Rmu_sc0002489.1_g000020

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002489.1
OG0000622
Physical Location & Seq
Reverse (-)
65430 .. 66753
1324 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002489.1_g000020.1.cds

Sequence Viewer

Length: 1107 bp
atgaaggttgaagtagaagtaatctccaaggagattagcacccaatcttctccgacccctgcttgtcttcgccattaccagctctcctcctttgatcaattagctcccccagtctataaccctttggtcctcttctacgaattcaatgacaacacagcccccaacattactgaaatatccagccacctcaaaaagtccctagccgaagtcttaaccgttttctacccattagccggacgaatcaaacacgacgactggcatgttgattgcaatgatgagggcattccctaccttgaagctcacgtcaattgcaaactctctgatttccccaagaatccgatccctgatcaacttagcaagttcatgccatttgaacttgatgatcatattgcccatggcgaatccaacatagagcatccagactttctgtcagcaacacgttttccactgaaggatgttatggcatatgatcgaagcactgaaatcaccaggaataaagtcacaaagaggtttttattcagtgcctctatgatagaggctctcagagcaaagtatggggaaagcgctttaggcttagaagaaaacaggaaacgcccatcacgtgttgatgctttaacggctttcatatggagtcgatatatggccaataccaataagattacaggacctcatgatgagaagttgtatattgcggtccatgctgtgaacctacgcccaaggtttgatccaccattgccacaacattcttttggaaacatttaccgtgctgccaccacaggactacagcagggtgctgagcagtcggggctccccttcaatgagaatactggtagactaaccgaactagagctagctaagttgggcttcagtagctattgcaggtttccagtgtatgataatgattttggttggggaaggcctgcgtgggtgttctcaacaccattgaccttcaagaacgtagcaactttcatggacaccaaagatagtgatggaatagaggtatacatcagtttggaggagcaactcatggctaaattcgaaaatgatgccgagttcttggcccctatgtgtctccaagtgggcgttgaagagccctttggttgttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

368

Amino Acids

41.73

Weight (kDa)

5.28

Isoelectric Point (pI)

54.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24710 FvH4_4g24720 FvH4_4g24770 FvH4_4g24771 FvH4_4g24772 FvH4_5g39380
malus_domestica MD10G1199500.v1.1 MD13G1109100.v1.1 MD13G1109200.v1.1 MD13G1114400.v1.1 MD13G1114800.v1.1 MD16G1108800.v1.1
prunus_persica Prupe.1G235900_v2.0.a1 Prupe.1G236600_v2.0.a1 Prupe.1G236700_v2.0.a1 Prupe.1G236800_v2.0.a1 Prupe.4G142000_v2.0.a1
pyrus_communis pycom10g17180 pycom13g09460 pycom13g09930 pycom13g09940
rosa_chinensis RchiOBHm_Chr3g0490281 RchiOBHm_Chr3g0490291 RchiOBHm_Chr3g0490301 RchiOBHm_Chr4g0417441 RchiOBHm_Chr4g0417461 RchiOBHm_Chr4g0431811 RchiOBHm_Chr4g0431821 RchiOBHm_Chr4g0431851
rosa_laevigata RLG00000006882 RLG00000006886 RLG00000006887 RLG00000007905 RLG00000007911 RLG00000007913 RLG00000029538
rosa_multiflora Rmu_sc0001459.1_g000008 Rmu_sc0001459.1_g000011 Rmu_sc0002489.1_g000020 Rmu_sc0002489.1_g000024 Rmu_sc0002489.1_g000027 Rmu_sc0002489.1_g000028 Rmu_sc0002489.1_g000029 Rmu_sc0004142.1_g000001 Rmu_sc0004142.1_g000024 Rmu_sc0011237.1_g000018 Rmu_sc0031439.1_g000001 Rmu_sc0031677.1_g000001 Rmu_ssc0000361.1_g000005
rosa_roxburghii Rroxscaffold_3G00262760 Rroxscaffold_4G00317310 Rroxscaffold_5G00361150 Rroxscaffold_5G00373190 Rroxscaffold_5G00373210 Rroxscaffold_5G00373220 Rroxscaffold_5G00373240
rosa_rugosa Rorug01G0111100 Rorug04G0252400 Rorug04G0252400 Rorug04G0252400 Rorug04G0252600
rosa_samantha Rh1BG102300 Rh3AG292200 Rh3AG292300 Rh4AG309100 Rh4AG309300 Rh4AG309500 Rh6BG200900
rosa_wichuraiana Rw0G002990 Rw0G003000 Rw0G019170 Rw3G025890 Rw3G025900 Rw4G017830 Rw4G026810 Rw4G026870 Rw4G026880 Rw4G026920 Rw6G017190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 870
AccI GTMKAC 2 cut(s) 832, 1002
AciI CCGC 1 cut(s) 692
AclWI GGATC 2 cut(s) 334, 719
AcoI YGGCCR 1 cut(s) 642
AcsI RAATTY 2 cut(s) 140, 1034
AcuI CTGAAG 2 cut(s) 470, 850
AcvI CACGTG 1 cut(s) 602
AfeI AGCGCT 1 cut(s) 565
AfiI CCNNNNNNNGG 1 cut(s) 233
AflIII ACRYGT 2 cut(s) 437, 601
AgsI TTSAA 7 cut(s) 11, 145, 296, 374, 817, 952, 1088
AhdI GACNNNNNGTC 1 cut(s) 427
AjiI CACGTC 1 cut(s) 304
AjnI CCWGG 1 cut(s) 488
AjuI GAANNNNNNNTTGG 1 cut(s) 1080
AluBI AGCT 6 cut(s) 82, 104, 299, 850, 854, 873
AluI AGCT 6 cut(s) 82, 104, 299, 850, 854, 873
Alw26I GTCTC 1 cut(s) 1076
AlwI GGATC 2 cut(s) 334, 719
Aor51HI AGCGCT 1 cut(s) 565
AoxI GGCC 3 cut(s) 642, 917, 1059
ApeKI GCWGC 1 cut(s) 767
ApoI RAATTY 2 cut(s) 140, 1034
AspLEI GCGC 1 cut(s) 566
AspS9I GGNCC 4 cut(s) 127, 665, 694, 1060
AsuHPI GGTGA 1 cut(s) 478
AsuII TTCGAA 1 cut(s) 1038
AsuNHI GCTAGC 1 cut(s) 850
AvaII GGWCC 3 cut(s) 127, 665, 694
BalI TGGCCA 1 cut(s) 644
BanII GRGCYC 2 cut(s) 810, 1095
BbrPI CACGTG 1 cut(s) 602
BbsI GAAGAC 1 cut(s) 59
BbvI GCAGC 1 cut(s) 754
BccI CCATC 2 cut(s) 604, 983
BceAI ACGGC 1 cut(s) 633
BcgI CGANNNNNNTGC 2 cut(s) 1028, 1062
BciT130I CCWGG 1 cut(s) 490
BclI TGATCA 3 cut(s) 94, 346, 382
BcoDI GTCTC 1 cut(s) 1076
BfaI CTAG 3 cut(s) 200, 845, 851
BfmI CTRYAG 1 cut(s) 782
BfoI RGCGCY 1 cut(s) 567
BfuAI ACCTGC 1 cut(s) 870
BisI GCNGC 1 cut(s) 768
BlpI GCTNAGC 1 cut(s) 795
BlsI GCNGC 1 cut(s) 769
Bme1390I CCNGG 1 cut(s) 490
Bme18I GGWCC 3 cut(s) 127, 665, 694
BmeRI GACNNNNNGTC 1 cut(s) 427
BmgBI CACGTC 1 cut(s) 304
BmgT120I GGNCC 4 cut(s) 127, 665, 694, 1060
BmiI GGNNCC 2 cut(s) 809, 1062
BmrFI CCNGG 1 cut(s) 490
BmrI ACTGGG 1 cut(s) 104
BmsI GCATC 3 cut(s) 424, 598, 1036
BmtI GCTAGC 1 cut(s) 854
BmuI ACTGGG 1 cut(s) 104
BpiI GAAGAC 1 cut(s) 59
Bpu1102I GCTNAGC 1 cut(s) 795
Bpu14I TTCGAA 1 cut(s) 1038
BsaAI YACGTR 1 cut(s) 602
BsaJI CCNNGG 3 cut(s) 27, 394, 716
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bsc4I CCNNNNNNNGG 1 cut(s) 233
Bse1I ACTGG 4 cut(s) 110, 260, 832, 887
Bse3DI GCAATG 2 cut(s) 277, 731
BseBI CCWGG 1 cut(s) 490
BseDI CCNNGG 3 cut(s) 27, 394, 716
BseGI GGATG 2 cut(s) 415, 460
BseLI CCNNNNNNNGG 1 cut(s) 233
BseMI GCAATG 2 cut(s) 277, 731
BseMII CTCAG 2 cut(s) 556, 786
BseNI ACTGG 4 cut(s) 110, 260, 832, 887
BseRI GAGGAG 2 cut(s) 76, 1031
BseXI GCAGC 1 cut(s) 754
BshFI GGCC 3 cut(s) 644, 919, 1061
BsiSI CCGG 1 cut(s) 234
BslFI GGGAC 1 cut(s) 181
BslI CCNNNNNNNGG 1 cut(s) 233
BsmAI GTCTC 1 cut(s) 1076
BsmFI GGGAC 1 cut(s) 181
BsmI GAATGC 1 cut(s) 282
BsnI GGCC 3 cut(s) 644, 919, 1061
Bsp119I TTCGAA 1 cut(s) 1038
Bsp1286I GDGCHC 2 cut(s) 810, 1095
Bsp143I GATC 6 cut(s) 94, 339, 346, 382, 469, 724
Bsp1720I GCTNAGC 1 cut(s) 795
Bsp19I CCATGG 1 cut(s) 394
BspACI CCGC 1 cut(s) 692
BspANI GGCC 3 cut(s) 644, 919, 1061
BspCNI CTCAG 2 cut(s) 555, 787
BspHI TCATGA 1 cut(s) 670
BspLI GGNNCC 2 cut(s) 809, 1062
BspMI ACCTGC 1 cut(s) 870
BspOI GCTAGC 1 cut(s) 854
BspPI GGATC 2 cut(s) 334, 719
BspQI GCTCTTC 1 cut(s) 1083
BspT104I TTCGAA 1 cut(s) 1038
BsrDI GCAATG 2 cut(s) 277, 731
BsrI ACTGG 4 cut(s) 110, 260, 832, 887
BssECI CCNNGG 3 cut(s) 27, 394, 716
BssMI GATC 6 cut(s) 94, 339, 346, 382, 469, 724
BssNAI GTATAC 1 cut(s) 1003
BssT1I CCWWGG 3 cut(s) 27, 394, 716
Bst1107I GTATAC 1 cut(s) 1003
Bst2UI CCWGG 1 cut(s) 490
Bst4CI ACNGT 2 cut(s) 217, 764
Bst6I CTCTTC 2 cut(s) 137, 1083
BstBAI YACGTR 1 cut(s) 602
BstBI TTCGAA 1 cut(s) 1038
BstC8I GCNNGC 2 cut(s) 852, 921
BstDEI CTNAG 5 cut(s) 353, 542, 574, 795, 855
BstDSI CCRYGG 1 cut(s) 394
BstF5I GGATG 2 cut(s) 415, 460
BstH2I RGCGCY 1 cut(s) 567
BstHHI GCGC 1 cut(s) 566
BstKTI GATC 6 cut(s) 97, 342, 349, 385, 472, 727
BstMAI GTCTC 1 cut(s) 1076
BstMBI GATC 6 cut(s) 94, 339, 346, 382, 469, 724
BstMWI GCNNNNNNNGC 6 cut(s) 545, 570, 617, 698, 805, 870
BstNI CCWGG 1 cut(s) 490
BstNSI RCATGY 1 cut(s) 263
BstSCI CCNGG 1 cut(s) 488
BstSFI CTRYAG 1 cut(s) 782
BstV1I GCAGC 1 cut(s) 754
BstV2I GAAGAC 1 cut(s) 59
BstZ17I GTATAC 1 cut(s) 1003
BsuRI GGCC 3 cut(s) 644, 919, 1061
BtgI CCRYGG 1 cut(s) 394
BtrI CACGTC 1 cut(s) 304
BtsCI GGATG 2 cut(s) 415, 460
BtsIMutI CAGTG 4 cut(s) 446, 477, 526, 894
BveI ACCTGC 1 cut(s) 870
Cac8I GCNNGC 2 cut(s) 852, 921
CciI TCATGA 1 cut(s) 670
CfoI GCGC 1 cut(s) 566
Cfr13I GGNCC 4 cut(s) 127, 665, 694, 1060
CviAII CATG 7 cut(s) 260, 364, 395, 671, 698, 970, 1027
DdeI CTNAG 5 cut(s) 353, 542, 574, 795, 855
DpnI GATC 6 cut(s) 96, 341, 348, 384, 471, 726
DpnII GATC 6 cut(s) 94, 339, 346, 382, 469, 724
DriI GACNNNNNGTC 1 cut(s) 427
EaeI YGGCCR 1 cut(s) 642
Eam1104I CTCTTC 2 cut(s) 137, 1083
Eam1105I GACNNNNNGTC 1 cut(s) 427
EarI CTCTTC 2 cut(s) 137, 1083
Eco130I CCWWGG 3 cut(s) 27, 394, 716
Eco147I AGGCCT 1 cut(s) 919
Eco24I GRGCYC 2 cut(s) 810, 1095
Eco47I GGWCC 3 cut(s) 127, 665, 694
Eco47III AGCGCT 1 cut(s) 565
Eco57I CTGAAG 2 cut(s) 470, 850
Eco72I CACGTG 1 cut(s) 602
EcoO109I RGGNCCY 1 cut(s) 665
EcoRI GAATTC 1 cut(s) 140
EcoRII CCWGG 1 cut(s) 488
EcoT14I CCWWGG 3 cut(s) 27, 394, 716
EcoT38I GRGCYC 2 cut(s) 810, 1095
ErhI CCWWGG 3 cut(s) 27, 394, 716
FaeI CATG 7 cut(s) 263, 367, 398, 674, 701, 973, 1030
FalI AAGNNNNNCTT 2 cut(s) 848, 880
FaqI GGGAC 1 cut(s) 181
FatI CATG 7 cut(s) 259, 363, 394, 670, 697, 969, 1026
FauNDI CATATG 2 cut(s) 466, 626
FbaI TGATCA 3 cut(s) 94, 346, 382
FblI GTMKAC 2 cut(s) 832, 1002
Fnu4HI GCNGC 1 cut(s) 768
FokI GGATG 2 cut(s) 402, 467
FriOI GRGCYC 2 cut(s) 810, 1095
Fsp4HI GCNGC 1 cut(s) 768
FspBI CTAG 3 cut(s) 200, 845, 851
GlaI GCGC 1 cut(s) 565
GluI GCNGC 1 cut(s) 768
HaeII RGCGCY 1 cut(s) 567
HaeIII GGCC 3 cut(s) 644, 919, 1061
HapII CCGG 1 cut(s) 234
HhaI GCGC 1 cut(s) 566
Hin1II CATG 7 cut(s) 263, 367, 398, 674, 701, 973, 1030
Hin6I GCGC 1 cut(s) 564
HinP1I GCGC 1 cut(s) 564
HinfI GANTC 4 cut(s) 240, 334, 401, 631
HpaII CCGG 1 cut(s) 234
HphI GGTGA 1 cut(s) 478
Hpy166II GTNNAC 3 cut(s) 706, 833, 1003
Hpy188I TCNGA 4 cut(s) 54, 322, 339, 545
Hpy188III TCNNGA 3 cut(s) 419, 671, 952
Hpy8I GTNNAC 3 cut(s) 706, 833, 1003
Hpy99I CGWCG 1 cut(s) 254
HpyAV CCTTC 4 cut(s) 445, 823, 909, 958
HpyCH4III ACNGT 2 cut(s) 217, 764
HpyCH4IV ACGT 4 cut(s) 303, 439, 601, 957
HpyCH4V TGCA 3 cut(s) 270, 312, 879
HpyF10VI GCNNNNNNNGC 6 cut(s) 545, 570, 617, 698, 805, 870
HpyF3I CTNAG 5 cut(s) 353, 542, 574, 795, 855
HpySE526I ACGT 4 cut(s) 303, 439, 601, 957
Hsp92II CATG 7 cut(s) 263, 367, 398, 674, 701, 973, 1030
HspAI GCGC 1 cut(s) 564
Ksp22I TGATCA 3 cut(s) 94, 346, 382
Kzo9I GATC 6 cut(s) 94, 339, 346, 382, 469, 724
LguI GCTCTTC 1 cut(s) 1083
LmnI GCTCC 3 cut(s) 109, 813, 1018
Lsp1109I GCAGC 1 cut(s) 754
LweI GCATC 3 cut(s) 424, 598, 1036
MaeI CTAG 3 cut(s) 200, 845, 851
MaeII ACGT 4 cut(s) 303, 439, 601, 957
MaeIII GTNAC 1 cut(s) 499
MalI GATC 6 cut(s) 96, 341, 348, 384, 471, 726
MboI GATC 6 cut(s) 94, 339, 346, 382, 469, 724
MboII GAAGA 5 cut(s) 39, 59, 124, 590, 1100
MfeI CAATTG 1 cut(s) 307
MhlI GDGCHC 2 cut(s) 810, 1095
MlsI TGGCCA 1 cut(s) 644
MluCI AATT 4 cut(s) 98, 140, 307, 1034
MluNI TGGCCA 1 cut(s) 644
MlyI GAGTC 1 cut(s) 640
MmeI TCCRAC 2 cut(s) 77, 429
Mox20I TGGCCA 1 cut(s) 644
MscI TGGCCA 1 cut(s) 644
MseI TTAA 3 cut(s) 212, 614, 1105
Msp20I TGGCCA 1 cut(s) 644
MspI CCGG 1 cut(s) 234
MspR9I CCNGG 1 cut(s) 490
MunI CAATTG 1 cut(s) 307
Mva1269I GAATGC 1 cut(s) 282
MvaI CCWGG 1 cut(s) 490
MwoI GCNNNNNNNGC 6 cut(s) 545, 570, 617, 698, 805, 870
NcoI CCATGG 1 cut(s) 394
NdeI CATATG 2 cut(s) 466, 626
NdeII GATC 6 cut(s) 94, 339, 346, 382, 469, 724
NheI GCTAGC 1 cut(s) 850
NlaIII CATG 7 cut(s) 263, 367, 398, 674, 701, 973, 1030
NlaIV GGNNCC 2 cut(s) 809, 1062
NmeAIII GCCGAG 1 cut(s) 1075
NmuCI GTSAC 1 cut(s) 499
NspI RCATGY 1 cut(s) 263
NspV TTCGAA 1 cut(s) 1038
PagI TCATGA 1 cut(s) 670
PceI AGGCCT 1 cut(s) 919
PciSI GCTCTTC 1 cut(s) 1083
PcsI WCGNNNNNNNCGW 1 cut(s) 598
PctI GAATGC 1 cut(s) 282
PfeI GAWTC 3 cut(s) 240, 334, 401
PkrI GCNGC 1 cut(s) 769
PleI GAGTC 1 cut(s) 639
PmaCI CACGTG 1 cut(s) 602
PmlI CACGTG 1 cut(s) 602
PpsI GAGTC 1 cut(s) 639
Ppu21I YACGTR 1 cut(s) 602
PpuMI RGGWCCY 1 cut(s) 665
Psp5II RGGWCCY 1 cut(s) 665
Psp6I CCWGG 1 cut(s) 488
PspCI CACGTG 1 cut(s) 602
PspGI CCWGG 1 cut(s) 488
PspN4I GGNNCC 2 cut(s) 809, 1062
PspPI GGNCC 4 cut(s) 127, 665, 694, 1060
PspPPI RGGWCCY 1 cut(s) 665
SapI GCTCTTC 1 cut(s) 1083
SaqAI TTAA 3 cut(s) 212, 614, 1105
SatI GCNGC 1 cut(s) 768
Sau3AI GATC 6 cut(s) 94, 339, 346, 382, 469, 724
Sau96I GGNCC 4 cut(s) 127, 665, 694, 1060
SchI GAGTC 1 cut(s) 640
ScrFI CCNGG 1 cut(s) 490
SduI GDGCHC 2 cut(s) 810, 1095
SfaNI GCATC 3 cut(s) 424, 598, 1036
SfcI CTRYAG 1 cut(s) 782
SfuI TTCGAA 1 cut(s) 1038
SinI GGWCC 3 cut(s) 127, 665, 694
Sse9I AATT 4 cut(s) 98, 140, 307, 1034
SseBI AGGCCT 1 cut(s) 919
SsiI CCGC 1 cut(s) 692
SspMI CTAG 3 cut(s) 200, 845, 851
StuI AGGCCT 1 cut(s) 919
StyD4I CCNGG 1 cut(s) 488
StyI CCWWGG 3 cut(s) 27, 394, 716
TaaI ACNGT 2 cut(s) 217, 764
TaiI ACGT 4 cut(s) 306, 442, 604, 960
TaqI TCGA 3 cut(s) 472, 634, 1038
TasI AATT 4 cut(s) 98, 140, 307, 1034
TfiI GAWTC 3 cut(s) 240, 334, 401
Tru1I TTAA 3 cut(s) 212, 614, 1105
Tru9I TTAA 3 cut(s) 212, 614, 1105
TscAI CASTG 4 cut(s) 453, 484, 526, 894
TseFI GTSAC 1 cut(s) 499
TseI GCWGC 1 cut(s) 767
Tsp45I GTSAC 1 cut(s) 499
TspDTI ATGAA 4 cut(s) 17, 352, 613, 958
TspRI CASTG 4 cut(s) 453, 484, 526, 894
VpaK11BI GGWCC 3 cut(s) 127, 665, 694
XapI RAATTY 2 cut(s) 140, 1034
XceI RCATGY 1 cut(s) 263
XmiI GTMKAC 2 cut(s) 832, 1002
XspI CTAG 3 cut(s) 200, 845, 851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.