Rroxscaffold_4G00321120

No apical meristem-associated C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001050
Physical Location & Seq
Forward (+)
50317088 .. 50320233
3146 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00321120.1

Sequence Viewer

Length: 1044 bp
ATGGACTACGCCAACAGTGAAACAAACCGCAATGAACCTCTTCTGACATATGATTTCCCGTCGAGTCAATATCACACCAATGGTTCTACTTGTGCTCCTATACCATTTGAAGATTGCACTTATGAAGAGACACTTACTAATGAAGATTGCACTAATAAAGAGACAACTTGTGCTCCTGGCAGCAAGAAAGCACAAAGACAAGCTAACTTCACGGTCGAAGAAGACAAACTTCTTATATCTGCATGGCTCAATGTTAGCATAGACCAAGTGAAAGGAGCTGATCAAAAGAAAAAACAATTTTGGAAAAGAATTACTAAATACTTTGAAGATAACAAGAAGTGGTCTGGTGAACGGAGTGAAAAGTCATTAACGAACCGTTGGTCAAAAATCAATACCACTGTGAGTAAGTTTTATGGGCATCATTCTCAAATTGAACACTTACAGAAAAGTGATTACACGGAACAAGATAAGATAGTGGAGGCAAAAGAGATGTTCAAGAAAAAGGAAGGTCATACATTTGTACTTGACCATTGTTGGACTATTATGAGGTTCCAGCAAAAATGGATGGATGAACATCAAAAAATCAATGCCAAGAGAAAGTTCAAGGCTCCTGATTCAATCAATTTAGAAGATGATGATGTAGAAGGTCCTGAACCTGCACCCTTGAAGAGGCCAATTGGGAGAAAGGCTGCAAAGGAAGTGATCAAAAAGGCAAAATCCAAAGAACAGGTTAGTGAAAATGAGACACCTTCAAAAAGTACTTCTGAGTTGGAAGAGTTTATGGCAAAAAAATTAGAAAGTGAACGCATAAGGGCGGAACAATTTAAGTTGCTACTTGCTACTGAGCAAAAGCAAGTTGCTCTTAGAGAAAAGGAAATTGAAATTCAGCTTCGAAGTGAGGATGCTAAAATAATGGCCATGGATACTAGTGTCATGCCTCCAATTCAAGCAGAATACTTTATTGGTCTTCAGAAGGAAATCTTGGCAAAAAGAGCTAGCGGCGGTGGAAATCAAGTGTTTCTTTCTGAATGTACAATAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM-associated

Protein Analysis

347

Amino Acids

40.15

Weight (kDa)

7.06

Isoelectric Point (pI)

52.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM-associated PF14303 172 - 327 4.2e-14 No apical meristem-associated C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 664
AciI CCGC 4 cut(s) 28, 815, 999, 1002
AcoI YGGCCR 1 cut(s) 915
AcsI RAATTY 1 cut(s) 882
AcuI CTGAAG 1 cut(s) 953
AfaI GTAC 3 cut(s) 522, 760, 1033
AfiI CCNNNNNNNGG 1 cut(s) 669
AhlI ACTAGT 1 cut(s) 926
AjnI CCWGG 1 cut(s) 175
AjuI GAANNNNNNNTTGG 6 cut(s) 584, 616, 945, 965, 977, 997
AluBI AGCT 4 cut(s) 203, 278, 889, 995
AluI AGCT 4 cut(s) 203, 278, 889, 995
Alw21I GWGCWC 2 cut(s) 97, 175
Alw26I GTCTC 3 cut(s) 122, 155, 737
AoxI GGCC 2 cut(s) 671, 915
ApeKI GCWGC 2 cut(s) 180, 689
ApoI RAATTY 1 cut(s) 882
ArsI GACNNNNNNTTYG 2 cut(s) 365, 397
Asp700I GAANNNNTTC 1 cut(s) 39
AspS9I GGNCC 1 cut(s) 647
AsuHPI GGTGA 1 cut(s) 359
AsuII TTCGAA 1 cut(s) 892
AsuNHI GCTAGC 1 cut(s) 995
AvaII GGWCC 1 cut(s) 647
BaeI ACNNNNGTAYC 2 cut(s) 915, 948
BalI TGGCCA 1 cut(s) 917
BbsI GAAGAC 2 cut(s) 228, 959
Bbv12I GWGCWC 2 cut(s) 97, 175
BbvI GCAGC 2 cut(s) 192, 676
BccI CCATC 1 cut(s) 559
BciT130I CCWGG 1 cut(s) 177
BciVI GTATCC 1 cut(s) 916
BclI TGATCA 2 cut(s) 280, 702
BcoDI GTCTC 3 cut(s) 122, 155, 737
BcuI ACTAGT 1 cut(s) 926
BfaI CTAG 2 cut(s) 927, 996
BfuAI ACCTGC 1 cut(s) 664
BfuI GTATCC 1 cut(s) 916
BisI GCNGC 3 cut(s) 181, 690, 1000
BlsI GCNGC 3 cut(s) 182, 691, 1001
BmcAI AGTACT 1 cut(s) 760
Bme1390I CCNGG 1 cut(s) 177
Bme18I GGWCC 1 cut(s) 647
BmgT120I GGNCC 1 cut(s) 647
BmiI GGNNCC 2 cut(s) 551, 609
BmrFI CCNGG 1 cut(s) 177
BmsI GCATC 2 cut(s) 427, 892
BmtI GCTAGC 1 cut(s) 999
BpiI GAAGAC 2 cut(s) 228, 959
Bpu14I TTCGAA 1 cut(s) 892
BsaBI GATNNNNATC 1 cut(s) 573
BsaJI CCNNGG 1 cut(s) 918
BsaXI ACNNNNNCTCC 4 cut(s) 79, 109, 157, 187
Bsc4I CCNNNNNNNGG 1 cut(s) 669
Bse3DI GCAATG 1 cut(s) 37
Bse8I GATNNNNATC 1 cut(s) 573
BseBI CCWGG 1 cut(s) 177
BseDI CCNNGG 1 cut(s) 918
BseGI GGATG 3 cut(s) 570, 574, 907
BseJI GATNNNNATC 1 cut(s) 573
BseLI CCNNNNNNNGG 1 cut(s) 669
BseMI GCAATG 1 cut(s) 37
BseMII CTCAG 2 cut(s) 756, 834
BseXI GCAGC 2 cut(s) 192, 676
BsgI GTGCAG 1 cut(s) 642
Bsh1285I CGRYCG 1 cut(s) 216
BshFI GGCC 2 cut(s) 673, 917
BsiEI CGRYCG 1 cut(s) 216
BsiHKAI GWGCWC 2 cut(s) 97, 175
BslI CCNNNNNNNGG 1 cut(s) 669
BsmAI GTCTC 3 cut(s) 122, 155, 737
BsnI GGCC 2 cut(s) 673, 917
Bsp119I TTCGAA 1 cut(s) 892
Bsp1286I GDGCHC 2 cut(s) 97, 175
Bsp1407I TGTACA 1 cut(s) 1031
Bsp143I GATC 2 cut(s) 280, 702
Bsp19I CCATGG 1 cut(s) 918
BspACI CCGC 4 cut(s) 28, 815, 999, 1002
BspANI GGCC 2 cut(s) 673, 917
BspCNI CTCAG 2 cut(s) 757, 835
BspLI GGNNCC 2 cut(s) 551, 609
BspMI ACCTGC 1 cut(s) 664
BspOI GCTAGC 1 cut(s) 999
BspT104I TTCGAA 1 cut(s) 892
BsrDI GCAATG 1 cut(s) 37
BsrGI TGTACA 1 cut(s) 1031
BssECI CCNNGG 1 cut(s) 918
BssMI GATC 2 cut(s) 280, 702
BssT1I CCWWGG 1 cut(s) 918
Bst2UI CCWGG 1 cut(s) 177
Bst4CI ACNGT 4 cut(s) 17, 214, 377, 400
Bst6I CTCTTC 4 cut(s) 45, 120, 662, 768
BstAUI TGTACA 1 cut(s) 1031
BstBI TTCGAA 1 cut(s) 892
BstC8I GCNNGC 1 cut(s) 997
BstDEI CTNAG 3 cut(s) 765, 843, 863
BstDSI CCRYGG 1 cut(s) 918
BstENI CCTNNNNNAGG 1 cut(s) 667
BstF5I GGATG 3 cut(s) 570, 574, 907
BstKTI GATC 2 cut(s) 283, 705
BstMAI GTCTC 3 cut(s) 122, 155, 737
BstMBI GATC 2 cut(s) 280, 702
BstMCI CGRYCG 1 cut(s) 216
BstMWI GCNNNNNNNGC 1 cut(s) 992
BstNI CCWGG 1 cut(s) 177
BstSCI CCNGG 1 cut(s) 175
BstV1I GCAGC 2 cut(s) 192, 676
BstV2I GAAGAC 2 cut(s) 228, 959
BsuI GTATCC 1 cut(s) 916
BsuRI GGCC 2 cut(s) 673, 917
BtgI CCRYGG 1 cut(s) 918
BtsCI GGATG 3 cut(s) 570, 574, 907
BtsIMutI CAGTG 2 cut(s) 22, 396
BveI ACCTGC 1 cut(s) 664
Cac8I GCNNGC 1 cut(s) 997
Cfr13I GGNCC 1 cut(s) 647
Csp6I GTAC 3 cut(s) 521, 759, 1032
CviAII CATG 3 cut(s) 243, 919, 934
CviJI RGCY 9 cut(s) 203, 247, 278, 608, 673, 689, 889, 917, 995
CviKI_1 RGCY 9 cut(s) 203, 247, 278, 608, 673, 689, 889, 917, 995
CviQI GTAC 3 cut(s) 521, 759, 1032
DdeI CTNAG 3 cut(s) 765, 843, 863
DpnI GATC 2 cut(s) 282, 704
DpnII GATC 2 cut(s) 280, 702
EaeI YGGCCR 1 cut(s) 915
Eam1104I CTCTTC 4 cut(s) 45, 120, 662, 768
EarI CTCTTC 4 cut(s) 45, 120, 662, 768
EciI GGCGGA 1 cut(s) 830
Eco130I CCWWGG 1 cut(s) 918
Eco47I GGWCC 1 cut(s) 647
Eco57I CTGAAG 1 cut(s) 953
EcoNI CCTNNNNNAGG 1 cut(s) 667
EcoO109I RGGNCCY 1 cut(s) 647
EcoRII CCWGG 1 cut(s) 175
EcoT14I CCWWGG 1 cut(s) 918
ErhI CCWWGG 1 cut(s) 918
FaeI CATG 3 cut(s) 246, 922, 937
FatI CATG 3 cut(s) 242, 918, 933
FauNDI CATATG 1 cut(s) 49
FbaI TGATCA 2 cut(s) 280, 702
Fnu4HI GCNGC 3 cut(s) 181, 690, 1000
FokI GGATG 3 cut(s) 577, 581, 914
Fsp4HI GCNGC 3 cut(s) 181, 690, 1000
FspBI CTAG 2 cut(s) 927, 996
GluI GCNGC 3 cut(s) 181, 690, 1000
HaeIII GGCC 2 cut(s) 673, 917
Hin1II CATG 3 cut(s) 246, 922, 937
HinfI GANTC 2 cut(s) 64, 614
HphI GGTGA 1 cut(s) 359
Hpy166II GTNNAC 2 cut(s) 350, 803
Hpy188I TCNGA 4 cut(s) 45, 766, 972, 1027
Hpy188III TCNNGA 3 cut(s) 496, 611, 650
Hpy8I GTNNAC 2 cut(s) 350, 803
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 4 cut(s) 500, 638, 759, 967
HpyCH4III ACNGT 4 cut(s) 17, 214, 377, 400
HpyCH4V TGCA 5 cut(s) 117, 150, 242, 659, 692
HpyF10VI GCNNNNNNNGC 1 cut(s) 992
HpyF3I CTNAG 3 cut(s) 765, 843, 863
Hsp92II CATG 3 cut(s) 246, 922, 937
Ksp22I TGATCA 2 cut(s) 280, 702
Kzo9I GATC 2 cut(s) 280, 702
LmnI GCTCC 4 cut(s) 100, 178, 275, 613
LpnPI CCDG 8 cut(s) 162, 189, 330, 566, 624, 663, 669, 713
Lsp1109I GCAGC 2 cut(s) 192, 676
LweI GCATC 2 cut(s) 427, 892
MaeI CTAG 2 cut(s) 927, 996
MalI GATC 2 cut(s) 282, 704
MboI GATC 2 cut(s) 280, 702
MfeI CAATTG 1 cut(s) 675
MhlI GDGCHC 2 cut(s) 97, 175
MlsI TGGCCA 1 cut(s) 917
MluNI TGGCCA 1 cut(s) 917
MlyI GAGTC 1 cut(s) 73
MmeI TCCRAC 2 cut(s) 515, 750
MnlI CCTC 6 cut(s) 48, 472, 540, 663, 892, 948
Mox20I TGGCCA 1 cut(s) 917
MroXI GAANNNNTTC 1 cut(s) 39
MscI TGGCCA 1 cut(s) 917
MseI TTAA 2 cut(s) 368, 825
MslI CAYNNNNRTG 1 cut(s) 78
Msp20I TGGCCA 1 cut(s) 917
MspR9I CCNGG 1 cut(s) 177
MunI CAATTG 1 cut(s) 675
MvaI CCWGG 1 cut(s) 177
MwoI GCNNNNNNNGC 1 cut(s) 992
NcoI CCATGG 1 cut(s) 918
NdeI CATATG 1 cut(s) 49
NdeII GATC 2 cut(s) 280, 702
NheI GCTAGC 1 cut(s) 995
NlaIII CATG 3 cut(s) 246, 922, 937
NlaIV GGNNCC 2 cut(s) 551, 609
NspV TTCGAA 1 cut(s) 892
PdmI GAANNNNTTC 1 cut(s) 39
PfeI GAWTC 1 cut(s) 614
PkrI GCNGC 3 cut(s) 182, 691, 1001
PleI GAGTC 1 cut(s) 72
PpsI GAGTC 1 cut(s) 72
PpuMI RGGWCCY 1 cut(s) 647
Psp5II RGGWCCY 1 cut(s) 647
Psp6I CCWGG 1 cut(s) 175
PspGI CCWGG 1 cut(s) 175
PspN4I GGNNCC 2 cut(s) 551, 609
PspPI GGNCC 1 cut(s) 647
PspPPI RGGWCCY 1 cut(s) 647
RsaI GTAC 3 cut(s) 522, 760, 1033
RsaNI GTAC 3 cut(s) 521, 759, 1032
RseI CAYNNNNRTG 1 cut(s) 78
SaqAI TTAA 2 cut(s) 368, 825
SatI GCNGC 3 cut(s) 181, 690, 1000
Sau3AI GATC 2 cut(s) 280, 702
Sau96I GGNCC 1 cut(s) 647
ScaI AGTACT 1 cut(s) 760
SchI GAGTC 1 cut(s) 73
ScrFI CCNGG 1 cut(s) 177
SduI GDGCHC 2 cut(s) 97, 175
SfaNI GCATC 2 cut(s) 427, 892
SfuI TTCGAA 1 cut(s) 892
SinI GGWCC 1 cut(s) 647
SmiMI CAYNNNNRTG 1 cut(s) 78
SpeI ACTAGT 1 cut(s) 926
SsiI CCGC 4 cut(s) 28, 815, 999, 1002
SspMI CTAG 2 cut(s) 927, 996
StyD4I CCNGG 1 cut(s) 175
StyI CCWWGG 1 cut(s) 918
TaaI ACNGT 4 cut(s) 17, 214, 377, 400
TaqI TCGA 3 cut(s) 62, 216, 892
TatI WGTACW 3 cut(s) 520, 758, 1031
TauI GCSGC 1 cut(s) 1002
TfiI GAWTC 1 cut(s) 614
Tru1I TTAA 2 cut(s) 368, 825
Tru9I TTAA 2 cut(s) 368, 825
TscAI CASTG 2 cut(s) 22, 403
TseI GCWGC 2 cut(s) 180, 689
TspDTI ATGAA 4 cut(s) 48, 138, 156, 585
TspGWI ACGGA 2 cut(s) 367, 473
TspRI CASTG 2 cut(s) 22, 403
VpaK11BI GGWCC 1 cut(s) 647
XagI CCTNNNNNAGG 1 cut(s) 667
XapI RAATTY 1 cut(s) 882
XmnI GAANNNNTTC 1 cut(s) 39
XspI CTAG 2 cut(s) 927, 996
ZrmI AGTACT 1 cut(s) 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.