MD16G1108800.v1.1

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000622
Physical Location & Seq
Forward (+)
7620907 .. 7621395
489 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1108800.v1.1.491

Sequence Viewer

Length: 489 bp
ATGAAAGTCGAAGTTGAAGTAATCTCCAAGGAGTTTATCAGACCGTCTTCTCCAACACCTGATCACCATTGCACGTACCAACTCTCATTTCTCAACCAGATATCTCCTCCGGTCTATAATCCTATTGTCATATTCTATTCCTCATCAGATCCCGAAACAAAATTCGACATTGTCGAAATATCCAATCAGCTTAAGATATCCCTATCCGATGTCTTGACTCAATTCTATCCGCTAGCTGGACGTGCCCAAGGCAACTTGTTCATAGACAGCAATGACCAGGGCATACCCTACTTAGAAACTAGAGTTAAGTGCCAACTTTCCGACGTTATTGAAAACCCAATTCCTATTGTACTCAACAAATTCATTCCATTCCAAGTAGATGAAGTCACTGGTATTTCCCTCGGTGTTCAACTCAACATCTTTGAGCCTGGTGGCATAGCTCTTGGTCTCTGCATTTCCCATAAGATTTCGGATGCCTTATCGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

163

Amino Acids

18.01

Weight (kDa)

4.68

Isoelectric Point (pI)

33.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 4 - 162 5.6e-32 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24710 FvH4_4g24720 FvH4_4g24770 FvH4_4g24771 FvH4_4g24772 FvH4_5g39380
malus_domestica MD10G1199500.v1.1 MD13G1109100.v1.1 MD13G1109200.v1.1 MD13G1114400.v1.1 MD13G1114800.v1.1 MD16G1108800.v1.1
prunus_persica Prupe.1G235900_v2.0.a1 Prupe.1G236600_v2.0.a1 Prupe.1G236700_v2.0.a1 Prupe.1G236800_v2.0.a1 Prupe.4G142000_v2.0.a1
pyrus_communis pycom10g17180 pycom13g09460 pycom13g09930 pycom13g09940
rosa_chinensis RchiOBHm_Chr3g0490281 RchiOBHm_Chr3g0490291 RchiOBHm_Chr3g0490301 RchiOBHm_Chr4g0417441 RchiOBHm_Chr4g0417461 RchiOBHm_Chr4g0431811 RchiOBHm_Chr4g0431821 RchiOBHm_Chr4g0431851
rosa_laevigata RLG00000006882 RLG00000006886 RLG00000006887 RLG00000007905 RLG00000007911 RLG00000007913 RLG00000029538
rosa_multiflora Rmu_sc0001459.1_g000008 Rmu_sc0001459.1_g000011 Rmu_sc0002489.1_g000020 Rmu_sc0002489.1_g000024 Rmu_sc0002489.1_g000027 Rmu_sc0002489.1_g000028 Rmu_sc0002489.1_g000029 Rmu_sc0004142.1_g000001 Rmu_sc0004142.1_g000024 Rmu_sc0011237.1_g000018 Rmu_sc0031439.1_g000001 Rmu_sc0031677.1_g000001 Rmu_ssc0000361.1_g000005
rosa_roxburghii Rroxscaffold_3G00262760 Rroxscaffold_4G00317310 Rroxscaffold_5G00361150 Rroxscaffold_5G00373190 Rroxscaffold_5G00373210 Rroxscaffold_5G00373220 Rroxscaffold_5G00373240
rosa_rugosa Rorug01G0111100 Rorug04G0252400 Rorug04G0252400 Rorug04G0252400 Rorug04G0252600
rosa_samantha Rh1BG102300 Rh3AG292200 Rh3AG292300 Rh4AG309100 Rh4AG309300 Rh4AG309500 Rh6BG200900
rosa_wichuraiana Rw0G002990 Rw0G003000 Rw0G019170 Rw3G025890 Rw3G025900 Rw4G017830 Rw4G026810 Rw4G026870 Rw4G026880 Rw4G026920 Rw6G017190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 230
AclWI GGATC 1 cut(s) 143
AcsI RAATTY 2 cut(s) 161, 359
AfaI GTAC 2 cut(s) 77, 351
AfiI CCNNNNNNNGG 1 cut(s) 236
AflII CTTAAG 1 cut(s) 191
AgsI TTSAA 3 cut(s) 17, 332, 410
AjiI CACGTC 1 cut(s) 242
AjnI CCWGG 2 cut(s) 276, 427
AjuI GAANNNNNNNTTGG 2 cut(s) 72, 104
AluBI AGCT 3 cut(s) 190, 236, 440
AluI AGCT 3 cut(s) 190, 236, 440
Alw26I GTCTC 1 cut(s) 452
AlwI GGATC 1 cut(s) 143
ApoI RAATTY 2 cut(s) 161, 359
AsuHPI GGTGA 1 cut(s) 56
AsuNHI GCTAGC 1 cut(s) 232
BaeGI GKGCMC 1 cut(s) 247
BbsI GAAGAC 1 cut(s) 39
BciT130I CCWGG 2 cut(s) 278, 429
BclI TGATCA 1 cut(s) 61
BcoDI GTCTC 1 cut(s) 452
BfaI CTAG 2 cut(s) 233, 300
BfrI CTTAAG 1 cut(s) 191
Bme1390I CCNGG 2 cut(s) 278, 429
BmgBI CACGTC 1 cut(s) 242
BmrFI CCNGG 2 cut(s) 278, 429
BmsI GCATC 1 cut(s) 463
BmtI GCTAGC 1 cut(s) 236
BpiI GAAGAC 1 cut(s) 39
BsaAI YACGTR 1 cut(s) 75
BsaI GGTCTC 1 cut(s) 452
BsaJI CCNNGG 4 cut(s) 27, 247, 277, 400
BsaWI WCCGGW 1 cut(s) 109
Bsc4I CCNNNNNNNGG 1 cut(s) 236
Bse1I ACTGG 1 cut(s) 394
Bse3DI GCAATG 2 cut(s) 67, 277
BseBI CCWGG 2 cut(s) 278, 429
BseDI CCNNGG 4 cut(s) 27, 247, 277, 400
BseGI GGATG 1 cut(s) 478
BseLI CCNNNNNNNGG 1 cut(s) 236
BseMI GCAATG 2 cut(s) 67, 277
BseNI ACTGG 1 cut(s) 394
BseRI GAGGAG 1 cut(s) 96
BseSI GKGCMC 1 cut(s) 247
BsiSI CCGG 1 cut(s) 110
BslI CCNNNNNNNGG 1 cut(s) 236
BsmAI GTCTC 1 cut(s) 452
Bso31I GGTCTC 1 cut(s) 452
Bsp1286I GDGCHC 1 cut(s) 247
Bsp143I GATC 2 cut(s) 61, 148
BspACI CCGC 1 cut(s) 230
BspOI GCTAGC 1 cut(s) 236
BspPI GGATC 1 cut(s) 143
BspTI CTTAAG 1 cut(s) 191
BspTNI GGTCTC 1 cut(s) 452
BsrDI GCAATG 2 cut(s) 67, 277
BsrI ACTGG 1 cut(s) 394
BssECI CCNNGG 4 cut(s) 27, 247, 277, 400
BssMI GATC 2 cut(s) 61, 148
BssT1I CCWWGG 2 cut(s) 27, 247
Bst2UI CCWGG 2 cut(s) 278, 429
Bst4CI ACNGT 1 cut(s) 45
BstAFI CTTAAG 1 cut(s) 191
BstBAI YACGTR 1 cut(s) 75
BstC8I GCNNGC 1 cut(s) 234
BstDEI CTNAG 1 cut(s) 292
BstF5I GGATG 1 cut(s) 478
BstKTI GATC 2 cut(s) 64, 151
BstMAI GTCTC 1 cut(s) 452
BstMBI GATC 2 cut(s) 61, 148
BstMWI GCNNNNNNNGC 1 cut(s) 242
BstNI CCWGG 2 cut(s) 278, 429
BstSCI CCNGG 2 cut(s) 276, 427
BstSLI GKGCMC 1 cut(s) 247
BstV2I GAAGAC 1 cut(s) 39
BstX2I RGATCY 1 cut(s) 148
BstYI RGATCY 1 cut(s) 148
BtrI CACGTC 1 cut(s) 242
BtsCI GGATG 1 cut(s) 478
BtsIMutI CAGTG 1 cut(s) 387
Cac8I GCNNGC 1 cut(s) 234
Csp6I GTAC 2 cut(s) 76, 350
CviJI RGCY 4 cut(s) 190, 236, 427, 440
CviKI_1 RGCY 4 cut(s) 190, 236, 427, 440
CviQI GTAC 2 cut(s) 76, 350
DdeI CTNAG 1 cut(s) 292
DpnI GATC 2 cut(s) 63, 150
DpnII GATC 2 cut(s) 61, 148
Eco130I CCWWGG 2 cut(s) 27, 247
Eco31I GGTCTC 1 cut(s) 452
Eco32I GATATC 2 cut(s) 102, 198
EcoRII CCWGG 2 cut(s) 276, 427
EcoRV GATATC 2 cut(s) 102, 198
EcoT14I CCWWGG 2 cut(s) 27, 247
ErhI CCWWGG 2 cut(s) 27, 247
FaiI YATR 6 cut(s) 117, 131, 263, 284, 437, 462
FbaI TGATCA 1 cut(s) 61
FokI GGATG 1 cut(s) 485
FspBI CTAG 2 cut(s) 233, 300
HapII CCGG 1 cut(s) 110
HinfI GANTC 1 cut(s) 217
HpaII CCGG 1 cut(s) 110
HphI GGTGA 1 cut(s) 56
Hpy188I TCNGA 5 cut(s) 41, 148, 208, 322, 472
Hpy188III TCNNGA 2 cut(s) 152, 214
Hpy99I CGWCG 1 cut(s) 326
HpyCH4III ACNGT 1 cut(s) 45
HpyCH4IV ACGT 3 cut(s) 74, 241, 324
HpyCH4V TGCA 2 cut(s) 72, 453
HpyF10VI GCNNNNNNNGC 1 cut(s) 242
HpyF3I CTNAG 1 cut(s) 292
HpySE526I ACGT 3 cut(s) 74, 241, 324
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 2 cut(s) 61, 148
LpnPI CCDG 9 cut(s) 72, 110, 123, 222, 263, 290, 375, 414, 441
LweI GCATC 1 cut(s) 463
MaeI CTAG 2 cut(s) 233, 300
MaeII ACGT 3 cut(s) 74, 241, 324
MaeIII GTNAC 1 cut(s) 385
MalI GATC 2 cut(s) 63, 150
MboI GATC 2 cut(s) 61, 148
MboII GAAGA 1 cut(s) 39
MflI RGATCY 1 cut(s) 148
MhlI GDGCHC 1 cut(s) 247
MluCI AATT 4 cut(s) 161, 221, 339, 359
MlyI GAGTC 1 cut(s) 211
MmeI TCCRAC 2 cut(s) 77, 345
MnlI CCTC 3 cut(s) 117, 151, 410
MseI TTAA 3 cut(s) 192, 306, 487
MspCI CTTAAG 1 cut(s) 191
MspI CCGG 1 cut(s) 110
MspR9I CCNGG 2 cut(s) 278, 429
MvaI CCWGG 2 cut(s) 278, 429
MwoI GCNNNNNNNGC 1 cut(s) 242
NdeII GATC 2 cut(s) 61, 148
NheI GCTAGC 1 cut(s) 232
NmuCI GTSAC 1 cut(s) 385
PcsI WCGNNNNNNNCGW 1 cut(s) 171
PflFI GACNNNGTC 1 cut(s) 170
PleI GAGTC 1 cut(s) 211
PpsI GAGTC 1 cut(s) 211
Ppu21I YACGTR 1 cut(s) 75
Psp6I CCWGG 2 cut(s) 276, 427
PspGI CCWGG 2 cut(s) 276, 427
PsuI RGATCY 1 cut(s) 148
PsyI GACNNNGTC 1 cut(s) 170
RsaI GTAC 2 cut(s) 77, 351
RsaNI GTAC 2 cut(s) 76, 350
SaqAI TTAA 3 cut(s) 192, 306, 487
Sau3AI GATC 2 cut(s) 61, 148
SchI GAGTC 1 cut(s) 211
ScrFI CCNGG 2 cut(s) 278, 429
SduI GDGCHC 1 cut(s) 247
SetI ASST 7 cut(s) 61, 77, 192, 238, 244, 327, 442
SfaNI GCATC 1 cut(s) 463
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
Sse9I AATT 4 cut(s) 161, 221, 339, 359
SsiI CCGC 1 cut(s) 230
SspMI CTAG 2 cut(s) 233, 300
StyD4I CCNGG 2 cut(s) 276, 427
StyI CCWWGG 2 cut(s) 27, 247
TaaI ACNGT 1 cut(s) 45
TaiI ACGT 3 cut(s) 77, 244, 327
TaqI TCGA 3 cut(s) 9, 165, 174
TasI AATT 4 cut(s) 161, 221, 339, 359
TatI WGTACW 1 cut(s) 349
Tru1I TTAA 3 cut(s) 192, 306, 487
Tru9I TTAA 3 cut(s) 192, 306, 487
TscAI CASTG 1 cut(s) 394
TseFI GTSAC 1 cut(s) 385
Tsp45I GTSAC 1 cut(s) 385
TspDTI ATGAA 4 cut(s) 17, 250, 352, 396
TspRI CASTG 1 cut(s) 394
Tth111I GACNNNGTC 1 cut(s) 170
Vha464I CTTAAG 1 cut(s) 191
XapI RAATTY 2 cut(s) 161, 359
XspI CTAG 2 cut(s) 233, 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.