MD13G1114400.v1.1

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000622
Physical Location & Seq
Reverse (-)
8286588 .. 8287919
1332 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1114400.v1.1.491

Sequence Viewer

Length: 1332 bp
ATGAAGGTTCAAGTTGTAGTAATCTCCAAGGAGATTATCAGACCATCTTCTCCAACTCCTGGCCACCTTCGCCATTACCAATTCTCCTTTCTTGATCAAATGGCTCCCCAAGTCTACAACCCTTTGCTCCTCTTCTATGAATACAATGCCAAGATGCATCCCAACATCACTGAAATATCCAACCACCTTAAGAACTCCTTATCCGAGGCCCTATCGCTATTCTACCCGCTAGCCGGTCGAATAAAAGACAACTGCTTCATACATTGCAATGACGAGGGCATCCCCTACATCGAAGCTCGAGTGCTGAATTGCACTCTCTCTGATGTTCTCAGCAACCCCAACCCTGATGAACTTAACAAGTTCATGCCATTTGAACTCGATGATATTACCAATCCATTACCCTTTGGGGTCCAGCTCAACATATTTCAGTGTGGAGGGTTTGCTATTGGCCAATGCGTTTCTCACAAGATTGCTGATGGGTTGTCCTATTTCACGTTCAGCAAAATTTGGGCAGCCATTGCCCGTGGGGAACAAGCCAACATAGACCCTCCACAATTTGTGTCAGCCACACTCTTCCCACCAAAGGAGTTTAATACTGGATTCGATGGAGGCGTTGGAATCACAAAAGATAGAGTGACAAAGAGGTTCGTGTTCAATGCCTCTAAAATAAAGACCCTCCAAGCAAAATATGAAAAAGATATGAAAAGCCTCCAACTAAAACACCCCACTCGTGTTGAGACCTTATCCGCTTTCATATGGAGTCGATATGTGGCGGCTACAAAGGATTGTACCGTGAATAAGTTGTACATGGTGATCCACGCAGTGAACCTACGTCCGCGGTTTAGTCCACCATTGCCTCAACATTCTTTTGGGAATCTTTTTCGTGTTGCCATGACATCACCGCTGCGAATCAGTAATGGCGGGGAGGAAGAATGTGATGGGGTGGTGATTGGGGAGGTGCGAGAAGCGATAAGCAAGATTGACAATGAGTACGTGAAGCAATTACAAGGGCAGGGTGATGAGCACTTGGGTTTGATGAAGAAAGCTGCTGATAGTTTCAAGAGAGGAGACATGGTTGCGGTACTTAGCTTCAGTAGCTATTGTAGGTTTCCTCTCTATGAGAATGACTTTGGTTGGGGTAAACCTGCGTGGGTGGGATCACCGGCACTGACCTATAAGAACCTAGTGCTTTTCATGGACACCAAGGAGGGTGATGGAATAGAGGCATATGTTAGCTTGGAGGAGAGAGTCATGGTCAAATTCGAATGTGATAGCGAGTTGCTGTCTTATGTTTCTCCAGACGGTCGGGTCCTGCTGAGCTGCGGAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

444

Amino Acids

49.76

Weight (kDa)

6.38

Isoelectric Point (pI)

45.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 4 - 427 2.5e-84 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24710 FvH4_4g24720 FvH4_4g24770 FvH4_4g24771 FvH4_4g24772 FvH4_5g39380
malus_domestica MD10G1199500.v1.1 MD13G1109100.v1.1 MD13G1109200.v1.1 MD13G1114400.v1.1 MD13G1114800.v1.1 MD16G1108800.v1.1
prunus_persica Prupe.1G235900_v2.0.a1 Prupe.1G236600_v2.0.a1 Prupe.1G236700_v2.0.a1 Prupe.1G236800_v2.0.a1 Prupe.4G142000_v2.0.a1
pyrus_communis pycom10g17180 pycom13g09460 pycom13g09930 pycom13g09940
rosa_chinensis RchiOBHm_Chr3g0490281 RchiOBHm_Chr3g0490291 RchiOBHm_Chr3g0490301 RchiOBHm_Chr4g0417441 RchiOBHm_Chr4g0417461 RchiOBHm_Chr4g0431811 RchiOBHm_Chr4g0431821 RchiOBHm_Chr4g0431851
rosa_laevigata RLG00000006882 RLG00000006886 RLG00000006887 RLG00000007905 RLG00000007911 RLG00000007913 RLG00000029538
rosa_multiflora Rmu_sc0001459.1_g000008 Rmu_sc0001459.1_g000011 Rmu_sc0002489.1_g000020 Rmu_sc0002489.1_g000024 Rmu_sc0002489.1_g000027 Rmu_sc0002489.1_g000028 Rmu_sc0002489.1_g000029 Rmu_sc0004142.1_g000001 Rmu_sc0004142.1_g000024 Rmu_sc0011237.1_g000018 Rmu_sc0031439.1_g000001 Rmu_sc0031677.1_g000001 Rmu_ssc0000361.1_g000005
rosa_roxburghii Rroxscaffold_3G00262760 Rroxscaffold_4G00317310 Rroxscaffold_5G00361150 Rroxscaffold_5G00373190 Rroxscaffold_5G00373210 Rroxscaffold_5G00373220 Rroxscaffold_5G00373240
rosa_rugosa Rorug01G0111100 Rorug04G0252400 Rorug04G0252400 Rorug04G0252400 Rorug04G0252600
rosa_samantha Rh1BG102300 Rh3AG292200 Rh3AG292300 Rh4AG309100 Rh4AG309300 Rh4AG309500 Rh6BG200900
rosa_wichuraiana Rw0G002990 Rw0G003000 Rw0G019170 Rw3G025890 Rw3G025900 Rw4G017830 Rw4G026810 Rw4G026870 Rw4G026880 Rw4G026920 Rw6G017190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1307
Acc36I ACCTGC 1 cut(s) 1153
AccB7I CCANNNNNTGG 1 cut(s) 59
AccI GTMKAC 1 cut(s) 114
AccII CGCG 1 cut(s) 838
AciI CCGC 9 cut(s) 227, 747, 773, 836, 838, 902, 921, 1079, 1323
AclWI GGATC 2 cut(s) 808, 1165
AcoI YGGCCR 2 cut(s) 61, 448
AcsI RAATTY 2 cut(s) 504, 1259
AcuI CTGAAG 1 cut(s) 1075
AdeI CACNNNGTG 1 cut(s) 823
AfaI GTAC 4 cut(s) 790, 806, 992, 1083
AfiI CCNNNNNNNGG 3 cut(s) 59, 233, 583
AflII CTTAAG 1 cut(s) 188
AgsI TTSAA 4 cut(s) 11, 374, 655, 1060
AjnI CCWGG 1 cut(s) 58
AjuI GAANNNNNNNTTGG 2 cut(s) 72, 104
AloI GAACNNNNNNTCC 2 cut(s) 185, 217
AluBI AGCT 7 cut(s) 296, 415, 1046, 1089, 1098, 1236, 1320
AluI AGCT 7 cut(s) 296, 415, 1046, 1089, 1098, 1236, 1320
Alw21I GWGCWC 1 cut(s) 1026
Alw26I GTCTC 2 cut(s) 731, 1062
AlwI GGATC 2 cut(s) 808, 1165
Ama87I CYCGRG 1 cut(s) 297
AoxI GGCC 3 cut(s) 61, 207, 448
ApeKI GCWGC 4 cut(s) 512, 904, 1046, 1320
ApoI RAATTY 2 cut(s) 504, 1259
AspS9I GGNCC 3 cut(s) 208, 409, 1309
AsuHPI GGTGA 6 cut(s) 823, 891, 958, 1028, 1152, 1223
AsuII TTCGAA 1 cut(s) 1263
AsuNHI GCTAGC 1 cut(s) 229
AvaI CYCGRG 1 cut(s) 297
AvaII GGWCC 2 cut(s) 409, 1309
BalI TGGCCA 2 cut(s) 63, 450
BauI CACGAG 1 cut(s) 729
Bbv12I GWGCWC 1 cut(s) 1026
BbvI GCAGC 4 cut(s) 524, 891, 1033, 1307
BccI CCATC 5 cut(s) 52, 470, 599, 932, 1208
BciT130I CCWGG 1 cut(s) 60
BclI TGATCA 1 cut(s) 94
BcoDI GTCTC 2 cut(s) 731, 1062
BfaI CTAG 2 cut(s) 230, 1184
BfrI CTTAAG 1 cut(s) 188
BfuAI ACCTGC 1 cut(s) 1153
BisI GCNGC 5 cut(s) 513, 774, 905, 1047, 1321
BlpI GCTNAGC 1 cut(s) 1316
BlsI GCNGC 5 cut(s) 514, 775, 906, 1048, 1322
Bme1390I CCNGG 1 cut(s) 60
Bme18I GGWCC 2 cut(s) 409, 1309
BmeT110I CYCGRG 1 cut(s) 297
BmgT120I GGNCC 3 cut(s) 208, 409, 1309
BmiI GGNNCC 3 cut(s) 105, 410, 1310
BmrFI CCNGG 1 cut(s) 60
BmsI GCATC 3 cut(s) 144, 166, 288
BmtI GCTAGC 1 cut(s) 233
BpmI CTGGAG 1 cut(s) 1281
Bpu1102I GCTNAGC 1 cut(s) 1316
Bpu14I TTCGAA 1 cut(s) 1263
BsaAI YACGTR 1 cut(s) 994
BsaI GGTCTC 1 cut(s) 731
BsaJI CCNNGG 5 cut(s) 27, 204, 523, 836, 1203
BsaXI ACNNNNNCTCC 4 cut(s) 68, 98, 1059, 1089
Bsc4I CCNNNNNNNGG 3 cut(s) 59, 233, 583
Bse118I RCCGGY 2 cut(s) 233, 1162
Bse1I ACTGG 1 cut(s) 601
Bse3DI GCAATG 4 cut(s) 262, 274, 516, 851
BseBI CCWGG 1 cut(s) 60
BseDI CCNNGG 5 cut(s) 27, 204, 523, 836, 1203
BseGI GGATG 2 cut(s) 157, 279
BseLI CCNNNNNNNGG 3 cut(s) 59, 233, 583
BseMI GCAATG 4 cut(s) 262, 274, 516, 851
BseMII CTCAG 2 cut(s) 343, 1307
BseNI ACTGG 1 cut(s) 601
BseRI GAGGAG 3 cut(s) 119, 1080, 1256
BseXI GCAGC 4 cut(s) 524, 891, 1033, 1307
Bsh1236I CGCG 1 cut(s) 838
Bsh1285I CGRYCG 2 cut(s) 238, 1306
BshFI GGCC 3 cut(s) 63, 209, 450
BsiEI CGRYCG 2 cut(s) 238, 1306
BsiHKAI GWGCWC 1 cut(s) 1026
BsiHKCI CYCGRG 1 cut(s) 297
BsiSI CCGG 2 cut(s) 234, 1163
BslI CCNNNNNNNGG 3 cut(s) 59, 233, 583
BsmAI GTCTC 2 cut(s) 731, 1062
BsnI GGCC 3 cut(s) 63, 209, 450
Bso31I GGTCTC 1 cut(s) 731
BsoBI CYCGRG 1 cut(s) 297
Bsp119I TTCGAA 1 cut(s) 1263
Bsp1286I GDGCHC 1 cut(s) 1026
Bsp1407I TGTACA 1 cut(s) 804
Bsp143I GATC 3 cut(s) 94, 813, 1157
Bsp1720I GCTNAGC 1 cut(s) 1316
BspACI CCGC 9 cut(s) 227, 747, 773, 836, 838, 902, 921, 1079, 1323
BspANI GGCC 3 cut(s) 63, 209, 450
BspCNI CTCAG 2 cut(s) 342, 1308
BspFNI CGCG 1 cut(s) 838
BspLI GGNNCC 3 cut(s) 105, 410, 1310
BspMI ACCTGC 1 cut(s) 1153
BspOI GCTAGC 1 cut(s) 233
BspPI GGATC 2 cut(s) 808, 1165
BspT104I TTCGAA 1 cut(s) 1263
BspTI CTTAAG 1 cut(s) 188
BspTNI GGTCTC 1 cut(s) 731
BsrDI GCAATG 4 cut(s) 262, 274, 516, 851
BsrFI RCCGGY 2 cut(s) 233, 1162
BsrGI TGTACA 1 cut(s) 804
BsrI ACTGG 1 cut(s) 601
BssAI RCCGGY 2 cut(s) 233, 1162
BssECI CCNNGG 5 cut(s) 27, 204, 523, 836, 1203
BssMI GATC 3 cut(s) 94, 813, 1157
BssSI CACGAG 1 cut(s) 729
BssT1I CCWWGG 2 cut(s) 27, 1203
Bst2BI CACGAG 1 cut(s) 729
Bst2UI CCWGG 1 cut(s) 60
Bst4CI ACNGT 2 cut(s) 793, 1304
Bst6I CTCTTC 2 cut(s) 137, 578
BstAFI CTTAAG 1 cut(s) 188
BstAPI GCANNNNNTGC 1 cut(s) 518
BstAUI TGTACA 1 cut(s) 804
BstBAI YACGTR 1 cut(s) 994
BstBI TTCGAA 1 cut(s) 1263
BstC8I GCNNGC 1 cut(s) 231
BstDEI CTNAG 3 cut(s) 329, 1085, 1316
BstDSI CCRYGG 2 cut(s) 523, 836
BstF5I GGATG 2 cut(s) 157, 279
BstFNI CGCG 1 cut(s) 838
BstKTI GATC 3 cut(s) 97, 816, 1160
BstMAI GTCTC 2 cut(s) 731, 1062
BstMBI GATC 3 cut(s) 94, 813, 1157
BstMCI CGRYCG 2 cut(s) 238, 1306
BstMWI GCNNNNNNNGC 3 cut(s) 69, 518, 1095
BstNI CCWGG 1 cut(s) 60
BstSCI CCNGG 1 cut(s) 58
BstUI CGCG 1 cut(s) 838
BstV1I GCAGC 4 cut(s) 524, 891, 1033, 1307
BsuRI GGCC 3 cut(s) 63, 209, 450
BtgI CCRYGG 2 cut(s) 523, 836
BtsCI GGATG 2 cut(s) 157, 279
BtsI GCAGTG 1 cut(s) 828
BtsIMutI CAGTG 4 cut(s) 168, 434, 828, 1166
BveI ACCTGC 1 cut(s) 1153
Cac8I GCNNGC 1 cut(s) 231
Cfr10I RCCGGY 2 cut(s) 233, 1162
Cfr13I GGNCC 3 cut(s) 208, 409, 1309
Cfr42I CCGCGG 1 cut(s) 839
Csp6I GTAC 4 cut(s) 789, 805, 991, 1082
CspCI CAANNNNNGTGG 2 cut(s) 715, 750
CviAII CATG 6 cut(s) 364, 808, 892, 1072, 1195, 1252
CviQI GTAC 4 cut(s) 789, 805, 991, 1082
DdeI CTNAG 3 cut(s) 329, 1085, 1316
DpnI GATC 3 cut(s) 96, 815, 1159
DpnII GATC 3 cut(s) 94, 813, 1157
DraIII CACNNNGTG 1 cut(s) 823
DrdI GACNNNNNNGTC 1 cut(s) 1307
DseDI GACNNNNNNGTC 1 cut(s) 1307
EaeI YGGCCR 2 cut(s) 61, 448
Eam1104I CTCTTC 2 cut(s) 137, 578
EarI CTCTTC 2 cut(s) 137, 578
Eco130I CCWWGG 2 cut(s) 27, 1203
Eco31I GGTCTC 1 cut(s) 731
Eco47I GGWCC 2 cut(s) 409, 1309
Eco57I CTGAAG 1 cut(s) 1075
Eco88I CYCGRG 1 cut(s) 297
EcoO109I RGGNCCY 2 cut(s) 208, 1309
EcoRII CCWGG 1 cut(s) 58
EcoT14I CCWWGG 2 cut(s) 27, 1203
EcoT22I ATGCAT 1 cut(s) 159
ErhI CCWWGG 2 cut(s) 27, 1203
FaeI CATG 6 cut(s) 367, 811, 895, 1075, 1198, 1255
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FatI CATG 6 cut(s) 363, 807, 891, 1071, 1194, 1251
FauI CCCGC 2 cut(s) 234, 914
FauNDI CATATG 2 cut(s) 755, 1228
FbaI TGATCA 1 cut(s) 94
FblI GTMKAC 1 cut(s) 114
Fnu4HI GCNGC 5 cut(s) 513, 774, 905, 1047, 1321
FokI GGATG 2 cut(s) 144, 266
Fsp4HI GCNGC 5 cut(s) 513, 774, 905, 1047, 1321
FspBI CTAG 2 cut(s) 230, 1184
GluI GCNGC 5 cut(s) 513, 774, 905, 1047, 1321
GsuI CTGGAG 1 cut(s) 1281
HaeIII GGCC 3 cut(s) 63, 209, 450
HapII CCGG 2 cut(s) 234, 1163
Hin1II CATG 6 cut(s) 367, 811, 895, 1075, 1198, 1255
HinfI GANTC 6 cut(s) 600, 618, 760, 874, 909, 1248
HpaII CCGG 2 cut(s) 234, 1163
HphI GGTGA 6 cut(s) 823, 891, 958, 1028, 1152, 1223
Hpy166II GTNNAC 4 cut(s) 115, 826, 848, 1142
Hpy188I TCNGA 3 cut(s) 41, 205, 322
Hpy188III TCNNGA 3 cut(s) 92, 1060, 1298
Hpy8I GTNNAC 4 cut(s) 115, 826, 848, 1142
HpyAV CCTTC 1 cut(s) 77
HpyCH4III ACNGT 2 cut(s) 793, 1304
HpyCH4IV ACGT 3 cut(s) 494, 832, 993
HpyCH4V TGCA 3 cut(s) 157, 267, 312
HpyF10VI GCNNNNNNNGC 3 cut(s) 69, 518, 1095
HpyF3I CTNAG 3 cut(s) 329, 1085, 1316
HpySE526I ACGT 3 cut(s) 494, 832, 993
Hsp92II CATG 6 cut(s) 367, 811, 895, 1075, 1198, 1255
Ksp22I TGATCA 1 cut(s) 94
KspI CCGCGG 1 cut(s) 839
Kzo9I GATC 3 cut(s) 94, 813, 1157
LmnI GCTCC 2 cut(s) 109, 132
Lsp1109I GCAGC 4 cut(s) 524, 891, 1033, 1307
LweI GCATC 3 cut(s) 144, 166, 288
MaeI CTAG 2 cut(s) 230, 1184
MaeII ACGT 3 cut(s) 494, 832, 993
MaeIII GTNAC 1 cut(s) 634
MalI GATC 3 cut(s) 96, 815, 1159
MboI GATC 3 cut(s) 94, 813, 1157
MboII GAAGA 5 cut(s) 39, 124, 565, 941, 1051
MhlI GDGCHC 1 cut(s) 1026
MlsI TGGCCA 2 cut(s) 63, 450
MluCI AATT 7 cut(s) 80, 307, 504, 554, 1001, 1259, 1327
MluNI TGGCCA 2 cut(s) 63, 450
MlyI GAGTC 2 cut(s) 769, 1257
MmeI TCCRAC 4 cut(s) 77, 204, 595, 736
Mox20I TGGCCA 2 cut(s) 63, 450
Mph1103I ATGCAT 1 cut(s) 159
MscI TGGCCA 2 cut(s) 63, 450
MseI TTAA 4 cut(s) 189, 354, 591, 1330
MslI CAYNNNNRTG 1 cut(s) 267
Msp20I TGGCCA 2 cut(s) 63, 450
MspA1I CMGCKG 2 cut(s) 838, 904
MspCI CTTAAG 1 cut(s) 188
MspI CCGG 2 cut(s) 234, 1163
MspR9I CCNGG 1 cut(s) 60
MvaI CCWGG 1 cut(s) 60
MvnI CGCG 1 cut(s) 838
MwoI GCNNNNNNNGC 3 cut(s) 69, 518, 1095
NdeI CATATG 2 cut(s) 755, 1228
NdeII GATC 3 cut(s) 94, 813, 1157
NheI GCTAGC 1 cut(s) 229
NlaIII CATG 6 cut(s) 367, 811, 895, 1075, 1198, 1255
NlaIV GGNNCC 3 cut(s) 105, 410, 1310
NmuCI GTSAC 1 cut(s) 634
NsiI ATGCAT 1 cut(s) 159
NspV TTCGAA 1 cut(s) 1263
PaeR7I CTCGAG 1 cut(s) 297
PcsI WCGNNNNNNNCGW 1 cut(s) 609
PfeI GAWTC 4 cut(s) 600, 618, 874, 909
PflMI CCANNNNNTGG 1 cut(s) 59
PkrI GCNGC 5 cut(s) 514, 775, 906, 1048, 1322
PleI GAGTC 2 cut(s) 768, 1256
PpsI GAGTC 2 cut(s) 768, 1256
Ppu21I YACGTR 1 cut(s) 994
PpuMI RGGWCCY 1 cut(s) 1309
Psp5II RGGWCCY 1 cut(s) 1309
Psp6I CCWGG 1 cut(s) 58
PspGI CCWGG 1 cut(s) 58
PspN4I GGNNCC 3 cut(s) 105, 410, 1310
PspPI GGNCC 3 cut(s) 208, 409, 1309
PspPPI RGGWCCY 1 cut(s) 1309
PspXI VCTCGAGB 1 cut(s) 297
RsaI GTAC 4 cut(s) 790, 806, 992, 1083
RsaNI GTAC 4 cut(s) 789, 805, 991, 1082
RseI CAYNNNNRTG 1 cut(s) 267
SacII CCGCGG 1 cut(s) 839
SaqAI TTAA 4 cut(s) 189, 354, 591, 1330
SatI GCNGC 5 cut(s) 513, 774, 905, 1047, 1321
Sau3AI GATC 3 cut(s) 94, 813, 1157
Sau96I GGNCC 3 cut(s) 208, 409, 1309
SchI GAGTC 2 cut(s) 769, 1257
ScrFI CCNGG 1 cut(s) 60
SduI GDGCHC 1 cut(s) 1026
SfaNI GCATC 3 cut(s) 144, 166, 288
Sfr274I CTCGAG 1 cut(s) 297
Sfr303I CCGCGG 1 cut(s) 839
SfuI TTCGAA 1 cut(s) 1263
SgrBI CCGCGG 1 cut(s) 839
SinI GGWCC 2 cut(s) 409, 1309
SlaI CTCGAG 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 267
SmlI CTYRAG 2 cut(s) 188, 297
SmoI CTYRAG 2 cut(s) 188, 297
Sse9I AATT 7 cut(s) 80, 307, 504, 554, 1001, 1259, 1327
SsiI CCGC 9 cut(s) 227, 747, 773, 836, 838, 902, 921, 1079, 1323
SspMI CTAG 2 cut(s) 230, 1184
StyD4I CCNGG 1 cut(s) 58
StyI CCWWGG 2 cut(s) 27, 1203
TaaI ACNGT 2 cut(s) 793, 1304
TaiI ACGT 3 cut(s) 497, 835, 996
TaqI TCGA 7 cut(s) 238, 291, 298, 378, 603, 763, 1263
TasI AATT 7 cut(s) 80, 307, 504, 554, 1001, 1259, 1327
TatI WGTACW 1 cut(s) 804
TauI GCSGC 1 cut(s) 776
TfiI GAWTC 4 cut(s) 600, 618, 874, 909
Tru1I TTAA 4 cut(s) 189, 354, 591, 1330
Tru9I TTAA 4 cut(s) 189, 354, 591, 1330
TscAI CASTG 4 cut(s) 175, 434, 828, 1173
TseFI GTSAC 1 cut(s) 634
TseI GCWGC 4 cut(s) 512, 904, 1046, 1320
Tsp45I GTSAC 1 cut(s) 634
TspRI CASTG 4 cut(s) 175, 434, 828, 1173
Van91I CCANNNNNTGG 1 cut(s) 59
Vha464I CTTAAG 1 cut(s) 188
VpaK11BI GGWCC 2 cut(s) 409, 1309
XapI RAATTY 2 cut(s) 504, 1259
XhoI CTCGAG 1 cut(s) 297
XmiI GTMKAC 1 cut(s) 114
XspI CTAG 2 cut(s) 230, 1184
Zsp2I ATGCAT 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.