FvH4_5g31251

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001050
Physical Location & Seq
Reverse (-)
22137910 .. 22139648
1739 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g31251.t1

Sequence Viewer

Length: 492 bp
ATGAATCCGTTCCAAGGTGACACACAAGCTACGTTCAACTCTGAAAGCGTTAGTAATGCACTTCAAGTTTCAAGTCAATATGGATCAAGCGGTGAATACAGAGCAACGCCAAATTATGGAGAGATTGGTGACTATGGAGGAATGGGTATTTATGAAGGACCTTTTGGATATGGAAAAGATAAAGACTATGGAGGAAGCGGAGACCCGCTTAAGTCGACCCGGGACTCGTTTCTATCTCACATAGAGCAAAACGTTGAGAGTTGCAGTTCAACCTCCCCTCGAAATTCTTCTATCTTCTTCTTCGCCATAACCAGTTCATCTACATTGAACCCAACAGGAGCCAATATAAACTACACAAGCTTCCCTCTTAACTTGAGATGCTATTTGTACTCCACTTCTGGGTGTTTCCAGTTCAACAAGCATACTCTTAATTGTCAACTCCTTTTCACATCCACGTATAAGCATACCGACTCTCACGGAGTGGCTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

164

Amino Acids

17.74

Weight (kDa)

5.42

Isoelectric Point (pI)

35.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 116
AccI GTMKAC 1 cut(s) 215
AciI CCGC 3 cut(s) 90, 198, 206
AclI AACGTT 1 cut(s) 252
AclWI GGATC 1 cut(s) 91
AcsI RAATTY 1 cut(s) 283
AdeI CACNNNGTG 1 cut(s) 481
AfaI GTAC 1 cut(s) 389
AfiI CCNNNNNNNGG 3 cut(s) 14, 116, 399
AflII CTTAAG 1 cut(s) 209
AgsI TTSAA 6 cut(s) 37, 65, 72, 270, 328, 415
AjuI GAANNNNNNNTTGG 2 cut(s) 147, 179
AluBI AGCT 2 cut(s) 29, 360
AluI AGCT 2 cut(s) 29, 360
Alw26I GTCTC 1 cut(s) 195
AlwI GGATC 1 cut(s) 91
Ama87I CYCGRG 1 cut(s) 219
ApoI RAATTY 1 cut(s) 283
Asp700I GAANNNNTTC 2 cut(s) 8, 286
AspS9I GGNCC 1 cut(s) 158
AsuC2I CCSGG 2 cut(s) 220, 221
AsuHPI GGTGA 3 cut(s) 29, 104, 140
AvaI CYCGRG 1 cut(s) 219
AvaII GGWCC 1 cut(s) 158
BcnI CCSGG 2 cut(s) 220, 221
BcoDI GTCTC 1 cut(s) 195
BfrI CTTAAG 1 cut(s) 209
Bme1390I CCNGG 2 cut(s) 220, 221
Bme18I GGWCC 1 cut(s) 158
BmeT110I CYCGRG 1 cut(s) 219
BmgT120I GGNCC 1 cut(s) 158
BmiI GGNNCC 1 cut(s) 340
BmrFI CCNGG 2 cut(s) 220, 221
BmsI GCATC 1 cut(s) 368
BpuEI CTTGAG 1 cut(s) 394
BpuMI CCSGG 2 cut(s) 220, 221
BsaAI YACGTR 1 cut(s) 456
BsaI GGTCTC 1 cut(s) 195
BsaJI CCNNGG 2 cut(s) 13, 219
Bsc4I CCNNNNNNNGG 3 cut(s) 14, 116, 399
Bse1I ACTGG 2 cut(s) 312, 409
BseDI CCNNGG 2 cut(s) 13, 219
BseGI GGATG 1 cut(s) 449
BseLI CCNNNNNNNGG 3 cut(s) 14, 116, 399
BseNI ACTGG 2 cut(s) 312, 409
BsiHKCI CYCGRG 1 cut(s) 219
BsiSI CCGG 1 cut(s) 220
BslFI GGGAC 1 cut(s) 236
BslI CCNNNNNNNGG 3 cut(s) 14, 116, 399
BsmAI GTCTC 1 cut(s) 195
BsmFI GGGAC 1 cut(s) 236
Bso31I GGTCTC 1 cut(s) 195
BsoBI CYCGRG 1 cut(s) 219
Bsp143I GATC 1 cut(s) 83
BspACI CCGC 3 cut(s) 90, 198, 206
BspLI GGNNCC 1 cut(s) 340
BspPI GGATC 1 cut(s) 91
BspTI CTTAAG 1 cut(s) 209
BspTNI GGTCTC 1 cut(s) 195
BsrI ACTGG 2 cut(s) 312, 409
BssECI CCNNGG 2 cut(s) 13, 219
BssMI GATC 1 cut(s) 83
BssT1I CCWWGG 1 cut(s) 13
BstAFI CTTAAG 1 cut(s) 209
BstBAI YACGTR 1 cut(s) 456
BstF5I GGATG 1 cut(s) 449
BstKTI GATC 1 cut(s) 86
BstMAI GTCTC 1 cut(s) 195
BstMBI GATC 1 cut(s) 83
BstSCI CCNGG 2 cut(s) 218, 219
BtsCI GGATG 1 cut(s) 449
Cfr13I GGNCC 1 cut(s) 158
Cfr9I CCCGGG 1 cut(s) 219
Csp6I GTAC 1 cut(s) 388
CviJI RGCY 4 cut(s) 29, 341, 360, 485
CviKI_1 RGCY 4 cut(s) 29, 341, 360, 485
CviQI GTAC 1 cut(s) 388
DpnI GATC 1 cut(s) 85
DpnII GATC 1 cut(s) 83
DraIII CACNNNGTG 1 cut(s) 481
Eco130I CCWWGG 1 cut(s) 13
Eco31I GGTCTC 1 cut(s) 195
Eco47I GGWCC 1 cut(s) 158
Eco88I CYCGRG 1 cut(s) 219
EcoO109I RGGNCCY 1 cut(s) 158
EcoT14I CCWWGG 1 cut(s) 13
ErhI CCWWGG 1 cut(s) 13
FaqI GGGAC 1 cut(s) 236
FauI CCCGC 1 cut(s) 213
FblI GTMKAC 1 cut(s) 215
FokI GGATG 1 cut(s) 436
HapII CCGG 1 cut(s) 220
HincII GTYRAC 2 cut(s) 216, 437
HindII GTYRAC 2 cut(s) 216, 437
HindIII AAGCTT 1 cut(s) 358
HinfI GANTC 3 cut(s) 4, 224, 470
HpaII CCGG 1 cut(s) 220
HphI GGTGA 3 cut(s) 29, 104, 140
Hpy166II GTNNAC 2 cut(s) 216, 437
Hpy188I TCNGA 1 cut(s) 43
Hpy8I GTNNAC 2 cut(s) 216, 437
HpyAV CCTTC 1 cut(s) 149
HpyCH4IV ACGT 3 cut(s) 32, 252, 455
HpyCH4V TGCA 2 cut(s) 59, 264
HpySE526I ACGT 3 cut(s) 32, 252, 455
Kzo9I GATC 1 cut(s) 83
LmnI GCTCC 1 cut(s) 338
LpnPI CCDG 5 cut(s) 233, 321, 325, 384, 422
LweI GCATC 1 cut(s) 368
MaeII ACGT 3 cut(s) 32, 252, 455
MaeIII GTNAC 2 cut(s) 17, 128
MalI GATC 1 cut(s) 85
MboI GATC 1 cut(s) 83
MboII GAAGA 4 cut(s) 279, 286, 289, 292
MluCI AATT 3 cut(s) 112, 283, 430
MlyI GAGTC 2 cut(s) 218, 464
MnlI CCTC 5 cut(s) 131, 185, 283, 288, 375
MroXI GAANNNNTTC 2 cut(s) 8, 286
MseI TTAA 4 cut(s) 210, 369, 429, 490
MspCI CTTAAG 1 cut(s) 209
MspI CCGG 1 cut(s) 220
MspR9I CCNGG 2 cut(s) 220, 221
NciI CCSGG 2 cut(s) 220, 221
NdeII GATC 1 cut(s) 83
NlaIV GGNNCC 1 cut(s) 340
NmuCI GTSAC 2 cut(s) 17, 128
PdmI GAANNNNTTC 2 cut(s) 8, 286
PfeI GAWTC 1 cut(s) 4
PflMI CCANNNNNTGG 1 cut(s) 116
PleI GAGTC 2 cut(s) 218, 464
PpsI GAGTC 2 cut(s) 218, 464
Ppu21I YACGTR 1 cut(s) 456
PpuMI RGGWCCY 1 cut(s) 158
Psp1406I AACGTT 1 cut(s) 252
Psp5II RGGWCCY 1 cut(s) 158
PspN4I GGNNCC 1 cut(s) 340
PspPI GGNCC 1 cut(s) 158
PspPPI RGGWCCY 1 cut(s) 158
RsaI GTAC 1 cut(s) 389
RsaNI GTAC 1 cut(s) 388
SalI GTCGAC 1 cut(s) 214
SaqAI TTAA 4 cut(s) 210, 369, 429, 490
Sau3AI GATC 1 cut(s) 83
Sau96I GGNCC 1 cut(s) 158
SchI GAGTC 2 cut(s) 218, 464
ScrFI CCNGG 2 cut(s) 220, 221
SetI ASST 8 cut(s) 19, 31, 35, 163, 255, 275, 362, 458
SfaNI GCATC 1 cut(s) 368
SinI GGWCC 1 cut(s) 158
SmaI CCCGGG 1 cut(s) 221
SmlI CTYRAG 2 cut(s) 209, 373
SmoI CTYRAG 2 cut(s) 209, 373
Sse9I AATT 3 cut(s) 112, 283, 430
SsiI CCGC 3 cut(s) 90, 198, 206
StyD4I CCNGG 2 cut(s) 218, 219
StyI CCWWGG 1 cut(s) 13
TaiI ACGT 3 cut(s) 35, 255, 458
TaqI TCGA 2 cut(s) 215, 280
TasI AATT 3 cut(s) 112, 283, 430
TatI WGTACW 1 cut(s) 387
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 4 cut(s) 210, 369, 429, 490
Tru9I TTAA 4 cut(s) 210, 369, 429, 490
TseFI GTSAC 2 cut(s) 17, 128
Tsp45I GTSAC 2 cut(s) 17, 128
TspDTI ATGAA 3 cut(s) 17, 168, 306
TspGWI ACGGA 1 cut(s) 492
TspMI CCCGGG 1 cut(s) 219
Van91I CCANNNNNTGG 1 cut(s) 116
Vha464I CTTAAG 1 cut(s) 209
VpaK11BI GGWCC 1 cut(s) 158
XapI RAATTY 1 cut(s) 283
XmaI CCCGGG 1 cut(s) 219
XmiI GTMKAC 1 cut(s) 215
XmnI GAANNNNTTC 2 cut(s) 8, 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.