Rw4G026870

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000622
Physical Location & Seq
Reverse (-)
53135075 .. 53136407
1333 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G026870.1

Sequence Viewer

Length: 1257 bp
ATGAAGGTTGAAGTAGAAGTAATCTCCAAGAAGATTATCACCCCATCTTCTCCAACCCCTGCTCAACTTCGCCATTACCAGCTCTCCTCCTTTGATCAATTAGCTCCCCCAGTCTATAACCCTTTGGTCCTCTTCTACGAAATCAATGACAAGACAGTGCCCAACATTACAGAAATATCCAGCCACCTCAAAAAGTCCCTAGCCGAAGTCTTAACCGTGTTCTACCCATTAGCCGGACGAATCAAACACGACGACTGGCATGTTGATTGCAATGATGAGGGCATTCCCTACCTTGAAGCTCACGTCAATTGCAAACTCTCTGACTTCCTCAAGAATCCGATCCCTGGTCAACTTAGCAAGTTCATGCCATTTGAACTTGATGATCATATTGGATTTGCAATTGGTCAATGCATCTCTCACAGGCTTGCCGATGGGTCATCTTATTTCATGTTCAGCAAAACTTGGGCGGCCATAGCCCATGGCGAATCCAACATAGAGCATCCAGACTTTCTGTCAGCAACACGTTTTCCGCTGAAGGATGTTATGACATATGATCCAAGCACTGAAATCTCCAGGAATAAAGTCACAAAGAGGTTTTTATTCAGTGCCTCTATGATAGAGGCTCTCAGAGCAAAAAAACCAGAAACATCACGTGTTGATGCTTTAACGGCTTTCATATGGAGTCGATATATGGCCAATACCAATAGGATTACAGGACCTCATGATGAGAAGTTGTATAGGATCGCCCATGCTGTGAACCTACGCCCAAGGTTTGATCCACCATTGCCACAACATTCTTTTGGAAACATTTACCGTGGTGCCACGTCAGGTCCCTTGCTAAGTACTGGGGATGCATGCAGTGCATATGGTCGGGCTATGAGGGAAGTACGAGAAGAAATAAACAAGATTGACAATGACTGCGTCAAAGGACTACAGCAGGGTGCTGAGCAGTCTGGGCTCCCCTTCAATGAGAATACTGGTAGGCTAACCGAACTAGAACTGGTTACGTTGGGCTTCAGTAGCTATTGCAGGTTTCCAGTGTATGATAATGATTTCGGTTGGGGAAGGCCTGCGTGGGTGTTCTCAACACCATTAACCTTCAAGAACGTAGCAACTTTCATGGACACCAAAGAGGGTGATGGAATAGAGGTATACATCAGTTTGGAGGAGCAACTCATGGCTAAATTTGAAACTGATGCTGAGTTCTTGGCCCCTATGCGTCTCCAAGTGGGCGCTGAAGAGCCCTTTGGTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

418

Amino Acids

47.13

Weight (kDa)

5.69

Isoelectric Point (pI)

42.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 4 - 402 2.8e-68 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24710 FvH4_4g24720 FvH4_4g24770 FvH4_4g24771 FvH4_4g24772 FvH4_5g39380
malus_domestica MD10G1199500.v1.1 MD13G1109100.v1.1 MD13G1109200.v1.1 MD13G1114400.v1.1 MD13G1114800.v1.1 MD16G1108800.v1.1
prunus_persica Prupe.1G235900_v2.0.a1 Prupe.1G236600_v2.0.a1 Prupe.1G236700_v2.0.a1 Prupe.1G236800_v2.0.a1 Prupe.4G142000_v2.0.a1
pyrus_communis pycom10g17180 pycom13g09460 pycom13g09930 pycom13g09940
rosa_chinensis RchiOBHm_Chr3g0490281 RchiOBHm_Chr3g0490291 RchiOBHm_Chr3g0490301 RchiOBHm_Chr4g0417441 RchiOBHm_Chr4g0417461 RchiOBHm_Chr4g0431811 RchiOBHm_Chr4g0431821 RchiOBHm_Chr4g0431851
rosa_laevigata RLG00000006882 RLG00000006886 RLG00000006887 RLG00000007905 RLG00000007911 RLG00000007913 RLG00000029538
rosa_multiflora Rmu_sc0001459.1_g000008 Rmu_sc0001459.1_g000011 Rmu_sc0002489.1_g000020 Rmu_sc0002489.1_g000024 Rmu_sc0002489.1_g000027 Rmu_sc0002489.1_g000028 Rmu_sc0002489.1_g000029 Rmu_sc0004142.1_g000001 Rmu_sc0004142.1_g000024 Rmu_sc0011237.1_g000018 Rmu_sc0031439.1_g000001 Rmu_sc0031677.1_g000001 Rmu_ssc0000361.1_g000005
rosa_roxburghii Rroxscaffold_3G00262760 Rroxscaffold_4G00317310 Rroxscaffold_5G00361150 Rroxscaffold_5G00373190 Rroxscaffold_5G00373210 Rroxscaffold_5G00373220 Rroxscaffold_5G00373240
rosa_rugosa Rorug01G0111100 Rorug04G0252400 Rorug04G0252400 Rorug04G0252400 Rorug04G0252600
rosa_samantha Rh1BG102300 Rh3AG292200 Rh3AG292300 Rh4AG309100 Rh4AG309300 Rh4AG309500 Rh6BG200900
rosa_wichuraiana Rw0G002990 Rw0G003000 Rw0G019170 Rw3G025890 Rw3G025900 Rw4G017830 Rw4G026810 Rw4G026870 Rw4G026880 Rw4G026920 Rw6G017190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1020
AccB1I GGYRCC 1 cut(s) 818
AccI GTMKAC 1 cut(s) 1152
AciI CCGC 2 cut(s) 467, 530
AclWI GGATC 4 cut(s) 334, 548, 749, 770
AcoI YGGCCR 2 cut(s) 468, 693
AcsI RAATTY 1 cut(s) 1184
AcuI CTGAAG 3 cut(s) 554, 1000, 1257
AcvI CACGTG 1 cut(s) 653
AfaI GTAC 2 cut(s) 844, 888
AfiI CCNNNNNNNGG 2 cut(s) 233, 344
AflIII ACRYGT 2 cut(s) 521, 652
AgsI TTSAA 6 cut(s) 11, 296, 374, 967, 1102, 1190
AhdI GACNNNNNGTC 1 cut(s) 511
AjiI CACGTC 2 cut(s) 304, 825
AjnI CCWGG 2 cut(s) 343, 572
AjuI GAANNNNNNNTTGG 1 cut(s) 1230
AluBI AGCT 4 cut(s) 82, 104, 299, 1023
AluI AGCT 4 cut(s) 82, 104, 299, 1023
Alw26I GTCTC 1 cut(s) 1226
AlwI GGATC 4 cut(s) 334, 548, 749, 770
AoxI GGCC 4 cut(s) 468, 693, 1067, 1209
ApoI RAATTY 1 cut(s) 1184
AspLEI GCGC 1 cut(s) 1235
AspS9I GGNCC 4 cut(s) 127, 716, 830, 1210
AsuHPI GGTGA 2 cut(s) 31, 1148
AvaII GGWCC 3 cut(s) 127, 716, 830
BaeGI GKGCMC 1 cut(s) 162
BalI TGGCCA 1 cut(s) 695
BanI GGYRCC 1 cut(s) 818
BanII GRGCYC 2 cut(s) 960, 1245
BbrPI CACGTG 1 cut(s) 653
BccI CCATC 3 cut(s) 52, 425, 1133
BceAI ACGGC 1 cut(s) 684
BciT130I CCWGG 2 cut(s) 345, 574
BclI TGATCA 2 cut(s) 94, 382
BcoDI GTCTC 1 cut(s) 1226
BfaI CTAG 2 cut(s) 200, 995
BfmI CTRYAG 1 cut(s) 932
BfoI RGCGCY 1 cut(s) 1236
BfuAI ACCTGC 1 cut(s) 1020
BisI GCNGC 1 cut(s) 468
BlpI GCTNAGC 1 cut(s) 945
BlsI GCNGC 1 cut(s) 469
BmcAI AGTACT 1 cut(s) 844
Bme1390I CCNGG 2 cut(s) 345, 574
Bme18I GGWCC 3 cut(s) 127, 716, 830
BmeRI GACNNNNNGTC 1 cut(s) 511
BmgBI CACGTC 2 cut(s) 304, 825
BmgT120I GGNCC 4 cut(s) 127, 716, 830, 1210
BmiI GGNNCC 4 cut(s) 820, 832, 959, 1212
BmrFI CCNGG 2 cut(s) 345, 574
BmrI ACTGGG 2 cut(s) 104, 855
BmsI GCATC 5 cut(s) 420, 508, 649, 841, 1186
BmuI ACTGGG 2 cut(s) 104, 855
BpmI CTGGAG 1 cut(s) 556
Bpu1102I GCTNAGC 1 cut(s) 945
BpuEI CTTGAG 1 cut(s) 314
BsaAI YACGTR 1 cut(s) 653
BsaJI CCNNGG 4 cut(s) 343, 478, 767, 814
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 344
Bse1I ACTGG 6 cut(s) 110, 260, 850, 982, 1005, 1037
Bse3DI GCAATG 2 cut(s) 277, 782
BseBI CCWGG 2 cut(s) 345, 574
BseDI CCNNGG 4 cut(s) 343, 478, 767, 814
BseGI GGATG 3 cut(s) 499, 544, 856
BseLI CCNNNNNNNGG 2 cut(s) 233, 344
BseMI GCAATG 2 cut(s) 277, 782
BseMII CTCAG 3 cut(s) 640, 936, 1191
BseNI ACTGG 6 cut(s) 110, 260, 850, 982, 1005, 1037
BseRI GAGGAG 2 cut(s) 76, 1181
BseSI GKGCMC 1 cut(s) 162
BshFI GGCC 4 cut(s) 470, 695, 1069, 1211
BshNI GGYRCC 1 cut(s) 818
BsiSI CCGG 1 cut(s) 234
BslFI GGGAC 2 cut(s) 181, 816
BslI CCNNNNNNNGG 2 cut(s) 233, 344
BsmAI GTCTC 1 cut(s) 1226
BsmBI CGTCTC 1 cut(s) 1226
BsmFI GGGAC 2 cut(s) 181, 816
BsmI GAATGC 1 cut(s) 282
BsnI GGCC 4 cut(s) 470, 695, 1069, 1211
Bsp1286I GDGCHC 3 cut(s) 162, 960, 1245
Bsp143I GATC 6 cut(s) 94, 339, 382, 553, 741, 775
Bsp1720I GCTNAGC 1 cut(s) 945
Bsp19I CCATGG 1 cut(s) 478
BspACI CCGC 2 cut(s) 467, 530
BspANI GGCC 4 cut(s) 470, 695, 1069, 1211
BspCNI CTCAG 3 cut(s) 639, 937, 1192
BspHI TCATGA 1 cut(s) 721
BspLI GGNNCC 4 cut(s) 820, 832, 959, 1212
BspMI ACCTGC 1 cut(s) 1020
BspPI GGATC 4 cut(s) 334, 548, 749, 770
BspQI GCTCTTC 1 cut(s) 1233
BspT107I GGYRCC 1 cut(s) 818
BsrDI GCAATG 2 cut(s) 277, 782
BsrI ACTGG 6 cut(s) 110, 260, 850, 982, 1005, 1037
BssECI CCNNGG 4 cut(s) 343, 478, 767, 814
BssMI GATC 6 cut(s) 94, 339, 382, 553, 741, 775
BssNAI GTATAC 1 cut(s) 1153
BssT1I CCWWGG 2 cut(s) 478, 767
Bst1107I GTATAC 1 cut(s) 1153
Bst2UI CCWGG 2 cut(s) 345, 574
Bst4CI ACNGT 3 cut(s) 157, 217, 815
Bst6I CTCTTC 2 cut(s) 137, 1233
BstAPI GCANNNNNTGC 1 cut(s) 860
BstBAI YACGTR 1 cut(s) 653
BstC8I GCNNGC 3 cut(s) 426, 856, 1071
BstDEI CTNAG 5 cut(s) 353, 626, 839, 945, 1200
BstDSI CCRYGG 2 cut(s) 478, 814
BstF5I GGATG 3 cut(s) 499, 544, 856
BstH2I RGCGCY 1 cut(s) 1236
BstHHI GCGC 1 cut(s) 1235
BstKTI GATC 6 cut(s) 97, 342, 385, 556, 744, 778
BstMAI GTCTC 1 cut(s) 1226
BstMBI GATC 6 cut(s) 94, 339, 382, 553, 741, 775
BstMWI GCNNNNNNNGC 6 cut(s) 473, 629, 668, 860, 955, 1020
BstNI CCWGG 2 cut(s) 345, 574
BstNSI RCATGY 2 cut(s) 263, 858
BstSCI CCNGG 2 cut(s) 343, 572
BstSFI CTRYAG 1 cut(s) 932
BstSLI GKGCMC 1 cut(s) 162
BstZ17I GTATAC 1 cut(s) 1153
BsuRI GGCC 4 cut(s) 470, 695, 1069, 1211
BtgI CCRYGG 2 cut(s) 478, 814
BtrI CACGTC 2 cut(s) 304, 825
BtsCI GGATG 3 cut(s) 499, 544, 856
BtsI GCAGTG 1 cut(s) 865
BtsIMutI CAGTG 5 cut(s) 162, 561, 610, 865, 1044
BveI ACCTGC 1 cut(s) 1020
Cac8I GCNNGC 3 cut(s) 426, 856, 1071
CciI TCATGA 1 cut(s) 721
CfoI GCGC 1 cut(s) 1235
Cfr13I GGNCC 4 cut(s) 127, 716, 830, 1210
CseI GACGC 2 cut(s) 910, 1208
Csp6I GTAC 2 cut(s) 843, 887
CviAII CATG 9 cut(s) 260, 364, 448, 479, 722, 749, 855, 1120, 1177
CviQI GTAC 2 cut(s) 843, 887
DdeI CTNAG 5 cut(s) 353, 626, 839, 945, 1200
DpnI GATC 6 cut(s) 96, 341, 384, 555, 743, 777
DpnII GATC 6 cut(s) 94, 339, 382, 553, 741, 775
DriI GACNNNNNGTC 1 cut(s) 511
EaeI YGGCCR 2 cut(s) 468, 693
Eam1104I CTCTTC 2 cut(s) 137, 1233
Eam1105I GACNNNNNGTC 1 cut(s) 511
EarI CTCTTC 2 cut(s) 137, 1233
Eco130I CCWWGG 2 cut(s) 478, 767
Eco147I AGGCCT 1 cut(s) 1069
Eco24I GRGCYC 2 cut(s) 960, 1245
Eco47I GGWCC 3 cut(s) 127, 716, 830
Eco57I CTGAAG 3 cut(s) 554, 1000, 1257
Eco72I CACGTG 1 cut(s) 653
EcoO109I RGGNCCY 2 cut(s) 716, 830
EcoRII CCWGG 2 cut(s) 343, 572
EcoT14I CCWWGG 2 cut(s) 478, 767
EcoT22I ATGCAT 2 cut(s) 413, 856
EcoT38I GRGCYC 2 cut(s) 960, 1245
ErhI CCWWGG 2 cut(s) 478, 767
Esp3I CGTCTC 1 cut(s) 1226
FaeI CATG 9 cut(s) 263, 367, 451, 482, 725, 752, 858, 1123, 1180
FaqI GGGAC 2 cut(s) 181, 816
FatI CATG 9 cut(s) 259, 363, 447, 478, 721, 748, 854, 1119, 1176
FauNDI CATATG 3 cut(s) 550, 677, 865
FbaI TGATCA 2 cut(s) 94, 382
FblI GTMKAC 1 cut(s) 1152
Fnu4HI GCNGC 1 cut(s) 468
FokI GGATG 3 cut(s) 486, 551, 863
FriOI GRGCYC 2 cut(s) 960, 1245
Fsp4HI GCNGC 1 cut(s) 468
FspBI CTAG 2 cut(s) 200, 995
GlaI GCGC 1 cut(s) 1234
GluI GCNGC 1 cut(s) 468
GsuI CTGGAG 1 cut(s) 556
HaeII RGCGCY 1 cut(s) 1236
HaeIII GGCC 4 cut(s) 470, 695, 1069, 1211
HapII CCGG 1 cut(s) 234
HgaI GACGC 2 cut(s) 910, 1208
HhaI GCGC 1 cut(s) 1235
Hin1II CATG 9 cut(s) 263, 367, 451, 482, 725, 752, 858, 1123, 1180
Hin6I GCGC 1 cut(s) 1233
HinP1I GCGC 1 cut(s) 1233
HincII GTYRAC 1 cut(s) 350
HindII GTYRAC 1 cut(s) 350
HinfI GANTC 4 cut(s) 240, 334, 485, 682
HpaII CCGG 1 cut(s) 234
HphI GGTGA 2 cut(s) 31, 1148
Hpy166II GTNNAC 3 cut(s) 350, 757, 1153
Hpy188I TCNGA 3 cut(s) 322, 339, 629
Hpy188III TCNNGA 4 cut(s) 331, 503, 722, 1102
Hpy8I GTNNAC 3 cut(s) 350, 757, 1153
Hpy99I CGWCG 1 cut(s) 254
HpyAV CCTTC 4 cut(s) 529, 973, 1059, 1108
HpyCH4III ACNGT 3 cut(s) 157, 217, 815
HpyCH4IV ACGT 6 cut(s) 303, 523, 652, 824, 1007, 1107
HpyCH4V TGCA 8 cut(s) 270, 312, 398, 411, 854, 858, 863, 1029
HpyF10VI GCNNNNNNNGC 6 cut(s) 473, 629, 668, 860, 955, 1020
HpyF3I CTNAG 5 cut(s) 353, 626, 839, 945, 1200
HpySE526I ACGT 6 cut(s) 303, 523, 652, 824, 1007, 1107
Hsp92II CATG 9 cut(s) 263, 367, 451, 482, 725, 752, 858, 1123, 1180
HspAI GCGC 1 cut(s) 1233
Ksp22I TGATCA 2 cut(s) 94, 382
Kzo9I GATC 6 cut(s) 94, 339, 382, 553, 741, 775
LguI GCTCTTC 1 cut(s) 1233
LmnI GCTCC 3 cut(s) 109, 963, 1168
LweI GCATC 5 cut(s) 420, 508, 649, 841, 1186
MaeI CTAG 2 cut(s) 200, 995
MaeII ACGT 6 cut(s) 303, 523, 652, 824, 1007, 1107
MaeIII GTNAC 2 cut(s) 583, 1003
MalI GATC 6 cut(s) 96, 341, 384, 555, 743, 777
MboI GATC 6 cut(s) 94, 339, 382, 553, 741, 775
MboII GAAGA 5 cut(s) 39, 43, 124, 905, 1250
MfeI CAATTG 2 cut(s) 307, 399
MhlI GDGCHC 3 cut(s) 162, 960, 1245
MlsI TGGCCA 1 cut(s) 695
MluCI AATT 4 cut(s) 98, 307, 399, 1184
MluNI TGGCCA 1 cut(s) 695
MlyI GAGTC 1 cut(s) 691
MmeI TCCRAC 2 cut(s) 77, 513
Mox20I TGGCCA 1 cut(s) 695
Mph1103I ATGCAT 2 cut(s) 413, 856
MscI TGGCCA 1 cut(s) 695
MseI TTAA 4 cut(s) 212, 665, 1094, 1255
Msp20I TGGCCA 1 cut(s) 695
MspA1I CMGCKG 1 cut(s) 532
MspI CCGG 1 cut(s) 234
MspR9I CCNGG 2 cut(s) 345, 574
MunI CAATTG 2 cut(s) 307, 399
Mva1269I GAATGC 1 cut(s) 282
MvaI CCWGG 2 cut(s) 345, 574
MwoI GCNNNNNNNGC 6 cut(s) 473, 629, 668, 860, 955, 1020
NcoI CCATGG 1 cut(s) 478
NdeI CATATG 3 cut(s) 550, 677, 865
NdeII GATC 6 cut(s) 94, 339, 382, 553, 741, 775
NlaIII CATG 9 cut(s) 263, 367, 451, 482, 725, 752, 858, 1123, 1180
NlaIV GGNNCC 4 cut(s) 820, 832, 959, 1212
NmuCI GTSAC 1 cut(s) 583
NsiI ATGCAT 2 cut(s) 413, 856
NspI RCATGY 2 cut(s) 263, 858
PaeI GCATGC 1 cut(s) 858
PagI TCATGA 1 cut(s) 721
PceI AGGCCT 1 cut(s) 1069
PciSI GCTCTTC 1 cut(s) 1233
PctI GAATGC 1 cut(s) 282
PfeI GAWTC 3 cut(s) 240, 334, 485
PflFI GACNNNGTC 1 cut(s) 920
PfoI TCCNGGA 1 cut(s) 572
PkrI GCNGC 1 cut(s) 469
PleI GAGTC 1 cut(s) 690
PmaCI CACGTG 1 cut(s) 653
PmlI CACGTG 1 cut(s) 653
PpsI GAGTC 1 cut(s) 690
Ppu21I YACGTR 1 cut(s) 653
PpuMI RGGWCCY 2 cut(s) 716, 830
Psp5II RGGWCCY 2 cut(s) 716, 830
Psp6I CCWGG 2 cut(s) 343, 572
PspCI CACGTG 1 cut(s) 653
PspGI CCWGG 2 cut(s) 343, 572
PspN4I GGNNCC 4 cut(s) 820, 832, 959, 1212
PspPI GGNCC 4 cut(s) 127, 716, 830, 1210
PspPPI RGGWCCY 2 cut(s) 716, 830
PsyI GACNNNGTC 1 cut(s) 920
RsaI GTAC 2 cut(s) 844, 888
RsaNI GTAC 2 cut(s) 843, 887
SapI GCTCTTC 1 cut(s) 1233
SaqAI TTAA 4 cut(s) 212, 665, 1094, 1255
SatI GCNGC 1 cut(s) 468
Sau3AI GATC 6 cut(s) 94, 339, 382, 553, 741, 775
Sau96I GGNCC 4 cut(s) 127, 716, 830, 1210
ScaI AGTACT 1 cut(s) 844
SchI GAGTC 1 cut(s) 691
ScrFI CCNGG 2 cut(s) 345, 574
SduI GDGCHC 3 cut(s) 162, 960, 1245
SfaNI GCATC 5 cut(s) 420, 508, 649, 841, 1186
SfcI CTRYAG 1 cut(s) 932
SinI GGWCC 3 cut(s) 127, 716, 830
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
SphI GCATGC 1 cut(s) 858
Sse9I AATT 4 cut(s) 98, 307, 399, 1184
SseBI AGGCCT 1 cut(s) 1069
SsiI CCGC 2 cut(s) 467, 530
SspMI CTAG 2 cut(s) 200, 995
StuI AGGCCT 1 cut(s) 1069
StyD4I CCNGG 2 cut(s) 343, 572
StyI CCWWGG 2 cut(s) 478, 767
TaaI ACNGT 3 cut(s) 157, 217, 815
TaiI ACGT 6 cut(s) 306, 526, 655, 827, 1010, 1110
TaqI TCGA 1 cut(s) 685
TasI AATT 4 cut(s) 98, 307, 399, 1184
TatI WGTACW 1 cut(s) 842
TauI GCSGC 1 cut(s) 470
TfiI GAWTC 3 cut(s) 240, 334, 485
Tru1I TTAA 4 cut(s) 212, 665, 1094, 1255
Tru9I TTAA 4 cut(s) 212, 665, 1094, 1255
TscAI CASTG 5 cut(s) 162, 568, 610, 865, 1044
TseFI GTSAC 1 cut(s) 583
Tsp45I GTSAC 1 cut(s) 583
TspDTI ATGAA 5 cut(s) 17, 352, 436, 664, 1108
TspRI CASTG 5 cut(s) 162, 568, 610, 865, 1044
Tth111I GACNNNGTC 1 cut(s) 920
VpaK11BI GGWCC 3 cut(s) 127, 716, 830
XapI RAATTY 1 cut(s) 1184
XceI RCATGY 2 cut(s) 263, 858
XmiI GTMKAC 1 cut(s) 1152
XspI CTAG 2 cut(s) 200, 995
ZrmI AGTACT 1 cut(s) 844
Zsp2I ATGCAT 2 cut(s) 413, 856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.