RchiOBHm_Chr4g0431811

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000622
Physical Location & Seq
Reverse (-)
55944035 .. 55945372
1338 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40045

Sequence Viewer

Length: 1338 bp
ATGAAGGTTGAAGTAGAAGTAATCTCCAAGGAGATTATCACCCCATCTTCTCCAACCCCTGCTCATTTTCGCCATTACCAGCTCTCCTCCTTTGATCAATTAGCTCCCCCAGTCTATAACCCTTTGGTCCTCTTCTACGAATTCAATGACAAGACAGTGCCCAACATTACTGAAATATCCAGCCACCTCAAAAAGTCCCTAGCCGAAGTCTTAACCGTTTTCTACCCATTAGCCGGACGAATCAAACACGACGACTGGCTTGTTGATTGCAATGATGAGGGCATTCCCTACCTTGAAGCTCACGTCAATTGCAAACTCTCTGACTTCCTCAAGAATCCGATCCCTGATCAACTTAGCAAGTTCATGCCATTTGAACTTGATGATCATATTGGTAAGGAGTTGGTCCTAGGGGTCCAGCTCAGCATCTTTGAATGTGGAGGATTTGCAATTGGTCAATGCATCTCTCACAGGCTTGCCGATGGGTCATCTTATTTCATGTTCAGCAAAACTTGGGCGGCCATAGCCCATGGCGAGTCCAACATAGAGCATCCAGACTTTCTGTCAGCAACACGTTTTCCACTGAAGGATGTTATGGCATATGATCCAAGCACTGAAATCTCCAGGAATAAAGTCACAAAGAGGTTTTTATTCAGTGCCTCTATGATAGAGGCTCTCAGAGCAAAGTATGGGGAAAGCGCTGTAGGCATAGAAGAAAACCAGAAACGCCCATCACGTGTCGATGCTTTAACGGCTTTCATATGGAGTCGATATATGGCCAATACCAATAAGATTACAGGACCTCATGATGAGAAGTTGTATAGGATCGCCCATGCTGTGAACCTACGCCCAAGGTTTGATCCACCATTGCCACAACATTCTTTTGGAAACATTTACCGTGGTGCCACGTCAGGTCCCTTGCTAAGTACTGGGGATGCATGCAGTGCATATGGTCGGGCTATGAGGGAAGTACGAGAAGAAATAAACAAGATCGACAATGACTGCGTCAAAGGACTACAGCAGGGTGCTGAGCAGTCTGGGCTCCCCTTCAATGAGAATACTGGTAGGCTAACCGAACTAGAACTGGTTACGTTGGGCTTCAGTAGCTATTGCAGGTTTCCAGTGTATGATAATGATTTCGGTTGGGGAAGGCCTGCGTGGGTGTTCTCAACACCATTAACCTTCAAGAACGTAGCAACTTTCATGGACACCAAAGAGGGTGATGGAATAGAGGTATACATCAGTTTGGAGGAGCAACTCATGGCTAAATTTGAAACTGATGCTGAGTTCTTGGCCCCTATGTGTCTCCAAGTGGGCGCTGAAGAGCCCTTTGGTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

445

Amino Acids

50.0

Weight (kDa)

5.27

Isoelectric Point (pI)

43.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 4 - 429 4e-81 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24710 FvH4_4g24720 FvH4_4g24770 FvH4_4g24771 FvH4_4g24772 FvH4_5g39380
malus_domestica MD10G1199500.v1.1 MD13G1109100.v1.1 MD13G1109200.v1.1 MD13G1114400.v1.1 MD13G1114800.v1.1 MD16G1108800.v1.1
prunus_persica Prupe.1G235900_v2.0.a1 Prupe.1G236600_v2.0.a1 Prupe.1G236700_v2.0.a1 Prupe.1G236800_v2.0.a1 Prupe.4G142000_v2.0.a1
pyrus_communis pycom10g17180 pycom13g09460 pycom13g09930 pycom13g09940
rosa_chinensis RchiOBHm_Chr3g0490281 RchiOBHm_Chr3g0490291 RchiOBHm_Chr3g0490301 RchiOBHm_Chr4g0417441 RchiOBHm_Chr4g0417461 RchiOBHm_Chr4g0431811 RchiOBHm_Chr4g0431821 RchiOBHm_Chr4g0431851
rosa_laevigata RLG00000006882 RLG00000006886 RLG00000006887 RLG00000007905 RLG00000007911 RLG00000007913 RLG00000029538
rosa_multiflora Rmu_sc0001459.1_g000008 Rmu_sc0001459.1_g000011 Rmu_sc0002489.1_g000020 Rmu_sc0002489.1_g000024 Rmu_sc0002489.1_g000027 Rmu_sc0002489.1_g000028 Rmu_sc0002489.1_g000029 Rmu_sc0004142.1_g000001 Rmu_sc0004142.1_g000024 Rmu_sc0011237.1_g000018 Rmu_sc0031439.1_g000001 Rmu_sc0031677.1_g000001 Rmu_ssc0000361.1_g000005
rosa_roxburghii Rroxscaffold_3G00262760 Rroxscaffold_4G00317310 Rroxscaffold_5G00361150 Rroxscaffold_5G00373190 Rroxscaffold_5G00373210 Rroxscaffold_5G00373220 Rroxscaffold_5G00373240
rosa_rugosa Rorug01G0111100 Rorug04G0252400 Rorug04G0252400 Rorug04G0252400 Rorug04G0252600
rosa_samantha Rh1BG102300 Rh3AG292200 Rh3AG292300 Rh4AG309100 Rh4AG309300 Rh4AG309500 Rh6BG200900
rosa_wichuraiana Rw0G002990 Rw0G003000 Rw0G019170 Rw3G025890 Rw3G025900 Rw4G017830 Rw4G026810 Rw4G026870 Rw4G026880 Rw4G026920 Rw6G017190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1101
AccB1I GGYRCC 1 cut(s) 899
AccI GTMKAC 1 cut(s) 1233
AciI CCGC 1 cut(s) 515
AclWI GGATC 4 cut(s) 334, 596, 830, 851
AcoI YGGCCR 2 cut(s) 516, 774
AcsI RAATTY 2 cut(s) 140, 1265
AcuI CTGAAG 3 cut(s) 602, 1081, 1338
AcvI CACGTG 1 cut(s) 734
AfaI GTAC 2 cut(s) 925, 969
AfeI AGCGCT 1 cut(s) 697
AfiI CCNNNNNNNGG 1 cut(s) 233
AflIII ACRYGT 2 cut(s) 569, 733
AgsI TTSAA 8 cut(s) 11, 145, 296, 374, 431, 1048, 1183, 1271
AhdI GACNNNNNGTC 1 cut(s) 559
AjiI CACGTC 2 cut(s) 304, 906
AjnI CCWGG 1 cut(s) 620
AjuI GAANNNNNNNTTGG 1 cut(s) 1311
AluBI AGCT 5 cut(s) 82, 104, 299, 418, 1104
AluI AGCT 5 cut(s) 82, 104, 299, 418, 1104
Alw26I GTCTC 1 cut(s) 1307
AlwI GGATC 4 cut(s) 334, 596, 830, 851
Aor51HI AGCGCT 1 cut(s) 697
AoxI GGCC 4 cut(s) 516, 774, 1148, 1290
ApoI RAATTY 2 cut(s) 140, 1265
AspA2I CCTAGG 1 cut(s) 406
AspLEI GCGC 2 cut(s) 698, 1316
AspS9I GGNCC 6 cut(s) 127, 403, 412, 797, 911, 1291
AsuHPI GGTGA 2 cut(s) 31, 1229
AvaII GGWCC 5 cut(s) 127, 403, 412, 797, 911
AvrII CCTAGG 1 cut(s) 406
BaeGI GKGCMC 1 cut(s) 162
BalI TGGCCA 1 cut(s) 776
BanI GGYRCC 1 cut(s) 899
BanII GRGCYC 2 cut(s) 1041, 1326
BbrPI CACGTG 1 cut(s) 734
BccI CCATC 4 cut(s) 52, 473, 736, 1214
BceAI ACGGC 1 cut(s) 765
BciT130I CCWGG 1 cut(s) 622
BclI TGATCA 3 cut(s) 94, 346, 382
BcoDI GTCTC 1 cut(s) 1307
BfaI CTAG 3 cut(s) 200, 407, 1076
BfmI CTRYAG 2 cut(s) 699, 1013
BfoI RGCGCY 2 cut(s) 699, 1317
BfuAI ACCTGC 1 cut(s) 1101
BisI GCNGC 1 cut(s) 516
BlnI CCTAGG 1 cut(s) 406
BlpI GCTNAGC 2 cut(s) 419, 1026
BlsI GCNGC 1 cut(s) 517
BmcAI AGTACT 1 cut(s) 925
Bme1390I CCNGG 1 cut(s) 622
Bme18I GGWCC 5 cut(s) 127, 403, 412, 797, 911
BmeRI GACNNNNNGTC 1 cut(s) 559
BmgBI CACGTC 2 cut(s) 304, 906
BmgT120I GGNCC 6 cut(s) 127, 403, 412, 797, 911, 1291
BmiI GGNNCC 5 cut(s) 413, 901, 913, 1040, 1293
BmrFI CCNGG 1 cut(s) 622
BmrI ACTGGG 2 cut(s) 104, 936
BmsI GCATC 6 cut(s) 432, 468, 556, 730, 922, 1267
BmuI ACTGGG 2 cut(s) 104, 936
BpmI CTGGAG 1 cut(s) 604
Bpu1102I GCTNAGC 2 cut(s) 419, 1026
BpuEI CTTGAG 1 cut(s) 314
BsaAI YACGTR 1 cut(s) 734
BsaJI CCNNGG 5 cut(s) 27, 406, 526, 848, 895
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bsc4I CCNNNNNNNGG 1 cut(s) 233
Bse1I ACTGG 6 cut(s) 110, 260, 931, 1063, 1086, 1118
Bse3DI GCAATG 2 cut(s) 277, 863
BseBI CCWGG 1 cut(s) 622
BseDI CCNNGG 5 cut(s) 27, 406, 526, 848, 895
BseGI GGATG 3 cut(s) 547, 592, 937
BseLI CCNNNNNNNGG 1 cut(s) 233
BseMI GCAATG 2 cut(s) 277, 863
BseMII CTCAG 4 cut(s) 433, 688, 1017, 1272
BseNI ACTGG 6 cut(s) 110, 260, 931, 1063, 1086, 1118
BseRI GAGGAG 2 cut(s) 76, 1262
BseSI GKGCMC 1 cut(s) 162
BshFI GGCC 4 cut(s) 518, 776, 1150, 1292
BshNI GGYRCC 1 cut(s) 899
BsiSI CCGG 1 cut(s) 234
BslFI GGGAC 2 cut(s) 181, 897
BslI CCNNNNNNNGG 1 cut(s) 233
BsmAI GTCTC 1 cut(s) 1307
BsmFI GGGAC 2 cut(s) 181, 897
BsmI GAATGC 1 cut(s) 282
BsnI GGCC 4 cut(s) 518, 776, 1150, 1292
Bsp1286I GDGCHC 3 cut(s) 162, 1041, 1326
Bsp143I GATC 8 cut(s) 94, 339, 346, 382, 601, 822, 856, 987
Bsp1720I GCTNAGC 2 cut(s) 419, 1026
Bsp19I CCATGG 1 cut(s) 526
BspACI CCGC 1 cut(s) 515
BspANI GGCC 4 cut(s) 518, 776, 1150, 1292
BspCNI CTCAG 4 cut(s) 432, 687, 1018, 1273
BspHI TCATGA 1 cut(s) 802
BspLI GGNNCC 5 cut(s) 413, 901, 913, 1040, 1293
BspMI ACCTGC 1 cut(s) 1101
BspPI GGATC 4 cut(s) 334, 596, 830, 851
BspQI GCTCTTC 1 cut(s) 1314
BspT107I GGYRCC 1 cut(s) 899
BsrDI GCAATG 2 cut(s) 277, 863
BsrI ACTGG 6 cut(s) 110, 260, 931, 1063, 1086, 1118
BssECI CCNNGG 5 cut(s) 27, 406, 526, 848, 895
BssMI GATC 8 cut(s) 94, 339, 346, 382, 601, 822, 856, 987
BssNAI GTATAC 1 cut(s) 1234
BssT1I CCWWGG 4 cut(s) 27, 406, 526, 848
Bst1107I GTATAC 1 cut(s) 1234
Bst2UI CCWGG 1 cut(s) 622
Bst4CI ACNGT 3 cut(s) 157, 217, 896
Bst6I CTCTTC 2 cut(s) 137, 1314
BstAPI GCANNNNNTGC 1 cut(s) 941
BstBAI YACGTR 1 cut(s) 734
BstC8I GCNNGC 3 cut(s) 474, 937, 1152
BstDEI CTNAG 6 cut(s) 353, 419, 674, 920, 1026, 1281
BstDSI CCRYGG 2 cut(s) 526, 895
BstF5I GGATG 3 cut(s) 547, 592, 937
BstH2I RGCGCY 2 cut(s) 699, 1317
BstHHI GCGC 2 cut(s) 698, 1316
BstKTI GATC 8 cut(s) 97, 342, 349, 385, 604, 825, 859, 990
BstMAI GTCTC 1 cut(s) 1307
BstMBI GATC 8 cut(s) 94, 339, 346, 382, 601, 822, 856, 987
BstMWI GCNNNNNNNGC 7 cut(s) 521, 677, 702, 749, 941, 1036, 1101
BstNI CCWGG 1 cut(s) 622
BstNSI RCATGY 1 cut(s) 939
BstSCI CCNGG 1 cut(s) 620
BstSFI CTRYAG 2 cut(s) 699, 1013
BstSLI GKGCMC 1 cut(s) 162
BstZ17I GTATAC 1 cut(s) 1234
BsuRI GGCC 4 cut(s) 518, 776, 1150, 1292
BtgI CCRYGG 2 cut(s) 526, 895
BtrI CACGTC 2 cut(s) 304, 906
BtsCI GGATG 3 cut(s) 547, 592, 937
BtsI GCAGTG 1 cut(s) 946
BtsIMutI CAGTG 6 cut(s) 162, 578, 609, 658, 946, 1125
BveI ACCTGC 1 cut(s) 1101
Cac8I GCNNGC 3 cut(s) 474, 937, 1152
CciI TCATGA 1 cut(s) 802
CfoI GCGC 2 cut(s) 698, 1316
Cfr13I GGNCC 6 cut(s) 127, 403, 412, 797, 911, 1291
CseI GACGC 1 cut(s) 991
Csp6I GTAC 2 cut(s) 924, 968
CviAII CATG 8 cut(s) 364, 496, 527, 803, 830, 936, 1201, 1258
CviQI GTAC 2 cut(s) 924, 968
DdeI CTNAG 6 cut(s) 353, 419, 674, 920, 1026, 1281
DpnI GATC 8 cut(s) 96, 341, 348, 384, 603, 824, 858, 989
DpnII GATC 8 cut(s) 94, 339, 346, 382, 601, 822, 856, 987
DriI GACNNNNNGTC 1 cut(s) 559
EaeI YGGCCR 2 cut(s) 516, 774
Eam1104I CTCTTC 2 cut(s) 137, 1314
Eam1105I GACNNNNNGTC 1 cut(s) 559
EarI CTCTTC 2 cut(s) 137, 1314
Eco130I CCWWGG 4 cut(s) 27, 406, 526, 848
Eco147I AGGCCT 1 cut(s) 1150
Eco24I GRGCYC 2 cut(s) 1041, 1326
Eco47I GGWCC 5 cut(s) 127, 403, 412, 797, 911
Eco47III AGCGCT 1 cut(s) 697
Eco57I CTGAAG 3 cut(s) 602, 1081, 1338
Eco72I CACGTG 1 cut(s) 734
EcoO109I RGGNCCY 2 cut(s) 797, 911
EcoRI GAATTC 1 cut(s) 140
EcoRII CCWGG 1 cut(s) 620
EcoT14I CCWWGG 4 cut(s) 27, 406, 526, 848
EcoT22I ATGCAT 2 cut(s) 461, 937
EcoT38I GRGCYC 2 cut(s) 1041, 1326
ErhI CCWWGG 4 cut(s) 27, 406, 526, 848
FaeI CATG 8 cut(s) 367, 499, 530, 806, 833, 939, 1204, 1261
FaqI GGGAC 2 cut(s) 181, 897
FatI CATG 8 cut(s) 363, 495, 526, 802, 829, 935, 1200, 1257
FauNDI CATATG 3 cut(s) 598, 758, 946
FbaI TGATCA 3 cut(s) 94, 346, 382
FblI GTMKAC 1 cut(s) 1233
Fnu4HI GCNGC 1 cut(s) 516
FokI GGATG 3 cut(s) 534, 599, 944
FriOI GRGCYC 2 cut(s) 1041, 1326
Fsp4HI GCNGC 1 cut(s) 516
FspBI CTAG 3 cut(s) 200, 407, 1076
GlaI GCGC 2 cut(s) 697, 1315
GluI GCNGC 1 cut(s) 516
GsuI CTGGAG 1 cut(s) 604
HaeII RGCGCY 2 cut(s) 699, 1317
HaeIII GGCC 4 cut(s) 518, 776, 1150, 1292
HapII CCGG 1 cut(s) 234
HgaI GACGC 1 cut(s) 991
HhaI GCGC 2 cut(s) 698, 1316
Hin1II CATG 8 cut(s) 367, 499, 530, 806, 833, 939, 1204, 1261
Hin6I GCGC 2 cut(s) 696, 1314
HinP1I GCGC 2 cut(s) 696, 1314
HinfI GANTC 4 cut(s) 240, 334, 533, 763
HpaII CCGG 1 cut(s) 234
HphI GGTGA 2 cut(s) 31, 1229
Hpy166II GTNNAC 2 cut(s) 838, 1234
Hpy188I TCNGA 3 cut(s) 322, 339, 677
Hpy188III TCNNGA 4 cut(s) 331, 551, 803, 1183
Hpy8I GTNNAC 2 cut(s) 838, 1234
Hpy99I CGWCG 1 cut(s) 254
HpyAV CCTTC 4 cut(s) 577, 1054, 1140, 1189
HpyCH4III ACNGT 3 cut(s) 157, 217, 896
HpyCH4IV ACGT 6 cut(s) 303, 571, 733, 905, 1088, 1188
HpyCH4V TGCA 8 cut(s) 270, 312, 446, 459, 935, 939, 944, 1110
HpyF10VI GCNNNNNNNGC 7 cut(s) 521, 677, 702, 749, 941, 1036, 1101
HpyF3I CTNAG 6 cut(s) 353, 419, 674, 920, 1026, 1281
HpySE526I ACGT 6 cut(s) 303, 571, 733, 905, 1088, 1188
Hsp92II CATG 8 cut(s) 367, 499, 530, 806, 833, 939, 1204, 1261
HspAI GCGC 2 cut(s) 696, 1314
Ksp22I TGATCA 3 cut(s) 94, 346, 382
Kzo9I GATC 8 cut(s) 94, 339, 346, 382, 601, 822, 856, 987
LguI GCTCTTC 1 cut(s) 1314
LmnI GCTCC 3 cut(s) 109, 1044, 1249
LweI GCATC 6 cut(s) 432, 468, 556, 730, 922, 1267
MaeI CTAG 3 cut(s) 200, 407, 1076
MaeII ACGT 6 cut(s) 303, 571, 733, 905, 1088, 1188
MaeIII GTNAC 2 cut(s) 631, 1084
MalI GATC 8 cut(s) 96, 341, 348, 384, 603, 824, 858, 989
MboI GATC 8 cut(s) 94, 339, 346, 382, 601, 822, 856, 987
MboII GAAGA 5 cut(s) 39, 124, 722, 986, 1331
MfeI CAATTG 2 cut(s) 307, 447
MhlI GDGCHC 3 cut(s) 162, 1041, 1326
MlsI TGGCCA 1 cut(s) 776
MluCI AATT 5 cut(s) 98, 140, 307, 447, 1265
MluNI TGGCCA 1 cut(s) 776
MlyI GAGTC 2 cut(s) 542, 772
MmeI TCCRAC 2 cut(s) 77, 561
Mox20I TGGCCA 1 cut(s) 776
Mph1103I ATGCAT 2 cut(s) 461, 937
MscI TGGCCA 1 cut(s) 776
MseI TTAA 4 cut(s) 212, 746, 1175, 1336
Msp20I TGGCCA 1 cut(s) 776
MspI CCGG 1 cut(s) 234
MspR9I CCNGG 1 cut(s) 622
MunI CAATTG 2 cut(s) 307, 447
Mva1269I GAATGC 1 cut(s) 282
MvaI CCWGG 1 cut(s) 622
MwoI GCNNNNNNNGC 7 cut(s) 521, 677, 702, 749, 941, 1036, 1101
NcoI CCATGG 1 cut(s) 526
NdeI CATATG 3 cut(s) 598, 758, 946
NdeII GATC 8 cut(s) 94, 339, 346, 382, 601, 822, 856, 987
NlaIII CATG 8 cut(s) 367, 499, 530, 806, 833, 939, 1204, 1261
NlaIV GGNNCC 5 cut(s) 413, 901, 913, 1040, 1293
NmuCI GTSAC 1 cut(s) 631
NsiI ATGCAT 2 cut(s) 461, 937
NspI RCATGY 1 cut(s) 939
PaeI GCATGC 1 cut(s) 939
PagI TCATGA 1 cut(s) 802
PceI AGGCCT 1 cut(s) 1150
PciSI GCTCTTC 1 cut(s) 1314
PcsI WCGNNNNNNNCGW 1 cut(s) 730
PctI GAATGC 1 cut(s) 282
PfeI GAWTC 2 cut(s) 240, 334
PflFI GACNNNGTC 1 cut(s) 1001
PfoI TCCNGGA 1 cut(s) 620
PkrI GCNGC 1 cut(s) 517
PleI GAGTC 2 cut(s) 541, 771
PmaCI CACGTG 1 cut(s) 734
PmlI CACGTG 1 cut(s) 734
PpsI GAGTC 2 cut(s) 541, 771
Ppu21I YACGTR 1 cut(s) 734
PpuMI RGGWCCY 2 cut(s) 797, 911
Psp5II RGGWCCY 2 cut(s) 797, 911
Psp6I CCWGG 1 cut(s) 620
PspCI CACGTG 1 cut(s) 734
PspGI CCWGG 1 cut(s) 620
PspN4I GGNNCC 5 cut(s) 413, 901, 913, 1040, 1293
PspPI GGNCC 6 cut(s) 127, 403, 412, 797, 911, 1291
PspPPI RGGWCCY 2 cut(s) 797, 911
PsyI GACNNNGTC 1 cut(s) 1001
RsaI GTAC 2 cut(s) 925, 969
RsaNI GTAC 2 cut(s) 924, 968
SapI GCTCTTC 1 cut(s) 1314
SaqAI TTAA 4 cut(s) 212, 746, 1175, 1336
SatI GCNGC 1 cut(s) 516
Sau3AI GATC 8 cut(s) 94, 339, 346, 382, 601, 822, 856, 987
Sau96I GGNCC 6 cut(s) 127, 403, 412, 797, 911, 1291
ScaI AGTACT 1 cut(s) 925
SchI GAGTC 2 cut(s) 542, 772
ScrFI CCNGG 1 cut(s) 622
SduI GDGCHC 3 cut(s) 162, 1041, 1326
SfaNI GCATC 6 cut(s) 432, 468, 556, 730, 922, 1267
SfcI CTRYAG 2 cut(s) 699, 1013
SinI GGWCC 5 cut(s) 127, 403, 412, 797, 911
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
SphI GCATGC 1 cut(s) 939
Sse9I AATT 5 cut(s) 98, 140, 307, 447, 1265
SseBI AGGCCT 1 cut(s) 1150
SsiI CCGC 1 cut(s) 515
SspMI CTAG 3 cut(s) 200, 407, 1076
StuI AGGCCT 1 cut(s) 1150
StyD4I CCNGG 1 cut(s) 620
StyI CCWWGG 4 cut(s) 27, 406, 526, 848
TaaI ACNGT 3 cut(s) 157, 217, 896
TaiI ACGT 6 cut(s) 306, 574, 736, 908, 1091, 1191
TaqI TCGA 3 cut(s) 738, 766, 990
TasI AATT 5 cut(s) 98, 140, 307, 447, 1265
TatI WGTACW 1 cut(s) 923
TauI GCSGC 1 cut(s) 518
TfiI GAWTC 2 cut(s) 240, 334
Tru1I TTAA 4 cut(s) 212, 746, 1175, 1336
Tru9I TTAA 4 cut(s) 212, 746, 1175, 1336
TscAI CASTG 6 cut(s) 162, 585, 616, 658, 946, 1125
TseFI GTSAC 1 cut(s) 631
Tsp45I GTSAC 1 cut(s) 631
TspDTI ATGAA 5 cut(s) 17, 352, 484, 745, 1189
TspRI CASTG 6 cut(s) 162, 585, 616, 658, 946, 1125
Tth111I GACNNNGTC 1 cut(s) 1001
VpaK11BI GGWCC 5 cut(s) 127, 403, 412, 797, 911
XapI RAATTY 2 cut(s) 140, 1265
XceI RCATGY 1 cut(s) 939
XmaJI CCTAGG 1 cut(s) 406
XmiI GTMKAC 1 cut(s) 1233
XspI CTAG 3 cut(s) 200, 407, 1076
ZrmI AGTACT 1 cut(s) 925
Zsp2I ATGCAT 2 cut(s) 461, 937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.