RchiOBHm_Chr4g0417441

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000622
Physical Location & Seq
Forward (+)
42703322 .. 42704305
984 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38753

Sequence Viewer

Length: 921 bp
ATGAAGTTAGAAGCTGAAGTAATCTCCAAACAGATTATCACTCCTTCTTCTCCAACCCCTGACCATCTTCGCCATTACCAGTTCTCATTTCTTGATCAAGTATCTCCCCCGGTCTATAACCCTTTGCTCCTCTTTTATGAATTCAATGCCAAAACTCAACCCAACATCACTGAAATATCCAACCACCTGAAGACCTCCTTAGCCAAAGTCTTAACCCTTTTCTACCCACTAGCCGGACGACTCAATGGCAACCAACACGTGGATTGCAACGACAATGGTATACCCTACCTTGAAGCTCAAGTGAACTGCAAACTCTCAGATGTTCTCGACAATCCGATACCCGGAGAACTCAACAAGCTCATGCCATTTGAACTGGACGATGTGGCAAACAAATTTGCCCTTGGTATCCAGCTCAACATCTTTCAATGTGGTGGTTTTGCTATTGCTCAGTGCCTTTCTCACAAGCTCGCAGATGGGTTATCATACTTCACGTTCAGCAAAACTTGGGCCGCCACTGCCCGTGGCGAACCCAACATAGCACATCCGCAGTTCATTTCCGCCACACTATTCCCTCCCAAGAATTTAAGTGGATTTGATGCGCGCACTGGTATCACAAGAAACAAAGTGACAAAACGCTTTGTGTTTAGTGCCTCTAACATAGAGGAGCTCAGAAAAGTTCAAGCACATAAGAACGTAACACGCCCTTCACGTGTTGAGGCCTTATCTTGTTTCATCTTGAGCCGATTTATGGAGGCTACCAAAGATATTGGATCAGCTGACAAGTTTTACAGGGTGGTTCATGCTGTGAACTTGCGGCCGAGGTTCGACCCACCACTGCCGCACCATTCCTTTGGGAACCTTTACCGAATAGCCATGACAGCACCGTTACAAATAAGTTCCAGGGAACCATATCATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

306

Amino Acids

34.34

Weight (kDa)

9.02

Isoelectric Point (pI)

41.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 6 - 291 6.5e-57 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24710 FvH4_4g24720 FvH4_4g24770 FvH4_4g24771 FvH4_4g24772 FvH4_5g39380
malus_domestica MD10G1199500.v1.1 MD13G1109100.v1.1 MD13G1109200.v1.1 MD13G1114400.v1.1 MD13G1114800.v1.1 MD16G1108800.v1.1
prunus_persica Prupe.1G235900_v2.0.a1 Prupe.1G236600_v2.0.a1 Prupe.1G236700_v2.0.a1 Prupe.1G236800_v2.0.a1 Prupe.4G142000_v2.0.a1
pyrus_communis pycom10g17180 pycom13g09460 pycom13g09930 pycom13g09940
rosa_chinensis RchiOBHm_Chr3g0490281 RchiOBHm_Chr3g0490291 RchiOBHm_Chr3g0490301 RchiOBHm_Chr4g0417441 RchiOBHm_Chr4g0417461 RchiOBHm_Chr4g0431811 RchiOBHm_Chr4g0431821 RchiOBHm_Chr4g0431851
rosa_laevigata RLG00000006882 RLG00000006886 RLG00000006887 RLG00000007905 RLG00000007911 RLG00000007913 RLG00000029538
rosa_multiflora Rmu_sc0001459.1_g000008 Rmu_sc0001459.1_g000011 Rmu_sc0002489.1_g000020 Rmu_sc0002489.1_g000024 Rmu_sc0002489.1_g000027 Rmu_sc0002489.1_g000028 Rmu_sc0002489.1_g000029 Rmu_sc0004142.1_g000001 Rmu_sc0004142.1_g000024 Rmu_sc0011237.1_g000018 Rmu_sc0031439.1_g000001 Rmu_sc0031677.1_g000001 Rmu_ssc0000361.1_g000005
rosa_roxburghii Rroxscaffold_3G00262760 Rroxscaffold_4G00317310 Rroxscaffold_5G00361150 Rroxscaffold_5G00373190 Rroxscaffold_5G00373210 Rroxscaffold_5G00373220 Rroxscaffold_5G00373240
rosa_rugosa Rorug01G0111100 Rorug04G0252400 Rorug04G0252400 Rorug04G0252400 Rorug04G0252600
rosa_samantha Rh1BG102300 Rh3AG292200 Rh3AG292300 Rh4AG309100 Rh4AG309300 Rh4AG309500 Rh6BG200900
rosa_wichuraiana Rw0G002990 Rw0G003000 Rw0G019170 Rw3G025890 Rw3G025900 Rw4G017830 Rw4G026810 Rw4G026870 Rw4G026880 Rw4G026920 Rw6G017190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 259, 914
AccI GTMKAC 1 cut(s) 280
AccII CGCG 1 cut(s) 601
AciI CCGC 5 cut(s) 510, 545, 558, 814, 839
AclWI GGATC 1 cut(s) 778
AcoI YGGCCR 1 cut(s) 815
AcsI RAATTY 3 cut(s) 140, 392, 580
AcuI CTGAAG 2 cut(s) 36, 209
AcvI CACGTG 2 cut(s) 259, 710
AfiI CCNNNNNNNGG 5 cut(s) 233, 259, 341, 748, 914
AflIII ACRYGT 2 cut(s) 256, 709
AgsI TTSAA 5 cut(s) 145, 293, 371, 425, 680
AjnI CCWGG 1 cut(s) 899
AluBI AGCT 7 cut(s) 14, 296, 358, 412, 466, 667, 776
AluI AGCT 7 cut(s) 14, 296, 358, 412, 466, 667, 776
Alw21I GWGCWC 1 cut(s) 669
AlwI GGATC 1 cut(s) 778
AoxI GGCC 3 cut(s) 507, 717, 815
ApoI RAATTY 3 cut(s) 140, 392, 580
AspLEI GCGC 2 cut(s) 601, 603
AspS9I GGNCC 1 cut(s) 507
AsuC2I CCSGG 2 cut(s) 110, 342
BanII GRGCYC 1 cut(s) 669
BbrPI CACGTG 2 cut(s) 259, 710
BbsI GAAGAC 1 cut(s) 197
Bbv12I GWGCWC 1 cut(s) 669
BccI CCATC 2 cut(s) 72, 467
BciT130I CCWGG 1 cut(s) 901
BciVI GTATCC 1 cut(s) 416
BclI TGATCA 1 cut(s) 94
BcnI CCSGG 2 cut(s) 110, 342
BfaI CTAG 1 cut(s) 230
BfuI GTATCC 1 cut(s) 416
BisI GCNGC 3 cut(s) 510, 815, 839
BlsI GCNGC 3 cut(s) 511, 816, 840
Bme1390I CCNGG 3 cut(s) 110, 342, 901
BmgT120I GGNCC 1 cut(s) 507
BmiI GGNNCC 2 cut(s) 857, 906
BmrFI CCNGG 3 cut(s) 110, 342, 901
BmsI GCATC 1 cut(s) 586
BpiI GAAGAC 1 cut(s) 197
Bpu10I CCTNAGC 1 cut(s) 199
BpuEI CTTGAG 2 cut(s) 282, 757
BpuMI CCSGG 2 cut(s) 110, 342
BsaAI YACGTR 2 cut(s) 259, 710
BsaJI CCNNGG 5 cut(s) 108, 400, 520, 818, 900
Bsc4I CCNNNNNNNGG 5 cut(s) 233, 259, 341, 748, 914
Bse1I ACTGG 3 cut(s) 79, 378, 610
BseBI CCWGG 1 cut(s) 901
BseDI CCNNGG 5 cut(s) 108, 400, 520, 818, 900
BseGI GGATG 1 cut(s) 541
BseLI CCNNNNNNNGG 5 cut(s) 233, 259, 341, 748, 914
BseMII CTCAG 3 cut(s) 330, 461, 682
BseNI ACTGG 3 cut(s) 79, 378, 610
BsePI GCGCGC 1 cut(s) 599
BseRI GAGGAG 2 cut(s) 119, 677
BseX3I CGGCCG 1 cut(s) 815
Bsh1236I CGCG 1 cut(s) 601
Bsh1285I CGRYCG 1 cut(s) 818
BshFI GGCC 3 cut(s) 509, 719, 817
BsiEI CGRYCG 1 cut(s) 818
BsiHKAI GWGCWC 1 cut(s) 669
BsiSI CCGG 3 cut(s) 110, 234, 342
BslI CCNNNNNNNGG 5 cut(s) 233, 259, 341, 748, 914
BsnI GGCC 3 cut(s) 509, 719, 817
Bsp1286I GDGCHC 1 cut(s) 669
Bsp143I GATC 2 cut(s) 94, 770
BspACI CCGC 5 cut(s) 510, 545, 558, 814, 839
BspANI GGCC 3 cut(s) 509, 719, 817
BspCNI CTCAG 3 cut(s) 329, 460, 681
BspFNI CGCG 1 cut(s) 601
BspLI GGNNCC 2 cut(s) 857, 906
BspPI GGATC 1 cut(s) 778
BsrI ACTGG 3 cut(s) 79, 378, 610
BssECI CCNNGG 5 cut(s) 108, 400, 520, 818, 900
BssHII GCGCGC 1 cut(s) 599
BssMI GATC 2 cut(s) 94, 770
BssNAI GTATAC 1 cut(s) 281
BssT1I CCWWGG 1 cut(s) 400
Bst1107I GTATAC 1 cut(s) 281
Bst2UI CCWGG 1 cut(s) 901
Bst4CI ACNGT 1 cut(s) 885
BstBAI YACGTR 2 cut(s) 259, 710
BstC8I GCNNGC 2 cut(s) 468, 601
BstDEI CTNAG 4 cut(s) 199, 316, 447, 668
BstDSI CCRYGG 1 cut(s) 520
BstF5I GGATG 1 cut(s) 541
BstFNI CGCG 1 cut(s) 601
BstHHI GCGC 2 cut(s) 601, 603
BstKTI GATC 2 cut(s) 97, 773
BstMBI GATC 2 cut(s) 94, 770
BstMCI CGRYCG 1 cut(s) 818
BstMWI GCNNNNNNNGC 2 cut(s) 515, 878
BstNI CCWGG 1 cut(s) 901
BstSCI CCNGG 3 cut(s) 108, 340, 899
BstUI CGCG 1 cut(s) 601
BstV2I GAAGAC 1 cut(s) 197
BstXI CCANNNNNNTGG 1 cut(s) 851
BstZ17I GTATAC 1 cut(s) 281
BstZI CGGCCG 1 cut(s) 815
BsuI GTATCC 1 cut(s) 416
BsuRI GGCC 3 cut(s) 509, 719, 817
BtgI CCRYGG 1 cut(s) 520
BtsCI GGATG 1 cut(s) 541
BtsI GCAGTG 2 cut(s) 513, 833
BtsIMutI CAGTG 5 cut(s) 168, 455, 513, 603, 833
Cac8I GCNNGC 2 cut(s) 468, 601
CfoI GCGC 2 cut(s) 601, 603
Cfr13I GGNCC 1 cut(s) 507
CviAII CATG 4 cut(s) 361, 800, 874, 914
DdeI CTNAG 4 cut(s) 199, 316, 447, 668
DpnI GATC 2 cut(s) 96, 772
DpnII GATC 2 cut(s) 94, 770
EaeI YGGCCR 1 cut(s) 815
EagI CGGCCG 1 cut(s) 815
EciI GGCGGA 1 cut(s) 547
Ecl136II GAGCTC 1 cut(s) 667
EclXI CGGCCG 1 cut(s) 815
Eco130I CCWWGG 1 cut(s) 400
Eco147I AGGCCT 1 cut(s) 719
Eco24I GRGCYC 1 cut(s) 669
Eco52I CGGCCG 1 cut(s) 815
Eco53kI GAGCTC 1 cut(s) 667
Eco57I CTGAAG 2 cut(s) 36, 209
Eco72I CACGTG 2 cut(s) 259, 710
EcoICRI GAGCTC 1 cut(s) 667
EcoRI GAATTC 1 cut(s) 140
EcoRII CCWGG 1 cut(s) 899
EcoT14I CCWWGG 1 cut(s) 400
EcoT38I GRGCYC 1 cut(s) 669
ErhI CCWWGG 1 cut(s) 400
FaeI CATG 4 cut(s) 364, 803, 877, 917
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FatI CATG 4 cut(s) 360, 799, 873, 913
FbaI TGATCA 1 cut(s) 94
FblI GTMKAC 1 cut(s) 280
Fnu4HI GCNGC 3 cut(s) 510, 815, 839
FokI GGATG 1 cut(s) 528
FriOI GRGCYC 1 cut(s) 669
Fsp4HI GCNGC 3 cut(s) 510, 815, 839
FspBI CTAG 1 cut(s) 230
GlaI GCGC 2 cut(s) 600, 602
GluI GCNGC 3 cut(s) 510, 815, 839
HaeIII GGCC 3 cut(s) 509, 719, 817
HapII CCGG 3 cut(s) 110, 234, 342
HhaI GCGC 2 cut(s) 601, 603
Hin1II CATG 4 cut(s) 364, 803, 877, 917
Hin6I GCGC 2 cut(s) 599, 601
HinP1I GCGC 2 cut(s) 599, 601
HinfI GANTC 1 cut(s) 240
HpaII CCGG 3 cut(s) 110, 234, 342
Hpy166II GTNNAC 3 cut(s) 281, 304, 808
Hpy188I TCNGA 3 cut(s) 319, 336, 671
Hpy188III TCNNGA 3 cut(s) 92, 326, 736
Hpy8I GTNNAC 3 cut(s) 281, 304, 808
HpyAV CCTTC 2 cut(s) 54, 714
HpyCH4III ACNGT 1 cut(s) 885
HpyCH4IV ACGT 4 cut(s) 258, 491, 693, 709
HpyCH4V TGCA 2 cut(s) 267, 309
HpyF10VI GCNNNNNNNGC 2 cut(s) 515, 878
HpyF3I CTNAG 4 cut(s) 199, 316, 447, 668
HpySE526I ACGT 4 cut(s) 258, 491, 693, 709
Hsp92II CATG 4 cut(s) 364, 803, 877, 917
HspAI GCGC 2 cut(s) 599, 601
Ksp22I TGATCA 1 cut(s) 94
Kzo9I GATC 2 cut(s) 94, 770
LmnI GCTCC 2 cut(s) 132, 664
LweI GCATC 1 cut(s) 586
MaeI CTAG 1 cut(s) 230
MaeII ACGT 4 cut(s) 258, 491, 693, 709
MaeIII GTNAC 3 cut(s) 625, 694, 885
MalI GATC 2 cut(s) 96, 772
MboI GATC 2 cut(s) 94, 770
MboII GAAGA 3 cut(s) 39, 59, 202
MhlI GDGCHC 1 cut(s) 669
MluCI AATT 3 cut(s) 140, 392, 580
MlyI GAGTC 1 cut(s) 234
MmeI TCCRAC 2 cut(s) 77, 204
MnlI CCTC 8 cut(s) 140, 205, 582, 655, 661, 709, 745, 813
MseI TTAA 2 cut(s) 212, 584
MspA1I CMGCKG 1 cut(s) 776
MspI CCGG 3 cut(s) 110, 234, 342
MspR9I CCNGG 3 cut(s) 110, 342, 901
MvaI CCWGG 1 cut(s) 901
MvnI CGCG 1 cut(s) 601
MwoI GCNNNNNNNGC 2 cut(s) 515, 878
NciI CCSGG 2 cut(s) 110, 342
NdeII GATC 2 cut(s) 94, 770
NlaIII CATG 4 cut(s) 364, 803, 877, 917
NlaIV GGNNCC 2 cut(s) 857, 906
NmeAIII GCCGAG 1 cut(s) 843
NmuCI GTSAC 1 cut(s) 625
PauI GCGCGC 1 cut(s) 599
PceI AGGCCT 1 cut(s) 719
PcsI WCGNNNNNNNCGW 1 cut(s) 706
PflMI CCANNNNNTGG 2 cut(s) 259, 914
PkrI GCNGC 3 cut(s) 511, 816, 840
PleI GAGTC 1 cut(s) 234
PmaCI CACGTG 2 cut(s) 259, 710
PmlI CACGTG 2 cut(s) 259, 710
PpsI GAGTC 1 cut(s) 234
Ppu21I YACGTR 2 cut(s) 259, 710
Psp124BI GAGCTC 1 cut(s) 669
Psp6I CCWGG 1 cut(s) 899
PspCI CACGTG 2 cut(s) 259, 710
PspGI CCWGG 1 cut(s) 899
PspN4I GGNNCC 2 cut(s) 857, 906
PspPI GGNCC 1 cut(s) 507
PteI GCGCGC 1 cut(s) 599
PvuII CAGCTG 1 cut(s) 776
SacI GAGCTC 1 cut(s) 669
SaqAI TTAA 2 cut(s) 212, 584
SatI GCNGC 3 cut(s) 510, 815, 839
Sau3AI GATC 2 cut(s) 94, 770
Sau96I GGNCC 1 cut(s) 507
SchI GAGTC 1 cut(s) 234
ScrFI CCNGG 3 cut(s) 110, 342, 901
SduI GDGCHC 1 cut(s) 669
SfaNI GCATC 1 cut(s) 586
SmlI CTYRAG 2 cut(s) 297, 736
SmoI CTYRAG 2 cut(s) 297, 736
Sse9I AATT 3 cut(s) 140, 392, 580
SseBI AGGCCT 1 cut(s) 719
SsiI CCGC 5 cut(s) 510, 545, 558, 814, 839
SspMI CTAG 1 cut(s) 230
SstI GAGCTC 1 cut(s) 669
StuI AGGCCT 1 cut(s) 719
StyD4I CCNGG 3 cut(s) 108, 340, 899
StyI CCWWGG 1 cut(s) 400
TaaI ACNGT 1 cut(s) 885
TaiI ACGT 4 cut(s) 261, 494, 696, 712
TaqI TCGA 2 cut(s) 327, 825
TasI AATT 3 cut(s) 140, 392, 580
TauI GCSGC 3 cut(s) 512, 817, 841
Tru1I TTAA 2 cut(s) 212, 584
Tru9I TTAA 2 cut(s) 212, 584
TscAI CASTG 5 cut(s) 175, 455, 520, 610, 840
TseFI GTSAC 1 cut(s) 625
Tsp45I GTSAC 1 cut(s) 625
TspDTI ATGAA 5 cut(s) 17, 153, 541, 721, 788
TspRI CASTG 5 cut(s) 175, 455, 520, 610, 840
Van91I CCANNNNNTGG 2 cut(s) 259, 914
XapI RAATTY 3 cut(s) 140, 392, 580
XmiI GTMKAC 1 cut(s) 280
XspI CTAG 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.