACNGT 2 cut(s) 180, 372
TaiI ACGT 2 cut(s) 359, 529
TaqI TCGA 1 cut(s) 374
TasI AATT 8 cut(s) 19, 149, 231, 314, 327, 447, 708, 854
TatI WGTACW 1 cut(s) 538
TfiI GAWTC 5 cut(s) 119, 605, 643, 650, 782
Tru1I TTAA 3 cut(s) 390, 858, 897
Tru9I TTAA 3 cut(s) 390, 858, 897
TscAI CASTG 2 cut(s) 110, 421
TseFI GTSAC 1 cut(s) 174
TseI GCWGC 6 cut(s) 169, 192, 203, 206, 411, 722
Tsp45I GTSAC 1 cut(s) 174
TspDTI ATGAA 4 cut(s) 84, 144, 603, 774
TspGWI ACGGA 1 cut(s) 782
TspRI CASTG 2 cut(s) 110, 421
Tth111I GACNNNGTC 1 cut(s) 284
Vha464I CTTAAG 1 cut(s) 896
VpaK11BI GGWCC 1 cut(s) 280
VspI ATTAAT 1 cut(s) 390
XagI CCTNNNNNAGG 1 cut(s) 288
XapI RAATTY 2 cut(s) 19, 149
XceI RCATGY 1 cut(s) 83
XmiI GTMKAC 1 cut(s) 374
XspI CTAG 2 cut(s) 189, 636
Zsp2I ATGCAT 2 cut(s) 85, 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.