MD13G1114800.v1.1

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000622
Physical Location & Seq
Reverse (-)
8321924 .. 8323330
1407 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1114800.v1.1.491

Sequence Viewer

Length: 1407 bp
ATGTATACCCTTATTGTTTTGTTATCAACATTAATTAGCTCCAACTTCTTGCTACTAAATAAAACATTCGAGCTGATGAAGGTTGAGGTTGAAGTGATCTCCAATGAGAATATCAAACCATCTTCTCCAACTCCTGGCCACCTTCGCCATTACCAATTTTCCTTTCTTGATCAAATGGCTCCCCAAGTCTACAACCCGCTGCTCCTCTTCTATGAATACAATGCCAATATACATCCCAACATCACTGAAATATCCAACCACCTTAAGAACTCCTTATCCGAGGCCCTATCGCTATTCTACCCGCTAGCCGGTCGAATCAAAGACAACCGCTTCATACATTGCAATGACGAGGGCATCCCCTACGTCGAAGCTCGAGTGCTGAATTGCACTCTCTCTGATGTTCTCAGCAACCCCAACCCTGGTGAACTTAACAAGTTCATGCCATTTGAACTTGATGATATTACCAATCCATTACCCTTTGGGGTCCAGCTCAACATATTTCGGTGTGGAGGGTTTGCTATTGGCCAATGCATTTCTCACAAGATTGCTGATGGGTTGTCCTATTTCACGTTCAGCAAAATTTGGGCAGCCATCGCCCGTGGGGAACAAGCCAACATAGACCCTCCACAATTTGTGTCAGCCACACTCTTCCCACCAAAGGAGTTAAATACTGGATTCGATGGAGGCGTTGGAATCACAAAAGATAGAGTGACAAAGAGGTTCGTGTTCAATGCCTCTAAAATAGAGACCCTCCAAGCAAAATATGAAAAAGATATGAAAAGCCTCCAACTAAAACACCCCACGCGTGTTGAGACCTTATCCGCTTTCATATGGAGTCGATATGTGGCGGCCACAAAGGATTGTACCGTGAATAAGTTGTACATGGTGATCCATGCAGTGAACCTACGTCCGCGGTTTAGTCCACCATTGCCTCAACATTCTTTTGGAAATCTTTTTCGTTTTTCCATGACATCACCATTGCGAATCAGTTATGGCGGGCAGGAAGAATGTGATGGGGTGGTGATTGGGGAGGTGCGAGAAGCGATAAGCAAGATTGACAATGAGTACGTGAAGCAATTACAAAGGCAGGGTGAGAAGCACTTGGGTTTGATGAAGAAAGCTGCTGATAATTTCAAGAGAGGAGACATGGTTGCGGTACTTAGCTTCAGTAGCTATTGTAGGTTTCCTCTCTATGAAAATGACTTTGGTTGGGGTAAACCTGCGTGGGTGGGATCACCGGCACTGACCTACAAGAACCTAGTGCTTTTCATGGACACCAAGGAGGGTGATGGAATAGAGGCATATGTTAGCTTGGAGGAGAGAGTCATGGCCAAATTTGAATGTGATAGCGAGTTGCTCTCTTATGTTTCTCCAGCCGGTCGGGTCCTGCTGAGCTGTGGAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

469

Amino Acids

52.76

Weight (kDa)

6.8

Isoelectric Point (pI)

44.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 29 - 452 4.2e-83 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24710 FvH4_4g24720 FvH4_4g24770 FvH4_4g24771 FvH4_4g24772 FvH4_5g39380
malus_domestica MD10G1199500.v1.1 MD13G1109100.v1.1 MD13G1109200.v1.1 MD13G1114400.v1.1 MD13G1114800.v1.1 MD16G1108800.v1.1
prunus_persica Prupe.1G235900_v2.0.a1 Prupe.1G236600_v2.0.a1 Prupe.1G236700_v2.0.a1 Prupe.1G236800_v2.0.a1 Prupe.4G142000_v2.0.a1
pyrus_communis pycom10g17180 pycom13g09460 pycom13g09930 pycom13g09940
rosa_chinensis RchiOBHm_Chr3g0490281 RchiOBHm_Chr3g0490291 RchiOBHm_Chr3g0490301 RchiOBHm_Chr4g0417441 RchiOBHm_Chr4g0417461 RchiOBHm_Chr4g0431811 RchiOBHm_Chr4g0431821 RchiOBHm_Chr4g0431851
rosa_laevigata RLG00000006882 RLG00000006886 RLG00000006887 RLG00000007905 RLG00000007911 RLG00000007913 RLG00000029538
rosa_multiflora Rmu_sc0001459.1_g000008 Rmu_sc0001459.1_g000011 Rmu_sc0002489.1_g000020 Rmu_sc0002489.1_g000024 Rmu_sc0002489.1_g000027 Rmu_sc0002489.1_g000028 Rmu_sc0002489.1_g000029 Rmu_sc0004142.1_g000001 Rmu_sc0004142.1_g000024 Rmu_sc0011237.1_g000018 Rmu_sc0031439.1_g000001 Rmu_sc0031677.1_g000001 Rmu_ssc0000361.1_g000005
rosa_roxburghii Rroxscaffold_3G00262760 Rroxscaffold_4G00317310 Rroxscaffold_5G00361150 Rroxscaffold_5G00373190 Rroxscaffold_5G00373210 Rroxscaffold_5G00373220 Rroxscaffold_5G00373240
rosa_rugosa Rorug01G0111100 Rorug04G0252400 Rorug04G0252400 Rorug04G0252400 Rorug04G0252600
rosa_samantha Rh1BG102300 Rh3AG292200 Rh3AG292300 Rh4AG309100 Rh4AG309300 Rh4AG309500 Rh6BG200900
rosa_wichuraiana Rw0G002990 Rw0G003000 Rw0G019170 Rw3G025890 Rw3G025900 Rw4G017830 Rw4G026810 Rw4G026870 Rw4G026880 Rw4G026920 Rw6G017190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1228
AccB7I CCANNNNNTGG 1 cut(s) 134
AccI GTMKAC 2 cut(s) 5, 189
AccII CGCG 2 cut(s) 805, 913
AciI CCGC 9 cut(s) 197, 302, 328, 822, 848, 911, 913, 996, 1154
AclWI GGATC 2 cut(s) 883, 1240
AcoI YGGCCR 4 cut(s) 136, 523, 849, 1329
AcsI RAATTY 2 cut(s) 579, 1334
AcuI CTGAAG 1 cut(s) 1150
AfaI GTAC 4 cut(s) 865, 881, 1067, 1158
AfiI CCNNNNNNNGG 4 cut(s) 134, 308, 419, 658
AflII CTTAAG 1 cut(s) 263
AflIII ACRYGT 1 cut(s) 803
AgsI TTSAA 5 cut(s) 92, 449, 730, 1135, 1340
AjnI CCWGG 2 cut(s) 133, 418
AjuI GAANNNNNNNTTGG 4 cut(s) 147, 179, 1188, 1220
AloI GAACNNNNNNTCC 2 cut(s) 260, 292
AluBI AGCT 9 cut(s) 39, 73, 371, 490, 1121, 1164, 1173, 1311, 1395
AluI AGCT 9 cut(s) 39, 73, 371, 490, 1121, 1164, 1173, 1311, 1395
Alw26I GTCTC 3 cut(s) 740, 806, 1137
AlwI GGATC 2 cut(s) 883, 1240
Ama87I CYCGRG 1 cut(s) 372
AoxI GGCC 5 cut(s) 136, 282, 523, 849, 1329
ApeKI GCWGC 3 cut(s) 199, 587, 1121
ApoI RAATTY 2 cut(s) 579, 1334
AseI ATTAAT 1 cut(s) 32
AspS9I GGNCC 3 cut(s) 283, 484, 1384
AsuHPI GGTGA 7 cut(s) 434, 898, 966, 1033, 1103, 1227, 1298
AsuNHI GCTAGC 1 cut(s) 304
AvaI CYCGRG 1 cut(s) 372
AvaII GGWCC 2 cut(s) 484, 1384
BalI TGGCCA 3 cut(s) 138, 525, 1331
BbvI GCAGC 3 cut(s) 186, 599, 1108
BccI CCATC 6 cut(s) 127, 545, 599, 674, 1007, 1283
BciT130I CCWGG 2 cut(s) 135, 420
BclI TGATCA 1 cut(s) 169
BcoDI GTCTC 3 cut(s) 740, 806, 1137
BfaI CTAG 2 cut(s) 305, 1259
BfrI CTTAAG 1 cut(s) 263
BfuAI ACCTGC 1 cut(s) 1228
BisI GCNGC 4 cut(s) 200, 588, 849, 1122
BlpI GCTNAGC 1 cut(s) 1391
BlsI GCNGC 4 cut(s) 201, 589, 850, 1123
Bme1390I CCNGG 2 cut(s) 135, 420
Bme18I GGWCC 2 cut(s) 484, 1384
BmeT110I CYCGRG 1 cut(s) 372
BmgT120I GGNCC 3 cut(s) 283, 484, 1384
BmiI GGNNCC 3 cut(s) 180, 485, 1385
BmrFI CCNGG 2 cut(s) 135, 420
BmsI GCATC 1 cut(s) 363
BmtI GCTAGC 1 cut(s) 308
BplI GAGNNNNNCTC 2 cut(s) 1343, 1375
BpmI CTGGAG 1 cut(s) 1356
Bpu1102I GCTNAGC 1 cut(s) 1391
BsaAI YACGTR 1 cut(s) 1069
BsaI GGTCTC 2 cut(s) 740, 806
BsaJI CCNNGG 5 cut(s) 279, 418, 598, 911, 1278
BsaXI ACNNNNNCTCC 2 cut(s) 1134, 1164
Bsc4I CCNNNNNNNGG 4 cut(s) 134, 308, 419, 658
Bse118I RCCGGY 3 cut(s) 308, 1237, 1376
Bse1I ACTGG 1 cut(s) 676
Bse3DI GCAATG 4 cut(s) 337, 349, 926, 977
BseBI CCWGG 2 cut(s) 135, 420
BseDI CCNNGG 5 cut(s) 279, 418, 598, 911, 1278
BseGI GGATG 2 cut(s) 232, 354
BseLI CCNNNNNNNGG 4 cut(s) 134, 308, 419, 658
BseMI GCAATG 4 cut(s) 337, 349, 926, 977
BseMII CTCAG 2 cut(s) 418, 1382
BseNI ACTGG 1 cut(s) 676
BseRI GAGGAG 3 cut(s) 194, 1155, 1331
BseXI GCAGC 3 cut(s) 186, 599, 1108
Bsh1236I CGCG 2 cut(s) 805, 913
Bsh1285I CGRYCG 2 cut(s) 313, 1381
BshFI GGCC 5 cut(s) 138, 284, 525, 851, 1331
BsiEI CGRYCG 2 cut(s) 313, 1381
BsiHKCI CYCGRG 1 cut(s) 372
BsiSI CCGG 3 cut(s) 309, 1238, 1377
BslI CCNNNNNNNGG 4 cut(s) 134, 308, 419, 658
BsmAI GTCTC 3 cut(s) 740, 806, 1137
BsnI GGCC 5 cut(s) 138, 284, 525, 851, 1331
Bso31I GGTCTC 2 cut(s) 740, 806
BsoBI CYCGRG 1 cut(s) 372
Bsp1407I TGTACA 1 cut(s) 879
Bsp143I GATC 4 cut(s) 96, 169, 888, 1232
Bsp1720I GCTNAGC 1 cut(s) 1391
BspACI CCGC 9 cut(s) 197, 302, 328, 822, 848, 911, 913, 996, 1154
BspANI GGCC 5 cut(s) 138, 284, 525, 851, 1331
BspCNI CTCAG 2 cut(s) 417, 1383
BspFNI CGCG 2 cut(s) 805, 913
BspLI GGNNCC 3 cut(s) 180, 485, 1385
BspMI ACCTGC 1 cut(s) 1228
BspOI GCTAGC 1 cut(s) 308
BspPI GGATC 2 cut(s) 883, 1240
BspTI CTTAAG 1 cut(s) 263
BspTNI GGTCTC 2 cut(s) 740, 806
BsrDI GCAATG 4 cut(s) 337, 349, 926, 977
BsrFI RCCGGY 3 cut(s) 308, 1237, 1376
BsrGI TGTACA 1 cut(s) 879
BsrI ACTGG 1 cut(s) 676
BssAI RCCGGY 3 cut(s) 308, 1237, 1376
BssECI CCNNGG 5 cut(s) 279, 418, 598, 911, 1278
BssMI GATC 4 cut(s) 96, 169, 888, 1232
BssNAI GTATAC 1 cut(s) 6
BssT1I CCWWGG 1 cut(s) 1278
Bst1107I GTATAC 1 cut(s) 6
Bst2UI CCWGG 2 cut(s) 135, 420
Bst4CI ACNGT 1 cut(s) 868
Bst6I CTCTTC 2 cut(s) 212, 653
BstAFI CTTAAG 1 cut(s) 263
BstAUI TGTACA 1 cut(s) 879
BstBAI YACGTR 1 cut(s) 1069
BstC8I GCNNGC 2 cut(s) 306, 998
BstDEI CTNAG 3 cut(s) 404, 1160, 1391
BstDSI CCRYGG 2 cut(s) 598, 911
BstF5I GGATG 2 cut(s) 232, 354
BstFNI CGCG 2 cut(s) 805, 913
BstKTI GATC 4 cut(s) 99, 172, 891, 1235
BstMAI GTCTC 3 cut(s) 740, 806, 1137
BstMBI GATC 4 cut(s) 96, 169, 888, 1232
BstMCI CGRYCG 2 cut(s) 313, 1381
BstMWI GCNNNNNNNGC 3 cut(s) 144, 593, 1170
BstNI CCWGG 2 cut(s) 135, 420
BstSCI CCNGG 2 cut(s) 133, 418
BstUI CGCG 2 cut(s) 805, 913
BstV1I GCAGC 3 cut(s) 186, 599, 1108
BstZ17I GTATAC 1 cut(s) 6
BsuRI GGCC 5 cut(s) 138, 284, 525, 851, 1331
BtgI CCRYGG 2 cut(s) 598, 911
BtgZI GCGATG 1 cut(s) 577
BtsCI GGATG 2 cut(s) 232, 354
BtsI GCAGTG 1 cut(s) 903
BtsIMutI CAGTG 3 cut(s) 243, 903, 1241
BveI ACCTGC 1 cut(s) 1228
Cac8I GCNNGC 2 cut(s) 306, 998
Cfr10I RCCGGY 3 cut(s) 308, 1237, 1376
Cfr13I GGNCC 3 cut(s) 283, 484, 1384
Cfr42I CCGCGG 1 cut(s) 914
Csp6I GTAC 4 cut(s) 864, 880, 1066, 1157
CspCI CAANNNNNGTGG 2 cut(s) 790, 825
CviAII CATG 7 cut(s) 439, 883, 893, 967, 1147, 1270, 1327
CviQI GTAC 4 cut(s) 864, 880, 1066, 1157
DdeI CTNAG 3 cut(s) 404, 1160, 1391
DpnI GATC 4 cut(s) 98, 171, 890, 1234
DpnII GATC 4 cut(s) 96, 169, 888, 1232
EaeI YGGCCR 4 cut(s) 136, 523, 849, 1329
Eam1104I CTCTTC 2 cut(s) 212, 653
EarI CTCTTC 2 cut(s) 212, 653
Eco130I CCWWGG 1 cut(s) 1278
Eco31I GGTCTC 2 cut(s) 740, 806
Eco47I GGWCC 2 cut(s) 484, 1384
Eco57I CTGAAG 1 cut(s) 1150
Eco88I CYCGRG 1 cut(s) 372
EcoO109I RGGNCCY 2 cut(s) 283, 1384
EcoRII CCWGG 2 cut(s) 133, 418
EcoT14I CCWWGG 1 cut(s) 1278
EcoT22I ATGCAT 1 cut(s) 533
ErhI CCWWGG 1 cut(s) 1278
FaeI CATG 7 cut(s) 442, 886, 896, 970, 1150, 1273, 1330
FalI AAGNNNNNCTT 2 cut(s) 257, 289
FatI CATG 7 cut(s) 438, 882, 892, 966, 1146, 1269, 1326
FauI CCCGC 3 cut(s) 204, 309, 989
FauNDI CATATG 2 cut(s) 830, 1303
FbaI TGATCA 1 cut(s) 169
FblI GTMKAC 2 cut(s) 5, 189
Fnu4HI GCNGC 4 cut(s) 200, 588, 849, 1122
FokI GGATG 2 cut(s) 219, 341
Fsp4HI GCNGC 4 cut(s) 200, 588, 849, 1122
FspBI CTAG 2 cut(s) 305, 1259
GluI GCNGC 4 cut(s) 200, 588, 849, 1122
GsuI CTGGAG 1 cut(s) 1356
HaeIII GGCC 5 cut(s) 138, 284, 525, 851, 1331
HapII CCGG 3 cut(s) 309, 1238, 1377
Hin1II CATG 7 cut(s) 442, 886, 896, 970, 1150, 1273, 1330
HinfI GANTC 6 cut(s) 315, 675, 693, 835, 984, 1323
HpaII CCGG 3 cut(s) 309, 1238, 1377
HphI GGTGA 7 cut(s) 434, 898, 966, 1033, 1103, 1227, 1298
Hpy166II GTNNAC 6 cut(s) 6, 190, 425, 901, 923, 1217
Hpy188I TCNGA 2 cut(s) 280, 397
Hpy188III TCNNGA 2 cut(s) 167, 1135
Hpy8I GTNNAC 6 cut(s) 6, 190, 425, 901, 923, 1217
Hpy99I CGWCG 1 cut(s) 368
HpyAV CCTTC 2 cut(s) 73, 152
HpyCH4III ACNGT 1 cut(s) 868
HpyCH4IV ACGT 4 cut(s) 363, 569, 907, 1068
HpyCH4V TGCA 4 cut(s) 342, 387, 531, 896
HpyF10VI GCNNNNNNNGC 3 cut(s) 144, 593, 1170
HpyF3I CTNAG 3 cut(s) 404, 1160, 1391
HpySE526I ACGT 4 cut(s) 363, 569, 907, 1068
Hsp92II CATG 7 cut(s) 442, 886, 896, 970, 1150, 1273, 1330
Ksp22I TGATCA 1 cut(s) 169
KspI CCGCGG 1 cut(s) 914
Kzo9I GATC 4 cut(s) 96, 169, 888, 1232
LmnI GCTCC 3 cut(s) 44, 184, 207
Lsp1109I GCAGC 3 cut(s) 186, 599, 1108
LweI GCATC 1 cut(s) 363
MaeI CTAG 2 cut(s) 305, 1259
MaeII ACGT 4 cut(s) 363, 569, 907, 1068
MaeIII GTNAC 1 cut(s) 709
MalI GATC 4 cut(s) 98, 171, 890, 1234
MboI GATC 4 cut(s) 96, 169, 888, 1232
MboII GAAGA 5 cut(s) 114, 199, 640, 1016, 1126
MlsI TGGCCA 3 cut(s) 138, 525, 1331
MluCI AATT 9 cut(s) 33, 155, 382, 579, 629, 1076, 1129, 1334, 1402
MluI ACGCGT 1 cut(s) 803
MluNI TGGCCA 3 cut(s) 138, 525, 1331
MlyI GAGTC 2 cut(s) 844, 1332
MmeI TCCRAC 5 cut(s) 66, 152, 279, 670, 811
Mox20I TGGCCA 3 cut(s) 138, 525, 1331
Mph1103I ATGCAT 1 cut(s) 533
MscI TGGCCA 3 cut(s) 138, 525, 1331
MseI TTAA 5 cut(s) 32, 264, 429, 665, 1405
MslI CAYNNNNRTG 1 cut(s) 342
Msp20I TGGCCA 3 cut(s) 138, 525, 1331
MspA1I CMGCKG 2 cut(s) 199, 913
MspCI CTTAAG 1 cut(s) 263
MspI CCGG 3 cut(s) 309, 1238, 1377
MspR9I CCNGG 2 cut(s) 135, 420
MvaI CCWGG 2 cut(s) 135, 420
MvnI CGCG 2 cut(s) 805, 913
MwoI GCNNNNNNNGC 3 cut(s) 144, 593, 1170
NdeI CATATG 2 cut(s) 830, 1303
NdeII GATC 4 cut(s) 96, 169, 888, 1232
NheI GCTAGC 1 cut(s) 304
NlaIII CATG 7 cut(s) 442, 886, 896, 970, 1150, 1273, 1330
NlaIV GGNNCC 3 cut(s) 180, 485, 1385
NmuCI GTSAC 1 cut(s) 709
NsiI ATGCAT 1 cut(s) 533
PaeR7I CTCGAG 1 cut(s) 372
PcsI WCGNNNNNNNCGW 1 cut(s) 684
PfeI GAWTC 4 cut(s) 315, 675, 693, 984
PflMI CCANNNNNTGG 1 cut(s) 134
PkrI GCNGC 4 cut(s) 201, 589, 850, 1123
PleI GAGTC 2 cut(s) 843, 1331
PpsI GAGTC 2 cut(s) 843, 1331
Ppu21I YACGTR 1 cut(s) 1069
PpuMI RGGWCCY 1 cut(s) 1384
PshBI ATTAAT 1 cut(s) 32
Psp5II RGGWCCY 1 cut(s) 1384
Psp6I CCWGG 2 cut(s) 133, 418
PspGI CCWGG 2 cut(s) 133, 418
PspN4I GGNNCC 3 cut(s) 180, 485, 1385
PspPI GGNCC 3 cut(s) 283, 484, 1384
PspPPI RGGWCCY 1 cut(s) 1384
PspXI VCTCGAGB 1 cut(s) 372
RsaI GTAC 4 cut(s) 865, 881, 1067, 1158
RsaNI GTAC 4 cut(s) 864, 880, 1066, 1157
RseI CAYNNNNRTG 1 cut(s) 342
SacII CCGCGG 1 cut(s) 914
SaqAI TTAA 5 cut(s) 32, 264, 429, 665, 1405
SatI GCNGC 4 cut(s) 200, 588, 849, 1122
Sau3AI GATC 4 cut(s) 96, 169, 888, 1232
Sau96I GGNCC 3 cut(s) 283, 484, 1384
SchI GAGTC 2 cut(s) 844, 1332
ScrFI CCNGG 2 cut(s) 135, 420
SfaNI GCATC 1 cut(s) 363
Sfr274I CTCGAG 1 cut(s) 372
Sfr303I CCGCGG 1 cut(s) 914
SgrBI CCGCGG 1 cut(s) 914
SinI GGWCC 2 cut(s) 484, 1384
SlaI CTCGAG 1 cut(s) 372
SmiMI CAYNNNNRTG 1 cut(s) 342
SmlI CTYRAG 2 cut(s) 263, 372
SmoI CTYRAG 2 cut(s) 263, 372
Sse9I AATT 9 cut(s) 33, 155, 382, 579, 629, 1076, 1129, 1334, 1402
SsiI CCGC 9 cut(s) 197, 302, 328, 822, 848, 911, 913, 996, 1154
SspMI CTAG 2 cut(s) 305, 1259
StyD4I CCNGG 2 cut(s) 133, 418
StyI CCWWGG 1 cut(s) 1278
TaaI ACNGT 1 cut(s) 868
TaiI ACGT 4 cut(s) 366, 572, 910, 1071
TaqI TCGA 6 cut(s) 69, 313, 366, 373, 678, 838
TasI AATT 9 cut(s) 33, 155, 382, 579, 629, 1076, 1129, 1334, 1402
TatI WGTACW 1 cut(s) 879
TauI GCSGC 1 cut(s) 851
TfiI GAWTC 4 cut(s) 315, 675, 693, 984
Tru1I TTAA 5 cut(s) 32, 264, 429, 665, 1405
Tru9I TTAA 5 cut(s) 32, 264, 429, 665, 1405
TscAI CASTG 3 cut(s) 250, 903, 1248
TseFI GTSAC 1 cut(s) 709
TseI GCWGC 3 cut(s) 199, 587, 1121
Tsp45I GTSAC 1 cut(s) 709
TspRI CASTG 3 cut(s) 250, 903, 1248
Van91I CCANNNNNTGG 1 cut(s) 134
Vha464I CTTAAG 1 cut(s) 263
VpaK11BI GGWCC 2 cut(s) 484, 1384
VspI ATTAAT 1 cut(s) 32
XapI RAATTY 2 cut(s) 579, 1334
XhoI CTCGAG 1 cut(s) 372
XmiI GTMKAC 2 cut(s) 5, 189
XspI CTAG 2 cut(s) 305, 1259
Zsp2I ATGCAT 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.