Rroxscaffold_3G00259610

No apical meristem-associated C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001050
Physical Location & Seq
Forward (+)
54032868 .. 54045225
12358 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00259610.1

Sequence Viewer

Length: 441 bp
ATGCGGGTTGCTCCTCTCAATCAAATTGTCAAGGCTCTTTGTATATTGACTCGTCGGATAGTAGAAGCAAAAGAGAATTTCAAGCTAGAGGAAGGTCATGCATTTGTACTTGACCATTGTTGGGTTGTTTTGAGGTTTCAGCAAAAATGGATGGATGAACATCAAAAAATCGAAAGTAAGAGAAAGTGGAAGGCTCCTTCTAGTAAGGCTGCAGATTCTATCAATTTAGAAGACGATGATACGGAAGCTCCCGAACCTGCAAGCTTGAAGAGACCAATTGGGAGAAAGGCTGCAAAGGAAGCTATCAAAAAGGCAAAATCCAAAGAACAGATTGGGGATGTTCAGGGCCAGTCGGGAGATTCTAACGAGGTACGTATTATGGCGGCACACACCGGTTGGAGACCAACGTCGACGTTGGTCACAACGGATGACGGCGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM-associated

Protein Analysis

146

Amino Acids

16.4

Weight (kDa)

8.89

Isoelectric Point (pI)

44.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM-associated PF14303 34 - 121 2.3e-13 No apical meristem-associated C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 265
AccI GTMKAC 1 cut(s) 410
AciI CCGC 2 cut(s) 4, 383
AcsI RAATTY 1 cut(s) 76
AfaI GTAC 2 cut(s) 108, 372
AfiI CCNNNNNNNGG 1 cut(s) 121
AgeI ACCGGT 1 cut(s) 392
AgsI TTSAA 2 cut(s) 82, 268
AluBI AGCT 4 cut(s) 85, 248, 264, 302
AluI AGCT 4 cut(s) 85, 248, 264, 302
Alw26I GTCTC 2 cut(s) 265, 394
AoxI GGCC 1 cut(s) 346
ApeKI GCWGC 2 cut(s) 209, 290
ApoI RAATTY 1 cut(s) 76
AsiGI ACCGGT 1 cut(s) 392
AspLEI GCGC 1 cut(s) 437
AspS9I GGNCC 1 cut(s) 346
BbsI GAAGAC 1 cut(s) 237
BbvI GCAGC 2 cut(s) 196, 277
BccI CCATC 1 cut(s) 145
BcoDI GTCTC 2 cut(s) 265, 394
BfaI CTAG 2 cut(s) 86, 201
BfmI CTRYAG 1 cut(s) 210
BfoI RGCGCY 1 cut(s) 438
BfuAI ACCTGC 1 cut(s) 265
BisI GCNGC 3 cut(s) 210, 291, 384
BlsI GCNGC 3 cut(s) 211, 292, 385
BmgT120I GGNCC 1 cut(s) 346
BmiI GGNNCC 1 cut(s) 195
BoxI GACNNNNGTC 2 cut(s) 406, 416
BpiI GAAGAC 1 cut(s) 237
BsaAI YACGTR 1 cut(s) 374
BsaBI GATNNNNATC 1 cut(s) 159
BsaI GGTCTC 2 cut(s) 265, 394
BsaWI WCCGGW 1 cut(s) 392
BsaXI ACNNNNNCTCC 2 cut(s) 232, 262
Bsc4I CCNNNNNNNGG 1 cut(s) 121
Bse118I RCCGGY 1 cut(s) 392
Bse1I ACTGG 1 cut(s) 349
Bse8I GATNNNNATC 1 cut(s) 159
BseGI GGATG 4 cut(s) 156, 160, 343, 433
BseJI GATNNNNATC 1 cut(s) 159
BseLI CCNNNNNNNGG 1 cut(s) 121
BseNI ACTGG 1 cut(s) 349
BseXI GCAGC 2 cut(s) 196, 277
BshFI GGCC 1 cut(s) 348
BshTI ACCGGT 1 cut(s) 392
BsiSI CCGG 1 cut(s) 393
BslI CCNNNNNNNGG 1 cut(s) 121
BsmAI GTCTC 2 cut(s) 265, 394
BsnI GGCC 1 cut(s) 348
Bso31I GGTCTC 2 cut(s) 265, 394
BspACI CCGC 2 cut(s) 4, 383
BspANI GGCC 1 cut(s) 348
BspLI GGNNCC 1 cut(s) 195
BspMAI CTGCAG 1 cut(s) 214
BspMI ACCTGC 1 cut(s) 265
BspTNI GGTCTC 2 cut(s) 265, 394
BsrFI RCCGGY 1 cut(s) 392
BsrI ACTGG 1 cut(s) 349
BssAI RCCGGY 1 cut(s) 392
Bst6I CTCTTC 1 cut(s) 263
BstBAI YACGTR 1 cut(s) 374
BstC8I GCNNGC 1 cut(s) 262
BstDEI CTNAG 1 cut(s) 438
BstF5I GGATG 4 cut(s) 156, 160, 343, 433
BstH2I RGCGCY 1 cut(s) 438
BstHHI GCGC 1 cut(s) 437
BstMAI GTCTC 2 cut(s) 265, 394
BstMWI GCNNNNNNNGC 1 cut(s) 299
BstPAI GACNNNNGTC 2 cut(s) 406, 416
BstSFI CTRYAG 1 cut(s) 210
BstSNI TACGTA 1 cut(s) 374
BstV1I GCAGC 2 cut(s) 196, 277
BstV2I GAAGAC 1 cut(s) 237
BsuRI GGCC 1 cut(s) 348
BtsCI GGATG 4 cut(s) 156, 160, 343, 433
BveI ACCTGC 1 cut(s) 265
Cac8I GCNNGC 1 cut(s) 262
CfoI GCGC 1 cut(s) 437
Cfr10I RCCGGY 1 cut(s) 392
Cfr13I GGNCC 1 cut(s) 346
Csp6I GTAC 2 cut(s) 107, 371
CspAI ACCGGT 1 cut(s) 392
CviAII CATG 1 cut(s) 98
CviJI RGCY 9 cut(s) 35, 85, 194, 209, 248, 264, 290, 302, 348
CviKI_1 RGCY 9 cut(s) 35, 85, 194, 209, 248, 264, 290, 302, 348
CviQI GTAC 2 cut(s) 107, 371
DdeI CTNAG 1 cut(s) 438
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
Eco105I TACGTA 1 cut(s) 374
Eco31I GGTCTC 2 cut(s) 265, 394
EcoT22I ATGCAT 1 cut(s) 103
FaeI CATG 1 cut(s) 101
FaiI YATR 3 cut(s) 44, 99, 380
FatI CATG 1 cut(s) 97
FblI GTMKAC 1 cut(s) 410
Fnu4HI GCNGC 3 cut(s) 210, 291, 384
FokI GGATG 3 cut(s) 163, 167, 350
Fsp4HI GCNGC 3 cut(s) 210, 291, 384
FspBI CTAG 2 cut(s) 86, 201
GlaI GCGC 1 cut(s) 436
GluI GCNGC 3 cut(s) 210, 291, 384
HaeII RGCGCY 1 cut(s) 438
HaeIII GGCC 1 cut(s) 348
HapII CCGG 1 cut(s) 393
HhaI GCGC 1 cut(s) 437
Hin1II CATG 1 cut(s) 101
Hin6I GCGC 1 cut(s) 435
HinP1I GCGC 1 cut(s) 435
HincII GTYRAC 1 cut(s) 411
HindII GTYRAC 1 cut(s) 411
HindIII AAGCTT 1 cut(s) 262
HinfI GANTC 3 cut(s) 49, 215, 359
HpaII CCGG 1 cut(s) 393
Hpy166II GTNNAC 1 cut(s) 411
Hpy188I TCNGA 1 cut(s) 57
Hpy188III TCNNGA 2 cut(s) 251, 354
Hpy8I GTNNAC 1 cut(s) 411
Hpy99I CGWCG 3 cut(s) 57, 412, 415
HpyAV CCTTC 3 cut(s) 86, 184, 207
HpyCH4IV ACGT 3 cut(s) 373, 407, 413
HpyCH4V TGCA 4 cut(s) 101, 212, 260, 293
HpyF10VI GCNNNNNNNGC 1 cut(s) 299
HpyF3I CTNAG 1 cut(s) 438
HpySE526I ACGT 3 cut(s) 373, 407, 413
Hsp92II CATG 1 cut(s) 101
HspAI GCGC 1 cut(s) 435
LmnI GCTCC 3 cut(s) 16, 199, 253
LpnPI CCDG 4 cut(s) 270, 329, 362, 406
Lsp1109I GCAGC 2 cut(s) 196, 277
MaeI CTAG 2 cut(s) 86, 201
MaeII ACGT 3 cut(s) 373, 407, 413
MaeIII GTNAC 1 cut(s) 418
MboII GAAGA 2 cut(s) 242, 280
MfeI CAATTG 1 cut(s) 276
MluCI AATT 4 cut(s) 24, 76, 223, 276
MlyI GAGTC 1 cut(s) 43
MmeI TCCRAC 2 cut(s) 35, 377
MnlI CCTC 4 cut(s) 24, 82, 126, 361
Mph1103I ATGCAT 1 cut(s) 103
MspI CCGG 1 cut(s) 393
MunI CAATTG 1 cut(s) 276
MwoI GCNNNNNNNGC 1 cut(s) 299
NlaIII CATG 1 cut(s) 101
NlaIV GGNNCC 1 cut(s) 195
NmuCI GTSAC 1 cut(s) 418
NsiI ATGCAT 1 cut(s) 103
PfeI GAWTC 2 cut(s) 215, 359
PinAI ACCGGT 1 cut(s) 392
PkrI GCNGC 3 cut(s) 211, 292, 385
PleI GAGTC 1 cut(s) 43
PpsI GAGTC 1 cut(s) 43
Ppu21I YACGTR 1 cut(s) 374
PshAI GACNNNNGTC 2 cut(s) 406, 416
PspN4I GGNNCC 1 cut(s) 195
PspPI GGNCC 1 cut(s) 346
PstI CTGCAG 1 cut(s) 214
RsaI GTAC 2 cut(s) 108, 372
RsaNI GTAC 2 cut(s) 107, 371
SalI GTCGAC 1 cut(s) 409
SatI GCNGC 3 cut(s) 210, 291, 384
Sau96I GGNCC 1 cut(s) 346
SchI GAGTC 1 cut(s) 43
SfcI CTRYAG 1 cut(s) 210
SgrDI CGTCGACG 1 cut(s) 409
SnaBI TACGTA 1 cut(s) 374
Sse9I AATT 4 cut(s) 24, 76, 223, 276
SsiI CCGC 2 cut(s) 4, 383
SspMI CTAG 2 cut(s) 86, 201
TaiI ACGT 3 cut(s) 376, 410, 416
TaqI TCGA 2 cut(s) 171, 410
TasI AATT 4 cut(s) 24, 76, 223, 276
TatI WGTACW 1 cut(s) 106
TauI GCSGC 1 cut(s) 386
TfiI GAWTC 2 cut(s) 215, 359
TseFI GTSAC 1 cut(s) 418
TseI GCWGC 2 cut(s) 209, 290
Tsp45I GTSAC 1 cut(s) 418
TspDTI ATGAA 1 cut(s) 171
TspGWI ACGGA 2 cut(s) 257, 440
XapI RAATTY 1 cut(s) 76
XmiI GTMKAC 1 cut(s) 410
XspI CTAG 2 cut(s) 86, 201
Zsp2I ATGCAT 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.