Rw3G025890

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000622
Physical Location & Seq
Reverse (-)
34969467 .. 34970804
1338 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G025890.1

Sequence Viewer

Length: 1191 bp
ATGAAGGTTGAAGTAGAAGTAATCTCCAAGGAGATTATCACCCCATCTTCTCCAACCCCTGCTCATCTTCGCCATTACCAGCTCTCCTCCTTTGATCAATTAGCTCCCCCAGTCTATAACCCTTTGGTCCTCTTCTACGAATTCAATGATAAGACAGCCCCCAACATTACTGAAATATCCAGCCACCTCAAAAAGTCCCTAGCCGAAGTCTTAACCGTTTTCTACCCATTAGTCGGACGAATCAAACACGATGACTGGCATAATCCGATCCTTGATCAACATAGCAAGTTCATGCCATTTGAACTTGATGATCATATTGGTAAGGAGTTGGTCCTAGGGGTCCAGCTCAGCATCTTTGAATGTGGAGGATTTGCAATTGGTCAATGCATCTCTCACAGGCTTGCCGATGCGTCATCTTATTTCATGTTCAGCAAAACTTGGGCGGCCATAGCCCATGGCGAATCCAACATAGAGCATCCAGACTTTCTGTCAGCAACACGTTTTCCACTGAAGGATGTTATGGCATATGATCCAAGCACTGAAATCACCAGGAATAAAGTGACAAAGAGGTTTTTATTCAGTGCCTCTATGATAGAGGCTCTCAGAGCAAAGTATGGGGAAAGCGCTGTAGGCTTAGAAGAAAACCGGAAACGCCCATCACGTAAGTTGTATATTGCGGTCCATGCTGTGAACCTACGCCCAAGGTTTGATCCACCATTGCCACAACATTCTTTTGGAAACATTTACCGTGCTGCCACCACAGGTCCCTTACTAAGTACTGGGGATGCATGCAGTGCATATGGTCGGGCTATGAGGGAATTACGAGAAGATATAAACAAGATCGACAATGACTGCGTCAAAGGACTACAGCAGGGTGCTGAGCAGTCGGGGCTCCCCTTCAATGAGAATACTGGTAGACTAACTGAACTAGAGCTGGCTAAGTTAGTCTTCAGTAGCTATTGCAGGTTTCCAGTGTATGATAATGATTTCGGTTGGGGAAGGCCTGCGTGGGTGTTCTCAACACCATTGACCTTCAAGAACGTAGCAACTTTCATGGACACCAAAGAGGGTGATGGAATAGAGGTATACATCAGTTTGGAGGAGCAACTCATGGCTAAATTTGAAACTGATGCCGAGTTCTTAGCCCCTATGTGTCTCCAAGTGGGCGCTGAAGAGCCCTTTGGTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

396

Amino Acids

44.56

Weight (kDa)

5.6

Isoelectric Point (pI)

50.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 4 - 85 1.6e-17 Transferase family
Transferase PF02458 93 - 380 1.2e-36 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24710 FvH4_4g24720 FvH4_4g24770 FvH4_4g24771 FvH4_4g24772 FvH4_5g39380
malus_domestica MD10G1199500.v1.1 MD13G1109100.v1.1 MD13G1109200.v1.1 MD13G1114400.v1.1 MD13G1114800.v1.1 MD16G1108800.v1.1
prunus_persica Prupe.1G235900_v2.0.a1 Prupe.1G236600_v2.0.a1 Prupe.1G236700_v2.0.a1 Prupe.1G236800_v2.0.a1 Prupe.4G142000_v2.0.a1
pyrus_communis pycom10g17180 pycom13g09460 pycom13g09930 pycom13g09940
rosa_chinensis RchiOBHm_Chr3g0490281 RchiOBHm_Chr3g0490291 RchiOBHm_Chr3g0490301 RchiOBHm_Chr4g0417441 RchiOBHm_Chr4g0417461 RchiOBHm_Chr4g0431811 RchiOBHm_Chr4g0431821 RchiOBHm_Chr4g0431851
rosa_laevigata RLG00000006882 RLG00000006886 RLG00000006887 RLG00000007905 RLG00000007911 RLG00000007913 RLG00000029538
rosa_multiflora Rmu_sc0001459.1_g000008 Rmu_sc0001459.1_g000011 Rmu_sc0002489.1_g000020 Rmu_sc0002489.1_g000024 Rmu_sc0002489.1_g000027 Rmu_sc0002489.1_g000028 Rmu_sc0002489.1_g000029 Rmu_sc0004142.1_g000001 Rmu_sc0004142.1_g000024 Rmu_sc0011237.1_g000018 Rmu_sc0031439.1_g000001 Rmu_sc0031677.1_g000001 Rmu_ssc0000361.1_g000005
rosa_roxburghii Rroxscaffold_3G00262760 Rroxscaffold_4G00317310 Rroxscaffold_5G00361150 Rroxscaffold_5G00373190 Rroxscaffold_5G00373210 Rroxscaffold_5G00373220 Rroxscaffold_5G00373240
rosa_rugosa Rorug01G0111100 Rorug04G0252400 Rorug04G0252400 Rorug04G0252400 Rorug04G0252600
rosa_samantha Rh1BG102300 Rh3AG292200 Rh3AG292300 Rh4AG309100 Rh4AG309300 Rh4AG309500 Rh6BG200900
rosa_wichuraiana Rw0G002990 Rw0G003000 Rw0G019170 Rw3G025890 Rw3G025900 Rw4G017830 Rw4G026810 Rw4G026870 Rw4G026880 Rw4G026920 Rw6G017190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 954
AccI GTMKAC 2 cut(s) 916, 1086
AciI CCGC 2 cut(s) 443, 677
AclWI GGATC 3 cut(s) 262, 524, 704
AcoI YGGCCR 1 cut(s) 444
AcsI RAATTY 2 cut(s) 140, 1118
AcuI CTGAAG 3 cut(s) 530, 934, 1191
AfaI GTAC 1 cut(s) 778
AfeI AGCGCT 1 cut(s) 625
AfiI CCNNNNNNNGG 1 cut(s) 233
AflIII ACRYGT 1 cut(s) 497
AgsI TTSAA 7 cut(s) 11, 145, 302, 359, 901, 1036, 1124
AhdI GACNNNNNGTC 1 cut(s) 487
AjnI CCWGG 1 cut(s) 548
AjuI GAANNNNNNNTTGG 1 cut(s) 1164
AluBI AGCT 5 cut(s) 82, 104, 346, 934, 957
AluI AGCT 5 cut(s) 82, 104, 346, 934, 957
Alw26I GTCTC 1 cut(s) 1160
AlwI GGATC 3 cut(s) 262, 524, 704
Aor51HI AGCGCT 1 cut(s) 625
AoxI GGCC 2 cut(s) 444, 1001
ApeKI GCWGC 1 cut(s) 752
ApoI RAATTY 2 cut(s) 140, 1118
AspA2I CCTAGG 1 cut(s) 334
AspLEI GCGC 2 cut(s) 626, 1169
AspS9I GGNCC 5 cut(s) 127, 331, 340, 679, 764
AsuHPI GGTGA 3 cut(s) 31, 538, 1082
AvaII GGWCC 5 cut(s) 127, 331, 340, 679, 764
AvrII CCTAGG 1 cut(s) 334
BanII GRGCYC 2 cut(s) 894, 1179
BbsI GAAGAC 1 cut(s) 940
BbvI GCAGC 1 cut(s) 739
BccI CCATC 3 cut(s) 52, 664, 1067
BciT130I CCWGG 1 cut(s) 550
BclI TGATCA 3 cut(s) 94, 274, 310
BcoDI GTCTC 1 cut(s) 1160
BfaI CTAG 3 cut(s) 200, 335, 929
BfmI CTRYAG 2 cut(s) 627, 866
BfoI RGCGCY 2 cut(s) 627, 1170
BfuAI ACCTGC 1 cut(s) 954
BisI GCNGC 2 cut(s) 444, 753
BlnI CCTAGG 1 cut(s) 334
BlpI GCTNAGC 2 cut(s) 347, 879
BlsI GCNGC 2 cut(s) 445, 754
BmcAI AGTACT 1 cut(s) 778
Bme1390I CCNGG 1 cut(s) 550
Bme18I GGWCC 5 cut(s) 127, 331, 340, 679, 764
BmeRI GACNNNNNGTC 1 cut(s) 487
BmgT120I GGNCC 5 cut(s) 127, 331, 340, 679, 764
BmiI GGNNCC 3 cut(s) 341, 766, 893
BmrFI CCNGG 1 cut(s) 550
BmrI ACTGGG 2 cut(s) 104, 789
BmsI GCATC 6 cut(s) 360, 396, 397, 484, 775, 1120
BmuI ACTGGG 2 cut(s) 104, 789
BpiI GAAGAC 1 cut(s) 940
Bpu1102I GCTNAGC 2 cut(s) 347, 879
BsaAI YACGTR 1 cut(s) 662
BsaJI CCNNGG 4 cut(s) 27, 334, 454, 701
BsaWI WCCGGW 1 cut(s) 645
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bsc4I CCNNNNNNNGG 1 cut(s) 233
Bse1I ACTGG 5 cut(s) 110, 260, 784, 916, 971
Bse3DI GCAATG 1 cut(s) 716
BseBI CCWGG 1 cut(s) 550
BseDI CCNNGG 4 cut(s) 27, 334, 454, 701
BseGI GGATG 3 cut(s) 475, 520, 790
BseLI CCNNNNNNNGG 1 cut(s) 233
BseMI GCAATG 1 cut(s) 716
BseMII CTCAG 3 cut(s) 361, 616, 870
BseNI ACTGG 5 cut(s) 110, 260, 784, 916, 971
BseRI GAGGAG 2 cut(s) 76, 1115
BseXI GCAGC 1 cut(s) 739
BshFI GGCC 2 cut(s) 446, 1003
BsiSI CCGG 1 cut(s) 646
BslFI GGGAC 2 cut(s) 181, 750
BslI CCNNNNNNNGG 1 cut(s) 233
BsmAI GTCTC 1 cut(s) 1160
BsmFI GGGAC 2 cut(s) 181, 750
BsnI GGCC 2 cut(s) 446, 1003
Bsp1286I GDGCHC 2 cut(s) 894, 1179
Bsp143I GATC 7 cut(s) 94, 267, 274, 310, 529, 709, 840
Bsp1720I GCTNAGC 2 cut(s) 347, 879
Bsp19I CCATGG 1 cut(s) 454
BspACI CCGC 2 cut(s) 443, 677
BspANI GGCC 2 cut(s) 446, 1003
BspCNI CTCAG 3 cut(s) 360, 615, 871
BspLI GGNNCC 3 cut(s) 341, 766, 893
BspMI ACCTGC 1 cut(s) 954
BspPI GGATC 3 cut(s) 262, 524, 704
BspQI GCTCTTC 1 cut(s) 1167
BsrDI GCAATG 1 cut(s) 716
BsrI ACTGG 5 cut(s) 110, 260, 784, 916, 971
BssECI CCNNGG 4 cut(s) 27, 334, 454, 701
BssMI GATC 7 cut(s) 94, 267, 274, 310, 529, 709, 840
BssNAI GTATAC 1 cut(s) 1087
BssT1I CCWWGG 4 cut(s) 27, 334, 454, 701
Bst1107I GTATAC 1 cut(s) 1087
Bst2UI CCWGG 1 cut(s) 550
Bst4CI ACNGT 2 cut(s) 217, 749
Bst6I CTCTTC 2 cut(s) 137, 1167
BstAPI GCANNNNNTGC 1 cut(s) 794
BstBAI YACGTR 1 cut(s) 662
BstC8I GCNNGC 4 cut(s) 402, 790, 936, 1005
BstDEI CTNAG 7 cut(s) 347, 602, 634, 773, 879, 939, 1141
BstDSI CCRYGG 1 cut(s) 454
BstF5I GGATG 3 cut(s) 475, 520, 790
BstH2I RGCGCY 2 cut(s) 627, 1170
BstHHI GCGC 2 cut(s) 626, 1169
BstKTI GATC 7 cut(s) 97, 270, 277, 313, 532, 712, 843
BstMAI GTCTC 1 cut(s) 1160
BstMBI GATC 7 cut(s) 94, 267, 274, 310, 529, 709, 840
BstMWI GCNNNNNNNGC 6 cut(s) 449, 605, 630, 683, 794, 889
BstNI CCWGG 1 cut(s) 550
BstNSI RCATGY 1 cut(s) 792
BstSCI CCNGG 1 cut(s) 548
BstSFI CTRYAG 2 cut(s) 627, 866
BstV1I GCAGC 1 cut(s) 739
BstV2I GAAGAC 1 cut(s) 940
BstZ17I GTATAC 1 cut(s) 1087
BsuRI GGCC 2 cut(s) 446, 1003
BtgI CCRYGG 1 cut(s) 454
BtsCI GGATG 3 cut(s) 475, 520, 790
BtsI GCAGTG 1 cut(s) 799
BtsIMutI CAGTG 5 cut(s) 506, 537, 586, 799, 978
BveI ACCTGC 1 cut(s) 954
Cac8I GCNNGC 4 cut(s) 402, 790, 936, 1005
CfoI GCGC 2 cut(s) 626, 1169
Cfr13I GGNCC 5 cut(s) 127, 331, 340, 679, 764
CseI GACGC 2 cut(s) 399, 844
Csp6I GTAC 1 cut(s) 777
CviAII CATG 7 cut(s) 292, 424, 455, 683, 789, 1054, 1111
CviQI GTAC 1 cut(s) 777
DdeI CTNAG 7 cut(s) 347, 602, 634, 773, 879, 939, 1141
DpnI GATC 7 cut(s) 96, 269, 276, 312, 531, 711, 842
DpnII GATC 7 cut(s) 94, 267, 274, 310, 529, 709, 840
DriI GACNNNNNGTC 1 cut(s) 487
EaeI YGGCCR 1 cut(s) 444
Eam1104I CTCTTC 2 cut(s) 137, 1167
Eam1105I GACNNNNNGTC 1 cut(s) 487
EarI CTCTTC 2 cut(s) 137, 1167
Eco130I CCWWGG 4 cut(s) 27, 334, 454, 701
Eco147I AGGCCT 1 cut(s) 1003
Eco24I GRGCYC 2 cut(s) 894, 1179
Eco47I GGWCC 5 cut(s) 127, 331, 340, 679, 764
Eco47III AGCGCT 1 cut(s) 625
Eco57I CTGAAG 3 cut(s) 530, 934, 1191
EcoO109I RGGNCCY 1 cut(s) 764
EcoRI GAATTC 1 cut(s) 140
EcoRII CCWGG 1 cut(s) 548
EcoT14I CCWWGG 4 cut(s) 27, 334, 454, 701
EcoT22I ATGCAT 2 cut(s) 389, 790
EcoT38I GRGCYC 2 cut(s) 894, 1179
ErhI CCWWGG 4 cut(s) 27, 334, 454, 701
FaeI CATG 7 cut(s) 295, 427, 458, 686, 792, 1057, 1114
FalI AAGNNNNNCTT 2 cut(s) 932, 964
FaqI GGGAC 2 cut(s) 181, 750
FatI CATG 7 cut(s) 291, 423, 454, 682, 788, 1053, 1110
FauNDI CATATG 2 cut(s) 526, 799
FbaI TGATCA 3 cut(s) 94, 274, 310
FblI GTMKAC 2 cut(s) 916, 1086
Fnu4HI GCNGC 2 cut(s) 444, 753
FokI GGATG 3 cut(s) 462, 527, 797
FriOI GRGCYC 2 cut(s) 894, 1179
Fsp4HI GCNGC 2 cut(s) 444, 753
FspBI CTAG 3 cut(s) 200, 335, 929
GlaI GCGC 2 cut(s) 625, 1168
GluI GCNGC 2 cut(s) 444, 753
HaeII RGCGCY 2 cut(s) 627, 1170
HaeIII GGCC 2 cut(s) 446, 1003
HapII CCGG 1 cut(s) 646
HgaI GACGC 2 cut(s) 399, 844
HhaI GCGC 2 cut(s) 626, 1169
Hin1II CATG 7 cut(s) 295, 427, 458, 686, 792, 1057, 1114
Hin6I GCGC 2 cut(s) 624, 1167
HinP1I GCGC 2 cut(s) 624, 1167
HinfI GANTC 2 cut(s) 240, 461
HpaII CCGG 1 cut(s) 646
HphI GGTGA 3 cut(s) 31, 538, 1082
Hpy166II GTNNAC 3 cut(s) 691, 917, 1087
Hpy188I TCNGA 3 cut(s) 236, 267, 605
Hpy188III TCNNGA 2 cut(s) 479, 1036
Hpy8I GTNNAC 3 cut(s) 691, 917, 1087
HpyAV CCTTC 4 cut(s) 505, 907, 993, 1042
HpyCH4III ACNGT 2 cut(s) 217, 749
HpyCH4IV ACGT 3 cut(s) 499, 661, 1041
HpyCH4V TGCA 6 cut(s) 374, 387, 788, 792, 797, 963
HpyF10VI GCNNNNNNNGC 6 cut(s) 449, 605, 630, 683, 794, 889
HpyF3I CTNAG 7 cut(s) 347, 602, 634, 773, 879, 939, 1141
HpySE526I ACGT 3 cut(s) 499, 661, 1041
Hsp92II CATG 7 cut(s) 295, 427, 458, 686, 792, 1057, 1114
HspAI GCGC 2 cut(s) 624, 1167
Ksp22I TGATCA 3 cut(s) 94, 274, 310
Kzo9I GATC 7 cut(s) 94, 267, 274, 310, 529, 709, 840
LguI GCTCTTC 1 cut(s) 1167
LmnI GCTCC 3 cut(s) 109, 897, 1102
Lsp1109I GCAGC 1 cut(s) 739
LweI GCATC 6 cut(s) 360, 396, 397, 484, 775, 1120
MaeI CTAG 3 cut(s) 200, 335, 929
MaeII ACGT 3 cut(s) 499, 661, 1041
MaeIII GTNAC 1 cut(s) 559
MalI GATC 7 cut(s) 96, 269, 276, 312, 531, 711, 842
MboI GATC 7 cut(s) 94, 267, 274, 310, 529, 709, 840
MboII GAAGA 7 cut(s) 39, 59, 124, 650, 839, 940, 1184
MfeI CAATTG 1 cut(s) 375
MhlI GDGCHC 2 cut(s) 894, 1179
MluCI AATT 5 cut(s) 98, 140, 375, 818, 1118
MmeI TCCRAC 3 cut(s) 77, 214, 489
Mph1103I ATGCAT 2 cut(s) 389, 790
MseI TTAA 2 cut(s) 212, 1189
MspI CCGG 1 cut(s) 646
MspR9I CCNGG 1 cut(s) 550
MunI CAATTG 1 cut(s) 375
MvaI CCWGG 1 cut(s) 550
MwoI GCNNNNNNNGC 6 cut(s) 449, 605, 630, 683, 794, 889
NcoI CCATGG 1 cut(s) 454
NdeI CATATG 2 cut(s) 526, 799
NdeII GATC 7 cut(s) 94, 267, 274, 310, 529, 709, 840
NlaIII CATG 7 cut(s) 295, 427, 458, 686, 792, 1057, 1114
NlaIV GGNNCC 3 cut(s) 341, 766, 893
NmeAIII GCCGAG 1 cut(s) 1159
NmuCI GTSAC 1 cut(s) 559
NsiI ATGCAT 2 cut(s) 389, 790
NspI RCATGY 1 cut(s) 792
PaeI GCATGC 1 cut(s) 792
PceI AGGCCT 1 cut(s) 1003
PciSI GCTCTTC 1 cut(s) 1167
PcsI WCGNNNNNNNCGW 1 cut(s) 658
PfeI GAWTC 2 cut(s) 240, 461
PflFI GACNNNGTC 1 cut(s) 854
PkrI GCNGC 2 cut(s) 445, 754
Ppu21I YACGTR 1 cut(s) 662
PpuMI RGGWCCY 1 cut(s) 764
Psp5II RGGWCCY 1 cut(s) 764
Psp6I CCWGG 1 cut(s) 548
PspGI CCWGG 1 cut(s) 548
PspN4I GGNNCC 3 cut(s) 341, 766, 893
PspPI GGNCC 5 cut(s) 127, 331, 340, 679, 764
PspPPI RGGWCCY 1 cut(s) 764
PsyI GACNNNGTC 1 cut(s) 854
RsaI GTAC 1 cut(s) 778
RsaNI GTAC 1 cut(s) 777
SapI GCTCTTC 1 cut(s) 1167
SaqAI TTAA 2 cut(s) 212, 1189
SatI GCNGC 2 cut(s) 444, 753
Sau3AI GATC 7 cut(s) 94, 267, 274, 310, 529, 709, 840
Sau96I GGNCC 5 cut(s) 127, 331, 340, 679, 764
ScaI AGTACT 1 cut(s) 778
ScrFI CCNGG 1 cut(s) 550
SduI GDGCHC 2 cut(s) 894, 1179
SfaNI GCATC 6 cut(s) 360, 396, 397, 484, 775, 1120
SfcI CTRYAG 2 cut(s) 627, 866
SinI GGWCC 5 cut(s) 127, 331, 340, 679, 764
SphI GCATGC 1 cut(s) 792
Sse9I AATT 5 cut(s) 98, 140, 375, 818, 1118
SseBI AGGCCT 1 cut(s) 1003
SsiI CCGC 2 cut(s) 443, 677
SspMI CTAG 3 cut(s) 200, 335, 929
StuI AGGCCT 1 cut(s) 1003
StyD4I CCNGG 1 cut(s) 548
StyI CCWWGG 4 cut(s) 27, 334, 454, 701
TaaI ACNGT 2 cut(s) 217, 749
TaiI ACGT 3 cut(s) 502, 664, 1044
TaqI TCGA 1 cut(s) 843
TasI AATT 5 cut(s) 98, 140, 375, 818, 1118
TatI WGTACW 1 cut(s) 776
TauI GCSGC 1 cut(s) 446
TfiI GAWTC 2 cut(s) 240, 461
Tru1I TTAA 2 cut(s) 212, 1189
Tru9I TTAA 2 cut(s) 212, 1189
TscAI CASTG 5 cut(s) 513, 544, 586, 799, 978
TseFI GTSAC 1 cut(s) 559
TseI GCWGC 1 cut(s) 752
Tsp45I GTSAC 1 cut(s) 559
TspDTI ATGAA 4 cut(s) 17, 280, 412, 1042
TspRI CASTG 5 cut(s) 513, 544, 586, 799, 978
Tth111I GACNNNGTC 1 cut(s) 854
VpaK11BI GGWCC 5 cut(s) 127, 331, 340, 679, 764
XapI RAATTY 2 cut(s) 140, 1118
XceI RCATGY 1 cut(s) 792
XmaJI CCTAGG 1 cut(s) 334
XmiI GTMKAC 2 cut(s) 916, 1086
XspI CTAG 3 cut(s) 200, 335, 929
ZrmI AGTACT 1 cut(s) 778
Zsp2I ATGCAT 2 cut(s) 389, 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.