Rroxscaffold_5G00373190

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000622
Physical Location & Seq
Reverse (-)
54446847 .. 54449972
3126 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00373190.1

Sequence Viewer

Length: 501 bp
ATGGATCTCCGTTCGAGGTTTGACTCACCTCTGCCGCAGAATAGTTTTGGGAACTATTACCGGGCAGCCACTGCAACTCCAACATTGGTTGATGGGGAGGAGAGAGGTGGCCTTGTGAGGCAGGTGATCGAGGAAATAGAGAAGATTGATAGTAAGTATATGAGGAAATTTCAAGATGGCTATAAGGAACATTTGGATTTCATGAGGAGAAGGATGGAAAGGGCTGCAAAAGGAGAGCTTGTTACAGTGACGTTTTCGAGTCTCTGCAGGTTTCCATTGTATGAGGCTGATTTTGGTTGGGGGAAGCCAGCGTGGGTGAGCATGGCTTCCATGAGAATCAGCAACCAAATAGTTTTCATGGACACCAAATTGGGTGATGGAATAGAGTCGTATTTTAGCTTCAAGGAGGAAGACATGGCCAAGTTTGAACTTGACTCGGAGTTCCTCGAGTTGATTTCTCCAATTGGTAATGTCAAGAAAAATCCATTTGCACGTCTTTAA

Protein Analysis

166

Amino Acids

19.22

Weight (kDa)

5.59

Isoelectric Point (pI)

48.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 1 - 148 1.6e-21 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24710 FvH4_4g24720 FvH4_4g24770 FvH4_4g24771 FvH4_4g24772 FvH4_5g39380
malus_domestica MD10G1199500.v1.1 MD13G1109100.v1.1 MD13G1109200.v1.1 MD13G1114400.v1.1 MD13G1114800.v1.1 MD16G1108800.v1.1
prunus_persica Prupe.1G235900_v2.0.a1 Prupe.1G236600_v2.0.a1 Prupe.1G236700_v2.0.a1 Prupe.1G236800_v2.0.a1 Prupe.4G142000_v2.0.a1
pyrus_communis pycom10g17180 pycom13g09460 pycom13g09930 pycom13g09940
rosa_chinensis RchiOBHm_Chr3g0490281 RchiOBHm_Chr3g0490291 RchiOBHm_Chr3g0490301 RchiOBHm_Chr4g0417441 RchiOBHm_Chr4g0417461 RchiOBHm_Chr4g0431811 RchiOBHm_Chr4g0431821 RchiOBHm_Chr4g0431851
rosa_laevigata RLG00000006882 RLG00000006886 RLG00000006887 RLG00000007905 RLG00000007911 RLG00000007913 RLG00000029538
rosa_multiflora Rmu_sc0001459.1_g000008 Rmu_sc0001459.1_g000011 Rmu_sc0002489.1_g000020 Rmu_sc0002489.1_g000024 Rmu_sc0002489.1_g000027 Rmu_sc0002489.1_g000028 Rmu_sc0002489.1_g000029 Rmu_sc0004142.1_g000001 Rmu_sc0004142.1_g000024 Rmu_sc0011237.1_g000018 Rmu_sc0031439.1_g000001 Rmu_sc0031677.1_g000001 Rmu_ssc0000361.1_g000005
rosa_roxburghii Rroxscaffold_3G00262760 Rroxscaffold_4G00317310 Rroxscaffold_5G00361150 Rroxscaffold_5G00373190 Rroxscaffold_5G00373210 Rroxscaffold_5G00373220 Rroxscaffold_5G00373240
rosa_rugosa Rorug01G0111100 Rorug04G0252400 Rorug04G0252400 Rorug04G0252400 Rorug04G0252600
rosa_samantha Rh1BG102300 Rh3AG292200 Rh3AG292300 Rh4AG309100 Rh4AG309300 Rh4AG309500 Rh6BG200900
rosa_wichuraiana Rw0G002990 Rw0G003000 Rw0G019170 Rw3G025890 Rw3G025900 Rw4G017830 Rw4G026810 Rw4G026870 Rw4G026880 Rw4G026920 Rw6G017190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 112
Acc36I ACCTGC 2 cut(s) 112, 258
AciI CCGC 1 cut(s) 35
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 417
AcsI RAATTY 1 cut(s) 167
AgsI TTSAA 3 cut(s) 173, 403, 428
AjiI CACGTC 1 cut(s) 494
AluBI AGCT 2 cut(s) 238, 399
AluI AGCT 2 cut(s) 238, 399
Alw26I GTCTC 1 cut(s) 266
AlwI GGATC 1 cut(s) 12
AlwNI CAGNNNCTG 1 cut(s) 71
Ama87I CYCGRG 1 cut(s) 446
AoxI GGCC 2 cut(s) 109, 417
ApeKI GCWGC 2 cut(s) 65, 224
ApoI RAATTY 1 cut(s) 167
AsuC2I CCSGG 1 cut(s) 62
AsuHPI GGTGA 4 cut(s) 18, 136, 328, 386
AvaI CYCGRG 1 cut(s) 446
BalI TGGCCA 1 cut(s) 419
BarI GAAGNNNNNNTAC 2 cut(s) 383, 415
BbsI GAAGAC 1 cut(s) 417
BbvI GCAGC 2 cut(s) 77, 211
BccI CCATC 4 cut(s) 86, 170, 208, 371
BcnI CCSGG 1 cut(s) 62
BcoDI GTCTC 1 cut(s) 266
BfmI CTRYAG 1 cut(s) 265
BfuAI ACCTGC 2 cut(s) 112, 258
BisI GCNGC 3 cut(s) 35, 66, 225
BlsI GCNGC 3 cut(s) 36, 67, 226
Bme1390I CCNGG 1 cut(s) 62
BmeT110I CYCGRG 1 cut(s) 446
BmgBI CACGTC 1 cut(s) 494
BmrFI CCNGG 1 cut(s) 62
BpiI GAAGAC 1 cut(s) 417
BpuMI CCSGG 1 cut(s) 62
BsaXI ACNNNNNCTCC 4 cut(s) 61, 91, 225, 255
BseGI GGATG 1 cut(s) 219
BseRI GAGGAG 2 cut(s) 113, 220
BseXI GCAGC 2 cut(s) 77, 211
BshFI GGCC 2 cut(s) 111, 419
BsiHKCI CYCGRG 1 cut(s) 446
BsiSI CCGG 1 cut(s) 61
BsmAI GTCTC 1 cut(s) 266
BsnI GGCC 2 cut(s) 111, 419
BsoBI CYCGRG 1 cut(s) 446
Bsp143I GATC 2 cut(s) 4, 126
BspACI CCGC 1 cut(s) 35
BspANI GGCC 2 cut(s) 111, 419
BspHI TCATGA 1 cut(s) 201
BspMAI CTGCAG 1 cut(s) 269
BspMI ACCTGC 2 cut(s) 112, 258
BspPI GGATC 1 cut(s) 12
BssMI GATC 2 cut(s) 4, 126
Bst4CI ACNGT 1 cut(s) 247
BstAPI GCANNNNNTGC 1 cut(s) 71
BstC8I GCNNGC 1 cut(s) 309
BstF5I GGATG 1 cut(s) 219
BstKTI GATC 2 cut(s) 7, 129
BstMAI GTCTC 1 cut(s) 266
BstMBI GATC 2 cut(s) 4, 126
BstMWI GCNNNNNNNGC 1 cut(s) 71
BstSCI CCNGG 1 cut(s) 60
BstSFI CTRYAG 1 cut(s) 265
BstV1I GCAGC 2 cut(s) 77, 211
BstV2I GAAGAC 1 cut(s) 417
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 2 cut(s) 111, 419
BtrI CACGTC 1 cut(s) 494
BtsCI GGATG 1 cut(s) 219
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 2 cut(s) 69, 252
BveI ACCTGC 2 cut(s) 112, 258
Cac8I GCNNGC 1 cut(s) 309
CaiI CAGNNNCTG 1 cut(s) 71
CciI TCATGA 1 cut(s) 201
CviAII CATG 5 cut(s) 202, 322, 331, 358, 415
DpnI GATC 2 cut(s) 6, 128
DpnII GATC 2 cut(s) 4, 126
EaeI YGGCCR 1 cut(s) 417
Eco88I CYCGRG 1 cut(s) 446
FaeI CATG 5 cut(s) 205, 325, 334, 361, 418
FaiI YATR 9 cut(s) 159, 161, 183, 203, 282, 323, 332, 359, 416
FalI AAGNNNNNCTT 2 cut(s) 222, 254
FatI CATG 5 cut(s) 201, 321, 330, 357, 414
Fnu4HI GCNGC 3 cut(s) 35, 66, 225
FokI GGATG 1 cut(s) 226
Fsp4HI GCNGC 3 cut(s) 35, 66, 225
GluI GCNGC 3 cut(s) 35, 66, 225
HaeIII GGCC 2 cut(s) 111, 419
HapII CCGG 1 cut(s) 61
Hin1II CATG 5 cut(s) 205, 325, 334, 361, 418
HinfI GANTC 5 cut(s) 23, 259, 336, 386, 434
HpaII CCGG 1 cut(s) 61
HphI GGTGA 4 cut(s) 18, 136, 328, 386
Hpy188I TCNGA 1 cut(s) 439
Hpy188III TCNNGA 3 cut(s) 173, 202, 475
HpyAV CCTTC 1 cut(s) 204
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4IV ACGT 2 cut(s) 251, 493
HpyCH4V TGCA 4 cut(s) 74, 227, 267, 491
HpyF10VI GCNNNNNNNGC 1 cut(s) 71
HpySE526I ACGT 2 cut(s) 251, 493
Hsp92II CATG 5 cut(s) 205, 325, 334, 361, 418
Kzo9I GATC 2 cut(s) 4, 126
LpnPI CCDG 4 cut(s) 74, 107, 253, 321
Lsp1109I GCAGC 2 cut(s) 77, 211
MaeII ACGT 2 cut(s) 251, 493
MaeIII GTNAC 2 cut(s) 241, 247
MalI GATC 2 cut(s) 6, 128
MboI GATC 2 cut(s) 4, 126
MboII GAAGA 2 cut(s) 154, 422
MfeI CAATTG 1 cut(s) 462
MflI RGATCY 1 cut(s) 4
MlsI TGGCCA 1 cut(s) 419
MluCI AATT 3 cut(s) 167, 368, 462
MluNI TGGCCA 1 cut(s) 419
MlyI GAGTC 4 cut(s) 17, 268, 395, 428
MmeI TCCRAC 1 cut(s) 104
Mox20I TGGCCA 1 cut(s) 419
MscI TGGCCA 1 cut(s) 419
MseI TTAA 1 cut(s) 499
Msp20I TGGCCA 1 cut(s) 419
MspI CCGG 1 cut(s) 61
MspR9I CCNGG 1 cut(s) 62
MunI CAATTG 1 cut(s) 462
MwoI GCNNNNNNNGC 1 cut(s) 71
NciI CCSGG 1 cut(s) 62
NdeII GATC 2 cut(s) 4, 126
NlaIII CATG 5 cut(s) 205, 325, 334, 361, 418
NmuCI GTSAC 1 cut(s) 247
PaeR7I CTCGAG 1 cut(s) 446
PagI TCATGA 1 cut(s) 201
PaqCI CACCTGC 1 cut(s) 112
PfeI GAWTC 1 cut(s) 336
PkrI GCNGC 3 cut(s) 36, 67, 226
PleI GAGTC 4 cut(s) 17, 267, 394, 428
PpsI GAGTC 4 cut(s) 17, 267, 394, 428
PspXI VCTCGAGB 1 cut(s) 446
PstI CTGCAG 1 cut(s) 269
PstNI CAGNNNCTG 1 cut(s) 71
PsuI RGATCY 1 cut(s) 4
SaqAI TTAA 1 cut(s) 499
SatI GCNGC 3 cut(s) 35, 66, 225
Sau3AI GATC 2 cut(s) 4, 126
SchI GAGTC 4 cut(s) 17, 268, 395, 428
ScrFI CCNGG 1 cut(s) 62
SetI ASST 9 cut(s) 20, 31, 109, 126, 240, 254, 272, 401, 496
SfcI CTRYAG 1 cut(s) 265
Sfr274I CTCGAG 1 cut(s) 446
SlaI CTCGAG 1 cut(s) 446
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
Sse9I AATT 3 cut(s) 167, 368, 462
SsiI CCGC 1 cut(s) 35
StyD4I CCNGG 1 cut(s) 60
TaaI ACNGT 1 cut(s) 247
TaiI ACGT 2 cut(s) 254, 496
TaqI TCGA 4 cut(s) 14, 129, 257, 447
TasI AATT 3 cut(s) 167, 368, 462
TauI GCSGC 1 cut(s) 37
TfiI GAWTC 1 cut(s) 336
Tru1I TTAA 1 cut(s) 499
Tru9I TTAA 1 cut(s) 499
TscAI CASTG 2 cut(s) 76, 252
TseFI GTSAC 1 cut(s) 247
TseI GCWGC 2 cut(s) 65, 224
Tsp45I GTSAC 1 cut(s) 247
TspDTI ATGAA 2 cut(s) 190, 346
TspRI CASTG 2 cut(s) 76, 252
XapI RAATTY 1 cut(s) 167
XhoI CTCGAG 1 cut(s) 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.