Prupe.1G236800_v2.0.a1

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000622
Physical Location & Seq
Reverse (-)
25095222 .. 25096822
1601 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G236800.1

Sequence Viewer

Length: 1317 bp
ATGAAGGTTGAAGTTGAAGTGATCTCCAAGGAGATAATCAAACCATCTTCTCCAACCCCTGACCATCTTTGCCACTACCAATTCTCCTTTCTTGATCAAATAGCTCCCCAAGTCTACAACCCTTTGCTCCTCTTCTATGAGCACAAAGCCAAGACACAATTCAACATCACTGAAATATCCAATCACCTCAAGAACTCATTATCCGAGGTCTTAACCCTTTTCTACCCTCTAGCCGGACGCATCAAGCACAACCAGTTCATGCATTGCAACGATGAGGGCATCCCTTTCTTTGAAGCTCGAGTACTGGATTGCACACTCTCTGATGTTCTTAATAACCCTATCCCTGGCGAGCTCAACAAGTTCATGCCCTTCGAACTTGATGATATTACCAATTCATTTCCCTTAGGGGTCCAGCTCAACATATTTCAATGTGGAGGCTTTGCAATTGGTCAATGCATTTCTCATAAGATTGCTGATGGGTTGTCGTATTTCATGTTCAGCAAAATTTGGGCAGCCATTGCTCGTGGAGACAAAGCCAACATAAACCCTCCGGAGTTTATTTCATCCACCCTCTTCCCACCAAAGGAGTTTAATATTGCATATGATGGCGGCGTTGGAATCACAAAGGATAGGGTAACAAAGCGGTTCGTGTTTGATTCCTCTCAAATAGAGAACCTCCGAGCAAGATATGGACACAATATGCACTTGCAGAAACGCCTAACACGAGTTGAAACCTTATCCACTTTTGTATGGAGTCGGTTCGTGGCGGCTACAACGGATTGTACCATGAACAAGTTGTATAGGGTGATCCATGCTGTGAACTTGCGTTCGAGGTTTGATCCACCATTGCCTCAAAGTTTTTTTGGGAATCTTTTTTGTATCTCCATGACAGCTCCACTACAAATTCCAAGTAGTGGTGGCGATGAAGAATGTGGTCACGACGTGGTGATCCAGGCGGTACGAGAAGCAGTAGGCAAGATAGACAATGAGTACGTGAAGAAACTACAAAAGCAAGATGATGAGCACTTGGGTGTGATGAAGAAACAAGCTGATAGTTTTAAGAGAGGAGAGATGGGTACGTTACTTAGCTTTAGTAGCTATTGTAGATTTCCTCTTTATGACAATGATTTTGGTTGGGGAAGGCCTACGTGGGTCGGCTCACCCTCACTCACCTACAAGAACGTTGTGCTTTTCATGGACACAAAAGAGGGTGGTGGAATAGAGGCATATGTTAGTTTGGAGGAGGAAGTCATGGCTAAGTTTGAATGTGATAGCGAGTTACTCTCCTATGTTGCTCCAACGGTCGGGTGCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

439

Amino Acids

49.51

Weight (kDa)

5.87

Isoelectric Point (pI)

51.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24710 FvH4_4g24720 FvH4_4g24770 FvH4_4g24771 FvH4_4g24772 FvH4_5g39380
malus_domestica MD10G1199500.v1.1 MD13G1109100.v1.1 MD13G1109200.v1.1 MD13G1114400.v1.1 MD13G1114800.v1.1 MD16G1108800.v1.1
prunus_persica Prupe.1G235900_v2.0.a1 Prupe.1G236600_v2.0.a1 Prupe.1G236700_v2.0.a1 Prupe.1G236800_v2.0.a1 Prupe.4G142000_v2.0.a1
pyrus_communis pycom10g17180 pycom13g09460 pycom13g09930 pycom13g09940
rosa_chinensis RchiOBHm_Chr3g0490281 RchiOBHm_Chr3g0490291 RchiOBHm_Chr3g0490301 RchiOBHm_Chr4g0417441 RchiOBHm_Chr4g0417461 RchiOBHm_Chr4g0431811 RchiOBHm_Chr4g0431821 RchiOBHm_Chr4g0431851
rosa_laevigata RLG00000006882 RLG00000006886 RLG00000006887 RLG00000007905 RLG00000007911 RLG00000007913 RLG00000029538
rosa_multiflora Rmu_sc0001459.1_g000008 Rmu_sc0001459.1_g000011 Rmu_sc0002489.1_g000020 Rmu_sc0002489.1_g000024 Rmu_sc0002489.1_g000027 Rmu_sc0002489.1_g000028 Rmu_sc0002489.1_g000029 Rmu_sc0004142.1_g000001 Rmu_sc0004142.1_g000024 Rmu_sc0011237.1_g000018 Rmu_sc0031439.1_g000001 Rmu_sc0031677.1_g000001 Rmu_ssc0000361.1_g000005
rosa_roxburghii Rroxscaffold_3G00262760 Rroxscaffold_4G00317310 Rroxscaffold_5G00361150 Rroxscaffold_5G00373190 Rroxscaffold_5G00373210 Rroxscaffold_5G00373220 Rroxscaffold_5G00373240
rosa_rugosa Rorug01G0111100 Rorug04G0252400 Rorug04G0252400 Rorug04G0252400 Rorug04G0252600
rosa_samantha Rh1BG102300 Rh3AG292200 Rh3AG292300 Rh4AG309100 Rh4AG309300 Rh4AG309500 Rh6BG200900
rosa_wichuraiana Rw0G002990 Rw0G003000 Rw0G019170 Rw3G025890 Rw3G025900 Rw4G017830 Rw4G026810 Rw4G026870 Rw4G026880 Rw4G026920 Rw6G017190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 914
AccI GTMKAC 1 cut(s) 114
AccIII TCCGGA 1 cut(s) 550
AciI CCGC 4 cut(s) 609, 643, 767, 956
AclI AACGTT 1 cut(s) 1182
AclWI GGATC 3 cut(s) 802, 833, 943
AcsI RAATTY 2 cut(s) 504, 903
AdeI CACNNNGTG 1 cut(s) 943
AfaI GTAC 5 cut(s) 303, 784, 960, 992, 1078
AfiI CCNNNNNNNGG 5 cut(s) 233, 344, 583, 914, 1304
AgsI TTSAA 7 cut(s) 11, 17, 163, 293, 428, 731, 1265
AjiI CACGTC 1 cut(s) 943
AjnI CCWGG 2 cut(s) 343, 951
AjuI GAANNNNNNNTTGG 4 cut(s) 72, 104, 143, 175
AleI CACNNNNGTG 1 cut(s) 1029
AloI GAACNNNNNNTCC 2 cut(s) 185, 217
AluBI AGCT 8 cut(s) 104, 296, 352, 415, 893, 1049, 1089, 1098
AluI AGCT 8 cut(s) 104, 296, 352, 415, 893, 1049, 1089, 1098
Alw21I GWGCWC 3 cut(s) 144, 354, 1026
Alw26I GTCTC 1 cut(s) 522
AlwI GGATC 3 cut(s) 802, 833, 943
Ama87I CYCGRG 1 cut(s) 297
Aor13HI TCCGGA 1 cut(s) 550
AoxI GGCC 1 cut(s) 1142
ApeKI GCWGC 2 cut(s) 512, 1311
ApoI RAATTY 2 cut(s) 504, 903
ArsI GACNNNNNNTTYG 2 cut(s) 1112, 1144
AspS9I GGNCC 1 cut(s) 409
AsuHPI GGTGA 5 cut(s) 176, 817, 958, 1152, 1162
AsuII TTCGAA 1 cut(s) 372
AvaI CYCGRG 1 cut(s) 297
AvaII GGWCC 1 cut(s) 409
AxyI CCTNAGG 1 cut(s) 403
BanII GRGCYC 1 cut(s) 354
BarI GAAGNNNNNNTAC 2 cut(s) 285, 317
BauI CACGAG 2 cut(s) 522, 723
Bbv12I GWGCWC 3 cut(s) 144, 354, 1026
BbvI GCAGC 2 cut(s) 524, 1298
BccI CCATC 5 cut(s) 52, 72, 470, 599, 1066
BciT130I CCWGG 2 cut(s) 345, 953
BclI TGATCA 1 cut(s) 94
BcoDI GTCTC 1 cut(s) 522
BfaI CTAG 1 cut(s) 230
BisI GCNGC 4 cut(s) 513, 610, 768, 1312
BlsI GCNGC 4 cut(s) 514, 611, 769, 1313
BmcAI AGTACT 1 cut(s) 303
Bme1390I CCNGG 2 cut(s) 345, 953
Bme18I GGWCC 1 cut(s) 409
BmeT110I CYCGRG 1 cut(s) 297
BmgBI CACGTC 1 cut(s) 943
BmgT120I GGNCC 1 cut(s) 409
BmiI GGNNCC 1 cut(s) 410
BmrFI CCNGG 2 cut(s) 345, 953
BmsI GCATC 2 cut(s) 249, 288
BplI GAGNNNNNCTC 2 cut(s) 1268, 1300
Bpu14I TTCGAA 1 cut(s) 372
BpuEI CTTGAG 1 cut(s) 173
BsaAI YACGTR 2 cut(s) 994, 1149
BsaJI CCNNGG 3 cut(s) 27, 204, 343
BsaWI WCCGGW 1 cut(s) 550
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bsc4I CCNNNNNNNGG 5 cut(s) 233, 344, 583, 914, 1304
Bse1I ACTGG 2 cut(s) 253, 309
Bse21I CCTNAGG 1 cut(s) 403
Bse3DI GCAATG 3 cut(s) 262, 516, 845
BseAI TCCGGA 1 cut(s) 550
BseBI CCWGG 2 cut(s) 345, 953
BseDI CCNNGG 3 cut(s) 27, 204, 343
BseGI GGATG 2 cut(s) 279, 563
BseLI CCNNNNNNNGG 5 cut(s) 233, 344, 583, 914, 1304
BseMI GCAATG 3 cut(s) 262, 516, 845
BseNI ACTGG 2 cut(s) 253, 309
BseRI GAGGAG 3 cut(s) 119, 1080, 1256
BseXI GCAGC 2 cut(s) 524, 1298
Bsh1285I CGRYCG 1 cut(s) 1305
BshFI GGCC 1 cut(s) 1144
BsiEI CGRYCG 1 cut(s) 1305
BsiHKAI GWGCWC 3 cut(s) 144, 354, 1026
BsiHKCI CYCGRG 1 cut(s) 297
BsiSI CCGG 2 cut(s) 234, 551
BslI CCNNNNNNNGG 5 cut(s) 233, 344, 583, 914, 1304
BsmAI GTCTC 1 cut(s) 522
BsnI GGCC 1 cut(s) 1144
BsoBI CYCGRG 1 cut(s) 297
Bsp119I TTCGAA 1 cut(s) 372
Bsp1286I GDGCHC 3 cut(s) 144, 354, 1026
Bsp13I TCCGGA 1 cut(s) 550
Bsp143I GATC 5 cut(s) 21, 94, 807, 838, 948
BspACI CCGC 4 cut(s) 609, 643, 767, 956
BspANI GGCC 1 cut(s) 1144
BspEI TCCGGA 1 cut(s) 550
BspLI GGNNCC 1 cut(s) 410
BspPI GGATC 3 cut(s) 802, 833, 943
BspT104I TTCGAA 1 cut(s) 372
BsrDI GCAATG 3 cut(s) 262, 516, 845
BsrI ACTGG 2 cut(s) 253, 309
BssECI CCNNGG 3 cut(s) 27, 204, 343
BssMI GATC 5 cut(s) 21, 94, 807, 838, 948
BssSI CACGAG 2 cut(s) 522, 723
BssT1I CCWWGG 1 cut(s) 27
Bst2BI CACGAG 2 cut(s) 522, 723
Bst2UI CCWGG 2 cut(s) 345, 953
Bst4CI ACNGT 1 cut(s) 1303
Bst6I CTCTTC 2 cut(s) 137, 578
BstAPI GCANNNNNTGC 1 cut(s) 518
BstBAI YACGTR 2 cut(s) 994, 1149
BstBI TTCGAA 1 cut(s) 372
BstC8I GCNNGC 1 cut(s) 350
BstDEI CTNAG 3 cut(s) 403, 1085, 1257
BstF5I GGATG 2 cut(s) 279, 563
BstKTI GATC 5 cut(s) 24, 97, 810, 841, 951
BstMAI GTCTC 1 cut(s) 522
BstMBI GATC 5 cut(s) 21, 94, 807, 838, 948
BstMCI CGRYCG 1 cut(s) 1305
BstMWI GCNNNNNNNGC 2 cut(s) 518, 1095
BstNI CCWGG 2 cut(s) 345, 953
BstSCI CCNGG 2 cut(s) 343, 951
BstV1I GCAGC 2 cut(s) 524, 1298
Bsu36I CCTNAGG 1 cut(s) 403
BsuRI GGCC 1 cut(s) 1144
BtgZI GCGATG 1 cut(s) 936
BtrI CACGTC 1 cut(s) 943
BtsCI GGATG 2 cut(s) 279, 563
BtsIMutI CAGTG 1 cut(s) 168
Cac8I GCNNGC 1 cut(s) 350
Cfr13I GGNCC 1 cut(s) 409
CseI GACGC 1 cut(s) 246
Csp6I GTAC 5 cut(s) 302, 783, 959, 991, 1077
CviAII CATG 8 cut(s) 259, 364, 493, 787, 812, 886, 1195, 1252
CviQI GTAC 5 cut(s) 302, 783, 959, 991, 1077
DdeI CTNAG 3 cut(s) 403, 1085, 1257
DpnI GATC 5 cut(s) 23, 96, 809, 840, 950
DpnII GATC 5 cut(s) 21, 94, 807, 838, 948
DraIII CACNNNGTG 1 cut(s) 943
Eam1104I CTCTTC 2 cut(s) 137, 578
EarI CTCTTC 2 cut(s) 137, 578
Ecl136II GAGCTC 1 cut(s) 352
Eco130I CCWWGG 1 cut(s) 27
Eco147I AGGCCT 1 cut(s) 1144
Eco24I GRGCYC 1 cut(s) 354
Eco47I GGWCC 1 cut(s) 409
Eco53kI GAGCTC 1 cut(s) 352
Eco81I CCTNAGG 1 cut(s) 403
Eco88I CYCGRG 1 cut(s) 297
EcoICRI GAGCTC 1 cut(s) 352
EcoRII CCWGG 2 cut(s) 343, 951
EcoT14I CCWWGG 1 cut(s) 27
EcoT22I ATGCAT 2 cut(s) 264, 458
EcoT38I GRGCYC 1 cut(s) 354
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 8 cut(s) 262, 367, 496, 790, 815, 889, 1198, 1255
FatI CATG 8 cut(s) 258, 363, 492, 786, 811, 885, 1194, 1251
FauNDI CATATG 2 cut(s) 601, 1228
FbaI TGATCA 1 cut(s) 94
FblI GTMKAC 1 cut(s) 114
Fnu4HI GCNGC 4 cut(s) 513, 610, 768, 1312
FokI GGATG 2 cut(s) 266, 550
FriOI GRGCYC 1 cut(s) 354
Fsp4HI GCNGC 4 cut(s) 513, 610, 768, 1312
FspBI CTAG 1 cut(s) 230
GluI GCNGC 4 cut(s) 513, 610, 768, 1312
HaeIII GGCC 1 cut(s) 1144
HapII CCGG 2 cut(s) 234, 551
HgaI GACGC 1 cut(s) 246
Hin1II CATG 8 cut(s) 262, 367, 496, 790, 815, 889, 1198, 1255
HinfI GANTC 4 cut(s) 618, 656, 754, 868
HpaII CCGG 2 cut(s) 234, 551
HphI GGTGA 5 cut(s) 176, 817, 958, 1152, 1162
Hpy166II GTNNAC 2 cut(s) 115, 820
Hpy188I TCNGA 3 cut(s) 205, 322, 680
Hpy188III TCNNGA 4 cut(s) 92, 190, 551, 938
Hpy8I GTNNAC 2 cut(s) 115, 820
Hpy99I CGWCG 1 cut(s) 944
HpyAV CCTTC 2 cut(s) 379, 1134
HpyCH4III ACNGT 1 cut(s) 1303
HpyCH4IV ACGT 5 cut(s) 942, 993, 1079, 1148, 1182
HpyCH4V TGCA 8 cut(s) 262, 267, 312, 443, 456, 599, 703, 709
HpyF10VI GCNNNNNNNGC 2 cut(s) 518, 1095
HpyF3I CTNAG 3 cut(s) 403, 1085, 1257
HpySE526I ACGT 5 cut(s) 942, 993, 1079, 1148, 1182
Hsp92II CATG 8 cut(s) 262, 367, 496, 790, 815, 889, 1198, 1255
Kpn2I TCCGGA 1 cut(s) 550
Ksp22I TGATCA 1 cut(s) 94
Kzo9I GATC 5 cut(s) 21, 94, 807, 838, 948
LmnI GCTCC 4 cut(s) 109, 132, 898, 1300
Lsp1109I GCAGC 2 cut(s) 524, 1298
LweI GCATC 2 cut(s) 249, 288
MaeI CTAG 1 cut(s) 230
MaeII ACGT 5 cut(s) 942, 993, 1079, 1148, 1182
MaeIII GTNAC 4 cut(s) 634, 935, 1080, 1278
MalI GATC 5 cut(s) 23, 96, 809, 840, 950
MboI GATC 5 cut(s) 21, 94, 807, 838, 948
MboII GAAGA 6 cut(s) 39, 124, 565, 938, 1009, 1051
MfeI CAATTG 1 cut(s) 444
MhlI GDGCHC 3 cut(s) 144, 354, 1026
MluCI AATT 6 cut(s) 80, 158, 391, 444, 504, 903
MlyI GAGTC 1 cut(s) 763
MmeI TCCRAC 2 cut(s) 77, 595
Mph1103I ATGCAT 2 cut(s) 264, 458
MroI TCCGGA 1 cut(s) 550
MseI TTAA 4 cut(s) 212, 330, 591, 1059
MslI CAYNNNNRTG 1 cut(s) 1029
MspI CCGG 2 cut(s) 234, 551
MspR9I CCNGG 2 cut(s) 345, 953
MunI CAATTG 1 cut(s) 444
MvaI CCWGG 2 cut(s) 345, 953
MwoI GCNNNNNNNGC 2 cut(s) 518, 1095
NdeI CATATG 2 cut(s) 601, 1228
NdeII GATC 5 cut(s) 21, 94, 807, 838, 948
NlaIII CATG 8 cut(s) 262, 367, 496, 790, 815, 889, 1198, 1255
NlaIV GGNNCC 1 cut(s) 410
NmuCI GTSAC 1 cut(s) 935
NsiI ATGCAT 2 cut(s) 264, 458
NspV TTCGAA 1 cut(s) 372
OliI CACNNNNGTG 1 cut(s) 1029
PaeR7I CTCGAG 1 cut(s) 297
PceI AGGCCT 1 cut(s) 1144
PcsI WCGNNNNNNNCGW 1 cut(s) 721
PfeI GAWTC 3 cut(s) 618, 656, 868
PflMI CCANNNNNTGG 1 cut(s) 914
PkrI GCNGC 4 cut(s) 514, 611, 769, 1313
PleI GAGTC 1 cut(s) 762
PpsI GAGTC 1 cut(s) 762
Ppu21I YACGTR 2 cut(s) 994, 1149
Psp124BI GAGCTC 1 cut(s) 354
Psp1406I AACGTT 1 cut(s) 1182
Psp6I CCWGG 2 cut(s) 343, 951
PspGI CCWGG 2 cut(s) 343, 951
PspN4I GGNNCC 1 cut(s) 410
PspPI GGNCC 1 cut(s) 409
PspXI VCTCGAGB 1 cut(s) 297
PsrI GAACNNNNNNTAC 2 cut(s) 479, 511
RsaI GTAC 5 cut(s) 303, 784, 960, 992, 1078
RsaNI GTAC 5 cut(s) 302, 783, 959, 991, 1077
RseI CAYNNNNRTG 1 cut(s) 1029
SacI GAGCTC 1 cut(s) 354
SaqAI TTAA 4 cut(s) 212, 330, 591, 1059
SatI GCNGC 4 cut(s) 513, 610, 768, 1312
Sau3AI GATC 5 cut(s) 21, 94, 807, 838, 948
Sau96I GGNCC 1 cut(s) 409
ScaI AGTACT 1 cut(s) 303
SchI GAGTC 1 cut(s) 763
ScrFI CCNGG 2 cut(s) 345, 953
SduI GDGCHC 3 cut(s) 144, 354, 1026
SfaNI GCATC 2 cut(s) 249, 288
Sfr274I CTCGAG 1 cut(s) 297
SfuI TTCGAA 1 cut(s) 372
SinI GGWCC 1 cut(s) 409
SlaI CTCGAG 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 1029
SmlI CTYRAG 2 cut(s) 188, 297
SmoI CTYRAG 2 cut(s) 188, 297
Sse9I AATT 6 cut(s) 80, 158, 391, 444, 504, 903
SseBI AGGCCT 1 cut(s) 1144
SsiI CCGC 4 cut(s) 609, 643, 767, 956
SspI AATATT 1 cut(s) 595
SspMI CTAG 1 cut(s) 230
SstI GAGCTC 1 cut(s) 354
StuI AGGCCT 1 cut(s) 1144
StyD4I CCNGG 2 cut(s) 343, 951
StyI CCWWGG 1 cut(s) 27
TaaI ACNGT 1 cut(s) 1303
TaiI ACGT 5 cut(s) 945, 996, 1082, 1151, 1185
TaqI TCGA 3 cut(s) 298, 372, 830
TasI AATT 6 cut(s) 80, 158, 391, 444, 504, 903
TatI WGTACW 1 cut(s) 301
TauI GCSGC 2 cut(s) 612, 770
TfiI GAWTC 3 cut(s) 618, 656, 868
Tru1I TTAA 4 cut(s) 212, 330, 591, 1059
Tru9I TTAA 4 cut(s) 212, 330, 591, 1059
TscAI CASTG 1 cut(s) 175
TseFI GTSAC 1 cut(s) 935
TseI GCWGC 2 cut(s) 512, 1311
Tsp45I GTSAC 1 cut(s) 935
TspGWI ACGGA 1 cut(s) 791
TspRI CASTG 1 cut(s) 175
Van91I CCANNNNNTGG 1 cut(s) 914
VpaK11BI GGWCC 1 cut(s) 409
XapI RAATTY 2 cut(s) 504, 903
XhoI CTCGAG 1 cut(s) 297
XmiI GTMKAC 1 cut(s) 114
XspI CTAG 1 cut(s) 230
ZrmI AGTACT 1 cut(s) 303
Zsp2I ATGCAT 2 cut(s) 264, 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.