Rroxscaffold_5G00373240

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000622
Physical Location & Seq
Reverse (-)
54495194 .. 54496964
1771 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00373240.1

Sequence Viewer

Length: 1272 bp
ATGAAGTTTGAAGCTGAAGTAATCTCCAAGCAGATTATCACGCCTTCTTCTCCAACCCCTGACCATCTTCGCCATTATCAGTTCTCATTTCTTGATCAAATATCTCCCCCGGTCTATAACCCTTTGCTTCTCTTTTATGAATTCAATGCCAAAACTCAACCCAACATCACTGAAATATCCAACCACCTGAAGACCTCCTTGGCCAAAGTCTTAACCCTTTTCTACCCCCTAGCCGGACGACTCAAAGGCAACCAATTCGTGGATTGCAACGACATTGGTATACCCTACCTTGAAGCTAAAGTGAACTGCAAACTCTCAGATGTTCTCGACAATCCAGTACCCGGAGAACTCAACAAGCTCATGCCATTTGAGCTGGACGACGTGGCAAACAAATCTGCCCTTGGCGTCCAGCTAAACATTTTTGAATGTGGTGGTTTTGCTATTGCTCAATGCCTTTCTCACAAGCTCGCAGATGGGTTATCATACTTCACGTTCAGCAAAATTTGGGCCGCCACTGCCCGTGGTGAACCCAAAATAGCACATCCACAGTTCATTTCCGCCACACTATTCCCTCCCAAGAATTTAAGTGGATATGATGCGCGCACTGGTATCACAAGAAACAAAGTCACAAAAAGATTTGTGTTTAGTGCCTCTAACATAGAGGAGCTCAGAAAAGATCAAGCACATAAGGCCTTAACACGCCCTTCACGTGTTGAGGCCTTATCTTCTTTCATCTTGAGCCGATTTGTGGAGGCTACCAAAGACATTGGATCAGCTGACAAGTTTTACAGGGTGGTTCATGCTGTGAACTTGCGGCCAAGGTTCGACCCACCACTGCCGCACCACTCCTTTGGGAACCTTTACCGAGTAGCCATGACAGCTCCGATACAAATAAGTTCCCGAGAACCATATCATGGTATACGAGAAGTGATAAGCAAGATCGACAATGAGTACGTAAGAAAACTACAGAAAGGTGATGAGCATTTGAGTTCCATCAAGACCTATGCTAAAAGTTTTGCTAAAGGCGAGCAGGTTACAATTAGCTTCAGTAGTTATTGCAGGTTTCCTCTGTACGATAACGATTTTGGTTGGGGGAGGCCTACGTGGGTGGGGTCGCCGGCACTGACGTTCAAGAACCTAGTGAACTTCATGGATACCAAAGAGGGTGGTGGGATTGAGGCATATATTAGCTTGGAGGAGGAAGTCATGACTAAGTTTGAGAGCGATGTGGAGCTTTTAGCTTATGTGTCTCCAAGTGCGGTGCTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

423

Amino Acids

47.52

Weight (kDa)

8.39

Isoelectric Point (pI)

41.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 412 2.9e-76 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24710 FvH4_4g24720 FvH4_4g24770 FvH4_4g24771 FvH4_4g24772 FvH4_5g39380
malus_domestica MD10G1199500.v1.1 MD13G1109100.v1.1 MD13G1109200.v1.1 MD13G1114400.v1.1 MD13G1114800.v1.1 MD16G1108800.v1.1
prunus_persica Prupe.1G235900_v2.0.a1 Prupe.1G236600_v2.0.a1 Prupe.1G236700_v2.0.a1 Prupe.1G236800_v2.0.a1 Prupe.4G142000_v2.0.a1
pyrus_communis pycom10g17180 pycom13g09460 pycom13g09930 pycom13g09940
rosa_chinensis RchiOBHm_Chr3g0490281 RchiOBHm_Chr3g0490291 RchiOBHm_Chr3g0490301 RchiOBHm_Chr4g0417441 RchiOBHm_Chr4g0417461 RchiOBHm_Chr4g0431811 RchiOBHm_Chr4g0431821 RchiOBHm_Chr4g0431851
rosa_laevigata RLG00000006882 RLG00000006886 RLG00000006887 RLG00000007905 RLG00000007911 RLG00000007913 RLG00000029538
rosa_multiflora Rmu_sc0001459.1_g000008 Rmu_sc0001459.1_g000011 Rmu_sc0002489.1_g000020 Rmu_sc0002489.1_g000024 Rmu_sc0002489.1_g000027 Rmu_sc0002489.1_g000028 Rmu_sc0002489.1_g000029 Rmu_sc0004142.1_g000001 Rmu_sc0004142.1_g000024 Rmu_sc0011237.1_g000018 Rmu_sc0031439.1_g000001 Rmu_sc0031677.1_g000001 Rmu_ssc0000361.1_g000005
rosa_roxburghii Rroxscaffold_3G00262760 Rroxscaffold_4G00317310 Rroxscaffold_5G00361150 Rroxscaffold_5G00373190 Rroxscaffold_5G00373210 Rroxscaffold_5G00373220 Rroxscaffold_5G00373240
rosa_rugosa Rorug01G0111100 Rorug04G0252400 Rorug04G0252400 Rorug04G0252400 Rorug04G0252600
rosa_samantha Rh1BG102300 Rh3AG292200 Rh3AG292300 Rh4AG309100 Rh4AG309300 Rh4AG309500 Rh6BG200900
rosa_wichuraiana Rw0G002990 Rw0G003000 Rw0G019170 Rw3G025890 Rw3G025900 Rw4G017830 Rw4G026810 Rw4G026870 Rw4G026880 Rw4G026920 Rw6G017190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1021, 1050
AccB7I CCANNNNNTGG 2 cut(s) 259, 914
AccI GTMKAC 2 cut(s) 280, 919
AccII CGCG 1 cut(s) 601
AciI CCGC 5 cut(s) 510, 558, 814, 839, 1259
AclWI GGATC 1 cut(s) 778
AcoI YGGCCR 2 cut(s) 201, 815
AcsI RAATTY 3 cut(s) 140, 501, 580
AcuI CTGAAG 3 cut(s) 36, 209, 1030
AcvI CACGTG 1 cut(s) 710
AcyI GRCGYC 1 cut(s) 405
AfaI GTAC 3 cut(s) 339, 953, 1073
AfiI CCNNNNNNNGG 5 cut(s) 233, 259, 341, 748, 914
AflIII ACRYGT 1 cut(s) 709
AgsI TTSAA 5 cut(s) 11, 145, 293, 425, 1132
AjiI CACGTC 1 cut(s) 382
AjuI GAANNNNNNNTTGG 2 cut(s) 182, 214
Alw21I GWGCWC 1 cut(s) 669
Alw26I GTCTC 1 cut(s) 1254
AlwI GGATC 1 cut(s) 778
Ama87I CYCGRG 1 cut(s) 900
AoxI GGCC 6 cut(s) 201, 507, 690, 717, 815, 1097
ApoI RAATTY 3 cut(s) 140, 501, 580
AspLEI GCGC 2 cut(s) 601, 603
AspS9I GGNCC 1 cut(s) 507
AsuC2I CCSGG 2 cut(s) 110, 342
AsuHPI GGTGA 2 cut(s) 536, 986
AvaI CYCGRG 1 cut(s) 900
BalI TGGCCA 1 cut(s) 203
BanII GRGCYC 1 cut(s) 669
BbrPI CACGTG 1 cut(s) 710
BbsI GAAGAC 1 cut(s) 197
Bbv12I GWGCWC 1 cut(s) 669
BccI CCATC 3 cut(s) 72, 467, 1001
BcgI CGANNNNNNTGC 2 cut(s) 238, 272
BciVI GTATCC 1 cut(s) 1147
BclI TGATCA 1 cut(s) 94
BcnI CCSGG 2 cut(s) 110, 342
BcoDI GTCTC 1 cut(s) 1254
BfaI CTAG 2 cut(s) 230, 1139
BfmI CTRYAG 1 cut(s) 965
BfuAI ACCTGC 2 cut(s) 1021, 1050
BfuI GTATCC 1 cut(s) 1147
BisI GCNGC 3 cut(s) 510, 815, 839
BlsI GCNGC 3 cut(s) 511, 816, 840
Bme1390I CCNGG 2 cut(s) 110, 342
BmeT110I CYCGRG 1 cut(s) 900
BmgBI CACGTC 1 cut(s) 382
BmgT120I GGNCC 1 cut(s) 507
BmiI GGNNCC 1 cut(s) 857
BmrFI CCNGG 2 cut(s) 110, 342
BmsI GCATC 1 cut(s) 586
BpiI GAAGAC 1 cut(s) 197
BpuEI CTTGAG 1 cut(s) 757
BpuMI CCSGG 2 cut(s) 110, 342
BsaAI YACGTR 3 cut(s) 710, 955, 1104
BsaHI GRCGYC 1 cut(s) 405
BsaJI CCNNGG 5 cut(s) 108, 198, 400, 520, 818
Bsc4I CCNNNNNNNGG 5 cut(s) 233, 259, 341, 748, 914
Bse118I RCCGGY 1 cut(s) 1117
Bse1I ACTGG 2 cut(s) 335, 610
BseDI CCNNGG 5 cut(s) 108, 198, 400, 520, 818
BseGI GGATG 1 cut(s) 541
BseLI CCNNNNNNNGG 5 cut(s) 233, 259, 341, 748, 914
BseMII CTCAG 2 cut(s) 330, 682
BseNI ACTGG 2 cut(s) 335, 610
BsePI GCGCGC 1 cut(s) 599
BseRI GAGGAG 2 cut(s) 677, 1211
Bsh1236I CGCG 1 cut(s) 601
BshFI GGCC 6 cut(s) 203, 509, 692, 719, 817, 1099
BsiHKAI GWGCWC 1 cut(s) 669
BsiHKCI CYCGRG 1 cut(s) 900
BsiSI CCGG 4 cut(s) 110, 234, 342, 1118
BslI CCNNNNNNNGG 5 cut(s) 233, 259, 341, 748, 914
BsmAI GTCTC 1 cut(s) 1254
BsnI GGCC 6 cut(s) 203, 509, 692, 719, 817, 1099
BsoBI CYCGRG 1 cut(s) 900
Bsp1286I GDGCHC 1 cut(s) 669
Bsp143I GATC 4 cut(s) 94, 676, 770, 939
BspACI CCGC 5 cut(s) 510, 558, 814, 839, 1259
BspANI GGCC 6 cut(s) 203, 509, 692, 719, 817, 1099
BspCNI CTCAG 2 cut(s) 329, 681
BspFNI CGCG 1 cut(s) 601
BspHI TCATGA 1 cut(s) 1206
BspLI GGNNCC 1 cut(s) 857
BspMI ACCTGC 2 cut(s) 1021, 1050
BspPI GGATC 1 cut(s) 778
BsrFI RCCGGY 1 cut(s) 1117
BsrI ACTGG 2 cut(s) 335, 610
BssAI RCCGGY 1 cut(s) 1117
BssECI CCNNGG 5 cut(s) 108, 198, 400, 520, 818
BssHII GCGCGC 1 cut(s) 599
BssMI GATC 4 cut(s) 94, 676, 770, 939
BssNAI GTATAC 2 cut(s) 281, 920
BssNI GRCGYC 1 cut(s) 405
BssT1I CCWWGG 3 cut(s) 198, 400, 818
Bst1107I GTATAC 2 cut(s) 281, 920
Bst4CI ACNGT 1 cut(s) 549
BstACI GRCGYC 1 cut(s) 405
BstBAI YACGTR 3 cut(s) 710, 955, 1104
BstC8I GCNNGC 4 cut(s) 468, 601, 1028, 1119
BstDEI CTNAG 3 cut(s) 316, 668, 1212
BstDSI CCRYGG 1 cut(s) 520
BstF5I GGATG 1 cut(s) 541
BstFNI CGCG 1 cut(s) 601
BstHHI GCGC 2 cut(s) 601, 603
BstKTI GATC 4 cut(s) 97, 679, 773, 942
BstMAI GTCTC 1 cut(s) 1254
BstMBI GATC 4 cut(s) 94, 676, 770, 939
BstMWI GCNNNNNNNGC 4 cut(s) 370, 515, 689, 878
BstSCI CCNGG 2 cut(s) 108, 340
BstSFI CTRYAG 1 cut(s) 965
BstSNI TACGTA 1 cut(s) 955
BstUI CGCG 1 cut(s) 601
BstV2I GAAGAC 1 cut(s) 197
BstXI CCANNNNNNTGG 1 cut(s) 851
BstZ17I GTATAC 2 cut(s) 281, 920
BsuI GTATCC 1 cut(s) 1147
BsuRI GGCC 6 cut(s) 203, 509, 692, 719, 817, 1099
BtgI CCRYGG 1 cut(s) 520
BtgZI GCGATG 1 cut(s) 1239
BtrI CACGTC 1 cut(s) 382
BtsCI GGATG 1 cut(s) 541
BtsI GCAGTG 2 cut(s) 513, 833
BtsIMutI CAGTG 5 cut(s) 168, 513, 603, 833, 1121
BveI ACCTGC 2 cut(s) 1021, 1050
Cac8I GCNNGC 4 cut(s) 468, 601, 1028, 1119
CciI TCATGA 1 cut(s) 1206
CfoI GCGC 2 cut(s) 601, 603
Cfr10I RCCGGY 1 cut(s) 1117
Cfr13I GGNCC 1 cut(s) 507
CseI GACGC 1 cut(s) 394
Csp6I GTAC 3 cut(s) 338, 952, 1072
CspCI CAANNNNNGTGG 2 cut(s) 1147, 1182
CviAII CATG 6 cut(s) 361, 800, 874, 914, 1150, 1207
CviQI GTAC 3 cut(s) 338, 952, 1072
DdeI CTNAG 3 cut(s) 316, 668, 1212
DpnI GATC 4 cut(s) 96, 678, 772, 941
DpnII GATC 4 cut(s) 94, 676, 770, 939
EaeI YGGCCR 2 cut(s) 201, 815
EciI GGCGGA 1 cut(s) 547
Ecl136II GAGCTC 1 cut(s) 667
Eco105I TACGTA 1 cut(s) 955
Eco130I CCWWGG 3 cut(s) 198, 400, 818
Eco147I AGGCCT 3 cut(s) 692, 719, 1099
Eco24I GRGCYC 1 cut(s) 669
Eco53kI GAGCTC 1 cut(s) 667
Eco57I CTGAAG 3 cut(s) 36, 209, 1030
Eco72I CACGTG 1 cut(s) 710
Eco88I CYCGRG 1 cut(s) 900
EcoICRI GAGCTC 1 cut(s) 667
EcoRI GAATTC 1 cut(s) 140
EcoT14I CCWWGG 3 cut(s) 198, 400, 818
EcoT38I GRGCYC 1 cut(s) 669
ErhI CCWWGG 3 cut(s) 198, 400, 818
FaeI CATG 6 cut(s) 364, 803, 877, 917, 1153, 1210
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FatI CATG 6 cut(s) 360, 799, 873, 913, 1149, 1206
FbaI TGATCA 1 cut(s) 94
FblI GTMKAC 2 cut(s) 280, 919
Fnu4HI GCNGC 3 cut(s) 510, 815, 839
FokI GGATG 1 cut(s) 528
FriOI GRGCYC 1 cut(s) 669
Fsp4HI GCNGC 3 cut(s) 510, 815, 839
FspBI CTAG 2 cut(s) 230, 1139
GlaI GCGC 2 cut(s) 600, 602
GluI GCNGC 3 cut(s) 510, 815, 839
HaeIII GGCC 6 cut(s) 203, 509, 692, 719, 817, 1099
HapII CCGG 4 cut(s) 110, 234, 342, 1118
HgaI GACGC 1 cut(s) 394
HhaI GCGC 2 cut(s) 601, 603
Hin1I GRCGYC 1 cut(s) 405
Hin1II CATG 6 cut(s) 364, 803, 877, 917, 1153, 1210
Hin6I GCGC 2 cut(s) 599, 601
HinP1I GCGC 2 cut(s) 599, 601
HinfI GANTC 1 cut(s) 240
HpaII CCGG 4 cut(s) 110, 234, 342, 1118
HphI GGTGA 2 cut(s) 536, 986
Hpy166II GTNNAC 6 cut(s) 281, 304, 527, 808, 920, 1144
Hpy188I TCNGA 3 cut(s) 319, 671, 885
Hpy188III TCNNGA 7 cut(s) 92, 326, 736, 900, 997, 1132, 1207
Hpy8I GTNNAC 6 cut(s) 281, 304, 527, 808, 920, 1144
Hpy99I CGWCG 1 cut(s) 383
HpyAV CCTTC 2 cut(s) 54, 714
HpyCH4III ACNGT 1 cut(s) 549
HpyCH4IV ACGT 6 cut(s) 381, 491, 709, 954, 1103, 1127
HpyCH4V TGCA 3 cut(s) 267, 309, 1059
HpyF10VI GCNNNNNNNGC 4 cut(s) 370, 515, 689, 878
HpyF3I CTNAG 3 cut(s) 316, 668, 1212
HpySE526I ACGT 6 cut(s) 381, 491, 709, 954, 1103, 1127
Hsp92I GRCGYC 1 cut(s) 405
Hsp92II CATG 6 cut(s) 364, 803, 877, 917, 1153, 1210
HspAI GCGC 2 cut(s) 599, 601
KroI GCCGGC 1 cut(s) 1117
KroNI GCCGGC 1 cut(s) 1119
Ksp22I TGATCA 1 cut(s) 94
Kzo9I GATC 4 cut(s) 94, 676, 770, 939
LmnI GCTCC 3 cut(s) 664, 886, 1231
LweI GCATC 1 cut(s) 586
MaeI CTAG 2 cut(s) 230, 1139
MaeII ACGT 6 cut(s) 381, 491, 709, 954, 1103, 1127
MaeIII GTNAC 2 cut(s) 625, 1033
MalI GATC 4 cut(s) 96, 678, 772, 941
MboI GATC 4 cut(s) 94, 676, 770, 939
MboII GAAGA 4 cut(s) 39, 59, 202, 717
MhlI GDGCHC 1 cut(s) 669
MlsI TGGCCA 1 cut(s) 203
MluCI AATT 5 cut(s) 140, 254, 501, 580, 1038
MluNI TGGCCA 1 cut(s) 203
MlyI GAGTC 1 cut(s) 234
MmeI TCCRAC 2 cut(s) 77, 204
Mox20I TGGCCA 1 cut(s) 203
MroNI GCCGGC 1 cut(s) 1117
MscI TGGCCA 1 cut(s) 203
MseI TTAA 3 cut(s) 212, 584, 695
Msp20I TGGCCA 1 cut(s) 203
MspA1I CMGCKG 1 cut(s) 776
MspI CCGG 4 cut(s) 110, 234, 342, 1118
MspR9I CCNGG 2 cut(s) 110, 342
MvnI CGCG 1 cut(s) 601
MwoI GCNNNNNNNGC 4 cut(s) 370, 515, 689, 878
NaeI GCCGGC 1 cut(s) 1119
NciI CCSGG 2 cut(s) 110, 342
NdeII GATC 4 cut(s) 94, 676, 770, 939
NgoMIV GCCGGC 1 cut(s) 1117
NlaIII CATG 6 cut(s) 364, 803, 877, 917, 1153, 1210
NlaIV GGNNCC 1 cut(s) 857
NmuCI GTSAC 1 cut(s) 625
PagI TCATGA 1 cut(s) 1206
PauI GCGCGC 1 cut(s) 599
PceI AGGCCT 3 cut(s) 692, 719, 1099
PcsI WCGNNNNNNNCGW 1 cut(s) 706
PdiI GCCGGC 1 cut(s) 1119
PflMI CCANNNNNTGG 2 cut(s) 259, 914
PkrI GCNGC 3 cut(s) 511, 816, 840
PleI GAGTC 1 cut(s) 234
PmaCI CACGTG 1 cut(s) 710
PmlI CACGTG 1 cut(s) 710
PpsI GAGTC 1 cut(s) 234
Ppu21I YACGTR 3 cut(s) 710, 955, 1104
Psp124BI GAGCTC 1 cut(s) 669
PspCI CACGTG 1 cut(s) 710
PspN4I GGNNCC 1 cut(s) 857
PspPI GGNCC 1 cut(s) 507
PteI GCGCGC 1 cut(s) 599
PvuII CAGCTG 1 cut(s) 776
RsaI GTAC 3 cut(s) 339, 953, 1073
RsaNI GTAC 3 cut(s) 338, 952, 1072
SacI GAGCTC 1 cut(s) 669
SaqAI TTAA 3 cut(s) 212, 584, 695
SatI GCNGC 3 cut(s) 510, 815, 839
Sau3AI GATC 4 cut(s) 94, 676, 770, 939
Sau96I GGNCC 1 cut(s) 507
SchI GAGTC 1 cut(s) 234
ScrFI CCNGG 2 cut(s) 110, 342
SduI GDGCHC 1 cut(s) 669
SfaNI GCATC 1 cut(s) 586
SfcI CTRYAG 1 cut(s) 965
SmlI CTYRAG 1 cut(s) 736
SmoI CTYRAG 1 cut(s) 736
SnaBI TACGTA 1 cut(s) 955
Sse9I AATT 5 cut(s) 140, 254, 501, 580, 1038
SseBI AGGCCT 3 cut(s) 692, 719, 1099
SsiI CCGC 5 cut(s) 510, 558, 814, 839, 1259
SspMI CTAG 2 cut(s) 230, 1139
SstI GAGCTC 1 cut(s) 669
StuI AGGCCT 3 cut(s) 692, 719, 1099
StyD4I CCNGG 2 cut(s) 108, 340
StyI CCWWGG 3 cut(s) 198, 400, 818
TaaI ACNGT 1 cut(s) 549
TaiI ACGT 6 cut(s) 384, 494, 712, 957, 1106, 1130
TaqI TCGA 3 cut(s) 327, 825, 942
TasI AATT 5 cut(s) 140, 254, 501, 580, 1038
TauI GCSGC 3 cut(s) 512, 817, 841
Tru1I TTAA 3 cut(s) 212, 584, 695
Tru9I TTAA 3 cut(s) 212, 584, 695
TscAI CASTG 5 cut(s) 175, 520, 610, 840, 1128
TseFI GTSAC 1 cut(s) 625
Tsp45I GTSAC 1 cut(s) 625
TspDTI ATGAA 6 cut(s) 17, 153, 541, 721, 788, 1138
TspRI CASTG 5 cut(s) 175, 520, 610, 840, 1128
Van91I CCANNNNNTGG 2 cut(s) 259, 914
XapI RAATTY 3 cut(s) 140, 501, 580
XmiI GTMKAC 2 cut(s) 280, 919
XspI CTAG 2 cut(s) 230, 1139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.