Rh6BG200900

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000622
Physical Location & Seq
Forward (+)
37848090 .. 37849821
1732 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG200900.1

Sequence Viewer

Length: 1269 bp
ATGAAGTTAGAAGCTGAAGTCATCTCCAAACAGATTATCACTCCTTCTCCAACCCCTGAACATCTTCGCCATTACCAGTTCTCATTTCTTGATCAAATATCTCCCCCGGTCTATAACCCTTTGATCCTCTTTTATGAATTCAATGCCGAAACTCAACCCAACATCACTGAAATATCCAACCACCTGAAGACCTCCTTAGCTAAAGTCTTAACCCTATTCTACCCACTAGCCGGACGAATCGAAGGCAACCAACATGTGGATTGCAATGACAATGGTATACCCTACCTTGAAGCTCAAGTGAACTGCAAACTCTCAAATGTTCTCGACAATCCATTACCCGGAGAACTCAACAAACTCATGCCATTTGAGTTGGACGATGTGGCAAACAAATTTGCCCTTGGTGTCCAGCTCAACATCTTTCAATGTGGTGGTTTTGCTATTGCTCAGTGCCTTTCTCACAAGCTCGCAGACGGGTTATCATACTTCACATTCAGCAAAACTTGGGCGGCCACCGCCCGTGACGAACCCAAAATAGCACATCCACAGTTCATTTCGGCCACACTATTCCCTCCCAAGAATTTAAGTGGATTTGATGCACGCACTGGTATCACAAGAAACAAAGTGACAAAACGATTTGTGTTCAGTGCCTCTAACATAGAGGAGCTCAGAAAAGTTCAAGCACATAAGGCCTTAACACGACCTACACGAGTTGAGGCCTTATCTTCTTTCATCTTGAACCGATTTATGGAGGTTACCAAAGACATGGGATCAGTTGACAAGTTTTACAGGGTGGTTCATGCTGTGAACTTGCGGCCAAGGTTCGACCCACCACTACTGCACCATTCCTTTGGGAACCTTTACCGAGTCGCCGTAACAGCTCCGTTACAAATAAGTTCCAAGGAACCATATCATGGTATACGAGAAGCGATAAGTAAGATCGACAATGAGTACGTGAGAAAACTACAGAAAGGTGATGAGCATTTGAGTTGCATCAAGACCTATGCTAAAAGTTTTGCTAAAGGCGAGCAGGTTATACTTAGCTTCAGTAGTTATTGCTGGTTTCCTCTGTACGATAATGATTTTGGTTGGGGGAGGCCTACGTGGGTGGGGTCGCCGGCACTGATGTTCAAGAACCTAGTGAACCTCATGGATACCAAAGAGGGTGATGGAATTGAGGTTTATATTAGCTTGGAGGAGGAAGTCATGACTAAGTTTGAGAGCGATGTGGAGCTCTTAGCTTATGTGTCTCCAAGTGCGGTGCTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

422

Amino Acids

47.71

Weight (kDa)

6.65

Isoelectric Point (pI)

39.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 6 - 411 4.2e-72 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24710 FvH4_4g24720 FvH4_4g24770 FvH4_4g24771 FvH4_4g24772 FvH4_5g39380
malus_domestica MD10G1199500.v1.1 MD13G1109100.v1.1 MD13G1109200.v1.1 MD13G1114400.v1.1 MD13G1114800.v1.1 MD16G1108800.v1.1
prunus_persica Prupe.1G235900_v2.0.a1 Prupe.1G236600_v2.0.a1 Prupe.1G236700_v2.0.a1 Prupe.1G236800_v2.0.a1 Prupe.4G142000_v2.0.a1
pyrus_communis pycom10g17180 pycom13g09460 pycom13g09930 pycom13g09940
rosa_chinensis RchiOBHm_Chr3g0490281 RchiOBHm_Chr3g0490291 RchiOBHm_Chr3g0490301 RchiOBHm_Chr4g0417441 RchiOBHm_Chr4g0417461 RchiOBHm_Chr4g0431811 RchiOBHm_Chr4g0431821 RchiOBHm_Chr4g0431851
rosa_laevigata RLG00000006882 RLG00000006886 RLG00000006887 RLG00000007905 RLG00000007911 RLG00000007913 RLG00000029538
rosa_multiflora Rmu_sc0001459.1_g000008 Rmu_sc0001459.1_g000011 Rmu_sc0002489.1_g000020 Rmu_sc0002489.1_g000024 Rmu_sc0002489.1_g000027 Rmu_sc0002489.1_g000028 Rmu_sc0002489.1_g000029 Rmu_sc0004142.1_g000001 Rmu_sc0004142.1_g000024 Rmu_sc0011237.1_g000018 Rmu_sc0031439.1_g000001 Rmu_sc0031677.1_g000001 Rmu_ssc0000361.1_g000005
rosa_roxburghii Rroxscaffold_3G00262760 Rroxscaffold_4G00317310 Rroxscaffold_5G00361150 Rroxscaffold_5G00373190 Rroxscaffold_5G00373210 Rroxscaffold_5G00373220 Rroxscaffold_5G00373240
rosa_rugosa Rorug01G0111100 Rorug04G0252400 Rorug04G0252400 Rorug04G0252400 Rorug04G0252600
rosa_samantha Rh1BG102300 Rh3AG292200 Rh3AG292300 Rh4AG309100 Rh4AG309300 Rh4AG309500 Rh6BG200900
rosa_wichuraiana Rw0G002990 Rw0G003000 Rw0G019170 Rw3G025890 Rw3G025900 Rw4G017830 Rw4G026810 Rw4G026870 Rw4G026880 Rw4G026920 Rw6G017190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1018
AccB7I CCANNNNNTGG 2 cut(s) 256, 911
AccI GTMKAC 2 cut(s) 277, 916
AciI CCGC 4 cut(s) 506, 513, 811, 1256
AclWI GGATC 2 cut(s) 118, 775
AcoI YGGCCR 3 cut(s) 507, 555, 812
AcsI RAATTY 3 cut(s) 137, 389, 577
AcuI CTGAAG 3 cut(s) 36, 206, 1027
AfaI GTAC 2 cut(s) 950, 1070
AfiI CCNNNNNNNGG 5 cut(s) 230, 256, 338, 745, 911
AflIII ACRYGT 1 cut(s) 253
AgsI TTSAA 6 cut(s) 142, 290, 422, 677, 736, 1129
Alw21I GWGCWC 2 cut(s) 666, 1233
Alw26I GTCTC 1 cut(s) 1251
AlwI GGATC 2 cut(s) 118, 775
AoxI GGCC 6 cut(s) 507, 555, 687, 714, 812, 1094
ApoI RAATTY 3 cut(s) 137, 389, 577
Asp700I GAANNNNTTC 1 cut(s) 63
AsuC2I CCSGG 2 cut(s) 107, 339
AsuHPI GGTGA 2 cut(s) 983, 1175
BanII GRGCYC 2 cut(s) 666, 1233
BauI CACGAG 1 cut(s) 705
BbsI GAAGAC 1 cut(s) 194
Bbv12I GWGCWC 2 cut(s) 666, 1233
BccI CCATC 1 cut(s) 1160
BceAI ACGGC 1 cut(s) 854
BciVI GTATCC 1 cut(s) 1144
BclI TGATCA 1 cut(s) 91
BcnI CCSGG 2 cut(s) 107, 339
BcoDI GTCTC 1 cut(s) 1251
BfaI CTAG 2 cut(s) 227, 1136
BfmI CTRYAG 1 cut(s) 962
BfuAI ACCTGC 1 cut(s) 1018
BfuI GTATCC 1 cut(s) 1144
BisI GCNGC 2 cut(s) 507, 812
BlsI GCNGC 2 cut(s) 508, 813
Bme1390I CCNGG 2 cut(s) 107, 339
BmiI GGNNCC 2 cut(s) 854, 903
BmrFI CCNGG 2 cut(s) 107, 339
BmsI GCATC 2 cut(s) 583, 999
BpiI GAAGAC 1 cut(s) 194
Bpu10I CCTNAGC 1 cut(s) 196
BpuEI CTTGAG 1 cut(s) 279
BpuMI CCSGG 2 cut(s) 107, 339
BsaAI YACGTR 2 cut(s) 952, 1101
BsaJI CCNNGG 4 cut(s) 105, 397, 815, 897
BsaXI ACNNNNNCTCC 2 cut(s) 31, 61
Bsc4I CCNNNNNNNGG 5 cut(s) 230, 256, 338, 745, 911
Bse118I RCCGGY 1 cut(s) 1114
Bse1I ACTGG 2 cut(s) 76, 607
Bse3DI GCAATG 1 cut(s) 271
BseDI CCNNGG 4 cut(s) 105, 397, 815, 897
BseGI GGATG 1 cut(s) 538
BseLI CCNNNNNNNGG 5 cut(s) 230, 256, 338, 745, 911
BseMI GCAATG 1 cut(s) 271
BseMII CTCAG 2 cut(s) 458, 679
BseNI ACTGG 2 cut(s) 76, 607
BseRI GAGGAG 2 cut(s) 674, 1208
BsgI GTGCAG 1 cut(s) 821
BshFI GGCC 6 cut(s) 509, 557, 689, 716, 814, 1096
BsiHKAI GWGCWC 2 cut(s) 666, 1233
BsiSI CCGG 4 cut(s) 107, 231, 339, 1115
BslI CCNNNNNNNGG 5 cut(s) 230, 256, 338, 745, 911
BsmAI GTCTC 1 cut(s) 1251
BsnI GGCC 6 cut(s) 509, 557, 689, 716, 814, 1096
Bsp1286I GDGCHC 2 cut(s) 666, 1233
Bsp143I GATC 4 cut(s) 91, 123, 767, 936
BspACI CCGC 4 cut(s) 506, 513, 811, 1256
BspANI GGCC 6 cut(s) 509, 557, 689, 716, 814, 1096
BspCNI CTCAG 2 cut(s) 457, 678
BspHI TCATGA 1 cut(s) 1203
BspLI GGNNCC 2 cut(s) 854, 903
BspMI ACCTGC 1 cut(s) 1018
BspPI GGATC 2 cut(s) 118, 775
BsrDI GCAATG 1 cut(s) 271
BsrFI RCCGGY 1 cut(s) 1114
BsrI ACTGG 2 cut(s) 76, 607
BssAI RCCGGY 1 cut(s) 1114
BssECI CCNNGG 4 cut(s) 105, 397, 815, 897
BssMI GATC 4 cut(s) 91, 123, 767, 936
BssNAI GTATAC 2 cut(s) 278, 917
BssSI CACGAG 1 cut(s) 705
BssT1I CCWWGG 3 cut(s) 397, 815, 897
Bst1107I GTATAC 2 cut(s) 278, 917
Bst2BI CACGAG 1 cut(s) 705
Bst4CI ACNGT 1 cut(s) 546
BstBAI YACGTR 2 cut(s) 952, 1101
BstC8I GCNNGC 4 cut(s) 465, 598, 1025, 1116
BstDEI CTNAG 6 cut(s) 196, 444, 665, 1037, 1209, 1234
BstEII GGTNACC 1 cut(s) 751
BstF5I GGATG 1 cut(s) 538
BstKTI GATC 4 cut(s) 94, 126, 770, 939
BstMAI GTCTC 1 cut(s) 1251
BstMBI GATC 4 cut(s) 91, 123, 767, 936
BstMWI GCNNNNNNNGC 3 cut(s) 512, 686, 875
BstNSI RCATGY 1 cut(s) 257
BstPI GGTNACC 1 cut(s) 751
BstSCI CCNGG 2 cut(s) 105, 337
BstSFI CTRYAG 1 cut(s) 962
BstV2I GAAGAC 1 cut(s) 194
BstXI CCANNNNNNTGG 2 cut(s) 763, 848
BstZ17I GTATAC 2 cut(s) 278, 917
BsuI GTATCC 1 cut(s) 1144
BsuRI GGCC 6 cut(s) 509, 557, 689, 716, 814, 1096
BtgZI GCGATG 1 cut(s) 1236
BtsCI GGATG 1 cut(s) 538
BtsIMutI CAGTG 5 cut(s) 165, 452, 600, 649, 1118
BveI ACCTGC 1 cut(s) 1018
Cac8I GCNNGC 4 cut(s) 465, 598, 1025, 1116
CciI TCATGA 1 cut(s) 1203
Cfr10I RCCGGY 1 cut(s) 1114
Csp6I GTAC 2 cut(s) 949, 1069
CviAII CATG 7 cut(s) 254, 358, 763, 797, 911, 1147, 1204
CviQI GTAC 2 cut(s) 949, 1069
DdeI CTNAG 6 cut(s) 196, 444, 665, 1037, 1209, 1234
DpnI GATC 4 cut(s) 93, 125, 769, 938
DpnII GATC 4 cut(s) 91, 123, 767, 936
EaeI YGGCCR 3 cut(s) 507, 555, 812
Ecl136II GAGCTC 2 cut(s) 664, 1231
Eco130I CCWWGG 3 cut(s) 397, 815, 897
Eco147I AGGCCT 3 cut(s) 689, 716, 1096
Eco24I GRGCYC 2 cut(s) 666, 1233
Eco53kI GAGCTC 2 cut(s) 664, 1231
Eco57I CTGAAG 3 cut(s) 36, 206, 1027
Eco91I GGTNACC 1 cut(s) 751
EcoICRI GAGCTC 2 cut(s) 664, 1231
EcoO65I GGTNACC 1 cut(s) 751
EcoRI GAATTC 1 cut(s) 137
EcoT14I CCWWGG 3 cut(s) 397, 815, 897
EcoT38I GRGCYC 2 cut(s) 666, 1233
ErhI CCWWGG 3 cut(s) 397, 815, 897
FaeI CATG 7 cut(s) 257, 361, 766, 800, 914, 1150, 1207
FalI AAGNNNNNCTT 2 cut(s) 179, 211
FatI CATG 7 cut(s) 253, 357, 762, 796, 910, 1146, 1203
FbaI TGATCA 1 cut(s) 91
FblI GTMKAC 2 cut(s) 277, 916
Fnu4HI GCNGC 2 cut(s) 507, 812
FokI GGATG 1 cut(s) 525
FriOI GRGCYC 2 cut(s) 666, 1233
Fsp4HI GCNGC 2 cut(s) 507, 812
FspBI CTAG 2 cut(s) 227, 1136
GluI GCNGC 2 cut(s) 507, 812
HaeIII GGCC 6 cut(s) 509, 557, 689, 716, 814, 1096
HapII CCGG 4 cut(s) 107, 231, 339, 1115
Hin1II CATG 7 cut(s) 257, 361, 766, 800, 914, 1150, 1207
HincII GTYRAC 1 cut(s) 775
HindII GTYRAC 1 cut(s) 775
HinfI GANTC 2 cut(s) 237, 864
HpaII CCGG 4 cut(s) 107, 231, 339, 1115
HphI GGTGA 2 cut(s) 983, 1175
Hpy166II GTNNAC 6 cut(s) 278, 301, 775, 805, 917, 1141
Hpy188I TCNGA 1 cut(s) 668
Hpy188III TCNNGA 6 cut(s) 89, 323, 733, 994, 1129, 1204
Hpy8I GTNNAC 6 cut(s) 278, 301, 775, 805, 917, 1141
HpyAV CCTTC 2 cut(s) 54, 236
HpyCH4III ACNGT 1 cut(s) 546
HpyCH4IV ACGT 2 cut(s) 951, 1100
HpyCH4V TGCA 5 cut(s) 264, 306, 596, 838, 990
HpyF10VI GCNNNNNNNGC 3 cut(s) 512, 686, 875
HpyF3I CTNAG 6 cut(s) 196, 444, 665, 1037, 1209, 1234
HpySE526I ACGT 2 cut(s) 951, 1100
Hsp92II CATG 7 cut(s) 257, 361, 766, 800, 914, 1150, 1207
KroI GCCGGC 1 cut(s) 1114
KroNI GCCGGC 1 cut(s) 1116
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 4 cut(s) 91, 123, 767, 936
LmnI GCTCC 3 cut(s) 661, 883, 1228
LweI GCATC 2 cut(s) 583, 999
MaeI CTAG 2 cut(s) 227, 1136
MaeII ACGT 2 cut(s) 951, 1100
MaeIII GTNAC 5 cut(s) 518, 622, 751, 871, 882
MalI GATC 4 cut(s) 93, 125, 769, 938
MboI GATC 4 cut(s) 91, 123, 767, 936
MboII GAAGA 3 cut(s) 56, 199, 714
MhlI GDGCHC 2 cut(s) 666, 1233
MluCI AATT 4 cut(s) 137, 389, 577, 1170
MlyI GAGTC 1 cut(s) 873
MmeI TCCRAC 3 cut(s) 74, 201, 351
MroNI GCCGGC 1 cut(s) 1114
MroXI GAANNNNTTC 1 cut(s) 63
MseI TTAA 3 cut(s) 209, 581, 692
MspI CCGG 4 cut(s) 107, 231, 339, 1115
MspR9I CCNGG 2 cut(s) 107, 339
MwoI GCNNNNNNNGC 3 cut(s) 512, 686, 875
NaeI GCCGGC 1 cut(s) 1116
NciI CCSGG 2 cut(s) 107, 339
NdeII GATC 4 cut(s) 91, 123, 767, 936
NgoMIV GCCGGC 1 cut(s) 1114
NlaIII CATG 7 cut(s) 257, 361, 766, 800, 914, 1150, 1207
NlaIV GGNNCC 2 cut(s) 854, 903
NmuCI GTSAC 2 cut(s) 518, 622
NspI RCATGY 1 cut(s) 257
PagI TCATGA 1 cut(s) 1203
PceI AGGCCT 3 cut(s) 689, 716, 1096
PciI ACATGT 1 cut(s) 253
PcsI WCGNNNNNNNCGW 1 cut(s) 703
PdiI GCCGGC 1 cut(s) 1116
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 1 cut(s) 237
PflMI CCANNNNNTGG 2 cut(s) 256, 911
PkrI GCNGC 2 cut(s) 508, 813
PleI GAGTC 1 cut(s) 872
PpsI GAGTC 1 cut(s) 872
Ppu21I YACGTR 2 cut(s) 952, 1101
PscI ACATGT 1 cut(s) 253
Psp124BI GAGCTC 2 cut(s) 666, 1233
PspEI GGTNACC 1 cut(s) 751
PspN4I GGNNCC 2 cut(s) 854, 903
RsaI GTAC 2 cut(s) 950, 1070
RsaNI GTAC 2 cut(s) 949, 1069
SacI GAGCTC 2 cut(s) 666, 1233
SaqAI TTAA 3 cut(s) 209, 581, 692
SatI GCNGC 2 cut(s) 507, 812
Sau3AI GATC 4 cut(s) 91, 123, 767, 936
SchI GAGTC 1 cut(s) 873
ScrFI CCNGG 2 cut(s) 107, 339
SduI GDGCHC 2 cut(s) 666, 1233
SfaNI GCATC 2 cut(s) 583, 999
SfcI CTRYAG 1 cut(s) 962
SmlI CTYRAG 1 cut(s) 294
SmoI CTYRAG 1 cut(s) 294
Sse9I AATT 4 cut(s) 137, 389, 577, 1170
SseBI AGGCCT 3 cut(s) 689, 716, 1096
SsiI CCGC 4 cut(s) 506, 513, 811, 1256
SspMI CTAG 2 cut(s) 227, 1136
SstI GAGCTC 2 cut(s) 666, 1233
StuI AGGCCT 3 cut(s) 689, 716, 1096
StyD4I CCNGG 2 cut(s) 105, 337
StyI CCWWGG 3 cut(s) 397, 815, 897
TaaI ACNGT 1 cut(s) 546
TaiI ACGT 2 cut(s) 954, 1103
TaqI TCGA 4 cut(s) 240, 324, 822, 939
TasI AATT 4 cut(s) 137, 389, 577, 1170
TauI GCSGC 2 cut(s) 509, 814
TfiI GAWTC 1 cut(s) 237
Tru1I TTAA 3 cut(s) 209, 581, 692
Tru9I TTAA 3 cut(s) 209, 581, 692
TscAI CASTG 5 cut(s) 172, 452, 607, 649, 1125
TseFI GTSAC 2 cut(s) 518, 622
Tsp45I GTSAC 2 cut(s) 518, 622
TspDTI ATGAA 5 cut(s) 17, 150, 538, 718, 785
TspGWI ACGGA 1 cut(s) 870
TspRI CASTG 5 cut(s) 172, 452, 607, 649, 1125
Van91I CCANNNNNTGG 2 cut(s) 256, 911
XapI RAATTY 3 cut(s) 137, 389, 577
XceI RCATGY 1 cut(s) 257
XmiI GTMKAC 2 cut(s) 277, 916
XmnI GAANNNNTTC 1 cut(s) 63
XspI CTAG 2 cut(s) 227, 1136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.