FvH4_4g24770

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000622
Physical Location & Seq
Reverse (-)
26782334 .. 26783623
1290 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g24770.t1

Sequence Viewer

Length: 1290 bp
ATGGGTATAATGAAGTTTGAAGCCGAAGTGATCTCCAAGCATATTATCAAGCCTGCTTCTCCAACCCCTGACCATCTTCGCCATTACCAGTTCTCTTTTCTTGATCAAATATCTCCCCCGGTCTATAACCCTTTGCTTCTCTTTTATGAATTCAATGCCAAAACTCAACCCAACATCACTGAAATATCCAACCACCTGAAGACCTCCTTAGCCAAAGTCCTTAGCCTCTACTACCCACTAGCCGGACGATTCAAAGACAACCAACATGTGGATTGCAATGACAATGGTGTACCCTACCTTGAAGCTCAAGTCAACTGCAAACTTTCTGATGTTCTCAACAATCCAATCCCTGGAGAACTCAACAAGCTCATGCCATTTGAGCTCGACGATGTGGCAAGCAAATATCCCCTTGGCGTCCAGCTCAACGTGTTTCAATGTGGTGGTTTTGCTATTGCTCAATGCCTTACTCATAAGCTCGCAGATGGGTTGTCATACTTCACGTTCAGCAAAACTTGGGCCGCCACCGCCGGTGGCGAACCTAAAATAGCGCAGCCGCAGTTCATTTCCGCCACACTATTCCCTCCCAAAAATTTAAGCGGATACGATGCGCGCACCGGCATCACCAGAAACAAAGTAACAAAAAGATTTGTGTTTAGTGCCATTAACATAGAGGAGCTCAGAAAAGTTCAGGCGAATAAGGCATCAGCACACCCTTCGCGTGTTGAGGCCTTATCGTCTTTCATCTTAAGCCGATTTGTGGAGGCTACCAAAGACATTGGATCGGCTGAAAAGTTTTACAGGGTGGTTCATGCTGTGAACTTGCGGCCAAGGTTCGAACCACCACTGCCACACCATTCTTTTGGGAACCTTTACCGAGTTGCCATGACAGCTCCGTTACAAATAAGTTCCGGGGAACCATATCACGGTATACGTGAAGAGATAAGCAAGATCGACAATGCATACGTGAGAAAACTACAGAAAGGTGATGAGCATTTGAGCTCCATCAAGACGTATGCTAAGAGTTTTGCTAAAGGCGAGCAGGTTACAATCAGCTTCAGTAGTTATTGTAGGTTTCCTCTGTATGATAACGATTTTGGTTGGGGGAGGCCTACGTGGGTGGGATCGCCGGCACTGACGTTCAAGAACCTAGTGAACTTCATGGACACCAGAGAGGGTGGTGGAATTGAGGCGTATATTAGCTTGGAGGAGGAAGTCATGACAAAGTTTGAGAGCGATGTAGAGCTCTTAGCTTATATGTCTTCGAGTGCGGTGCTGAACCAAGCAACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

430

Amino Acids

48.04

Weight (kDa)

8.11

Isoelectric Point (pI)

40.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 8 - 415 3.7e-75 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24710 FvH4_4g24720 FvH4_4g24770 FvH4_4g24771 FvH4_4g24772 FvH4_5g39380
malus_domestica MD10G1199500.v1.1 MD13G1109100.v1.1 MD13G1109200.v1.1 MD13G1114400.v1.1 MD13G1114800.v1.1 MD16G1108800.v1.1
prunus_persica Prupe.1G235900_v2.0.a1 Prupe.1G236600_v2.0.a1 Prupe.1G236700_v2.0.a1 Prupe.1G236800_v2.0.a1 Prupe.4G142000_v2.0.a1
pyrus_communis pycom10g17180 pycom13g09460 pycom13g09930 pycom13g09940
rosa_chinensis RchiOBHm_Chr3g0490281 RchiOBHm_Chr3g0490291 RchiOBHm_Chr3g0490301 RchiOBHm_Chr4g0417441 RchiOBHm_Chr4g0417461 RchiOBHm_Chr4g0431811 RchiOBHm_Chr4g0431821 RchiOBHm_Chr4g0431851
rosa_laevigata RLG00000006882 RLG00000006886 RLG00000006887 RLG00000007905 RLG00000007911 RLG00000007913 RLG00000029538
rosa_multiflora Rmu_sc0001459.1_g000008 Rmu_sc0001459.1_g000011 Rmu_sc0002489.1_g000020 Rmu_sc0002489.1_g000024 Rmu_sc0002489.1_g000027 Rmu_sc0002489.1_g000028 Rmu_sc0002489.1_g000029 Rmu_sc0004142.1_g000001 Rmu_sc0004142.1_g000024 Rmu_sc0011237.1_g000018 Rmu_sc0031439.1_g000001 Rmu_sc0031677.1_g000001 Rmu_ssc0000361.1_g000005
rosa_roxburghii Rroxscaffold_3G00262760 Rroxscaffold_4G00317310 Rroxscaffold_5G00361150 Rroxscaffold_5G00373190 Rroxscaffold_5G00373210 Rroxscaffold_5G00373220 Rroxscaffold_5G00373240
rosa_rugosa Rorug01G0111100 Rorug04G0252400 Rorug04G0252400 Rorug04G0252400 Rorug04G0252600
rosa_samantha Rh1BG102300 Rh3AG292200 Rh3AG292300 Rh4AG309100 Rh4AG309300 Rh4AG309500 Rh6BG200900
rosa_wichuraiana Rw0G002990 Rw0G003000 Rw0G019170 Rw3G025890 Rw3G025900 Rw4G017830 Rw4G026810 Rw4G026870 Rw4G026880 Rw4G026920 Rw6G017190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1030
AccB7I CCANNNNNTGG 2 cut(s) 268, 350
AccI GTMKAC 1 cut(s) 928
AccII CGCG 2 cut(s) 610, 718
AciI CCGC 7 cut(s) 519, 525, 554, 567, 597, 823, 1268
AclWI GGATC 2 cut(s) 787, 1129
AcoI YGGCCR 1 cut(s) 824
AcsI RAATTY 2 cut(s) 149, 589
AcuI CTGAAG 2 cut(s) 218, 1039
AcyI GRCGYC 1 cut(s) 414
AfaI GTAC 1 cut(s) 291
AfiI CCNNNNNNNGG 5 cut(s) 242, 268, 350, 757, 923
AflII CTTAAG 1 cut(s) 745
AflIII ACRYGT 2 cut(s) 265, 426
AgsI TTSAA 6 cut(s) 20, 154, 253, 302, 434, 1141
AjnI CCWGG 1 cut(s) 349
Alw21I GWGCWC 4 cut(s) 384, 678, 1001, 1245
AlwI GGATC 2 cut(s) 787, 1129
AoxI GGCC 4 cut(s) 516, 726, 824, 1106
ApeKI GCWGC 1 cut(s) 550
ApoI RAATTY 2 cut(s) 149, 589
AspLEI GCGC 3 cut(s) 550, 610, 612
AspS9I GGNCC 1 cut(s) 516
AsuC2I CCSGG 2 cut(s) 119, 910
AsuHPI GGTGA 2 cut(s) 613, 995
AsuII TTCGAA 1 cut(s) 834
BanII GRGCYC 4 cut(s) 384, 678, 1001, 1245
BbsI GAAGAC 2 cut(s) 206, 1251
Bbv12I GWGCWC 4 cut(s) 384, 678, 1001, 1245
BbvI GCAGC 1 cut(s) 562
BccI CCATC 3 cut(s) 81, 476, 1010
BcgI CGANNNNNNTGC 4 cut(s) 696, 730, 1252, 1286
BciT130I CCWGG 1 cut(s) 351
BciVI GTATCC 1 cut(s) 593
BclI TGATCA 1 cut(s) 103
BcnI CCSGG 2 cut(s) 119, 910
BfaI CTAG 2 cut(s) 239, 1148
BfmI CTRYAG 1 cut(s) 974
BfrI CTTAAG 1 cut(s) 745
BfuAI ACCTGC 1 cut(s) 1030
BfuI GTATCC 1 cut(s) 593
BisI GCNGC 4 cut(s) 519, 551, 554, 824
BlsI GCNGC 4 cut(s) 520, 552, 555, 825
Bme1390I CCNGG 3 cut(s) 119, 351, 910
BmgT120I GGNCC 1 cut(s) 516
BmiI GGNNCC 2 cut(s) 866, 915
BmrFI CCNGG 3 cut(s) 119, 351, 910
BmsI GCATC 3 cut(s) 595, 627, 710
BpiI GAAGAC 2 cut(s) 206, 1251
BpmI CTGGAG 1 cut(s) 372
Bpu10I CCTNAGC 2 cut(s) 208, 221
Bpu14I TTCGAA 1 cut(s) 834
BpuEI CTTGAG 1 cut(s) 291
BpuMI CCSGG 2 cut(s) 119, 910
BsaAI YACGTR 3 cut(s) 932, 964, 1113
BsaHI GRCGYC 1 cut(s) 414
BsaJI CCNNGG 5 cut(s) 117, 349, 409, 827, 909
Bsc4I CCNNNNNNNGG 5 cut(s) 242, 268, 350, 757, 923
Bse118I RCCGGY 3 cut(s) 527, 614, 1126
Bse1I ACTGG 1 cut(s) 88
Bse3DI GCAATG 1 cut(s) 283
BseBI CCWGG 1 cut(s) 351
BseDI CCNNGG 5 cut(s) 117, 349, 409, 827, 909
BseLI CCNNNNNNNGG 5 cut(s) 242, 268, 350, 757, 923
BseMI GCAATG 1 cut(s) 283
BseMII CTCAG 1 cut(s) 691
BseNI ACTGG 1 cut(s) 88
BsePI GCGCGC 1 cut(s) 608
BseRI GAGGAG 2 cut(s) 686, 1220
BseXI GCAGC 1 cut(s) 562
Bsh1236I CGCG 2 cut(s) 610, 718
BshFI GGCC 4 cut(s) 518, 728, 826, 1108
BsiHKAI GWGCWC 4 cut(s) 384, 678, 1001, 1245
BsiSI CCGG 6 cut(s) 119, 243, 528, 615, 909, 1127
BslI CCNNNNNNNGG 5 cut(s) 242, 268, 350, 757, 923
BsnI GGCC 4 cut(s) 518, 728, 826, 1108
Bsp119I TTCGAA 1 cut(s) 834
Bsp1286I GDGCHC 4 cut(s) 384, 678, 1001, 1245
Bsp143I GATC 5 cut(s) 30, 103, 779, 948, 1121
BspACI CCGC 7 cut(s) 519, 525, 554, 567, 597, 823, 1268
BspANI GGCC 4 cut(s) 518, 728, 826, 1108
BspCNI CTCAG 1 cut(s) 690
BspFNI CGCG 2 cut(s) 610, 718
BspHI TCATGA 1 cut(s) 1215
BspLI GGNNCC 2 cut(s) 866, 915
BspMI ACCTGC 1 cut(s) 1030
BspPI GGATC 2 cut(s) 787, 1129
BspT104I TTCGAA 1 cut(s) 834
BspTI CTTAAG 1 cut(s) 745
BsrDI GCAATG 1 cut(s) 283
BsrFI RCCGGY 3 cut(s) 527, 614, 1126
BsrI ACTGG 1 cut(s) 88
BssAI RCCGGY 3 cut(s) 527, 614, 1126
BssECI CCNNGG 5 cut(s) 117, 349, 409, 827, 909
BssHII GCGCGC 1 cut(s) 608
BssMI GATC 5 cut(s) 30, 103, 779, 948, 1121
BssNAI GTATAC 1 cut(s) 929
BssNI GRCGYC 1 cut(s) 414
BssT1I CCWWGG 2 cut(s) 409, 827
Bst1107I GTATAC 1 cut(s) 929
Bst2UI CCWGG 1 cut(s) 351
Bst4CI ACNGT 1 cut(s) 926
Bst6I CTCTTC 1 cut(s) 930
BstACI GRCGYC 1 cut(s) 414
BstAFI CTTAAG 1 cut(s) 745
BstBAI YACGTR 3 cut(s) 932, 964, 1113
BstBI TTCGAA 1 cut(s) 834
BstC8I GCNNGC 6 cut(s) 54, 397, 477, 610, 1037, 1128
BstDEI CTNAG 5 cut(s) 208, 221, 677, 1017, 1246
BstFNI CGCG 2 cut(s) 610, 718
BstHHI GCGC 3 cut(s) 550, 610, 612
BstKTI GATC 5 cut(s) 33, 106, 782, 951, 1124
BstMBI GATC 5 cut(s) 30, 103, 779, 948, 1121
BstMWI GCNNNNNNNGC 4 cut(s) 379, 524, 698, 887
BstNI CCWGG 1 cut(s) 351
BstNSI RCATGY 1 cut(s) 269
BstSCI CCNGG 3 cut(s) 117, 349, 908
BstSFI CTRYAG 1 cut(s) 974
BstUI CGCG 2 cut(s) 610, 718
BstV1I GCAGC 1 cut(s) 562
BstV2I GAAGAC 2 cut(s) 206, 1251
BstXI CCANNNNNNTGG 1 cut(s) 860
BstZ17I GTATAC 1 cut(s) 929
BsuI GTATCC 1 cut(s) 593
BsuRI GGCC 4 cut(s) 518, 728, 826, 1108
BtgZI GCGATG 1 cut(s) 1248
BtsI GCAGTG 1 cut(s) 842
BtsIMutI CAGTG 3 cut(s) 177, 842, 1130
BveI ACCTGC 1 cut(s) 1030
Cac8I GCNNGC 6 cut(s) 54, 397, 477, 610, 1037, 1128
CciI TCATGA 1 cut(s) 1215
CfoI GCGC 3 cut(s) 550, 610, 612
Cfr10I RCCGGY 3 cut(s) 527, 614, 1126
Cfr13I GGNCC 1 cut(s) 516
CseI GACGC 1 cut(s) 403
Csp6I GTAC 1 cut(s) 290
CviAII CATG 6 cut(s) 266, 370, 809, 883, 1159, 1216
CviQI GTAC 1 cut(s) 290
DdeI CTNAG 5 cut(s) 208, 221, 677, 1017, 1246
DpnI GATC 5 cut(s) 32, 105, 781, 950, 1123
DpnII GATC 5 cut(s) 30, 103, 779, 948, 1121
EaeI YGGCCR 1 cut(s) 824
Eam1104I CTCTTC 1 cut(s) 930
EarI CTCTTC 1 cut(s) 930
EciI GGCGGA 1 cut(s) 556
Ecl136II GAGCTC 4 cut(s) 382, 676, 999, 1243
Eco130I CCWWGG 2 cut(s) 409, 827
Eco147I AGGCCT 2 cut(s) 728, 1108
Eco24I GRGCYC 4 cut(s) 384, 678, 1001, 1245
Eco53kI GAGCTC 4 cut(s) 382, 676, 999, 1243
Eco57I CTGAAG 2 cut(s) 218, 1039
EcoICRI GAGCTC 4 cut(s) 382, 676, 999, 1243
EcoRI GAATTC 1 cut(s) 149
EcoRII CCWGG 1 cut(s) 349
EcoT14I CCWWGG 2 cut(s) 409, 827
EcoT22I ATGCAT 1 cut(s) 961
EcoT38I GRGCYC 4 cut(s) 384, 678, 1001, 1245
ErhI CCWWGG 2 cut(s) 409, 827
FaeI CATG 6 cut(s) 269, 373, 812, 886, 1162, 1219
FalI AAGNNNNNCTT 2 cut(s) 191, 223
FatI CATG 6 cut(s) 265, 369, 808, 882, 1158, 1215
FbaI TGATCA 1 cut(s) 103
FblI GTMKAC 1 cut(s) 928
Fnu4HI GCNGC 4 cut(s) 519, 551, 554, 824
FriOI GRGCYC 4 cut(s) 384, 678, 1001, 1245
Fsp4HI GCNGC 4 cut(s) 519, 551, 554, 824
FspBI CTAG 2 cut(s) 239, 1148
GlaI GCGC 3 cut(s) 549, 609, 611
GluI GCNGC 4 cut(s) 519, 551, 554, 824
GsuI CTGGAG 1 cut(s) 372
HaeIII GGCC 4 cut(s) 518, 728, 826, 1108
HapII CCGG 6 cut(s) 119, 243, 528, 615, 909, 1127
HgaI GACGC 1 cut(s) 403
HhaI GCGC 3 cut(s) 550, 610, 612
Hin1I GRCGYC 1 cut(s) 414
Hin1II CATG 6 cut(s) 269, 373, 812, 886, 1162, 1219
Hin6I GCGC 3 cut(s) 548, 608, 610
HinP1I GCGC 3 cut(s) 548, 608, 610
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HinfI GANTC 1 cut(s) 249
HpaII CCGG 6 cut(s) 119, 243, 528, 615, 909, 1127
HphI GGTGA 2 cut(s) 613, 995
Hpy166II GTNNAC 5 cut(s) 290, 313, 817, 929, 1153
Hpy188I TCNGA 2 cut(s) 328, 680
Hpy188III TCNNGA 4 cut(s) 101, 1006, 1141, 1216
Hpy8I GTNNAC 5 cut(s) 290, 313, 817, 929, 1153
Hpy99I CGWCG 1 cut(s) 389
HpyAV CCTTC 1 cut(s) 723
HpyCH4III ACNGT 1 cut(s) 926
HpyCH4IV ACGT 8 cut(s) 426, 500, 931, 963, 1010, 1112, 1136, 1286
HpyCH4V TGCA 3 cut(s) 276, 318, 959
HpyF10VI GCNNNNNNNGC 4 cut(s) 379, 524, 698, 887
HpyF3I CTNAG 5 cut(s) 208, 221, 677, 1017, 1246
HpySE526I ACGT 8 cut(s) 426, 500, 931, 963, 1010, 1112, 1136, 1286
Hsp92I GRCGYC 1 cut(s) 414
Hsp92II CATG 6 cut(s) 269, 373, 812, 886, 1162, 1219
HspAI GCGC 3 cut(s) 548, 608, 610
KroI GCCGGC 1 cut(s) 1126
KroNI GCCGGC 1 cut(s) 1128
Ksp22I TGATCA 1 cut(s) 103
Kzo9I GATC 5 cut(s) 30, 103, 779, 948, 1121
LmnI GCTCC 3 cut(s) 673, 895, 1004
Lsp1109I GCAGC 1 cut(s) 562
LweI GCATC 3 cut(s) 595, 627, 710
MaeI CTAG 2 cut(s) 239, 1148
MaeII ACGT 8 cut(s) 426, 500, 931, 963, 1010, 1112, 1136, 1286
MaeIII GTNAC 3 cut(s) 634, 894, 1042
MalI GATC 5 cut(s) 32, 105, 781, 950, 1123
MboI GATC 5 cut(s) 30, 103, 779, 948, 1121
MboII GAAGA 4 cut(s) 68, 211, 947, 1251
MhlI GDGCHC 4 cut(s) 384, 678, 1001, 1245
MluCI AATT 3 cut(s) 149, 589, 1182
MmeI TCCRAC 2 cut(s) 86, 213
Mph1103I ATGCAT 1 cut(s) 961
MroNI GCCGGC 1 cut(s) 1126
MseI TTAA 3 cut(s) 593, 663, 746
MspCI CTTAAG 1 cut(s) 745
MspI CCGG 6 cut(s) 119, 243, 528, 615, 909, 1127
MspR9I CCNGG 3 cut(s) 119, 351, 910
MvaI CCWGG 1 cut(s) 351
MvnI CGCG 2 cut(s) 610, 718
MwoI GCNNNNNNNGC 4 cut(s) 379, 524, 698, 887
NaeI GCCGGC 1 cut(s) 1128
NciI CCSGG 2 cut(s) 119, 910
NdeII GATC 5 cut(s) 30, 103, 779, 948, 1121
NgoMIV GCCGGC 1 cut(s) 1126
NlaIII CATG 6 cut(s) 269, 373, 812, 886, 1162, 1219
NlaIV GGNNCC 2 cut(s) 866, 915
NsiI ATGCAT 1 cut(s) 961
NspI RCATGY 1 cut(s) 269
NspV TTCGAA 1 cut(s) 834
PagI TCATGA 1 cut(s) 1215
PauI GCGCGC 1 cut(s) 608
PceI AGGCCT 2 cut(s) 728, 1108
PciI ACATGT 1 cut(s) 265
PdiI GCCGGC 1 cut(s) 1128
PfeI GAWTC 1 cut(s) 249
PflMI CCANNNNNTGG 2 cut(s) 268, 350
PkrI GCNGC 4 cut(s) 520, 552, 555, 825
Ppu21I YACGTR 3 cut(s) 932, 964, 1113
PscI ACATGT 1 cut(s) 265
Psp124BI GAGCTC 4 cut(s) 384, 678, 1001, 1245
Psp6I CCWGG 1 cut(s) 349
PspGI CCWGG 1 cut(s) 349
PspN4I GGNNCC 2 cut(s) 866, 915
PspPI GGNCC 1 cut(s) 516
PteI GCGCGC 1 cut(s) 608
RsaI GTAC 1 cut(s) 291
RsaNI GTAC 1 cut(s) 290
SacI GAGCTC 4 cut(s) 384, 678, 1001, 1245
SaqAI TTAA 3 cut(s) 593, 663, 746
SatI GCNGC 4 cut(s) 519, 551, 554, 824
Sau3AI GATC 5 cut(s) 30, 103, 779, 948, 1121
Sau96I GGNCC 1 cut(s) 516
ScrFI CCNGG 3 cut(s) 119, 351, 910
SduI GDGCHC 4 cut(s) 384, 678, 1001, 1245
SfaNI GCATC 3 cut(s) 595, 627, 710
SfcI CTRYAG 1 cut(s) 974
SfuI TTCGAA 1 cut(s) 834
SgrAI CRCCGGYG 1 cut(s) 527
SmlI CTYRAG 2 cut(s) 306, 745
SmoI CTYRAG 2 cut(s) 306, 745
Sse9I AATT 3 cut(s) 149, 589, 1182
SseBI AGGCCT 2 cut(s) 728, 1108
SsiI CCGC 7 cut(s) 519, 525, 554, 567, 597, 823, 1268
SspMI CTAG 2 cut(s) 239, 1148
SstI GAGCTC 4 cut(s) 384, 678, 1001, 1245
StuI AGGCCT 2 cut(s) 728, 1108
StyD4I CCNGG 3 cut(s) 117, 349, 908
StyI CCWWGG 2 cut(s) 409, 827
TaaI ACNGT 1 cut(s) 926
TaiI ACGT 8 cut(s) 429, 503, 934, 966, 1013, 1115, 1139, 1289
TaqI TCGA 4 cut(s) 384, 834, 951, 1262
TasI AATT 3 cut(s) 149, 589, 1182
TauI GCSGC 3 cut(s) 521, 556, 826
TfiI GAWTC 1 cut(s) 249
Tru1I TTAA 3 cut(s) 593, 663, 746
Tru9I TTAA 3 cut(s) 593, 663, 746
TscAI CASTG 3 cut(s) 184, 849, 1137
TseI GCWGC 1 cut(s) 550
TspDTI ATGAA 6 cut(s) 26, 162, 550, 730, 797, 1147
TspGWI ACGGA 1 cut(s) 882
TspRI CASTG 3 cut(s) 184, 849, 1137
Van91I CCANNNNNTGG 2 cut(s) 268, 350
Vha464I CTTAAG 1 cut(s) 745
XapI RAATTY 2 cut(s) 149, 589
XceI RCATGY 1 cut(s) 269
XmiI GTMKAC 1 cut(s) 928
XspI CTAG 2 cut(s) 239, 1148
Zsp2I ATGCAT 1 cut(s) 961
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.