Rroxscaffold_5G00361150

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000622
Physical Location & Seq
Forward (+)
41758550 .. 41759464
915 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00361150.1

Sequence Viewer

Length: 915 bp
ATGCCATTTGAACTGGACGATGTGGCAAACAAATTTGCCCTTGGTATCCAGCTCAACATCTTTCAATGTGGTGGTTTTGCTATTGCTCAGTGCCTTTCTCACAAGCTCGCAGATGGGTTATCATACTTCACGTTCAGCAAAACTTGGGCCGCCACTGCCCGTGGCGAACCCAACATAGCACATCCGCAGTTCATTTCCGCCACACTATTCCCTCCCAAGAATTTAAGTGGATTTGATGGGCGCACAGGTATCACAAGAAACAAAGTGACAAAACGCTTTGTGTTTAGTGCCTCTAACATAGAGGAGCTCAGAAAAGTTCAAGCACATAAGAACTTAACACGCCCTTCACGTGTTGAGGCCTTATCTTCTTTCATCTTGAGCCGATTTATGGAGGCTACCAAAGATATTGGATCAGCTGACAAGTTTTACAGGGTGGTTCATGCTGTGAACTTGCGGCCGAGGTTCGACCCACCACTGCCGCACCATTCTTTTGGGAACCTTTACCGAATAGCCATGACAGCACCGTTACAAATAAGTTCCAGGGAACCATATCATGGTATACGAAAAGTGATAAGCAAGATCGACAATGAGTACGTGAGAAAACTGCAGAAGGGTGATGAGCATTTGAGTTTCATCAAGACCCAAGCTGAACTTTTTGCTAAAGGCGAGCAGGTTATAATTAGCTTCAGTAGTTATTGCTGGTTTCCTCTGTACGATAACGATTTTGGTTGGGGGAGGCCTACGTGGGTGGGGTTACCGGCATTGACGTTCAAGAACATAGTGAACTTCATGGATACCAAAGAGGGTGGTGGAATTGAGGCTTATATTAGCTTGGGGGAGGAAGTCATGACTAAATTTGAGAGCGATGTGGAGCTCTTAGCTTATGTGTCTCCAAGTGCAGTGCTGAACGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

304

Amino Acids

34.2

Weight (kDa)

8.99

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 14 - 292 5.7e-41 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24710 FvH4_4g24720 FvH4_4g24770 FvH4_4g24771 FvH4_4g24772 FvH4_5g39380
malus_domestica MD10G1199500.v1.1 MD13G1109100.v1.1 MD13G1109200.v1.1 MD13G1114400.v1.1 MD13G1114800.v1.1 MD16G1108800.v1.1
prunus_persica Prupe.1G235900_v2.0.a1 Prupe.1G236600_v2.0.a1 Prupe.1G236700_v2.0.a1 Prupe.1G236800_v2.0.a1 Prupe.4G142000_v2.0.a1
pyrus_communis pycom10g17180 pycom13g09460 pycom13g09930 pycom13g09940
rosa_chinensis RchiOBHm_Chr3g0490281 RchiOBHm_Chr3g0490291 RchiOBHm_Chr3g0490301 RchiOBHm_Chr4g0417441 RchiOBHm_Chr4g0417461 RchiOBHm_Chr4g0431811 RchiOBHm_Chr4g0431821 RchiOBHm_Chr4g0431851
rosa_laevigata RLG00000006882 RLG00000006886 RLG00000006887 RLG00000007905 RLG00000007911 RLG00000007913 RLG00000029538
rosa_multiflora Rmu_sc0001459.1_g000008 Rmu_sc0001459.1_g000011 Rmu_sc0002489.1_g000020 Rmu_sc0002489.1_g000024 Rmu_sc0002489.1_g000027 Rmu_sc0002489.1_g000028 Rmu_sc0002489.1_g000029 Rmu_sc0004142.1_g000001 Rmu_sc0004142.1_g000024 Rmu_sc0011237.1_g000018 Rmu_sc0031439.1_g000001 Rmu_sc0031677.1_g000001 Rmu_ssc0000361.1_g000005
rosa_roxburghii Rroxscaffold_3G00262760 Rroxscaffold_4G00317310 Rroxscaffold_5G00361150 Rroxscaffold_5G00373190 Rroxscaffold_5G00373210 Rroxscaffold_5G00373220 Rroxscaffold_5G00373240
rosa_rugosa Rorug01G0111100 Rorug04G0252400 Rorug04G0252400 Rorug04G0252400 Rorug04G0252600
rosa_samantha Rh1BG102300 Rh3AG292200 Rh3AG292300 Rh4AG309100 Rh4AG309300 Rh4AG309500 Rh6BG200900
rosa_wichuraiana Rw0G002990 Rw0G003000 Rw0G019170 Rw3G025890 Rw3G025900 Rw4G017830 Rw4G026810 Rw4G026870 Rw4G026880 Rw4G026920 Rw6G017190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 677
Acc36I ACCTGC 1 cut(s) 661
AccB7I CCANNNNNTGG 1 cut(s) 554
AccI GTMKAC 1 cut(s) 559
AciI CCGC 5 cut(s) 150, 185, 198, 454, 479
AclI AACGTT 1 cut(s) 909
AclWI GGATC 1 cut(s) 418
AcoI YGGCCR 1 cut(s) 455
AcsI RAATTY 3 cut(s) 32, 220, 854
AcuI CTGAAG 1 cut(s) 670
AcvI CACGTG 1 cut(s) 350
AfaI GTAC 2 cut(s) 593, 713
AfiI CCNNNNNNNGG 2 cut(s) 388, 554
AflIII ACRYGT 1 cut(s) 349
AgsI TTSAA 4 cut(s) 11, 65, 320, 772
AjnI CCWGG 1 cut(s) 539
AluBI AGCT 9 cut(s) 52, 106, 307, 416, 647, 684, 831, 874, 881
AluI AGCT 9 cut(s) 52, 106, 307, 416, 647, 684, 831, 874, 881
Alw21I GWGCWC 2 cut(s) 309, 876
Alw26I GTCTC 1 cut(s) 894
AlwI GGATC 1 cut(s) 418
AoxI GGCC 4 cut(s) 147, 357, 455, 737
ApoI RAATTY 3 cut(s) 32, 220, 854
AspLEI GCGC 1 cut(s) 243
AspS9I GGNCC 1 cut(s) 147
AsuHPI GGTGA 1 cut(s) 626
BanII GRGCYC 2 cut(s) 309, 876
BbrPI CACGTG 1 cut(s) 350
Bbv12I GWGCWC 2 cut(s) 309, 876
BccI CCATC 2 cut(s) 107, 230
BciT130I CCWGG 1 cut(s) 541
BciVI GTATCC 2 cut(s) 56, 787
BcoDI GTCTC 1 cut(s) 894
BfmI CTRYAG 1 cut(s) 605
BfuAI ACCTGC 1 cut(s) 661
BfuI GTATCC 2 cut(s) 56, 787
BisI GCNGC 3 cut(s) 150, 455, 479
BlsI GCNGC 3 cut(s) 151, 456, 480
Bme1390I CCNGG 1 cut(s) 541
BmgT120I GGNCC 1 cut(s) 147
BmiI GGNNCC 2 cut(s) 497, 546
BmrFI CCNGG 1 cut(s) 541
BpuEI CTTGAG 1 cut(s) 397
BsaAI YACGTR 3 cut(s) 350, 595, 744
BsaJI CCNNGG 4 cut(s) 40, 160, 458, 540
Bsc4I CCNNNNNNNGG 2 cut(s) 388, 554
Bse118I RCCGGY 1 cut(s) 757
Bse1I ACTGG 1 cut(s) 18
BseBI CCWGG 1 cut(s) 541
BseDI CCNNGG 4 cut(s) 40, 160, 458, 540
BseGI GGATG 1 cut(s) 181
BseLI CCNNNNNNNGG 2 cut(s) 388, 554
BseMII CTCAG 2 cut(s) 101, 322
BseNI ACTGG 1 cut(s) 18
BseRI GAGGAG 1 cut(s) 317
BseX3I CGGCCG 1 cut(s) 455
Bsh1285I CGRYCG 1 cut(s) 458
BshFI GGCC 4 cut(s) 149, 359, 457, 739
BsiEI CGRYCG 1 cut(s) 458
BsiHKAI GWGCWC 2 cut(s) 309, 876
BsiSI CCGG 1 cut(s) 758
BslI CCNNNNNNNGG 2 cut(s) 388, 554
BsmAI GTCTC 1 cut(s) 894
BsnI GGCC 4 cut(s) 149, 359, 457, 739
Bsp1286I GDGCHC 2 cut(s) 309, 876
Bsp143I GATC 2 cut(s) 410, 579
BspACI CCGC 5 cut(s) 150, 185, 198, 454, 479
BspANI GGCC 4 cut(s) 149, 359, 457, 739
BspCNI CTCAG 2 cut(s) 100, 321
BspHI TCATGA 1 cut(s) 846
BspLI GGNNCC 2 cut(s) 497, 546
BspMAI CTGCAG 1 cut(s) 609
BspMI ACCTGC 1 cut(s) 661
BspPI GGATC 1 cut(s) 418
BsrFI RCCGGY 1 cut(s) 757
BsrI ACTGG 1 cut(s) 18
BssAI RCCGGY 1 cut(s) 757
BssECI CCNNGG 4 cut(s) 40, 160, 458, 540
BssMI GATC 2 cut(s) 410, 579
BssNAI GTATAC 1 cut(s) 560
BssT1I CCWWGG 1 cut(s) 40
Bst1107I GTATAC 1 cut(s) 560
Bst2UI CCWGG 1 cut(s) 541
Bst4CI ACNGT 1 cut(s) 525
BstBAI YACGTR 3 cut(s) 350, 595, 744
BstC8I GCNNGC 2 cut(s) 108, 668
BstDEI CTNAG 3 cut(s) 87, 308, 877
BstDSI CCRYGG 1 cut(s) 160
BstEII GGTNACC 1 cut(s) 753
BstF5I GGATG 1 cut(s) 181
BstHHI GCGC 1 cut(s) 243
BstKTI GATC 2 cut(s) 413, 582
BstMAI GTCTC 1 cut(s) 894
BstMBI GATC 2 cut(s) 410, 579
BstMCI CGRYCG 1 cut(s) 458
BstMWI GCNNNNNNNGC 2 cut(s) 155, 518
BstNI CCWGG 1 cut(s) 541
BstPI GGTNACC 1 cut(s) 753
BstSCI CCNGG 1 cut(s) 539
BstSFI CTRYAG 1 cut(s) 605
BstXI CCANNNNNNTGG 1 cut(s) 491
BstZ17I GTATAC 1 cut(s) 560
BstZI CGGCCG 1 cut(s) 455
BsuI GTATCC 2 cut(s) 56, 787
BsuRI GGCC 4 cut(s) 149, 359, 457, 739
BtgI CCRYGG 1 cut(s) 160
BtgZI GCGATG 1 cut(s) 879
BtsCI GGATG 1 cut(s) 181
BtsI GCAGTG 3 cut(s) 153, 473, 906
BtsIMutI CAGTG 4 cut(s) 95, 153, 473, 906
BveI ACCTGC 1 cut(s) 661
Cac8I GCNNGC 2 cut(s) 108, 668
CciI TCATGA 1 cut(s) 846
CfoI GCGC 1 cut(s) 243
Cfr10I RCCGGY 1 cut(s) 757
Cfr13I GGNCC 1 cut(s) 147
Csp6I GTAC 2 cut(s) 592, 712
CspCI CAANNNNNGTGG 2 cut(s) 787, 822
CviAII CATG 5 cut(s) 440, 514, 554, 790, 847
CviQI GTAC 2 cut(s) 592, 712
DdeI CTNAG 3 cut(s) 87, 308, 877
DpnI GATC 2 cut(s) 412, 581
DpnII GATC 2 cut(s) 410, 579
EaeI YGGCCR 1 cut(s) 455
EagI CGGCCG 1 cut(s) 455
EciI GGCGGA 1 cut(s) 187
Ecl136II GAGCTC 2 cut(s) 307, 874
EclXI CGGCCG 1 cut(s) 455
Eco130I CCWWGG 1 cut(s) 40
Eco147I AGGCCT 2 cut(s) 359, 739
Eco24I GRGCYC 2 cut(s) 309, 876
Eco52I CGGCCG 1 cut(s) 455
Eco53kI GAGCTC 2 cut(s) 307, 874
Eco57I CTGAAG 1 cut(s) 670
Eco72I CACGTG 1 cut(s) 350
Eco91I GGTNACC 1 cut(s) 753
EcoICRI GAGCTC 2 cut(s) 307, 874
EcoO65I GGTNACC 1 cut(s) 753
EcoRII CCWGG 1 cut(s) 539
EcoT14I CCWWGG 1 cut(s) 40
EcoT38I GRGCYC 2 cut(s) 309, 876
ErhI CCWWGG 1 cut(s) 40
FaeI CATG 5 cut(s) 443, 517, 557, 793, 850
FalI AAGNNNNNCTT 2 cut(s) 636, 668
FatI CATG 5 cut(s) 439, 513, 553, 789, 846
FblI GTMKAC 1 cut(s) 559
Fnu4HI GCNGC 3 cut(s) 150, 455, 479
FokI GGATG 1 cut(s) 168
FriOI GRGCYC 2 cut(s) 309, 876
Fsp4HI GCNGC 3 cut(s) 150, 455, 479
GlaI GCGC 1 cut(s) 242
GluI GCNGC 3 cut(s) 150, 455, 479
HaeIII GGCC 4 cut(s) 149, 359, 457, 739
HapII CCGG 1 cut(s) 758
HhaI GCGC 1 cut(s) 243
Hin1II CATG 5 cut(s) 443, 517, 557, 793, 850
Hin6I GCGC 1 cut(s) 241
HinP1I GCGC 1 cut(s) 241
HpaII CCGG 1 cut(s) 758
HphI GGTGA 1 cut(s) 626
Hpy166II GTNNAC 3 cut(s) 448, 560, 784
Hpy188I TCNGA 1 cut(s) 311
Hpy188III TCNNGA 4 cut(s) 376, 637, 772, 847
Hpy8I GTNNAC 3 cut(s) 448, 560, 784
HpyAV CCTTC 2 cut(s) 354, 604
HpyCH4III ACNGT 1 cut(s) 525
HpyCH4IV ACGT 6 cut(s) 131, 349, 594, 743, 767, 909
HpyCH4V TGCA 2 cut(s) 607, 899
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 518
HpyF3I CTNAG 3 cut(s) 87, 308, 877
HpySE526I ACGT 6 cut(s) 131, 349, 594, 743, 767, 909
Hsp92II CATG 5 cut(s) 443, 517, 557, 793, 850
HspAI GCGC 1 cut(s) 241
Kzo9I GATC 2 cut(s) 410, 579
LmnI GCTCC 2 cut(s) 304, 871
LpnPI CCDG 8 cut(s) 62, 231, 415, 526, 553, 656, 685, 771
MaeII ACGT 6 cut(s) 131, 349, 594, 743, 767, 909
MaeIII GTNAC 3 cut(s) 265, 525, 753
MalI GATC 2 cut(s) 412, 581
MboI GATC 2 cut(s) 410, 579
MboII GAAGA 1 cut(s) 357
MhlI GDGCHC 2 cut(s) 309, 876
MluCI AATT 5 cut(s) 32, 220, 678, 813, 854
MseI TTAA 3 cut(s) 224, 335, 913
MspA1I CMGCKG 1 cut(s) 416
MspI CCGG 1 cut(s) 758
MspR9I CCNGG 1 cut(s) 541
MvaI CCWGG 1 cut(s) 541
MwoI GCNNNNNNNGC 2 cut(s) 155, 518
NdeII GATC 2 cut(s) 410, 579
NlaIII CATG 5 cut(s) 443, 517, 557, 793, 850
NlaIV GGNNCC 2 cut(s) 497, 546
NmeAIII GCCGAG 1 cut(s) 483
NmuCI GTSAC 1 cut(s) 265
PagI TCATGA 1 cut(s) 846
PceI AGGCCT 2 cut(s) 359, 739
PcsI WCGNNNNNNNCGW 1 cut(s) 346
PflMI CCANNNNNTGG 1 cut(s) 554
PkrI GCNGC 3 cut(s) 151, 456, 480
PmaCI CACGTG 1 cut(s) 350
PmlI CACGTG 1 cut(s) 350
Ppu21I YACGTR 3 cut(s) 350, 595, 744
PsiI TTATAA 1 cut(s) 677
Psp124BI GAGCTC 2 cut(s) 309, 876
Psp1406I AACGTT 1 cut(s) 909
Psp6I CCWGG 1 cut(s) 539
PspCI CACGTG 1 cut(s) 350
PspEI GGTNACC 1 cut(s) 753
PspGI CCWGG 1 cut(s) 539
PspN4I GGNNCC 2 cut(s) 497, 546
PspPI GGNCC 1 cut(s) 147
PstI CTGCAG 1 cut(s) 609
PvuII CAGCTG 1 cut(s) 416
RsaI GTAC 2 cut(s) 593, 713
RsaNI GTAC 2 cut(s) 592, 712
SacI GAGCTC 2 cut(s) 309, 876
SaqAI TTAA 3 cut(s) 224, 335, 913
SatI GCNGC 3 cut(s) 150, 455, 479
Sau3AI GATC 2 cut(s) 410, 579
Sau96I GGNCC 1 cut(s) 147
ScrFI CCNGG 1 cut(s) 541
SduI GDGCHC 2 cut(s) 309, 876
SfcI CTRYAG 1 cut(s) 605
SmlI CTYRAG 1 cut(s) 376
SmoI CTYRAG 1 cut(s) 376
Sse9I AATT 5 cut(s) 32, 220, 678, 813, 854
SseBI AGGCCT 2 cut(s) 359, 739
SsiI CCGC 5 cut(s) 150, 185, 198, 454, 479
SstI GAGCTC 2 cut(s) 309, 876
StuI AGGCCT 2 cut(s) 359, 739
StyD4I CCNGG 1 cut(s) 539
StyI CCWWGG 1 cut(s) 40
TaaI ACNGT 1 cut(s) 525
TaiI ACGT 6 cut(s) 134, 352, 597, 746, 770, 912
TaqI TCGA 2 cut(s) 465, 582
TasI AATT 5 cut(s) 32, 220, 678, 813, 854
TauI GCSGC 3 cut(s) 152, 457, 481
Tru1I TTAA 3 cut(s) 224, 335, 913
Tru9I TTAA 3 cut(s) 224, 335, 913
TscAI CASTG 4 cut(s) 95, 160, 480, 906
TseFI GTSAC 1 cut(s) 265
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 5 cut(s) 181, 361, 428, 622, 778
TspRI CASTG 4 cut(s) 95, 160, 480, 906
Van91I CCANNNNNTGG 1 cut(s) 554
XapI RAATTY 3 cut(s) 32, 220, 854
XmiI GTMKAC 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.