FvH4_4g24710

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000622
Physical Location & Seq
Reverse (-)
26754087 .. 26754650
564 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g24710.t1

Sequence Viewer

Length: 564 bp
ATGGCCAATACCATCAAGATTACAGGAGCTCATGATGAGAAGTTGTATATTGTGAGCCATGCTGTGAACCTACGCTCAAAGTTTGATCCACCGTTGCCAAAACATTCTTTTGGAAACATTTACCGTGTTGCCATGACAGGTCCCTTGTTAAGTACTGGGGATGCATGCAATGCATATGGTTGGGCTATGAGGGAAGTACGAGAAGAGATAAACAAGGTCGACAATGAGTGCACCAATGGATTACAGCAAGGCGGTGAACAGTCAGGGCTCCCCATGAATGAGAATGCTGCTAGACTAATGGAACTAGAGGTGGTTGCGTTGGGCTTCAGTAGCTATTGCAGGTTTCCAGCGTATGATAATGATTTCGGTTGGGGAAGGCCTGCGTGGGTGTTCTCAGCACCATTGACCTTCAAGAACATAATAGCTTTCATGGGCACCAAAGAGGGCGACGGAATAGAGGCATACATCAGCTTGGAGGAGCAACTCATGGCTGAATTTGAAACTGATGCCGAGTTCTTGGCCTATGTGTCTCCAAAGGGTGCTGAAGAGTCCTTTGGTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

188

Amino Acids

20.67

Weight (kDa)

4.69

Isoelectric Point (pI)

44.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 18 - 172 1e-22 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24710 FvH4_4g24720 FvH4_4g24770 FvH4_4g24771 FvH4_4g24772 FvH4_5g39380
malus_domestica MD10G1199500.v1.1 MD13G1109100.v1.1 MD13G1109200.v1.1 MD13G1114400.v1.1 MD13G1114800.v1.1 MD16G1108800.v1.1
prunus_persica Prupe.1G235900_v2.0.a1 Prupe.1G236600_v2.0.a1 Prupe.1G236700_v2.0.a1 Prupe.1G236800_v2.0.a1 Prupe.4G142000_v2.0.a1
pyrus_communis pycom10g17180 pycom13g09460 pycom13g09930 pycom13g09940
rosa_chinensis RchiOBHm_Chr3g0490281 RchiOBHm_Chr3g0490291 RchiOBHm_Chr3g0490301 RchiOBHm_Chr4g0417441 RchiOBHm_Chr4g0417461 RchiOBHm_Chr4g0431811 RchiOBHm_Chr4g0431821 RchiOBHm_Chr4g0431851
rosa_laevigata RLG00000006882 RLG00000006886 RLG00000006887 RLG00000007905 RLG00000007911 RLG00000007913 RLG00000029538
rosa_multiflora Rmu_sc0001459.1_g000008 Rmu_sc0001459.1_g000011 Rmu_sc0002489.1_g000020 Rmu_sc0002489.1_g000024 Rmu_sc0002489.1_g000027 Rmu_sc0002489.1_g000028 Rmu_sc0002489.1_g000029 Rmu_sc0004142.1_g000001 Rmu_sc0004142.1_g000024 Rmu_sc0011237.1_g000018 Rmu_sc0031439.1_g000001 Rmu_sc0031677.1_g000001 Rmu_ssc0000361.1_g000005
rosa_roxburghii Rroxscaffold_3G00262760 Rroxscaffold_4G00317310 Rroxscaffold_5G00361150 Rroxscaffold_5G00373190 Rroxscaffold_5G00373210 Rroxscaffold_5G00373220 Rroxscaffold_5G00373240
rosa_rugosa Rorug01G0111100 Rorug04G0252400 Rorug04G0252400 Rorug04G0252400 Rorug04G0252600
rosa_samantha Rh1BG102300 Rh3AG292200 Rh3AG292300 Rh4AG309100 Rh4AG309300 Rh4AG309500 Rh6BG200900
rosa_wichuraiana Rw0G002990 Rw0G003000 Rw0G019170 Rw3G025890 Rw3G025900 Rw4G017830 Rw4G026810 Rw4G026870 Rw4G026880 Rw4G026920 Rw6G017190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 330
AccB1I GGYRCC 1 cut(s) 434
AccI GTMKAC 1 cut(s) 219
AciI CCGC 1 cut(s) 252
AclWI GGATC 1 cut(s) 80
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 494
AcuI CTGAAG 2 cut(s) 310, 564
AfaI GTAC 2 cut(s) 154, 198
AgsI TTSAA 2 cut(s) 412, 500
AjuI GAANNNNNNNTTGG 1 cut(s) 537
AluBI AGCT 4 cut(s) 29, 333, 425, 471
AluI AGCT 4 cut(s) 29, 333, 425, 471
Alw21I GWGCWC 2 cut(s) 31, 233
Alw26I GTCTC 1 cut(s) 534
Alw44I GTGCAC 1 cut(s) 229
AlwI GGATC 1 cut(s) 80
AoxI GGCC 3 cut(s) 3, 377, 519
ApaLI GTGCAC 1 cut(s) 229
ApeKI GCWGC 1 cut(s) 287
ApoI RAATTY 1 cut(s) 494
AspS9I GGNCC 1 cut(s) 140
AsuHPI GGTGA 1 cut(s) 266
AvaII GGWCC 1 cut(s) 140
BaeGI GKGCMC 2 cut(s) 233, 437
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 434
BanII GRGCYC 2 cut(s) 31, 270
Bbv12I GWGCWC 2 cut(s) 31, 233
BbvI GCAGC 1 cut(s) 274
BccI CCATC 1 cut(s) 20
BcoDI GTCTC 1 cut(s) 534
BfaI CTAG 2 cut(s) 291, 305
BfuAI ACCTGC 1 cut(s) 330
BisI GCNGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 289
BmcAI AGTACT 1 cut(s) 154
Bme18I GGWCC 1 cut(s) 140
BmgT120I GGNCC 1 cut(s) 140
BmiI GGNNCC 3 cut(s) 142, 269, 436
BmrI ACTGGG 1 cut(s) 165
BmsI GCATC 2 cut(s) 151, 496
BmuI ACTGGG 1 cut(s) 165
Bse1I ACTGG 1 cut(s) 160
Bse3DI GCAATG 1 cut(s) 175
BseGI GGATG 1 cut(s) 166
BseMI GCAATG 1 cut(s) 175
BseMII CTCAG 1 cut(s) 408
BseNI ACTGG 1 cut(s) 160
BseRI GAGGAG 1 cut(s) 491
BseSI GKGCMC 2 cut(s) 233, 437
BseXI GCAGC 1 cut(s) 274
BshFI GGCC 3 cut(s) 5, 379, 521
BshNI GGYRCC 1 cut(s) 434
BsiHKAI GWGCWC 2 cut(s) 31, 233
BslFI GGGAC 1 cut(s) 126
BsmAI GTCTC 1 cut(s) 534
BsmFI GGGAC 1 cut(s) 126
BsmI GAATGC 1 cut(s) 289
BsnI GGCC 3 cut(s) 5, 379, 521
Bsp1286I GDGCHC 4 cut(s) 31, 233, 270, 437
Bsp143I GATC 1 cut(s) 85
BspACI CCGC 1 cut(s) 252
BspANI GGCC 3 cut(s) 5, 379, 521
BspCNI CTCAG 1 cut(s) 407
BspHI TCATGA 1 cut(s) 31
BspLI GGNNCC 3 cut(s) 142, 269, 436
BspMI ACCTGC 1 cut(s) 330
BspPI GGATC 1 cut(s) 80
BspT107I GGYRCC 1 cut(s) 434
BsrDI GCAATG 1 cut(s) 175
BsrI ACTGG 1 cut(s) 160
BssMI GATC 1 cut(s) 85
Bst4CI ACNGT 3 cut(s) 93, 125, 261
Bst6I CTCTTC 2 cut(s) 198, 540
BstAPI GCANNNNNTGC 1 cut(s) 170
BstC8I GCNNGC 2 cut(s) 166, 381
BstDEI CTNAG 1 cut(s) 394
BstF5I GGATG 1 cut(s) 166
BstKTI GATC 1 cut(s) 88
BstMAI GTCTC 1 cut(s) 534
BstMBI GATC 1 cut(s) 85
BstMWI GCNNNNNNNGC 2 cut(s) 170, 330
BstNSI RCATGY 1 cut(s) 168
BstSLI GKGCMC 2 cut(s) 233, 437
BstV1I GCAGC 1 cut(s) 274
BsuRI GGCC 3 cut(s) 5, 379, 521
BtsCI GGATG 1 cut(s) 166
BveI ACCTGC 1 cut(s) 330
Cac8I GCNNGC 2 cut(s) 166, 381
CciI TCATGA 1 cut(s) 31
Cfr13I GGNCC 1 cut(s) 140
Csp6I GTAC 2 cut(s) 153, 197
CviAII CATG 7 cut(s) 32, 59, 133, 165, 274, 430, 487
CviQI GTAC 2 cut(s) 153, 197
DdeI CTNAG 1 cut(s) 394
DpnI GATC 1 cut(s) 87
DpnII GATC 1 cut(s) 85
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 198, 540
EarI CTCTTC 2 cut(s) 198, 540
Ecl136II GAGCTC 1 cut(s) 29
Eco147I AGGCCT 1 cut(s) 379
Eco24I GRGCYC 2 cut(s) 31, 270
Eco47I GGWCC 1 cut(s) 140
Eco53kI GAGCTC 1 cut(s) 29
Eco57I CTGAAG 2 cut(s) 310, 564
EcoICRI GAGCTC 1 cut(s) 29
EcoO109I RGGNCCY 1 cut(s) 140
EcoT22I ATGCAT 2 cut(s) 166, 175
EcoT38I GRGCYC 2 cut(s) 31, 270
FaeI CATG 7 cut(s) 35, 62, 136, 168, 277, 433, 490
FaqI GGGAC 1 cut(s) 126
FatI CATG 7 cut(s) 31, 58, 132, 164, 273, 429, 486
FauNDI CATATG 1 cut(s) 175
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 1 cut(s) 288
FokI GGATG 1 cut(s) 173
FriOI GRGCYC 2 cut(s) 31, 270
Fsp4HI GCNGC 1 cut(s) 288
FspBI CTAG 2 cut(s) 291, 305
GluI GCNGC 1 cut(s) 288
HaeIII GGCC 3 cut(s) 5, 379, 521
Hin1II CATG 7 cut(s) 35, 62, 136, 168, 277, 433, 490
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HinfI GANTC 1 cut(s) 548
HphI GGTGA 1 cut(s) 266
Hpy166II GTNNAC 4 cut(s) 67, 220, 231, 257
Hpy188III TCNNGA 3 cut(s) 16, 32, 412
Hpy8I GTNNAC 4 cut(s) 67, 220, 231, 257
Hpy99I CGWCG 1 cut(s) 452
HpyAV CCTTC 2 cut(s) 369, 418
HpyCH4III ACNGT 3 cut(s) 93, 125, 261
HpyCH4V TGCA 5 cut(s) 164, 168, 173, 231, 339
HpyF10VI GCNNNNNNNGC 2 cut(s) 170, 330
HpyF3I CTNAG 1 cut(s) 394
Hsp92II CATG 7 cut(s) 35, 62, 136, 168, 277, 433, 490
Kzo9I GATC 1 cut(s) 85
LmnI GCTCC 3 cut(s) 26, 273, 478
LpnPI CCDG 7 cut(s) 9, 123, 141, 249, 325, 360, 393
Lsp1109I GCAGC 1 cut(s) 274
LweI GCATC 2 cut(s) 151, 496
MaeI CTAG 2 cut(s) 291, 305
MalI GATC 1 cut(s) 87
MboI GATC 1 cut(s) 85
MboII GAAGA 2 cut(s) 215, 557
MhlI GDGCHC 4 cut(s) 31, 233, 270, 437
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 494
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 557
MnlI CCTC 5 cut(s) 183, 301, 436, 451, 469
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 2 cut(s) 166, 175
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 149, 562
Msp20I TGGCCA 1 cut(s) 5
Mva1269I GAATGC 1 cut(s) 289
MwoI GCNNNNNNNGC 2 cut(s) 170, 330
NdeI CATATG 1 cut(s) 175
NdeII GATC 1 cut(s) 85
NlaIII CATG 7 cut(s) 35, 62, 136, 168, 277, 433, 490
NlaIV GGNNCC 3 cut(s) 142, 269, 436
NmeAIII GCCGAG 1 cut(s) 535
NsiI ATGCAT 2 cut(s) 166, 175
NspI RCATGY 1 cut(s) 168
PaeI GCATGC 1 cut(s) 168
PagI TCATGA 1 cut(s) 31
PceI AGGCCT 1 cut(s) 379
PctI GAATGC 1 cut(s) 289
PkrI GCNGC 1 cut(s) 289
PleI GAGTC 1 cut(s) 556
PpsI GAGTC 1 cut(s) 556
PpuMI RGGWCCY 1 cut(s) 140
Psp124BI GAGCTC 1 cut(s) 31
Psp5II RGGWCCY 1 cut(s) 140
PspN4I GGNNCC 3 cut(s) 142, 269, 436
PspPI GGNCC 1 cut(s) 140
PspPPI RGGWCCY 1 cut(s) 140
RsaI GTAC 2 cut(s) 154, 198
RsaNI GTAC 2 cut(s) 153, 197
SacI GAGCTC 1 cut(s) 31
SalI GTCGAC 1 cut(s) 218
SaqAI TTAA 2 cut(s) 149, 562
SatI GCNGC 1 cut(s) 288
Sau3AI GATC 1 cut(s) 85
Sau96I GGNCC 1 cut(s) 140
ScaI AGTACT 1 cut(s) 154
SchI GAGTC 1 cut(s) 557
SduI GDGCHC 4 cut(s) 31, 233, 270, 437
SfaNI GCATC 2 cut(s) 151, 496
SinI GGWCC 1 cut(s) 140
SphI GCATGC 1 cut(s) 168
Sse9I AATT 1 cut(s) 494
SseBI AGGCCT 1 cut(s) 379
SsiI CCGC 1 cut(s) 252
SspMI CTAG 2 cut(s) 291, 305
SstI GAGCTC 1 cut(s) 31
StuI AGGCCT 1 cut(s) 379
TaaI ACNGT 3 cut(s) 93, 125, 261
TaqI TCGA 1 cut(s) 219
TasI AATT 1 cut(s) 494
TatI WGTACW 1 cut(s) 152
Tru1I TTAA 2 cut(s) 149, 562
Tru9I TTAA 2 cut(s) 149, 562
TseI GCWGC 1 cut(s) 287
TspDTI ATGAA 2 cut(s) 290, 418
TspGWI ACGGA 1 cut(s) 465
VneI GTGCAC 1 cut(s) 229
VpaK11BI GGWCC 1 cut(s) 140
XapI RAATTY 1 cut(s) 494
XceI RCATGY 1 cut(s) 168
XmiI GTMKAC 1 cut(s) 219
XspI CTAG 2 cut(s) 291, 305
ZrmI AGTACT 1 cut(s) 154
Zsp2I ATGCAT 2 cut(s) 166, 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.